Title:
NANOPORE SEQUENCING CIRCUIT UNIT AND GENE SEQUENCING APPARATUS
Document Type and Number:
WIPO Patent Application WO/2023/051665
Kind Code:
A1
Abstract:
A nanopore sequencing circuit unit (200) and a gene sequencing apparatus. The nanopore sequencing circuit unit (200) is implemented using a CMOS circuit, and is used for realizing the detection of a bidirectional micro-current signal of a nanopore (201). The nanopore sequencing circuit unit comprises: a nanopore clamping circuit, which is used for stabilizing the voltage of a detection electrode located on one side of the nanopore (201), generating a fixed voltage difference, across two ends of the nanopore (201), between the detection electrode and a common electrode located on the other side of the nanopore (201), and driving individual nucleotide molecules to pass through the nanopore one by one by means of the voltage difference (201); the nanopore (201) clamping circuit comprises a charging path formed by a first operational amplifier circuit (205) and a first clamping tube (214), and a discharging path formed by a second operational amplifier circuit (204) and a second clamping tube (215); an integral reset circuit, which is used for performing integral amplification on the bidirectional micro-current signal of the nanopore (201) when the charging path and the discharging path of the nanopore (201) clamping circuit are respectively connected, and converting the bidirectional micro-current signal into a voltage signal; and an output circuit, which is used for receiving the voltage signal obtained after the integral reset circuit performs conversion, and outputting the voltage signal. A high-throughput gene sequencing apparatus with a bidirectional detection capability is constructed on the basis of a small-area sequencing circuit unit, such that the detection precision and efficiency can be improved.
Inventors:
ZHANG FENGTI (CN)
JIANG KE (CN)
SU YUNPENG (CN)
ZOU YAOZHONG (CN)
JIANG KE (CN)
SU YUNPENG (CN)
ZOU YAOZHONG (CN)
Application Number:
PCT/CN2022/122448
Publication Date:
April 06, 2023
Filing Date:
September 29, 2022
Export Citation:
Assignee:
GENEUS TECH CHENGDU CO LTD (CN)
International Classes:
C12M1/34; C12Q1/6869; G01N27/327; G01N27/48
Domestic Patent References:
| WO2018156246A1 | 2018-08-30 |
Foreign References:
| CN112708544A | 2021-04-27 | |||
| CN112924745A | 2021-06-08 | |||
| US20140318964A1 | 2014-10-30 | |||
| CN112795476A | 2021-05-14 | |||
| CN105695318A | 2016-06-22 | |||
| CN111090002A | 2020-05-01 | |||
| US20120142016A1 | 2012-06-07 | |||
| CN111019814A | 2020-04-17 | |||
| US20180120288A1 | 2018-05-03 | |||
| CN112816679A | 2021-05-18 | |||
| CN103380369A | 2013-10-30 | |||
| CN107828877A | 2018-03-23 | |||
| CN103328972A | 2013-09-25 | |||
| US20170212078A1 | 2017-07-27 | |||
| CN107250780A | 2017-10-13 | |||
| CN109266537A | 2019-01-25 |
Other References:
HARTEL ANDREAS J.W.; SHEKAR SIDDHARTH; ONG PEIJIE; SCHROEDER INDRA; THIEL GERHARD; SHEPARD KENNETH L.: "High bandwidth approaches in nanopore and ion channel recordings - A tutorial review", ANALYTICA CHIMICA ACTA, vol. 1061, 1 January 1900 (1900-01-01), AMSTERDAM, NL , pages 13 - 27, XP085648729, ISSN: 0003-2670, DOI: 10.1016/j.aca.2019.01.034
JUNGSUK KIM ; RAJ MAITRA ; KENNETH D. PEDROTTI ; WILLIAM B. DUNBAR: "A Patch-Clamp ASIC for Nanopore-Based DNA Analysis", IEEE TRANSACTIONS ON BIOMEDICAL CIRCUITS AND SYSTEMS, vol. 7, no. 3, 1 June 2013 (2013-06-01), US , pages 285 - 295, XP011510705, ISSN: 1932-4545, DOI: 10.1109/TBCAS.2012.2200893
J. KIM ET AL.: "On-chip patch-clamp sensor for solid-state nanopore applications", ELECTRONICS LETTERS, 21 July 2011 (2011-07-21), pages 844 - 846, XP006039140, DOI: 10.1049/EL:20111515
SEONG-JIN KIM ; EUISIK YOON: "Label-Free CMOS Bio Sensor With On-Chip Noise Reduction Scheme for Real-Time Quantitative Monitoring of Biomolecules", IEEE TRANSACTIONS ON BIOMEDICAL CIRCUITS AND SYSTEMS, vol. 6, no. 3, 1 June 2012 (2012-06-01), US , pages 189 - 196, XP011444721, ISSN: 1932-4545, DOI: 10.1109/TBCAS.2011.2172992
BRIAN GOLDSTEIN ; DONGSOO KIM ; JIAN XU ; T. KYLE VANDERLICK ; EUGENIO CULURCIELLO: "CMOS Low Current Measurement System for Biomedical Applications", IEEE TRANSACTIONS ON BIOMEDICAL CIRCUITS AND SYSTEMS, vol. 6, no. 2, 1 April 2012 (2012-04-01), US , pages 111 - 119, XP011441870, ISSN: 1932-4545, DOI: 10.1109/TBCAS.2011.2182512
FARZIN HAQUE, JINGHONG LI, HAI-CHEN WU, XING-JIE LIANG, PEIXUAN GUO: "Solid-state and biological nanopore for real-time sensing of single chemical and sequencing of DNA", NANO TODAY, vol. 8, no. 1, 1 February 2013 (2013-02-01), NL , pages 56 - 74, XP055225044, ISSN: 1748-0132, DOI: 10.1016/j.nantod.2012.12.008
WEN CHENYU, LI SHIYU, ZENG SHUANGSHUANG, ZHANG ZHEN, ZHANG SHI-LI: "Autogenic analyte translocation in nanopores", NANO ENERGY, vol. 60, 1 June 2019 (2019-06-01), NL , pages 503 - 509, XP093053308, ISSN: 2211-2855, DOI: 10.1016/j.nanoen.2019.03.092
MA, JIAN ET AL.: "Fabrication of Si3N4 Nanopore and Detection of λ-DNA Molecules", CHINESE JOURNAL OF SENSORS AND ACTUATORS, vol. 25, no. 7, 31 July 2012 (2012-07-31), pages 876 - 879, XP009545023
WU, LINGZHI ET AL.: "Translocation of λ-DNA Through Solid-state Nanopore", JOURNAL OF NANJING UNIVERSITY OF POSTS AND TELECOMMUNICATIONS (NATURAL SCIENCE), vol. 33, no. 4, 31 August 2013 (2013-08-31), pages 115 - 118, XP009545024
"Master's Thesis", 1 January 2014, SHANGHAI JIAOTONG UNIVERSITY, CN, article WANG, YUE: "Preliminary Studies of Single-molecule DNA Sequencing Technologies Based on Nanopores and Nanoarray", pages: 28 - 43, XP009544926
LIN, YANMIN ET AL.: "Gene Sequencing Development and Its Biomedical Application", JOURNAL OF QILU INDUSTRIAL UNIVERSITY (NATURAL SCIENCE EDITION), vol. 30, no. 5, 31 December 2016 (2016-12-31), pages 24 - 28, XP009545025
JUNGSUK KIM ; RAJ MAITRA ; KENNETH D. PEDROTTI ; WILLIAM B. DUNBAR: "A Patch-Clamp ASIC for Nanopore-Based DNA Analysis", IEEE TRANSACTIONS ON BIOMEDICAL CIRCUITS AND SYSTEMS, vol. 7, no. 3, 1 June 2013 (2013-06-01), US , pages 285 - 295, XP011510705, ISSN: 1932-4545, DOI: 10.1109/TBCAS.2012.2200893
J. KIM ET AL.: "On-chip patch-clamp sensor for solid-state nanopore applications", ELECTRONICS LETTERS, 21 July 2011 (2011-07-21), pages 844 - 846, XP006039140, DOI: 10.1049/EL:20111515
SEONG-JIN KIM ; EUISIK YOON: "Label-Free CMOS Bio Sensor With On-Chip Noise Reduction Scheme for Real-Time Quantitative Monitoring of Biomolecules", IEEE TRANSACTIONS ON BIOMEDICAL CIRCUITS AND SYSTEMS, vol. 6, no. 3, 1 June 2012 (2012-06-01), US , pages 189 - 196, XP011444721, ISSN: 1932-4545, DOI: 10.1109/TBCAS.2011.2172992
BRIAN GOLDSTEIN ; DONGSOO KIM ; JIAN XU ; T. KYLE VANDERLICK ; EUGENIO CULURCIELLO: "CMOS Low Current Measurement System for Biomedical Applications", IEEE TRANSACTIONS ON BIOMEDICAL CIRCUITS AND SYSTEMS, vol. 6, no. 2, 1 April 2012 (2012-04-01), US , pages 111 - 119, XP011441870, ISSN: 1932-4545, DOI: 10.1109/TBCAS.2011.2182512
FARZIN HAQUE, JINGHONG LI, HAI-CHEN WU, XING-JIE LIANG, PEIXUAN GUO: "Solid-state and biological nanopore for real-time sensing of single chemical and sequencing of DNA", NANO TODAY, vol. 8, no. 1, 1 February 2013 (2013-02-01), NL , pages 56 - 74, XP055225044, ISSN: 1748-0132, DOI: 10.1016/j.nantod.2012.12.008
WEN CHENYU, LI SHIYU, ZENG SHUANGSHUANG, ZHANG ZHEN, ZHANG SHI-LI: "Autogenic analyte translocation in nanopores", NANO ENERGY, vol. 60, 1 June 2019 (2019-06-01), NL , pages 503 - 509, XP093053308, ISSN: 2211-2855, DOI: 10.1016/j.nanoen.2019.03.092
MA, JIAN ET AL.: "Fabrication of Si3N4 Nanopore and Detection of λ-DNA Molecules", CHINESE JOURNAL OF SENSORS AND ACTUATORS, vol. 25, no. 7, 31 July 2012 (2012-07-31), pages 876 - 879, XP009545023
WU, LINGZHI ET AL.: "Translocation of λ-DNA Through Solid-state Nanopore", JOURNAL OF NANJING UNIVERSITY OF POSTS AND TELECOMMUNICATIONS (NATURAL SCIENCE), vol. 33, no. 4, 31 August 2013 (2013-08-31), pages 115 - 118, XP009545024
"Master's Thesis", 1 January 2014, SHANGHAI JIAOTONG UNIVERSITY, CN, article WANG, YUE: "Preliminary Studies of Single-molecule DNA Sequencing Technologies Based on Nanopores and Nanoarray", pages: 28 - 43, XP009544926
LIN, YANMIN ET AL.: "Gene Sequencing Development and Its Biomedical Application", JOURNAL OF QILU INDUSTRIAL UNIVERSITY (NATURAL SCIENCE EDITION), vol. 30, no. 5, 31 December 2016 (2016-12-31), pages 24 - 28, XP009545025
Attorney, Agent or Firm:
CHENG & PENG INTELLECTUAL PROPERTY LAW OFFICE (CN)
Download PDF:
Previous Patent: IMAGE PROCESSING METHOD AND APPARATUS
Next Patent: ULTRA-SMALL AREA MICRO-CURRENT DETECTION CIRCUIT UNIT AND SYSTEM
Next Patent: ULTRA-SMALL AREA MICRO-CURRENT DETECTION CIRCUIT UNIT AND SYSTEM