Title:
ULTRA-SMALL AREA MICRO-CURRENT DETECTION CIRCUIT UNIT AND SYSTEM
Document Type and Number:
WIPO Patent Application WO/2023/051666
Kind Code:
A1
Abstract:
A nanopore sequencing circuit unit (200) and a gene sequencing device. The nanopore sequencing circuit unit (200) is implemented by using a CMOS circuit, and is used for detecting a bidirectional micro-current signal of a nanopore (201). The circuit unit comprises: a nanopore clamping circuit, which is used for stabilizing the voltage of a detection electrode positioned on one side of the nanopore (201), so as to generate a fixed voltage difference with a common electrode (202) positioned on the other side of the nanopore (201), and to form a charging and discharging path with an integral reset circuit, driving single nucleotide molecules to pass through the nanopore (201) one by one by means of the voltage difference; the integral reset circuit, which is used for, when the nanopore clamping circuit constitutes the charging and discharging path, performing integral amplification on a bidirectional micro-current signal of the nanopore (201) , and converting the bidirectional micro-current signal into a voltage signal; and an output circuit, which is used for receiving the voltage signal converted by the integral reset circuit, and outputting the voltage signal. A high-throughput gene sequencing device having bidirectional detection capability is constructed on the basis of a small-area sequencing circuit unit, such that the detection precision and efficiency are improved.
Inventors:
ZHANG FENGTI (CN)
JIANG KE (CN)
SU YUNPENG (CN)
ZOU YAOZHONG (CN)
JIANG KE (CN)
SU YUNPENG (CN)
ZOU YAOZHONG (CN)
Application Number:
PCT/CN2022/122449
Publication Date:
April 06, 2023
Filing Date:
September 29, 2022
Export Citation:
Assignee:
GENEUS TECH CHENGDU CO LTD (CN)
International Classes:
C12M1/34; C12Q1/6869; G01N27/327; G01N27/48
Domestic Patent References:
| WO2018156246A1 | 2018-08-30 |
Foreign References:
| CN112708544A | 2021-04-27 | |||
| CN112924745A | 2021-06-08 | |||
| CN112795476A | 2021-05-14 | |||
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| US20140318964A1 | 2014-10-30 | |||
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| US20180120288A1 | 2018-05-03 | |||
| US20120142016A1 | 2012-06-07 | |||
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| CN103380369A | 2013-10-30 | |||
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| CN109266537A | 2019-01-25 |
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WU, LINGZHI ET AL.: "Translocation of λ-DNA Through Solid-state Nanopore", JOURNAL OF NANJING UNIVERSITY OF POSTS AND TELECOMMUNICATIONS (NATURAL SCIENCE), vol. 33, no. 4, 31 August 2013 (2013-08-31), pages 115 - 118, XP009545024
WANG, YUE: "Preliminary Studies of Single-molecule DNA Sequencing Technologies Based on Nanopores and Nanoarray", CHINESE MASTER’S THESES FULL-TEXT DATABASE BASIC SCIENCES, no. 6, 15 June 2015 (2015-06-15), pages 28 - 43
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JUNGSUK KIM ; RAJ MAITRA ; KENNETH D. PEDROTTI ; WILLIAM B. DUNBAR: "A Patch-Clamp ASIC for Nanopore-Based DNA Analysis", IEEE TRANSACTIONS ON BIOMEDICAL CIRCUITS AND SYSTEMS, vol. 7, no. 3, 1 June 2013 (2013-06-01), US , pages 285 - 295, XP011510705, ISSN: 1932-4545, DOI: 10.1109/TBCAS.2012.2200893
J. KIM ET AL.: "On-chip Patch-clamp Sensor for Solid-state Nanopore Applications", ELECTRONICS LETTERS, vol. 47, no. 15, 21 July 2011 (2011-07-21), pages 844 - 846, XP006039140, DOI: 10.1049/EL:20111515
SEONG-JIN KIM ; EUISIK YOON: "Label-Free CMOS Bio Sensor With On-Chip Noise Reduction Scheme for Real-Time Quantitative Monitoring of Biomolecules", IEEE TRANSACTIONS ON BIOMEDICAL CIRCUITS AND SYSTEMS, vol. 6, no. 3, 1 June 2012 (2012-06-01), US , pages 189 - 196, XP011444721, ISSN: 1932-4545, DOI: 10.1109/TBCAS.2011.2172992
BRIAN GOLDSTEIN ; DONGSOO KIM ; JIAN XU ; T. KYLE VANDERLICK ; EUGENIO CULURCIELLO: "CMOS Low Current Measurement System for Biomedical Applications", IEEE TRANSACTIONS ON BIOMEDICAL CIRCUITS AND SYSTEMS, vol. 6, no. 2, 1 April 2012 (2012-04-01), US , pages 111 - 119, XP011441870, ISSN: 1932-4545, DOI: 10.1109/TBCAS.2011.2182512
FARZIN HAQUE ET AL.: "Solid-state and Biological Nanopore for Real-time Sensing of Single Chemical and Sequencing of DNA", NANO TODAY, vol. 8, no. 1, 28 February 2013 (2013-02-28), pages 56 - 74, XP055225044, DOI: 10.1016/j.nantod.2012.12.008
WEN CHENYU, LI SHIYU, ZENG SHUANGSHUANG, ZHANG ZHEN, ZHANG SHI-LI: "Autogenic analyte translocation in nanopores", NANO ENERGY, vol. 60, 1 June 2019 (2019-06-01), NL , pages 503 - 509, XP093053308, ISSN: 2211-2855, DOI: 10.1016/j.nanoen.2019.03.092
MA, JIAN ET AL.: "Fabrication of Si3N4 Nanopore and Detection of λ-DNA Molecules", CHINESE JOURNAL OF SENSORS AND ACTUATORS, vol. 25, no. 7, 31 July 2012 (2012-07-31), pages 876 - 879, XP009545023
WU, LINGZHI ET AL.: "Translocation of λ-DNA Through Solid-state Nanopore", JOURNAL OF NANJING UNIVERSITY OF POSTS AND TELECOMMUNICATIONS (NATURAL SCIENCE), vol. 33, no. 4, 31 August 2013 (2013-08-31), pages 115 - 118, XP009545024
WANG, YUE: "Preliminary Studies of Single-molecule DNA Sequencing Technologies Based on Nanopores and Nanoarray", CHINESE MASTER’S THESES FULL-TEXT DATABASE BASIC SCIENCES, no. 6, 15 June 2015 (2015-06-15), pages 28 - 43
LIN, ZIHAN ET AL.: "Nanopore-Based Biomolecular Detection", PROGRESS IN CHEMISTRY, vol. 32, no. 5, 31 December 2020 (2020-12-31), pages 562 - 580, XP009544851
LIN, YANMIN ET AL.: "Gene Sequencing Development and Its Biomedical Application", JOURNAL OF QILU INDUSTRIAL UNIVERSITY (NATURAL SCIENCE EDITION), vol. 30, no. 5, 31 December 2016 (2016-12-31), pages 24 - 28, XP009545025
Attorney, Agent or Firm:
CHENG & PENG INTELLECTUAL PROPERTY LAW OFFICE (CN)
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