Title:
NITRILE HYDRATASE VARIANT
Document Type and Number:
WIPO Patent Application WO/2010/055666
Kind Code:
A1
Abstract:
The nitrile hydratase variant involves substitution of at least one amino acid with another amino acid to improve two or more properties of the nitrile hydratase by the substitution of one amino acid.
Inventors:
MATSUMOTO KAZUYA (JP)
KAZUNO YASUSHI (JP)
MOCHIZUKI DAISUKE (JP)
TOKUDA JUNKO (JP)
KAZUNO YASUSHI (JP)
MOCHIZUKI DAISUKE (JP)
TOKUDA JUNKO (JP)
Application Number:
PCT/JP2009/006055
Publication Date:
May 20, 2010
Filing Date:
November 12, 2009
Export Citation:
Assignee:
MITSUI CHEMICALS INC (JP)
MATSUMOTO KAZUYA (JP)
KAZUNO YASUSHI (JP)
MOCHIZUKI DAISUKE (JP)
TOKUDA JUNKO (JP)
MATSUMOTO KAZUYA (JP)
KAZUNO YASUSHI (JP)
MOCHIZUKI DAISUKE (JP)
TOKUDA JUNKO (JP)
International Classes:
C12N15/09; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/88
Domestic Patent References:
| WO2005116206A1 | 2005-12-08 | |||
| WO2004056990A1 | 2004-07-08 |
Foreign References:
| JP2008253182A | 2008-10-23 | |||
| JPH09275978A | 1997-10-28 | |||
| JP2005160403A | 2005-06-23 | |||
| JP2004194588A | 2004-07-15 | |||
| JPH09275978A | 1997-10-28 | |||
| JP2004194588A | 2004-07-15 | |||
| JP2005160403A | 2005-06-23 | |||
| JP2007143409A | 2007-06-14 | |||
| JP2008253182A | 2008-10-23 | |||
| JPH11253168A | 1999-09-21 |
Other References:
MIYANAGA A. ET AL.: "Mutational and structural analysis of cobalt-containing nitrile hydratase on substrate and metal binding", EUR. J. BIOCHEM., vol. 271, no. 2, 2004, pages 429 - 438, XP055096861
J. SAMBROOK ET AL.: "Molecular Cloning", 2001, COLD SPRING HARBOR LABORATORY PRESS
J. SAMBROOK ET AL.: "Molecular Cloning", 2001, COLD SPRING HARBOR LABORATORY PRESS
Attorney, Agent or Firm:
HAYAMI, Shinji et al. (JP)
Shinji Hayami (JP)
Shinji Hayami (JP)
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