Title:
NLK AS A MARKER FOR DIAGNOSIS OF LIVER CANCER AND AS A THERAPEUTIC AGENT THEREOF
Document Type and Number:
WIPO Patent Application WO/2011/118994
Kind Code:
A2
Abstract:
The present invention relates to a novel marker for the diagnosis of liver cancer and the use thereof. More specifically, the present invention relates to a marker for the diagnosis of liver cancer, using NLK (nemo like kinase) by confirming the overexpression of the NLK in the liver cancer cells, and to a composition, a kit, a microarray, and a method for the diagnosis of liver cancer using the marker. The present invention also relates to a method for screening a substance that can prevent or treat liver cancer by reducing the expression of a marker gene or a protein, and to a composition for preventing or treating liver cancer, which includes said substance. According to the present invention, NLK, which is the marker gene for liver cancer, has an unusually increased amount of expression in the liver cancer tissues compared to the normal liver tissues, and thus suppressing the expression of said gene reduces the expression of cyclin D1 and CDK2 in liver cancer cells, suppresses the progression of a cell cycle in stage G1/S, and at the same time, restrains the proliferation of the cells, thereby effectively preventing or treating liver cancer. Therefore, the NLK gene discovered in the current invention can be usefully used as a target for the diagnosis and the treatment of liver cancer.
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Inventors:
NAM SUK WOO (KR)
Application Number:
PCT/KR2011/002031
Publication Date:
September 29, 2011
Filing Date:
March 25, 2011
Export Citation:
Assignee:
CATHOLIC UNIV IND ACAD COOP (KR)
NAM SUK WOO (KR)
NAM SUK WOO (KR)
International Classes:
C12Q1/68; A61K48/00; A61P35/00; C12N15/113; G01N33/574
Other References:
YASUDA, J. ET AL.: 'Nemo-like kinase induces apoptosis in DLD-1 human colon cancer cells' BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. vol. 308, no. 2, 22 August 2003, pages 227 - 233
EMAMI, K. H. ET AL.: 'Nemo-like kinase induces apoptosis and inhibits androgen receptor signaling in prostate cancer cells' PROSTATE. vol. 69, no. 14, 01 October 2009, pages 1481 - 1492
JI, J. ET AL.: 'Identification of microRNA-181 by genome-wide screening as a critical player in EpCAM-positive hepatic cancer stem cells' HEPATOLOGY. vol. 50, no. 2, August 2009, pages 472 - 480
JUNG, K. H. ET AL.: 'Targeted disruption of Nemo-like kinase inhibits tumor Cell growth by simultaneous suppression of cyclin D and CDK2 in human hepatocellular carcinoma' JOURNAL OF CELLULAR BIOCHEMISTRY vol. 110, no. 3, 01 June 2010, pages 687 - 696
EMAMI, K. H. ET AL.: 'Nemo-like kinase induces apoptosis and inhibits androgen receptor signaling in prostate cancer cells' PROSTATE. vol. 69, no. 14, 01 October 2009, pages 1481 - 1492
JI, J. ET AL.: 'Identification of microRNA-181 by genome-wide screening as a critical player in EpCAM-positive hepatic cancer stem cells' HEPATOLOGY. vol. 50, no. 2, August 2009, pages 472 - 480
JUNG, K. H. ET AL.: 'Targeted disruption of Nemo-like kinase inhibits tumor Cell growth by simultaneous suppression of cyclin D and CDK2 in human hepatocellular carcinoma' JOURNAL OF CELLULAR BIOCHEMISTRY vol. 110, no. 3, 01 June 2010, pages 687 - 696
Attorney, Agent or Firm:
WIE, Byoung-Gab et al. (KR)
위병갑 (KR)
위병갑 (KR)
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Claims: