Title:
NON-PHOSPHORYLATED AND NON-UBIQUITINATED CREPT PROTEIN AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2024/032485
Kind Code:
A1
Abstract:
The present invention relates to a non-phosphorylated and non-ubiquitinated CREPT protein and use thereof. Specifically, the present invention relates to a protein that is obtained by modifying CREPT or a homologous protein thereof. The modification enables the modified protein to maintain a non-phosphorylation and non-ubiquitination state so as not to be degraded when the protein is located in eukaryotic cells at the end of the G1 phase or during the G1/S transition phase, so that a double-MCM hexamer cannot be separated, thereby stopping the cell cycle, generating a genome stress response, and finally causing cell death. The present invention also relates to a method for screening a non-phosphorylated and non-ubiquitinated modifier of CREPT, a method for identifying whether a substance is a phosphorylation inhibitor of CREPT, a method for identifying eukaryotic cells at the end of the G1 phase or during the G1/S transition phase by using CREPT, and a method for inducing cancer cell apoptosis to treat cancers on the basis of non-phosphorylated and non-ubiquitinated CREPT.
Inventors:
CHANG ZHIJIE (CN)
REN FANGLI (CN)
WANG YINYIN (CN)
SONG YUNHAO (CN)
REN FANGLI (CN)
WANG YINYIN (CN)
SONG YUNHAO (CN)
Application Number:
PCT/CN2023/111172
Publication Date:
February 15, 2024
Filing Date:
August 04, 2023
Export Citation:
Assignee:
UNIV TSINGHUA (CN)
International Classes:
C07K14/82; A61K38/16; A61K48/00; A61P35/00; C07K1/107; C12N5/10; C12N15/113; C12N15/12; C12N15/65; C12N15/85; G01N33/53; G01N33/539; G01N33/68
Foreign References:
| CN116143907A | 2023-05-23 | |||
| CN108949718A | 2018-12-07 | |||
| CN1807623A | 2006-07-26 | |||
| CN109295000A | 2019-02-01 |
Other References:
DING LIDAN, YANG LIU, HE YUQI, ZHU BINGTAO, REN FANGLI, FAN XUANZI, WANG YINYIN, LI MENGDI, LI JUN, KUANG YANSHEN, LIU SIHAN, ZHAI: "CREPT/RPRD1B associates with Aurora B to regulate Cyclin B1 expression for accelerating the G2/M transition in gastric cancer", CELL DEATH & DISEASE, NATURE PUBLISHING GROUP, GB, vol. 9, no. 12, 5 December 2018 (2018-12-05), GB , pages 1172, XP093137885, ISSN: 2041-4889, DOI: 10.1038/s41419-018-1211-8
Attorney, Agent or Firm:
BEIJING SANYOU INTELLECTUAL PROPERTY AGENCY LTD. (CN)
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