Title:
A NOVEL GENERATION OF CLONED HORSESHOE CRAB RECOMBINANT FACTOR C FOR DETECTION AND REMOVAL OF ENDOTOXIN
Document Type and Number:
WIPO Patent Application WO1999015676
Kind Code:
B1
Abstract:
The horseshoe crab, Carcinoscropius rotundicauda Factor C cDNA (CrFC21) has been cloned into a shuttle baculoviral vector. The recombinant baculoviral DNA was then transfected into the insect cells for expression of recombinant Factor C. Recombinant Factor C was found to be immunoreactive and is capable of binding both free and bound/immobilized lipid A. It is enzymatically active when triggered by LPS. The rFC is probably of the two-chain form, being cleaved into the heavy and light chains after activation by Gram negative bacterial endotoxin. As low as 0.01 pg (0.001 ng/ml) of LPS was detectable by the rFC, thus, indicating its potentials as a novel generation of "limulus amoebocyte lysate".
Inventors:
DING JEAK LING
HO BOW
HO BOW
Application Number:
PCT/SG1998/000073
Publication Date:
May 14, 1999
Filing Date:
September 18, 1998
Export Citation:
Assignee:
UNIV SINGAPORE (SG)
International Classes:
C07K14/435; C12N15/55; G01N33/579; (IPC1-7): C12N15/63; C12N15/55; C12Q1/37
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