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Title:
NOVEL HEXENURONIDASE, GENE CODING FOR THE SAME, AND USE OF THESE
Document Type and Number:
WIPO Patent Application WO/2002/092809
Kind Code:
A1
Abstract:
A novel hexenuronidase characterized in that it has hexenuronidase activity and a molecular weight of about 40,000 to 45,000 and the enzymatic activity is enhanced by 2-mercaptoethanol or dithiothreitol; a process for producing the hexenuronidase; a use of the hexenuronidase; a hexenuroidase gene; and a microorganism which yields the hexenuroidase. This enzymatic protein is useful in bleaching a pulp.

Inventors:
IZUMI YOSHIYA (JP)
SAKAINO MAKOTO (JP)
TSUKAMOTO AKIRA (JP)
Application Number:
PCT/JP2002/004390
Publication Date:
November 21, 2002
Filing Date:
May 02, 2002
Export Citation:
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Assignee:
OJI PAPER CO (JP)
IZUMI YOSHIYA (JP)
SAKAINO MAKOTO (JP)
TSUKAMOTO AKIRA (JP)
International Classes:
C12N15/09; C07K16/40; C12N1/15; C12N1/19; C12N1/20; C12N1/21; C12N5/10; C12N9/14; C12N9/42; D21C5/00; D21C9/10; (IPC1-7): C12N15/09; C07K16/40; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/14; D21C9/147
Domestic Patent References:
WO1995033883A11995-12-14
WO1993011296A11993-06-10
Foreign References:
EP0355831A21990-02-28
Other References:
HASHIMOTO W. ET AL.: "Unsaturated glucuronyl hydrolase of Bacillus sp.GL1: Novel enzyme prerequisite for metabolism of unsaturated oligosaccharides produced by polysaccharide lyases", ARCH. BIOCHEM. BIOPHYS., vol. 368, no. 2, 1999, pages 367 - 374, XP002956617
CHENG F. ET AL.: "Variations in the chondroitin sulfate-protein linkage region of aggrecans from bovine nasal and human articular cartilages", J. BIOL. CHEM., vol. 271, no. 45, 1996, pages 28572 - 28580, XP002956618
HOVINGH P. ET AL.: "Specificity of flavobacterial glycuronidases acting on disaccharides derived from glycosaminoglycans", BIOCHEM. J., vol. 165, no. 2, 1977, pages 287 - 293, XP002956619
Attorney, Agent or Firm:
Hiraki, Yusuke (17-1 Toranomon 1-chom, Minato-ku Tokyo, JP)
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