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Title:
NOVEL METHOD OF NUCLEIC ACID TRANSFER
Document Type and Number:
WIPO Patent Application WO/2005/061717
Kind Code:
A1
Abstract:
It is intended to provide a method of transferring a nucleic acid which comprises: (a) the step of contacting the nucleic acid with a cell in a medium; and (b) the step of, after the completion of the above step (a), contacting a metal salt solution of a high concentration with the medium in the step (a); an agent for transferring a nucleic acid which contains a solid metal salt or a metal salt solution of a high concentration as a component thereof; and so on.

Inventors:
MINAKUCHI YOSHIKO (JP)
OCHIYA TAKAHIRO (JP)
Application Number:
PCT/JP2004/019160
Publication Date:
July 07, 2005
Filing Date:
December 15, 2004
Export Citation:
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Assignee:
SUMITOMO PHARMA (JP)
KOKEN KK (JP)
MINAKUCHI YOSHIKO (JP)
OCHIYA TAKAHIRO (JP)
International Classes:
C12N15/11; C12N15/113; C12N15/63; (IPC1-7): C12N15/63
Domestic Patent References:
WO2000073414A12000-12-07
WO2001097857A12001-12-27
Foreign References:
JP2002325572A2002-11-12
JP2001335512A2001-12-04
JPH01157388A1989-06-20
JPH0491783A1992-03-25
JP2003274950A2003-09-30
Other References:
HONMA K. ET AL: "Atelocollagen-based gene transfer in cells allows high-throughput screening of gene functions", BIOCHEM.BIOPHYS.RES.COMMUN., vol. 289, no. 5, 2001, pages 1075 - 1081, XP002957375
OCHIYA T. ET AL: "Tennen Kobunshi ni yoru Idenshi Vector no Atarashii Unpan System", EXPERIMENTAL MEDICINE, vol. 17, no. 17, 1999, pages 2288 - 2291
Attorney, Agent or Firm:
Isobe, Yutaka (Dainippon Sumitomo Pharma Co. Ltd., 1-98, Kasugadenaka 3-chome, Konohana-k, Osaka-shi Osaka 22, JP)
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