Title:
NOVEL MICROORGNAISM SENSITIVE TO LYSOZYME
Document Type and Number:
WIPO Patent Application WO/2004/018651
Kind Code:
A1
Abstract:
It is intended to provide a bacterium belonging to the genus Rhodococcus which is sensitive to lysozyme at a low concentration compared with a wild type strain, can be easily lysed and enables the recovery of an expressed recombinant protein. Namely, a mutant microorganism belonging to the genus Rhodococcus which has a higher sensitivity to lysozyme than that of a wild type microorganism belonging to the genus Rhodococcus.
Inventors:
MITANI YASUO (JP)
NAKASHIMA NOBUTAKA (JP)
TAMURA TOMOHIRO (JP)
NAKASHIMA NOBUTAKA (JP)
TAMURA TOMOHIRO (JP)
Application Number:
PCT/JP2003/010342
Publication Date:
March 04, 2004
Filing Date:
August 14, 2003
Export Citation:
Assignee:
NAT INST OF ADVANCED IND SCIEN (JP)
MITANI YASUO (JP)
NAKASHIMA NOBUTAKA (JP)
TAMURA TOMOHIRO (JP)
MITANI YASUO (JP)
NAKASHIMA NOBUTAKA (JP)
TAMURA TOMOHIRO (JP)
International Classes:
C12N1/20; C12N1/21; C12P21/02; C12R1/01; (IPC1-7): C12N1/20; C12N1/21; C12P21/02
Foreign References:
| EP0064680A2 | 1982-11-17 | |||
| JPS61280273A | 1986-12-10 | |||
| JPH07255484A | 1995-10-09 |
Other References:
BAILEY S.A. ET AL.: "Emulsification of crude oil by rhodococcus erythropolis strain ST-2 via a cell-surface, lysozyme-sensitive glycoprotein", SYSTEM APPL. MICROBIOL., vol. 20, 1997, pages 545 - 548, XP002973368
INOUE M. ET AL.: "Isolation and characterization of lysozyme-sensitive mutants of staphylococcus aureus", J. BACTERIOL., vol. 144, no. 3, 1980, pages 1186 - 1189, XP002973369
HIRASAWA T. ET AL.: "A mutation in the coryne-bacterium glutamicum ltsA gene casuses susceptibility to lysozyme, temperature-sensitive growth and L-glutamate production", J. BACTERIOL., vol. 182, no. 10, 2000, pages 2696 - 2701, XP002973370
INOUE M. ET AL.: "Isolation and characterization of lysozyme-sensitive mutants of staphylococcus aureus", J. BACTERIOL., vol. 144, no. 3, 1980, pages 1186 - 1189, XP002973369
HIRASAWA T. ET AL.: "A mutation in the coryne-bacterium glutamicum ltsA gene casuses susceptibility to lysozyme, temperature-sensitive growth and L-glutamate production", J. BACTERIOL., vol. 182, no. 10, 2000, pages 2696 - 2701, XP002973370
Attorney, Agent or Firm:
Hiraki, Yusuke (17-1 Toranomon 1-chom, Minato-ku Tokyo, JP)
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