Title:
NOVEL MONOCLONAL ANTIBODY AND USE THEREOF AS TAG ANTIBODY
Document Type and Number:
WIPO Patent Application WO/2012/124683
Kind Code:
A1
Abstract:
Provided is a novel monoclonal antibody which can be widely used as a tag antibody in an immunodetection method using an antigen-antibody reaction such as a Western blotting method, an immunoprecipitation method, a chromatin immunoprecipitation method, and an immunostaining method.
The monoclonal antibody is a monoclonal antibody specifically binding to a peptide or a protein containing an amino acid sequence represented by SEQ ID NO: 1 as an epitope region, and is characterized by comprising a heavy chain variable region containing CDR1 comprising the amino acid sequence of amino acid numbers 18 to 25 in SEQ ID NO: 2, CDR2 comprising the amino acid sequence of amino acid numbers 43 to 50 in SEQ ID NO: 2, and CDR3 comprising the amino acid sequence of amino acid numbers 89 to 97 in SEQ ID NO: 2, and a light chain variable region containing CDR1 comprising the amino acid sequence of amino acid numbers 18 to 23 in SEQ ID NO: 3, CDR2 comprising the amino acid sequence of amino acid numbers 41 to 43 in SEQ ID NO: 3, and CDR3 comprising the amino acid sequence of amino acid numbers 80 to 88 in SEQ ID NO: 3.
Inventors:
URANO TAKESHI (JP)
TATSUMI KASUMI (JP)
SEKINE JOJI (JP)
TATSUMI KASUMI (JP)
SEKINE JOJI (JP)
Application Number:
PCT/JP2012/056384
Publication Date:
September 20, 2012
Filing Date:
March 13, 2012
Export Citation:
Assignee:
NAT UNIV CORP SHIMANE UNIV (JP)
URANO TAKESHI (JP)
TATSUMI KASUMI (JP)
SEKINE JOJI (JP)
URANO TAKESHI (JP)
TATSUMI KASUMI (JP)
SEKINE JOJI (JP)
International Classes:
C12N15/09; C07K7/06; C07K16/00; C12N15/02; G01N33/536; C12P21/08
Foreign References:
| JP2005326165A | 2005-11-24 |
Other References:
LIU J. ET AL.: "pPIC9-Fc: A Vector System for the Production of Single-Chain Fv-Fc Fusions in Pichia pastoris as Detection Reagents In Vitro", J. BIOCHEM., vol. 134, 2003, pages 911 - 917
JURONEN E. ET AL.: "Production and Characterization of Monoclonal Antibodies against Class Theta Glutathione S-Transferase T1-1", HYBRIDOMA, vol. 15, no. 1, 1996, pages 77 - 82
VINOKUROV L. M. ET AL.: "Stability of functionally active fusion proteins during their biosynthesis and isolation from expressing bacterial cells", PROGRESS IN BIOTECHNOLOGY, vol. 15, 1998, pages 645 - 650
JURONEN E. ET AL.: "Production and Characterization of Monoclonal Antibodies against Class Theta Glutathione S-Transferase T1-1", HYBRIDOMA, vol. 15, no. 1, 1996, pages 77 - 82
VINOKUROV L. M. ET AL.: "Stability of functionally active fusion proteins during their biosynthesis and isolation from expressing bacterial cells", PROGRESS IN BIOTECHNOLOGY, vol. 15, 1998, pages 645 - 650
Attorney, Agent or Firm:
SAEGUSA & PARTNERS (JP)
Patent business corporation 3 Edakuni [Hajime] patent firm (JP)
Patent business corporation 3 Edakuni [Hajime] patent firm (JP)
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