Title:
NOVEL NITRILE HYDRATASE
Document Type and Number:
WIPO Patent Application WO/2004/108942
Kind Code:
A1
Abstract:
A process for producing an amide compound with the use of a nitrile hydratase of high thermal stability capable of maintaining high activity even in the presence of high-concentration nitrile compound as a substrate and amide compound as a product. A recombinant microbe was produced by searching for a novel microbe capable of expression of the desired nitrile hydratase in nature, obtaining relevant gene from cells of the microbe or treatment product thereof, performing cloning of the gene and introducing the same in a different type of microbe for expression. With the use thereof, nitrile compounds can be efficiently converted to corresponding amide compounds even by the reaction conducted at high temperature and in the presence of high-concentration nitrile compounds and high-concentration amide compounds.
Inventors:
FURUYA KAORU (JP)
TAMAKI AKIRA (JP)
NAGASAWA SHIN-ICHIRO (JP)
SUZUKI AYANO (JP)
TAMAKI AKIRA (JP)
NAGASAWA SHIN-ICHIRO (JP)
SUZUKI AYANO (JP)
Application Number:
PCT/JP2004/008515
Publication Date:
December 16, 2004
Filing Date:
June 10, 2004
Export Citation:
Assignee:
ASAHI CHEMICAL IND (JP)
FURUYA KAORU (JP)
TAMAKI AKIRA (JP)
NAGASAWA SHIN-ICHIRO (JP)
SUZUKI AYANO (JP)
FURUYA KAORU (JP)
TAMAKI AKIRA (JP)
NAGASAWA SHIN-ICHIRO (JP)
SUZUKI AYANO (JP)
International Classes:
C12N9/88; (IPC1-7): C12P13/02; C12N1/20; C12N15/09
Domestic Patent References:
| WO1999055719A1 | 1999-11-04 |
Attorney, Agent or Firm:
SIKs & Co. (8-7 Kyobashi 1-chome, Chuo-k, Tokyo 31, JP)
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