Title:
NOVEL PIGGYBAC TRANSPOSON SYSTEM AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2022/078310
Kind Code:
A1
Abstract:
Provided is a nucleic acid construct, comprising the following elements: a transposon 3' terminal repeat, a first polyA sequence, an insulator sequence having a transcription termination function, a transposon 5' terminal repeat, a transposase coding sequence, and a promoter that controls the expression of the transposase. Also provided are a host cell or pharmaceutical composition comprising said nucleic acid construct, and a use thereof.
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Inventors:
JIN HUAJUN (CN)
XU FUHUI (CN)
HUANG CHEN (CN)
MA XINGMING (CN)
LIU TIANYI (CN)
GUO XIAOCHUN (CN)
XU FUHUI (CN)
HUANG CHEN (CN)
MA XINGMING (CN)
LIU TIANYI (CN)
GUO XIAOCHUN (CN)
Application Number:
PCT/CN2021/123191
Publication Date:
April 21, 2022
Filing Date:
October 12, 2021
Export Citation:
Assignee:
SHANGHAI GENCELLS THERAPEUTICS CO LTD (CN)
International Classes:
C12N15/63; A61K48/00; A61P43/00
Domestic Patent References:
| WO2017046259A1 | 2017-03-23 | |||
| WO2019046815A1 | 2019-03-07 |
Foreign References:
| CN105154473A | 2015-12-16 | |||
| US20040197910A1 | 2004-10-07 | |||
| US20040197910A1 | 2004-10-07 | |||
| CN107523549A | 2017-12-29 | |||
| CN102943092A | 2013-02-27 | |||
| CN105154473A | 2015-12-16 |
Other References:
"Thesis Bei Nong Bo Xidu University", 15 February 2015, BEI NONG BO XIDU UNIVERSITY, CN, article HUANG HUI: "Construction of a PiggyBac Transposon(PB) Inducible Cell Immortalization Vector and Its Verification in BMECs", pages: 1 - 57, XP055923496
DING SHENG: "Sleeping Beauty Transposition System", SHENGWU HUAXUE YU SHENGWU WULI JINZHAN - BIOCHEMISTRY AND BIOPHYSICS, KEXUE CHUBANSHE, BEIJING, CN, vol. 30, no. 1, 1 January 2003 (2003-01-01), CN , pages 43 - 48, XP055923505, ISSN: 1000-3282
ZHAO SHUANG, JIANG ENZE, CHEN SHUANGSHUANG, GU YUAN, SHANGGUAN ANNA JUNJIE, LV TANGFENG, LUO LIGUO, YU ZHENGHONG: "PiggyBac transposon vectors: the tools of the human gene encoding", TRANSLATIONAL LUNG CANCER RESEARCH, SOCIETY FOR TRANSLATIONAL CANCER RESEARCH (STCR), HONG KONG, vol. 5, no. 1, 1 February 2016 (2016-02-01), Hong Kong , pages 120 - 125, XP055923508, ISSN: 2218-6751, DOI: 10.3978/j.issn.2218-6751.2016.01.05
SAMBROOK ET AL.: "Molecular Cloning: A Laboratory Manual", 2001, COLD SPRING HARBOR LABORATORY
J.SAMBROOK ET AL.: "Molecular Cloning Experiment Guide", SCIENCE PRESS
DING SHENG: "Sleeping Beauty Transposition System", SHENGWU HUAXUE YU SHENGWU WULI JINZHAN - BIOCHEMISTRY AND BIOPHYSICS, KEXUE CHUBANSHE, BEIJING, CN, vol. 30, no. 1, 1 January 2003 (2003-01-01), CN , pages 43 - 48, XP055923505, ISSN: 1000-3282
ZHAO SHUANG, JIANG ENZE, CHEN SHUANGSHUANG, GU YUAN, SHANGGUAN ANNA JUNJIE, LV TANGFENG, LUO LIGUO, YU ZHENGHONG: "PiggyBac transposon vectors: the tools of the human gene encoding", TRANSLATIONAL LUNG CANCER RESEARCH, SOCIETY FOR TRANSLATIONAL CANCER RESEARCH (STCR), HONG KONG, vol. 5, no. 1, 1 February 2016 (2016-02-01), Hong Kong , pages 120 - 125, XP055923508, ISSN: 2218-6751, DOI: 10.3978/j.issn.2218-6751.2016.01.05
SAMBROOK ET AL.: "Molecular Cloning: A Laboratory Manual", 2001, COLD SPRING HARBOR LABORATORY
J.SAMBROOK ET AL.: "Molecular Cloning Experiment Guide", SCIENCE PRESS
Attorney, Agent or Firm:
SHANGHAI PATENT & TRADEMARK LAW OFFICE, LLC (CN)
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