Title:
NOVEL PROCESS FOR PURIFYING HEPARAN-N-SULFATASE
Document Type and Number:
WIPO Patent Application WO/2024/063469
Kind Code:
A1
Abstract:
The present invention is related to a method for purifying heparan-N-sulfatase from a heparan-N-sulfatase-containing solution including at least one impurity, the method comprising performing multi-mode chromatography (MMC) to obtain an eluate; and performing caprylate precipitation to obtain a supernatant. The method according to the present invention is capable of very efficiently removing HCP (host cell proteins) and of greatly improving the purity and stability of purified heparan-N-sulfatase.
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Inventors:
KO KWANYOUNG (KR)
KIM DASOM (KR)
YOO SULGI (KR)
YEO GEUNHYE (KR)
KIM MINJUNG (KR)
JIN DONG KYU (KR)
KIM DASOM (KR)
YOO SULGI (KR)
YEO GEUNHYE (KR)
KIM MINJUNG (KR)
JIN DONG KYU (KR)
Application Number:
PCT/KR2023/014028
Publication Date:
March 28, 2024
Filing Date:
September 18, 2023
Export Citation:
Assignee:
GREEN CROSS CORP (KR)
NOVEL PHARMA INC (KR)
NOVEL PHARMA INC (KR)
International Classes:
C12N9/14; C07K1/16; C07K1/30; C07K1/34
Foreign References:
| US20210171572A1 | 2021-06-10 | |||
| US20160176921A1 | 2016-06-23 | |||
| US20200361985A1 | 2020-11-19 | |||
| US20210054024A1 | 2021-02-25 | |||
| US20170218012A1 | 2017-08-03 |
Attorney, Agent or Firm:
LEE, Cheo Young et al. (KR)
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