Title:
NOVEL PROTEIN HAVING METHANE OXIDATION ACTIVITY
Document Type and Number:
WIPO Patent Application WO/2022/231183
Kind Code:
A1
Abstract:
The present invention relates to a protein in which ferritin monomers, in which a methane oxidation activity domain, and an electron transfer domain comprising a flavin adenine dinucleotide (FAD)-binding domain are fused, are self-assembled, and to: a protein which can oxidize methane by using NADH in vivo as a reducing agent, thus being capable of oxidizing methane to methanol without using a separate reducing agent; a microorganism expressing same; a composition for producing methanol by using same; and a method for producing methanol.
Inventors:
LEE JEE WON (KR)
YU YEON HWA (KR)
CHOI YOO BIN (KR)
YU YEON HWA (KR)
CHOI YOO BIN (KR)
Application Number:
PCT/KR2022/005480
Publication Date:
November 03, 2022
Filing Date:
April 15, 2022
Export Citation:
Assignee:
UNIV KOREA RES & BUS FOUND (KR)
International Classes:
C12N9/02; C07K14/47; C12N15/70; C12P7/04
Foreign References:
| KR20200114723A | 2020-10-07 |
Other References:
ROSS MATTHEW O.; ROSENZWEIG AMY C.: "A tale of two methane monooxygenases", JBIC. JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY, SPRINGER, DE, vol. 22, no. 2, 22 November 2016 (2016-11-22), DE , pages 307 - 319, XP036187985, ISSN: 0949-8257, DOI: 10.1007/s00775-016-1419-y
HYUN JIN KIM, JUNE HUH, YOUNG WAN KWON, DONGHYUN PARK, YEONHWA YU, YOUNG EUN JANG, BO-RAM LEE, EUNJI JO, EUN JUNG LEE, YUNSEOK HEO: "Biological conversion of methane to methanol through genetic reassembly of native catalytic domains", NATURE CATALYSIS, vol. 2, no. 4, 1 April 2019 (2019-04-01), pages 342 - 353, XP055745544, DOI: 10.1038/s41929-019-0255-1
PARK YEO, YOO HEE, SONG MIN, LEE DONG-HEON, LEE SEUNG: "Biocatalytic Oxidations of Substrates through Soluble Methane Monooxygenase from Methylosinus sporium 5", CATALYSTS, vol. 8, no. 12, pages 582, XP055982492, DOI: 10.3390/catal8120582
YU ZHANG, BRENDAN P. ORNER: "Self-Assembly in the Ferritin Nano-Cage Protein Superfamily", INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, MOLECULAR DIVERSITY PRESERVATION INTERNATIONAL (MDPI), BASEL, CH, vol. 12, no. 12, 22 December 2011 (2011-12-22), Basel, CH , pages 5406 - 5421, XP055427570, ISSN: 1661-6596, DOI: 10.3390/ijms12085406
HYUN JIN KIM, JUNE HUH, YOUNG WAN KWON, DONGHYUN PARK, YEONHWA YU, YOUNG EUN JANG, BO-RAM LEE, EUNJI JO, EUN JUNG LEE, YUNSEOK HEO: "Biological conversion of methane to methanol through genetic reassembly of native catalytic domains", NATURE CATALYSIS, vol. 2, no. 4, 1 April 2019 (2019-04-01), pages 342 - 353, XP055745544, DOI: 10.1038/s41929-019-0255-1
PARK YEO, YOO HEE, SONG MIN, LEE DONG-HEON, LEE SEUNG: "Biocatalytic Oxidations of Substrates through Soluble Methane Monooxygenase from Methylosinus sporium 5", CATALYSTS, vol. 8, no. 12, pages 582, XP055982492, DOI: 10.3390/catal8120582
YU ZHANG, BRENDAN P. ORNER: "Self-Assembly in the Ferritin Nano-Cage Protein Superfamily", INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, MOLECULAR DIVERSITY PRESERVATION INTERNATIONAL (MDPI), BASEL, CH, vol. 12, no. 12, 22 December 2011 (2011-12-22), Basel, CH , pages 5406 - 5421, XP055427570, ISSN: 1661-6596, DOI: 10.3390/ijms12085406
Attorney, Agent or Firm:
LEECHAE INTELLECTUAL PROPERTY (KR)
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