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Title:
NOVEL PROTEIN HAVING METHANE OR BUTANE OXIDATION ACTIVITY
Document Type and Number:
WIPO Patent Application WO/2022/231184
Kind Code:
A1
Abstract:
The present invention relates to: a protein having activity for producing methanol or butanol by oxidizing methane or butanol; a microorganism expressing same; a composition for producing methanol or butanol using same; and a method for producing methanol or butanol, wherein the protein is a self-assembled protein comprising a ferritin monomer fused with an ammonia oxidase active domain having methane oxidation activity, or a butane oxidase active domain having butane oxidation activity.

Inventors:
LEE JEE WON (KR)
YU YEON HWA (KR)
CHOI YOO BIN (KR)
Application Number:
PCT/KR2022/005481
Publication Date:
November 03, 2022
Filing Date:
April 15, 2022
Export Citation:
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Assignee:
UNIV KOREA RES & BUS FOUND (KR)
International Classes:
C12N9/02; C07K14/195; C07K14/47; C12N15/70; C12P7/04; C12P7/16
Foreign References:
KR20200114723A2020-10-07
Other References:
LU YONGZE; LI XIN; CHEN YUE; WANG YONGZHEN; ZHU GUANGCAN; ZENG RAYMOND JIANXIONG: "The indispensable role of assimilation in methane driven nitrate removal", SCIENCE OF THE TOTAL ENVIRONMENT, ELSEVIER, AMSTERDAM, NL, vol. 746, 24 July 2020 (2020-07-24), AMSTERDAM, NL , XP086279410, ISSN: 0048-9697, DOI: 10.1016/j.scitotenv.2020.141089
SLUIS MIRIAM K, SAYAVEDRA-SOTO LUIS A, ARP DANIEL J: "Molecular analysis of the soluble butane monooxygenase from ' Pseudomonas butanovora '", MICROBIOLOGY, 1 January 2002 (2002-01-01), pages 3617 - 3629, XP055980401, Retrieved from the Internet [retrieved on 20221111]
SAYAVEDRA-SOTO LUIS A., HAMAMURA NATSUKO, LIU CHIH-WEN, KIMBREL JEFFREY A., CHANG JEFF H., ARP DANIEL J.: "The membrane-associated monooxygenase in the butane-oxidizing Gram-positive bacterium Nocardioides sp. strain CF8 is a novel member of the AMO/PMO family : pBMO in Nocardioides sp. strain CF8", ENVIRONMENTAL MICROBIOLOGY REPORTS, WILEY-BLACKWELL PUBLISHING, GB, vol. 3, no. 3, 1 June 2011 (2011-06-01), GB , pages 390 - 396, XP055980436, ISSN: 1758-2229, DOI: 10.1111/j.1758-2229.2010.00239.x
YU ZHANG, BRENDAN P. ORNER: "Self-Assembly in the Ferritin Nano-Cage Protein Superfamily", INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, MOLECULAR DIVERSITY PRESERVATION INTERNATIONAL (MDPI), BASEL, CH, vol. 12, no. 12, 22 December 2011 (2011-12-22), Basel, CH , pages 5406 - 5421, XP055427570, ISSN: 1661-6596, DOI: 10.3390/ijms12085406
Attorney, Agent or Firm:
LEECHAE INTELLECTUAL PROPERTY (KR)
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