Title:
NOVEL TRUNCATED SORTASE VARIANTS
Document Type and Number:
WIPO Patent Application WO/2021/185360
Kind Code:
A1
Abstract:
Provided are novel truncated sortase variants. Specifically, provided is a truncated variant of a wild type Staphylococcus aureus sortase A, wherein the truncated variant lacks N-terminal amino acids 1-59 or 2-59 of the wild type Staphylococcus aureus sortase A. Provided also is a method of expressing a sortase, comprising: (a) providing a host cell comprising a vector comprising a polynucleotide encoding the sortase, and (b) inducing the host cell to express the sortase at a temperature lower than an optimal temperature for the growth of the host cell. The truncated variant has an improved thermal stability and can be used for various polypeptide conjugating.
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Inventors:
GAO XIAOFEI (CN)
REN JUN (CN)
ZHANG QING (CN)
REN JUN (CN)
ZHANG QING (CN)
Application Number:
PCT/CN2021/081839
Publication Date:
September 23, 2021
Filing Date:
March 19, 2021
Export Citation:
Assignee:
WESTLAKE THERAPEUTICS HANGZHOU CO LTD (CN)
International Classes:
C12N9/52; C12N15/57; C12P21/00
Domestic Patent References:
| WO2014183066A2 | 2014-11-13 | |||
| WO2015042393A2 | 2015-03-26 | |||
| WO2017167712A1 | 2017-10-05 | |||
| WO2014041072A1 | 2014-03-20 |
Foreign References:
| CN109797194A | 2019-05-24 | |||
| CN110295157A | 2019-10-01 |
Other References:
WOJCIK, M ET AL.: "Sortase mutants with improved protein thermostability and enzymatic activity obtained by consensus design", PROTEIN ENGINEERING, DESIGN AND SELECTION, vol. 32, no. 12, 31 December 2019 (2019-12-31), pages 555 - 564, XP055852225, DOI: 10.1093/protein/gzaa018
Attorney, Agent or Firm:
BEIJING GLOBE-LAW LAW FIRM (CN)
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