Title:
NUCLEIC ACID APTAMER FOR MICROVESICLE
Document Type and Number:
WIPO Patent Application WO/2015/083731
Kind Code:
A1
Abstract:
Provided is a nucleic acid aptamer exhibiting binding capacity with respect to a microvesicle. Also provided are: a microvesicle detection/isolation method using said aptamer exhibiting binding capacity with respect to the microvesicle; and a drug delivery system. This nucleic acid aptamer includes: 5'-X1-Ms-X2-3' (Ms represents a base sequence which is at least 80% identical to YRCGGNGGWGTWGGGGR (SED IQ NO: 209)), and X1 and X2 bind complementarily to form stems of 0-50 base pairs); or 5'-X3-(N)m-Mk-(N)n-X4-3' (Mk represents a base sequence which is at least 80% identical to either RRRDDRNDRGRKW (SED ID NO: 208) or RVDDGGGHTCTAC (SEQ ID NO: 211), m and n each represent an integer from 0 to 20, and X3 and X4 bind complementarily to form stems of 0-50 base pairs).
Inventors:
MIYAGISHI MAKOTO (JP)
MURAKAMI KAZUYOSHI (JP)
YAMASAKI KAZUHIKO (JP)
IKEMOTO MITSUSHI (JP)
MURAKAMI KAZUYOSHI (JP)
YAMASAKI KAZUHIKO (JP)
IKEMOTO MITSUSHI (JP)
Application Number:
PCT/JP2014/081954
Publication Date:
June 11, 2015
Filing Date:
December 03, 2014
Export Citation:
Assignee:
NAT INST OF ADVANCED IND SCIEN (JP)
International Classes:
C12N15/115; A61K9/127; A61K47/34; A61K47/48; C12N5/07; C12N15/09; C12Q1/68
Domestic Patent References:
| WO2012002541A1 | 2012-01-05 | |||
| WO2014068408A2 | 2014-05-08 | |||
| WO2014100434A1 | 2014-06-26 |
Foreign References:
| JP2007525177A | 2007-09-06 | |||
| JP2012508577A | 2012-04-12 | |||
| JP2011510663A | 2011-04-07 |
Other References:
JANAS, T. ET AL.: "The Selection of Aptamers Specific for Membrane Molecular Targets", CELLULAR & MOLECULAR BIOLOGY LETTERS, vol. 16, 2010, pages 25 - 39, XP035994664, DOI: doi:10.2478/s11658-010-0023-3
Attorney, Agent or Firm:
OKUYAMA, Shoichi et al. (JP)
Okuyama In addition, it is 1. (JP)
Okuyama In addition, it is 1. (JP)
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