Title:
NUCLEIC ACID COLLECTION METHOD, NUCLEIC-ACID-BINDING CARRIER, AND NUCLEIC ACID COLLECTION KIT
Document Type and Number:
WIPO Patent Application WO/2019/035335
Kind Code:
A1
Abstract:
Provided are: a method for collecting nucleic acid at a high rate of collection by means of a simple operation, even when the nucleic acid is long; a nucleic-acid-binding carrier that is suitable for use in the collection method; and a nucleic acid collection kit. A nucleic acid collection method that involves: forming composites by making nucleic acid bind to porous silica particles that have a D50 of 6–50 μm in a volume-based particle size distribution, a pore volume of 0.3–5 cm3/g as found by the BJH method, and an average pore diameter of 60–500 Å as found by the BJH method or porous silica particles that have a D50 of 6–50 μm in a volume-based particle size distribution, a pore volume of 0.6–5 cm3/g as found by mercury porosimetry, and an average pore diameter of 200–5,000 Å as found by mercury porosimetry; liquating the nucleic acid from the composites; and collecting the nucleic acid.
Inventors:
SAKAI TOMOHIRO (JP)
HARA FUTOSHI (JP)
KOTANI TETSUYA (JP)
YAMASAKI SHINSUKE (JP)
HARA FUTOSHI (JP)
KOTANI TETSUYA (JP)
YAMASAKI SHINSUKE (JP)
Application Number:
PCT/JP2018/028130
Publication Date:
February 21, 2019
Filing Date:
July 26, 2018
Export Citation:
Assignee:
AGC INC (JP)
International Classes:
C12N15/10; C12M1/00; C12M1/26; B01D15/00; B01J20/10; B01J20/28; B01J20/34; C01B33/12
Foreign References:
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| JP2014526973A | 2014-10-09 | |||
| JP2009092592A | 2009-04-30 | |||
| JPH11313670A | 1999-11-16 | |||
| JP2003507049A | 2003-02-25 | |||
| JP2000300262A | 2000-10-31 | |||
| JP2007286226A | 2007-11-01 | |||
| JP2008220260A | 2008-09-25 | |||
| JP2008072987A | 2008-04-03 | |||
| US20090221809A1 | 2009-09-03 | |||
| JP2017003527A | 2017-01-05 | |||
| US20050287583A1 | 2005-12-29 | |||
| US20150275269A1 | 2015-10-01 | |||
| JPH09505724A | 1997-06-10 |
Other References:
ZHANG, L. ET AL.: "Preparation of porous magnetic silica microspheres and their application in genomic deoxyribonucleic acids extraction", CHINESE JOURNAL OF ANALYTICAL CHEMISTRY, vol. 34, no. 7, 2006, pages 923 - 926, XP022857778, DOI: doi:10.1016/S1872-2040(06)60043-1
GUNAL, G. ET AL.: "Human genomic DNA isolation from whole blood using a simple microfluidic system with silica- and polymer-based stationary phases", MATERIALS SCIENCE AND ENGINEERING C, vol. 74, 10 January 2017 (2017-01-10), pages 10 - 20
ZHANG, Z. ET AL.: "Synthesis of novel porous magnetic silica microspheres as adsorbents for isolation of genomic DNA", BIOTECHNOLOGY PROGRESS, vol. 22, no. 2, 2006, pages 514 - 518, XP002440337, DOI: doi:10.1021/bp050400w
SAKASHITA, SHIZUKA: "Fundamental Powder Technology for Engineer Related to Colour Material Field : Chap. 1 Physical Property of Particle and Powder", J. JPN. SOC. COLOUR MATER., vol. 78, 2005, pages 168 - 184
GUNAL, G. ET AL.: "Human genomic DNA isolation from whole blood using a simple microfluidic system with silica- and polymer-based stationary phases", MATERIALS SCIENCE AND ENGINEERING C, vol. 74, 10 January 2017 (2017-01-10), pages 10 - 20
ZHANG, Z. ET AL.: "Synthesis of novel porous magnetic silica microspheres as adsorbents for isolation of genomic DNA", BIOTECHNOLOGY PROGRESS, vol. 22, no. 2, 2006, pages 514 - 518, XP002440337, DOI: doi:10.1021/bp050400w
SAKASHITA, SHIZUKA: "Fundamental Powder Technology for Engineer Related to Colour Material Field : Chap. 1 Physical Property of Particle and Powder", J. JPN. SOC. COLOUR MATER., vol. 78, 2005, pages 168 - 184
Attorney, Agent or Firm:
SENMYO Kenji et al. (JP)
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