NUCLEIC ACID EXTRACTION METHOD USING SOLID SUBJECT

Title:

NUCLEIC ACID EXTRACTION METHOD USING SOLID SUBJECT

Document Type and Number:

WIPO Patent Application WO/2017/200249

Kind Code:

A1

Abstract:

The present invention relates to a nucleic acid extraction method using a solid subject. By forming a complex between a nucleic acid sample and an imidoester compound within the subject, it is possible to separate nucleic acids in a large amount and in high purity more simply and at a lower cost than conventional nucleic acid extraction methods. In particular, a thin film device, used for extracting a nucleic acid, has improved hydrophilic properties compared to conventional silicon substrates, and thus can extract the nucleic acid more efficiently.

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Inventors:

SHIN YONG (KR)

Application Number:

PCT/KR2017/005019

Publication Date:

November 23, 2017

Filing Date:

May 15, 2017

Export Citation:

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Assignee:

UNIV ULSAN FOUND IND COOP (KR)
ASAN FOUND (KR)

International Classes:

C12Q1/68; B01L3/00; C12N15/10

Foreign References:

KR20150096444A	2015-08-24
US20070196833A1	2007-08-23

Other References:

SHIN, Y. ET AL.: "Dimethyl Adipimidate/Thin Film Sample processing (DTS); A Simple, Low-cost, and Versatile Nucleic Acid Extraction Assay for Downstream Analysis", SCIENTIFIC REPORTS, vol. 5, no. 14127, 2015, pages 1 - 11, XP055556165
SHIN, Y. ET AL.: "Solid Phase Nucleic Acid extraction Technique in a Microfluidic Chip Using a Novel Non-chaotropic Agent: Dimethyl Adipimidate", LAB ON A CHIP, vol. 14, no. 2, 2014, pages 359 - 368, XP055261226
SUTER, J. D. ET AL.: "Label-free DNA Methylation Analysis using Opto-fluidicring Resonators", BIOSENSORS AND BIOELECTRONICS, vol. 26, no. 3, 2010, pages 1016 - 1020, XP027393251

Attorney, Agent or Firm:

TAEBAEK INTELLECTUAL PROPERTY LAW FIRM (KR)

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