Title:
OLFACTORY RECEPTOR EXPRESSING METHOD AND RESPONSE MEASUREMENT METHOD
Document Type and Number:
WIPO Patent Application WO/2022/270481
Kind Code:
A1
Abstract:
The present invention provides a technique that enables expression of an olfactory receptor and enables the olfactory receptor to function. This olfactory receptor polypeptide expression method comprises causing a cell to express an olfactory receptor polypeptide having an amino acid sequence obtained by altering at least one amino acid residue that is in an amino acid sequence of a target olfactory receptor but that is different in a consensus amino acid sequence, to an amino acid residue of the consensus amino acid sequence at a position corresponding thereto. The consensus amino acid sequence is an amino acid sequence derived through alignment of the amino acid sequence of the target olfactory receptor and an amino acid sequence of a specific olfactory receptor.
Inventors:
YOSHIKAWA KEIICHI (JP)
Application Number:
PCT/JP2022/024613
Publication Date:
December 29, 2022
Filing Date:
June 20, 2022
Export Citation:
Assignee:
KAO CORP (JP)
International Classes:
C12N15/12; C07K14/705; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N15/09; C12N15/63; C12Q1/02
Domestic Patent References:
| WO2020006108A2 | 2020-01-02 | |||
| WO2020006108A2 | 2020-01-02 | |||
| WO2016194788A1 | 2016-12-08 |
Foreign References:
| JP2018074944A | 2018-05-17 | |||
| JP2020010629A | 2020-01-23 | |||
| JP2016224039A | 2016-12-28 | |||
| JP6122181B2 | 2017-04-26 | |||
| JP2020510436A | 2020-04-09 | |||
| JP2020513564A | 2020-05-14 |
Other References:
IKEGAMI KENTARO, DE MARCH CLAIRE A., NAGAI MAIRA H., GHOSH SOUMADWIP, DO MATTHEW, SHARMA RUCHIRA, BRUGUERA ELISE S., LU YUEYANG ER: "Structural instability and divergence from conserved residues underlie intracellular retention of mammalian odorant receptors", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 117, no. 6, 11 February 2020 (2020-02-11), pages 2957 - 2967, XP055894313, ISSN: 0027-8424, DOI: 10.1073/pnas.1915520117
SCHMIEDEBERG, K. SHIROKOVA, E. WEBER, H.P. SCHILLING, B. MEYERHOF, W. KRAUTWURST, D.: "Structural determinants of odorant recognition by the human olfactory receptors OR1A1 and OR1A2", JOURNAL OF STRUCTURAL BIOLOGY, vol. 159, no. 3, 1 September 2007 (2007-09-01), United States , pages 400 - 412, XP022229942, ISSN: 1047-8477, DOI: 10.1016/j.jsb.2007.04.013
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SAITOU H ET AL., CELL, vol. 119, 2004, pages 679 - 91
IKEGAMI K ET AL., PNAS, vol. 117, 2020, pages 2957 - 2967
POREBSKI TB ET AL., PROTEIN ENGINEERING, DESIGN & SELECTION, vol. 29, 2016, pages 245 - 251
NAT. NEUROSCI., vol. 5, 2004, pages 263 - 278
SCIENCE, vol. 227, 1985, pages 1435 - 41
THOMPSON, J.D. ET AL., NUCLEIC ACIDS RES., vol. 22, 1994, pages 4673 - 4680
BLOCK E ET AL., NAT. PROD. REP., vol. 34, no. 5, 2017, pages 529 - 557
ZHUANG ET AL., J BIOL CHEM., vol. 282, no. 20, 18 May 2007 (2007-05-18), pages 15284 - 93
ERIKSSON ET AL., FLAVOUR, vol. 1, 2012, pages 22
SATO-AKUHARA ET AL., J NEUROSCI., vol. 36, no. 16, 2016, pages 4482 - 4491
SCHMIEDEBERG, K. SHIROKOVA, E. WEBER, H.P. SCHILLING, B. MEYERHOF, W. KRAUTWURST, D.: "Structural determinants of odorant recognition by the human olfactory receptors OR1A1 and OR1A2", JOURNAL OF STRUCTURAL BIOLOGY, vol. 159, no. 3, 1 September 2007 (2007-09-01), United States , pages 400 - 412, XP022229942, ISSN: 1047-8477, DOI: 10.1016/j.jsb.2007.04.013
NIIMURA Y ET AL., GENOME RESEARCH, vol. 24, 2014, pages 1485 - 1496
TRIMMER C ET AL., PNAS, vol. 116, 2019, pages 9475 - 9480
SAITOU H ET AL., CELL, vol. 119, 2004, pages 679 - 91
IKEGAMI K ET AL., PNAS, vol. 117, 2020, pages 2957 - 2967
POREBSKI TB ET AL., PROTEIN ENGINEERING, DESIGN & SELECTION, vol. 29, 2016, pages 245 - 251
NAT. NEUROSCI., vol. 5, 2004, pages 263 - 278
SCIENCE, vol. 227, 1985, pages 1435 - 41
THOMPSON, J.D. ET AL., NUCLEIC ACIDS RES., vol. 22, 1994, pages 4673 - 4680
BLOCK E ET AL., NAT. PROD. REP., vol. 34, no. 5, 2017, pages 529 - 557
ZHUANG ET AL., J BIOL CHEM., vol. 282, no. 20, 18 May 2007 (2007-05-18), pages 15284 - 93
ERIKSSON ET AL., FLAVOUR, vol. 1, 2012, pages 22
SATO-AKUHARA ET AL., J NEUROSCI., vol. 36, no. 16, 2016, pages 4482 - 4491
Attorney, Agent or Firm:
THE PATENT CORPORATE BODY ARUGA PATENT OFFICE (JP)
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