OLIGOPEPTIDE SEQUENCE SPECIFICALLY BONDING TO PHENYLBORONIC ACID GROUP

WIPO Patent Application WO/2012/105471

A1

The purpose of the present invention is to provide, as a method for immobilizing a protein molecule, a protein immobilizing method and means that enables control of the orientation of a molecule to be immobilized, that does not require complicated steps, that is capable of stable immobilization, and that uses a chemical group for immobilization that does not impact the activity or function of the protein. Specifically, the present invention relates to a method for immobilizing a molecule that comprises a step for adhering a labeled peptide sequence containing a hydroxyl group-containing amino acid to a molecule to form a labeled molecule and a step for bringing a molecule having a phenylboronic acid group into contact with the labeled molecule to capture the labeled molecule with the molecule having a phenylboronic acid group.

KONNO TOMOHIRO (JP)
ISHIHARA KAZUHIKO (JP)
NISHIDA HIROKAZU (JP)

PCT/JP2012/051933

August 09, 2012

January 30, 2012

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HITACHI LTD (JP)
UNIV TOKYO (JP)
KONNO TOMOHIRO (JP)
ISHIHARA KAZUHIKO (JP)
NISHIDA HIROKAZU (JP)

G01N33/53; C07K17/00; G01N33/543

WO2009112498A1

2009-09-17

JPH08507857A	1996-08-20
JP2004531390A	2004-10-14
JP2002255995A	2002-09-11

NISHIDA HIROKAZU ET AL.: "Novel immobilization method for protein molecule with phenylboronic acid group by using peptide tag", BIOPHYSICS, vol. 51, no. SUPP.1, 15 August 2011 (2011-08-15), pages S142 - 143

HIRAKI Yusuke et al. (JP)
Yusuke Hiraki (JP)

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