Title:
PCR PRIMER, PCR AMPLIFICATION METHOD AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2020/124472
Kind Code:
A1
Abstract:
Disclosed is a PCR primer, a PCR amplification method and a use thereof. A forward primer and a reverse primer of the primer comprise an identical fixed sequence, and the forward primer and the reverse primer each comprise a specific primer sequence that is different from the other. The primer of the present invention employs the fixed sequence to cause primer dimer to inhibit cervical-loop structures formed by itself, such that the cervical-loop structures can be used as a template for subsequent PCR amplification, greatly reducing the primer dimer during the PCR process.
Inventors:
YANG LIN (CN)
YANG GUIFANG (CN)
ZHANG YANYAN (CN)
CHEN FANG (CN)
JIANG HUI (CN)
YANG GUIFANG (CN)
ZHANG YANYAN (CN)
CHEN FANG (CN)
JIANG HUI (CN)
Application Number:
PCT/CN2018/122275
Publication Date:
June 25, 2020
Filing Date:
December 20, 2018
Export Citation:
Assignee:
MGI TECH CO LTD (CN)
International Classes:
C12Q1/686; C40B50/06
Foreign References:
| CN1597976A | 2005-03-23 | |||
| CN105256048A | 2016-01-20 | |||
| CN106282353A | 2017-01-04 | |||
| CN107119331A | 2017-09-01 | |||
| CN107447258A | 2017-12-08 | |||
| CN106319639A | 2017-01-11 |
Other References:
BROWNIE, J. ET AL.: "The Elimination of Primer-dimer Accumulation in PCR", NUCLEIC ACIDS RESEARCH, vol. 25, no. 16, 31 December 1997 (1997-12-31), XP002930982, ISSN: 0305-1048, DOI: 10.1093/nar/25.16.3235
Attorney, Agent or Firm:
DHC IP ATTORNEYS (CN)
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