Title:
PHOTOFUNCTIONAL NUCLEIC ACID, METHOD OF PRODUCING THE SAME AND METHOD OF DETERMINING METHYLATION
Document Type and Number:
WIPO Patent Application WO/2006/137190
Kind Code:
A1
Abstract:
It is intended to provide a photofunctional nucleic acid by which the methylation state of cytosine can be clearly determined even in the case of a DNA strand having a consecutive guanine sequence. In a photofunctional nucleic acid of one mode, one base in an oligonucleotide having a sequence complementary to the base sequence of a p53 gene containing the cytosine region, the methylation state of which is to be determined, is substituted by a naphthoquinone compound. This naphthoquinone compound is located at such a position as facing to the cytosine to be determined when the photofunctional nucleic acid is hybridized with the DNA having the base sequence of the p53 gene. The photofunctional nucleic acid is hybridized with the DNA having the base sequence of the p53 gene and then photo irradiated. Thus, the DNA is cleaved exclusively in the case where the cytosine facing to the naphthoquinone compound has been methylated. By analyzing a DNA fragment with the use of polyacrylamide gel electrophoresis, therefor, it can be determined whether the cytosine contained in the DNA has been methylated or not.
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Inventors:
NISHIMOTO SEI-ICHI
TANABE KAZUHITO
YAMADA HISATSUGU
TANABE KAZUHITO
YAMADA HISATSUGU
Application Number:
PCT/JP2006/302332
Publication Date:
December 28, 2006
Filing Date:
February 10, 2006
Export Citation:
Assignee:
UNIV KYOTO (JP)
NISHIMOTO SEI-ICHI
TANABE KAZUHITO
YAMADA HISATSUGU
NISHIMOTO SEI-ICHI
TANABE KAZUHITO
YAMADA HISATSUGU
International Classes:
C12N15/09; C12Q1/68
Other References:
YAMADA H.: "2-Methyl-1,4-naphthoquinone Donyu Hikari Kinosei Kakusan: Hikari Sanka Hanno o Oyo shita 5-methylcytosine Hen'i DNA Enki Bui no Kenshutsu", KYOTO UNIVERSITY FACULTY OF ENGINEERING DEPARTMENT OF ENERGY AND HYDROCARBON CHEMISTRY SHUSHI RONBUN YOSHISHU (HEISEI 16 NENDO), 11 February 2005 (2005-02-11), pages 65 - 68, XP003004697
YAMADA H. ET AL.: "Naphthoquinone Bui o Motsu Hikari Kinosei Kakusan no Gosei to Bussei: 5-methylcytosine Hen'i Bui ni Okeru Hikari Sankateki DNA Setsudan. (Photo-induced oxidative......naphthoquinone chromophores)", CSJ: THE CHEMICAL SOCIETY OF JAPAN DAI 85 SHUNKI NENKAI 2005 NEN KOEN YOKOSHU II, 11 March 2005 (2005-03-11), pages 1366 (LECTURE NO. 2G6-07), XP003004698
ZHENG Q. ET AL.: "Introduction of structural diversity into oligonucleotides containing 6-thioguanine via on-column conjugation", TETRAHEDRON, vol. 59, no. 11, 2003, pages 1925 - 1932, XP004413133
CONNOLLY B.A.: "Chemical synthesis of oligonucleotides containing a free sulphydryl group and subsequent attachment of thiol specific probes", NUCLEIC ACIDS RESEARCH, vol. 13, no. 12, 1985, pages 4485 - 4502, XP000877196
YAMADA H. ET AL.: "Naphthoquinone Bui o Motsu Hikari Kinosei Kakusan no Gosei to Bussei: 5-methylcytosine Hen'i Bui ni Okeru Hikari Sankateki DNA Setsudan. (Photo-induced oxidative......naphthoquinone chromophores)", CSJ: THE CHEMICAL SOCIETY OF JAPAN DAI 85 SHUNKI NENKAI 2005 NEN KOEN YOKOSHU II, 11 March 2005 (2005-03-11), pages 1366 (LECTURE NO. 2G6-07), XP003004698
ZHENG Q. ET AL.: "Introduction of structural diversity into oligonucleotides containing 6-thioguanine via on-column conjugation", TETRAHEDRON, vol. 59, no. 11, 2003, pages 1925 - 1932, XP004413133
CONNOLLY B.A.: "Chemical synthesis of oligonucleotides containing a free sulphydryl group and subsequent attachment of thiol specific probes", NUCLEIC ACIDS RESEARCH, vol. 13, no. 12, 1985, pages 4485 - 4502, XP000877196
Attorney, Agent or Firm:
HARAKENZO WORLD PATENT & TRADEMARK (Tenjinbashi 2-chome Kita, Kita-k, Osaka-shi Osaka 41, JP)
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