Title:
PLATELET MEMBRANE SELF-ASSEMBLY NANOBUBBLE, AND PREPARATION METHOD AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2020/199788
Kind Code:
A1
Abstract:
A platelet membrane self-assembly nanobubble, and a preparation method and application thereof. The preparation process comprises the following steps of: (1) performing repeated freezing and thawing on platelets, and obtaining a purified platelet membrane vesicle suspension after washing; and performing homogenization by means of a water bath ultrasonic effect; and (2) implementing self-assembly and reorganization of platelet membrane fragments on a gas-liquid interface after the homogenized platelet membrane vesicle suspension is subjected to ultrasonic cavitation fragmentation or gas-liquid mixing repeated extrusion, so as to construct and form a platelet membrane-coated nanobubble. The preparation method of the platelet membrane nanobubble is simple; and the nanobubble which has a nanometer size and retains the natural properties of the platelet membrane has high biocompatibility and vascular injury targeting, and can be used for ultrasound imaging diagnosis of a vascular injury part, thereby solving the problem of difficult targeted ultrasound imaging diagnosis of early small micro-lesions of cardio-cerebrovascular diseases.
Inventors:
YANG FANG (CN)
LI MINGXI (CN)
GU NING (CN)
LI MINGXI (CN)
GU NING (CN)
Application Number:
PCT/CN2020/076443
Publication Date:
October 08, 2020
Filing Date:
February 24, 2020
Export Citation:
Assignee:
UNIV SOUTHEAST (CN)
International Classes:
A61K49/22
Domestic Patent References:
| WO2017211906A1 | 2017-12-14 |
Foreign References:
| CN109364263A | 2019-02-22 | |||
| CN110090310A | 2019-08-06 | |||
| CN109100504A | 2018-12-28 |
Other References:
LI, MINGXI ET AL.: "Platelet Bio-Nanobubbles as Microvascular Recanalization Nanoformulation for Acute Ischemic Stroke Lesion Theranostics.", THERANOSTICS., vol. 8, no. 18, 9 September 2018 (2018-09-09), XP55740957, DOI: 20200513151630X
Attorney, Agent or Firm:
NANJING SUGAO PATENT AND TRADEMARK FIRM ( ORDINARY PARTNERSHIP ) (CN)
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