Title:
POLYPEPTIDE CONTAINING DNA-BINDING DOMAIN
Document Type and Number:
WIPO Patent Application WO/2015/019672
Kind Code:
A1
Abstract:
Provided are: an artificial nuclease that contains a DNA-binding domain and a functional domain, said domains being bonded together via a polypeptide consisting of 35-55 amino acids, wherein amino acids at two positions in DNA-binding modules present in the DNA-binding domain show a repetition pattern that is different in each four DNA-binding modules; a vector for expressing the artificial nuclease; a vector library for constructing the vector; and a vector set for constructing the vector library.
Inventors:
YAMAMOTO TAKASHI (JP)
SAKUMA TETSUSHI (JP)
OCHIAI HIROSHI (JP)
MATSUURA SHINYA (JP)
MIYAMOTO TATSUO (JP)
SAKUMA TETSUSHI (JP)
OCHIAI HIROSHI (JP)
MATSUURA SHINYA (JP)
MIYAMOTO TATSUO (JP)
Application Number:
PCT/JP2014/062518
Publication Date:
February 12, 2015
Filing Date:
May 09, 2014
Export Citation:
Assignee:
UNIV HIROSHIMA (JP)
International Classes:
C12N15/09; C07K14/195; C07K19/00; C12N9/22; C40B40/08
Foreign References:
| JP2013529083A | 2011-09-20 | |||
| JP2013094148A | 2013-05-20 |
Other References:
TETSUSHI SAKUMA ET AL.: "Advanced methods for the construction, evaluation and application of TALENs", MEETING OF THE 45TH ANNUAL MEETING OF THE JAPANESE SOCIETY OF DEVELOPMENTAL BIOLOGISTS & THE 64TH ANNUAL MEETING OF THE JAPAN SOCIETY FOR CELL BIOLOGY PROGTAM & ABSTRACTS, 27 April 2012 (2012-04-27), pages 303
TETSUSHI SAKUMA ET AL.: "Platinum Gate TALEN: Establishment of highly-active TALEN construction system", DAI 46 KAI JAPANESE SOCIETY OF DEVELOPMENTAL BIOLOGISTS YOKOSHU, 15 May 2013 (2013-05-15)
SATOSHI OTA ET AL.: "Efficient identification of TALEN-mediated genome modifications using heteroduplex mobility assays", GENES TO CELLS, vol. 18, no. 6, June 2013 (2013-06-01), pages 450 - 458, XP008165148, DOI: doi:10.1111/gtc.12050
JEFFREY C MILLER ET AL.: "A TALE nuclease architecture for efficient genome editing", NATURE BIOTECHNOLOGY, vol. 29, 2011, pages 143 - 148
THOMAS GAJ ET AL.: "ZFN, TALEN, and CRISPR/Cas- based methods for genome engineering", TRENDS IN BIOTECHNOLOGY, vol. 31, no. 7, July 2013 (2013-07-01), pages 397 - 405, XP028571313, DOI: doi:10.1016/j.tibtech.2013.04.004
TOMAS CERMAK ET AL.: "Efficient design and assembly of custom TALEN and other TAL effector -based constructs for DNA targeting", NUCLEIC ACIDS RESEARCH, vol. 39, no. 12, 2011, pages E82, XP002659530, DOI: doi:10.1093/nar/gkr218
JENS BOCH ET AL.: "Xanthomonas AvrBs3 Family-Type III Effectors: Discovery and Function", ANNUAL REVIEW OF PHYTOPATHOLOGY, vol. 48, 2010, pages 419 - 436, XP055070452, DOI: doi:10.1146/annurev-phyto-080508-081936
TETSUSHI SAKUMA ET AL.: "Repeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity", SCIENTIFIC REPORTS, vol. 3, 29 November 2013 (2013-11-29)
TETSUSHI SAKUMA ET AL.: "Platinum Gate TALEN: Establishment of highly-active TALEN construction system", DAI 46 KAI JAPANESE SOCIETY OF DEVELOPMENTAL BIOLOGISTS YOKOSHU, 15 May 2013 (2013-05-15)
SATOSHI OTA ET AL.: "Efficient identification of TALEN-mediated genome modifications using heteroduplex mobility assays", GENES TO CELLS, vol. 18, no. 6, June 2013 (2013-06-01), pages 450 - 458, XP008165148, DOI: doi:10.1111/gtc.12050
JEFFREY C MILLER ET AL.: "A TALE nuclease architecture for efficient genome editing", NATURE BIOTECHNOLOGY, vol. 29, 2011, pages 143 - 148
THOMAS GAJ ET AL.: "ZFN, TALEN, and CRISPR/Cas- based methods for genome engineering", TRENDS IN BIOTECHNOLOGY, vol. 31, no. 7, July 2013 (2013-07-01), pages 397 - 405, XP028571313, DOI: doi:10.1016/j.tibtech.2013.04.004
TOMAS CERMAK ET AL.: "Efficient design and assembly of custom TALEN and other TAL effector -based constructs for DNA targeting", NUCLEIC ACIDS RESEARCH, vol. 39, no. 12, 2011, pages E82, XP002659530, DOI: doi:10.1093/nar/gkr218
JENS BOCH ET AL.: "Xanthomonas AvrBs3 Family-Type III Effectors: Discovery and Function", ANNUAL REVIEW OF PHYTOPATHOLOGY, vol. 48, 2010, pages 419 - 436, XP055070452, DOI: doi:10.1146/annurev-phyto-080508-081936
TETSUSHI SAKUMA ET AL.: "Repeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity", SCIENTIFIC REPORTS, vol. 3, 29 November 2013 (2013-11-29)
Attorney, Agent or Firm:
SAMEJIMA, Mutsumi et al. (JP)
Mutsumi Sameshima (JP)
Mutsumi Sameshima (JP)
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