Title:
THE POLYPEPTIDE FRAGMENTS OF HEPATITIS E VIRUS, THE VACCINE COMPOSITION COMPRISING SAID FRAGMENTS AND THE DIAGNOSTIC KITS
Document Type and Number:
WIPO Patent Application WO/2002/040681
Kind Code:
A1
Abstract:
The invention relates to n-met of polypeptide comprising the amino acids of ORF-2 of the hepatitis E virus shown by the sequences of SEQ ID NO:1 or fragments thereof, wherein n is integer of 2-180. The invention also relates to the chimeric polypeptide of said fragments fused to conservative fragment of influenza virus hemagglutinin, and to covalent binding of said fragments with epitope polypeptide of ORF3 of hepatitis E virus or its fragments of immungenic activity, and to an expression vector comprising DNA molecules coding for above polypeptide, an expression host comprising expression vector. The invention also relates to vaccine composition and a kit of comprising above said polypeptide of HEV for diagnosing infection of HEV, and to diagnostic kits comprising IgG, IgM and total antibody, and its uses for preventing, diagnosing and/or treating infection of HEV.
Inventors:
XIA NINGSHAO (CN)
ZHANG JUN (CN)
LI SHAOWEI (CN)
GE SHENGXIANG (CN)
GU YING (CN)
HE ZHIGIANG (CN)
ZHANG JUN (CN)
LI SHAOWEI (CN)
GE SHENGXIANG (CN)
GU YING (CN)
HE ZHIGIANG (CN)
Application Number:
PCT/CN2001/001469
Publication Date:
May 23, 2002
Filing Date:
September 30, 2001
Export Citation:
Assignee:
YANG SHENG TANG CO LTD (CN)
XIA NINGSHAO (CN)
ZHANG JUN (CN)
LI SHAOWEI (CN)
GE SHENGXIANG (CN)
GU YING (CN)
HE ZHIGIANG (CN)
XIA NINGSHAO (CN)
ZHANG JUN (CN)
LI SHAOWEI (CN)
GE SHENGXIANG (CN)
GU YING (CN)
HE ZHIGIANG (CN)
International Classes:
A61K38/00; G01N33/53; A61K39/29; A61K39/295; A61P1/16; A61P31/12; C07K14/08; C07K14/705; C07K19/00; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N15/09; C12N15/51; G01N33/543; G01N33/566; G01N33/576; G01N33/577; (IPC1-7): C12N15/51
Domestic Patent References:
| WO1996012807A2 | 1996-05-02 | |||
| WO1995017501A1 | 1995-06-29 | |||
| WO1996012807A2 | 1996-05-02 | |||
| WO1995017501A1 | 1995-06-29 |
Foreign References:
| US6054567A | 2000-04-25 | |||
| US6054567A | 2000-04-25 |
Other References:
VISVANATHAN, INDIAN J. MED. RES., vol. 45, 1957, pages 1 - 30
WONG ET AL., LANCET, vol. 2, 1980, pages 882 - 885
MYINT ET AL., AM J TROP MED HYG., vol. 34, 1985, pages 1183 - 1189
BELABBES ET AL., J MED VIROL., vol. 16, 1985, pages 257 - 263
HAU ET AL., AM J TROP MED HYG., vol. 60, 1999, pages 277 - 280
TSEGA ET AL., CLIN. INFEC DIS., vol. 14, 1992, pages 961 - 965
DILAWARI ET AL., INDIAN J GASTROENTEROL., vol. 13, 1994, pages 44 - 48
HUSSAINI ET AL., J VIRAL HEPAT., vol. 4, 1997, pages 51 - 54
LI ET AL., J. VIROL., 1997, pages 7207 - 7213
ZHANG ET AL., CLIN DIAGN LAB IMMUNOL., vol. 4, 1997, pages 423 - 8
MCATEE ET AL., PROTEIN EXPR. PURIF., vol. 8, 1996, pages 262 - 270
MCEWEN J. ET AL., VACCINE, vol. 10, no. 6, 1992, pages 405 - 11
JOSEPH SAMBROOK; DAVID W.RUSSELL: "Molecular Cloning: a Laboratory Manual", COLD SPRING HARBOR LABORATORY PRESS
"Short Protocols in Molecular Biology", 1995, JOHN WILEY & SONS,INC.
AYE,T.T.; UCHIDA, NUCLEIC ACIDS RESEARCH, vol. 20, no. 13, 1992, pages 3512
See also references of EP 1331271A4
WONG ET AL., LANCET, vol. 2, 1980, pages 882 - 885
MYINT ET AL., AM J TROP MED HYG., vol. 34, 1985, pages 1183 - 1189
BELABBES ET AL., J MED VIROL., vol. 16, 1985, pages 257 - 263
HAU ET AL., AM J TROP MED HYG., vol. 60, 1999, pages 277 - 280
TSEGA ET AL., CLIN. INFEC DIS., vol. 14, 1992, pages 961 - 965
DILAWARI ET AL., INDIAN J GASTROENTEROL., vol. 13, 1994, pages 44 - 48
HUSSAINI ET AL., J VIRAL HEPAT., vol. 4, 1997, pages 51 - 54
LI ET AL., J. VIROL., 1997, pages 7207 - 7213
ZHANG ET AL., CLIN DIAGN LAB IMMUNOL., vol. 4, 1997, pages 423 - 8
MCATEE ET AL., PROTEIN EXPR. PURIF., vol. 8, 1996, pages 262 - 270
MCEWEN J. ET AL., VACCINE, vol. 10, no. 6, 1992, pages 405 - 11
JOSEPH SAMBROOK; DAVID W.RUSSELL: "Molecular Cloning: a Laboratory Manual", COLD SPRING HARBOR LABORATORY PRESS
"Short Protocols in Molecular Biology", 1995, JOHN WILEY & SONS,INC.
AYE,T.T.; UCHIDA, NUCLEIC ACIDS RESEARCH, vol. 20, no. 13, 1992, pages 3512
See also references of EP 1331271A4
Attorney, Agent or Firm:
CCPIT PATENT AND TRADEMARK LAW OFFICE (2 Fuchengmenwai Street, Beijing 7, CN)
Download PDF: