Title:
PREPARATION METHOD FOR NON-ESTER-TYPE TEA POLYPHENOL RICH IN EGC
Document Type and Number:
WIPO Patent Application WO/2022/156615
Kind Code:
A1
Abstract:
The present invention relates to a preparation method for a non-ester-type tea polyphenol rich in EGC, the method comprising: subjecting crushed and wall-broken tea leaves to enzymatic hydrolysis by using endogenous hydrolase at the content thereof in the tea leaves themselves, leaching the tea leaves after enzymatic hydrolysis, filtering and concentrating the leaching liquid, then refining and purifying same by means of mobile extraction-water washing, and concentrating and drying the purified product to obtain a non-ester-type tea polyphenol product rich in EGC, the tea polyphenol content being 90-99.5%, the EGC content being greater than 80%, and the content of ester-type catechin EGCG+ECG being 10% or less. By means of the preparation method for the non-ester-type tea polyphenol rich in EGC of the present invention, no exogenous hydrolase is added, thereby avoiding exogenous hydrolase contamination in the product; and in addition, the EGC content is high, the production process is simplified, and the product yield is high, which are beneficial for industrial production.
Inventors:
REN XUEYIN (CN)
LIU JIANHONG (CN)
CAO HOUJIAN (CN)
PU PINGNAN (CN)
LIU DEHE (CN)
LIU JIANHONG (CN)
CAO HOUJIAN (CN)
PU PINGNAN (CN)
LIU DEHE (CN)
Application Number:
PCT/CN2022/072230
Publication Date:
July 28, 2022
Filing Date:
January 17, 2022
Export Citation:
Assignee:
JIANGSU DEHE BIOTECHNOLOGY CO LTD (CN)
International Classes:
C07G99/00; C07D311/62
Foreign References:
| CN112574161A | 2021-03-30 | |||
| CN102443614A | 2012-05-09 | |||
| CN103242282A | 2013-08-14 | |||
| CN109077290A | 2018-12-25 | |||
| CN107232326A | 2017-10-10 | |||
| US6113965A | 2000-09-05 | |||
| CN1626668A | 2005-06-15 | |||
| CN104292200A | 2015-01-21 | |||
| JP2011168579A | 2011-09-01 |
Other References:
WANG XIANBO, CUI HONGCHUN, HUANG HAITAO: "Optimized Extraction Conditions for EGC From Tea by Response Surface Methodology", JOURNAL OF TEA, vol. 37, no. 4, 10 December 2011 (2011-12-10), pages 221 - 224, XP055954517, ISSN: 0577-8921
YANG WEILI, YU LIJUN, WANG RUOZHONG, TANG HAO, WEN HAITAO, DENG KENEY: "The Variantional Regular of Endogenous Enzyme Activity During the Processing of Oolong Tea", FOOD AND MACHINERY, vol. 20, no. 6, 1 December 2004 (2004-12-01), pages 5 - 7, XP055954523
YANG WEILI, YU LIJUN, WANG RUOZHONG, TANG HAO, WEN HAITAO, DENG KENEY: "The Variantional Regular of Endogenous Enzyme Activity During the Processing of Oolong Tea", FOOD AND MACHINERY, vol. 20, no. 6, 1 December 2004 (2004-12-01), pages 5 - 7, XP055954523
Attorney, Agent or Firm:
WUXI YIHAI INTELLECTUAL PROPERTY AGENCY (GENERAL PARTNERSHIP) (CN)
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