Title:
PREPARATION METHOD AND USE OF PHYSICAL STIMULATION TO CONTROL LYSIS AND INTRACELLULAR SUBSTANCE RELEASE OF BACTERIA CAPABLE OF PRODUCING BACTERIAL CELLULOSE
Document Type and Number:
WIPO Patent Application WO/2023/197093
Kind Code:
A1
Abstract:
Provided are preparation method and use of physical stimulation to control lysis and intracellular substance release of bacteria capable of producing bacterial cellulose. Provided is a plasmid for physically controlling bacterial lysis, wherein the plasmid has a physically activated promoter and a lysis protein-encoding gene amplified under the regulation of the physically activated promoter, a ribosome binding site (RBS) is provided in the upstream of the lysis protein-encoding gene, and the RBS has a sequence set forth in SEQ ID NO: 3 or a sequence having one, two, three, or four base mutations in SEQ ID NO: 3. The problems of self-clearing and intracellular substance release of host bacteria are solved by constructing a lysis system in the host bacteria, which has controllable conditions and high accuracy. The precise regulation of lysis of the engineered bacteria is realized by regulating the RBS site of the lysis protein.
Inventors:
GAO YANMEI (CN)
JIN FAN (CN)
JIN FAN (CN)
Application Number:
PCT/CN2022/086016
Publication Date:
October 19, 2023
Filing Date:
April 11, 2022
Export Citation:
Assignee:
SHENZHEN INST OF ADV TECH CAS (CN)
International Classes:
C12N9/90; C12N1/21; C12P19/04; C12R1/01; C12R1/02; C12R1/05
Foreign References:
| CN101302486A | 2008-11-12 | |||
| CN108624545A | 2018-10-09 | |||
| US20210147855A1 | 2021-05-20 | |||
| CN101041844A | 2007-09-26 | |||
| CN106244613A | 2016-12-21 | |||
| CN112011555A | 2020-12-01 | |||
| CN105969786A | 2016-09-28 |
Other References:
KENNETH J. B. ET AL.: "Role of Premature Translational Termination in the Regulation of Expression of the ΦX174 Lysis Gene", JOURNAL OF MOLECULAR BIOLOGY, vol. 198, 31 December 1987 (1987-12-31), XP024019685, ISSN: 0022-2836, DOI: 10.1016/0022-2836(87)90203-8
Attorney, Agent or Firm:
BEIJING CHENGHUI LAW FIRM (CN)
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