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Title:
PREPARATION FOR PERIODONTAL TISSUES, AND KIT INCLUDING SAME
Document Type and Number:
WIPO Patent Application WO/2022/220179
Kind Code:
A1
Abstract:
[Problem] To provide a novel preparation for periodontal tissues, which enables a treatment to be performed by a simple operation, can achieve high periodontal tissue regeneration performance, and can achieve a high regeneration effect from an initial stage of the treatment. [Solution] The present invention relates to a preparation for periodontal tissues, which comprises a culture supernatant and hyaluronic acid. In particular, in the preparation for periodontal tissues according to the present invention, the culture supernatant can be produced by a method comprising a first culture step for culturing cells using a first culture medium, a second culture step for culturing the cells using, as a culture medium, a second culture medium that is different from the first culture medium subsequent to the first culture step, and a step for producing a culture supernatant containing the second culture medium subsequent to the second culture step, the second culture medium can contain calcium ions and a buffer, and the buffer can be selected from Good's buffers and can be particularly HEPES.

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Inventors:
SAKAI MARI (JP)
Application Number:
PCT/JP2022/017134
Publication Date:
October 20, 2022
Filing Date:
April 06, 2022
Export Citation:
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Assignee:
SAKAI MARI (JP)
International Classes:
A61K35/16; A61K35/12; A61P1/02; A61P43/00; C12N5/071
Domestic Patent References:
WO2012117793A12012-09-07
WO2011118795A12011-09-29
WO2019216438A12019-11-14
WO2020027336A12020-02-06
Foreign References:
JP2014230685A2014-12-11
JP7057006B12022-04-19
Other References:
KAWAI TAKAMASA, KATAGIRI WATARU, OSUGI MASASHI, SUGIMURA YUKIKO, HIBI HIDEHARU, UEDA MINORU: "Secretomes from bone marrow–derived mesenchymal stromal cells enhance periodontal tissue regeneration", CYTOTHERAPY, vol. 17, no. 4, 1 April 2015 (2015-04-01), GB , pages 369 - 381, XP055897605, ISSN: 1465-3249, DOI: 10.1016/j.jcyt.2014.11.009
Attorney, Agent or Firm:
SHIOKAWA, Kazuya et al. (JP)
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