Title:
PREPARATION FOR PROMOTING WOUND HEALING AND PREPARATION METHOD AND USAGE METHOD THEREOF
Document Type and Number:
WIPO Patent Application WO/2016/037388
Kind Code:
A1
Abstract:
Disclosed is a preparation for promoting wound healing, comprising a cell culture medium. The cell culture medium is formed by cultivating transfected endothelial precursor cells, and the transfected endothelial precursor cells are formed by transfecting let-7g microRNAs into endothelial precursor cells. The method for preparing the preparation for promoting wound healing comprises a separation step, a transfecting step and a cultivation step. The separation step being separating mononuclear cells from human whole blood, and inoculating the mononuclear cells on the human-fibronectin-coated plate to obtain the endothelial precursor cells. The transfecting step being transfecting let-7g microRNAs into endothelial precursor cells to obtain the transfected endothelial precursor cells. The cultivation step being cultivating the transfected endothelial precursor cells for a pre-determined time to obtain the preparation for promoting wound healing.
Inventors:
HONG TING ZHEN (CN)
LAN CHUAN QING (CN)
LU JIA QI (CN)
LIU CHENG RUI (CN)
LAN CHUAN QING (CN)
LU JIA QI (CN)
LIU CHENG RUI (CN)
Application Number:
PCT/CN2014/087374
Publication Date:
March 17, 2016
Filing Date:
September 25, 2014
Export Citation:
Assignee:
HONG TING ZHEN (CN)
International Classes:
A61K31/713; A61K31/7088
Foreign References:
| CN102573856A | 2012-07-11 | |||
| TWI421084B | 2014-01-01 |
Other References:
LIAO, Y.C. ET AL.: "Let-7g Improves Multiple Endothelial Functions Through Targeting Transforming Growth Factor-Beta and SIRT-1 Signaling", JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY, vol. 63, no. 16, 29 April 2014 (2014-04-29), pages 1685 - 1694, XP028640936, DOI: doi:10.1016/j.jacc.2013.09.069
TAM, J.C.W. ET AL.: "Endothelial Progenitor Cells, a Unipotent Stem Cell , Involved in Neovascularization of Wound Healing in Diabetic Foot Ulcer", EWMA JOURNAL, vol. 12, no. 2, 31 December 2012 (2012-12-31), pages 15 - 20
LIU, Z.J. ET AL.: "Hyperoxia, Endothelial Progenitor Cell Mobilization, and Diabetic Wound Healing", ANTIOXIDANTS & REDOX SIGNALING, vol. 10, no. 11, 11 November 2008 (2008-11-11), pages 1869 - 1882
WANG, XIAORONG;: "The Role and Mechanisms of MicroRNA Let-7f in Regulating Endothelial Progenitor Cell Function in Type 1 Diabetes", MEDICINE & PUBLIC HEALTH, CHINA DOCTORAL DISSERTATIONS FULL-TEXT DATABASE, 15 November 2011 (2011-11-15), pages E065 - 5
ROUSH, S. ET AL.: "The Let-7 Family of MicroRNAs", TRENDS IN CELL BIOLOGY, vol. 18, no. 10, 4 April 2008 (2008-04-04), pages 505 - 516, XP025468977, DOI: doi:10.1016/j.tcb.2008.07.007
TAM, J.C.W. ET AL.: "Endothelial Progenitor Cells, a Unipotent Stem Cell , Involved in Neovascularization of Wound Healing in Diabetic Foot Ulcer", EWMA JOURNAL, vol. 12, no. 2, 31 December 2012 (2012-12-31), pages 15 - 20
LIU, Z.J. ET AL.: "Hyperoxia, Endothelial Progenitor Cell Mobilization, and Diabetic Wound Healing", ANTIOXIDANTS & REDOX SIGNALING, vol. 10, no. 11, 11 November 2008 (2008-11-11), pages 1869 - 1882
WANG, XIAORONG;: "The Role and Mechanisms of MicroRNA Let-7f in Regulating Endothelial Progenitor Cell Function in Type 1 Diabetes", MEDICINE & PUBLIC HEALTH, CHINA DOCTORAL DISSERTATIONS FULL-TEXT DATABASE, 15 November 2011 (2011-11-15), pages E065 - 5
ROUSH, S. ET AL.: "The Let-7 Family of MicroRNAs", TRENDS IN CELL BIOLOGY, vol. 18, no. 10, 4 April 2008 (2008-04-04), pages 505 - 516, XP025468977, DOI: doi:10.1016/j.tcb.2008.07.007
Attorney, Agent or Firm:
SHANGHAI ZHI XIN PATENT AGENT LTD. (CN)
上海智信专利代理有限公司 (CN)
上海智信专利代理有限公司 (CN)
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