Title:
PRIMER SET AND METHOD FOR DETECTING TARGET NUCLEIC ACID USING SAME
Document Type and Number:
WIPO Patent Application WO/2021/132596
Kind Code:
A1
Abstract:
One or more embodiments of the present invention solve the problem that the reaction efficiency is lowered in a nucleic acid amplification reaction using a primer, said primer having a polynucleotide tag added thereto, to prepare a target nucleic acid amplification product which is detectable on a solid phase support. One or more embodiments of the present invention pertain to a primer set comprising: a first primer containing a first polynucleotide, which contains, at the 3'-end thereof, polynucleotide A hybridizable with a complementary strand of partial polynucleotide A' at the 5'-end of a target nucleic acid, and first polynucleotide tag which is a polynucleotide independent from nucleic acid amplification reaction; a second primer containing a second polynucleotide which contains, at the 3'-end thereof, polynucleotide B hybridizable with partial polynucleotide B' at the 3'-end of the target nucleic acid; and a third primer containing a third polynucleotide, which hybridizes with the complementary strand of the target nucleic acid competitively with polynucleotide A of the first primer, but containing no polynucleotide which is independent from the nucleic acid amplification reaction.
Inventors:
MIYAMOTO SHIGEHIKO (JP)
Application Number:
PCT/JP2020/048826
Publication Date:
July 01, 2021
Filing Date:
December 25, 2020
Export Citation:
Assignee:
KANEKA CORP (JP)
International Classes:
C12N15/09; C12Q1/6844; C12Q1/6876
Domestic Patent References:
| WO2013162026A1 | 2013-10-31 | |||
| WO2013038534A1 | 2013-03-21 | |||
| WO2017043114A1 | 2017-03-16 | |||
| WO2018038232A1 | 2018-03-01 |
Foreign References:
| JP2008525037A | 2008-07-17 | |||
| JP2016073312A | 2016-05-12 | |||
| JP5688702B2 | 2015-03-25 | |||
| JP2019239045A | 2019-12-27 |
Other References:
FRONTIERS IN MICROBIOLOGY, vol. 8
GREENSAMBROOK: "Molecular Cloning", 2012, COLD SPRING HARBOR LABORATORY PRESS
ALTSCHUL ET AL., NUC. ACIDS RES., vol. 25, 1977, pages 3389 - 3402
ALTSCHUL ET AL., J. MOL. BIOL., vol. 215, 1990, pages 403 - 410
ANAL. BIOCHEM., vol. 364, 2007, pages 78 - 85
See also references of EP 4083202A4
GREENSAMBROOK: "Molecular Cloning", 2012, COLD SPRING HARBOR LABORATORY PRESS
ALTSCHUL ET AL., NUC. ACIDS RES., vol. 25, 1977, pages 3389 - 3402
ALTSCHUL ET AL., J. MOL. BIOL., vol. 215, 1990, pages 403 - 410
ANAL. BIOCHEM., vol. 364, 2007, pages 78 - 85
See also references of EP 4083202A4
Attorney, Agent or Firm:
HIRAKI & ASSOCIATES (JP)
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