Title:
PRIMERS, PROBE AND KIT FOR DETECTING NDRG4 GENE METHYLATION FOR EARLY DIAGNOSIS OF INTESTINAL CANCER
Document Type and Number:
WIPO Patent Application WO/2017/124854
Kind Code:
A1
Abstract:
The present invention discloses primers, a probe and a kit for detecting an NDRG4 gene methylation for early diagnosis of an intestinal cancer. The primers are NDRG4-1FP and NDRG4-1RP, the nucleotide sequences thereof being set forth in SEQ ID NOs. 1 and 2 respectively. The probe is NDRG4-2Pb, the nucleotide sequence thereof being set forth in SEQ ID NO. 3. The kit comprises the primers and the probe. The primers and the probe are used to establish a set of systems and procedures for detecting the NDRG4 gene methylation and to prepare the kit. The primers, the probe and the kit are highly sensitive and specific for detecting the NDRG4 gene methylation, can accurately and specifically diagnose an early intestinal cancer, and provide an easily operable and fast detection method. The present invention offers great significance and application prospect for early diagnosis and screening of an intestinal cancer.
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Inventors:
ZOU HONGZHI (CN)
WU SHAN (CN)
NIU ZHITONG (CN)
ZHAO RONGSONG (CN)
YU HAO (CN)
WU SHAN (CN)
NIU ZHITONG (CN)
ZHAO RONGSONG (CN)
YU HAO (CN)
Application Number:
PCT/CN2016/109629
Publication Date:
July 27, 2017
Filing Date:
December 13, 2016
Export Citation:
Assignee:
CREATIVE BIOSCIENCES (GUANGZHOU) CO LTD (CN)
International Classes:
C12Q1/68; C12N15/11
Domestic Patent References:
| WO2013142545A1 | 2013-09-26 |
Foreign References:
| CN105543378A | 2016-05-04 | |||
| CN104450680A | 2015-03-25 | |||
| US20150275314A1 | 2015-10-01 |
Other References:
ZOU, H. Z. ET AL.: "Quantification of methylated markers with a multiplex methylation-specific technology", CLINICAL CHEMISTRY, vol. 58, no. 2, 1 February 2012 (2012-02-01), pages 375 - 383, XP055105588, DOI: 10.1373/clinchem.2011.171264
KÄNEL, THOMAS VON ET AL.: "DNA methylation analysis", SWISS MEDICAL WEEKLY, vol. 143, 28 May 2013 (2013-05-28), pages 1 - 16, XP055598970, DOI: 10.4414/smw.2013.13799
AHLQUIST, D. A. ET AL.: "The Stool DNA Test is More Accurate than the Plasma Septin 9 Test in Detecting Colorectal Neoplasia", CLIN GASTROENTEROL HEPATOL., vol. 10, no. 3, 31 March 2012 (2012-03-31), pages 272 - 277.e1, XP055167696, DOI: 10.1016/j.cgh.2011.10.008
MELOTTE, V. ET AL.: "N-Myc Downstream-Regulated Gene 4 ( NDRG4 ): A Candidate Tumor Suppressor Gene and Potential Biomarker for Colorectal Cancer", JNCI., vol. 101, no. 13, 1 July 2009 (2009-07-01), pages 916 - 927, XP055207624, DOI: 10.1093/jnci/djp131
KÄNEL, THOMAS VON ET AL.: "DNA methylation analysis", SWISS MEDICAL WEEKLY, vol. 143, 28 May 2013 (2013-05-28), pages 1 - 16, XP055598970, DOI: 10.4414/smw.2013.13799
AHLQUIST, D. A. ET AL.: "The Stool DNA Test is More Accurate than the Plasma Septin 9 Test in Detecting Colorectal Neoplasia", CLIN GASTROENTEROL HEPATOL., vol. 10, no. 3, 31 March 2012 (2012-03-31), pages 272 - 277.e1, XP055167696, DOI: 10.1016/j.cgh.2011.10.008
MELOTTE, V. ET AL.: "N-Myc Downstream-Regulated Gene 4 ( NDRG4 ): A Candidate Tumor Suppressor Gene and Potential Biomarker for Colorectal Cancer", JNCI., vol. 101, no. 13, 1 July 2009 (2009-07-01), pages 916 - 927, XP055207624, DOI: 10.1093/jnci/djp131
Attorney, Agent or Firm:
UNITALEN ATTORNEYS AT LAW (CN)
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