Title:
PROBE REAGENT AND FISH USING PROBE REAGENT
Document Type and Number:
WIPO Patent Application WO/2015/141856
Kind Code:
A1
Abstract:
The present invention provides: a probe reagent that uses a nucleic acid having a base number that is shorter than a BAC probe to minimize nonspecific adsorption and that makes it possible to stably obtain a fluorescence signal in FISH; and FISH that uses the probe reagent. The probe reagent is for in situ hybridization in which a fluorescent body-integrated nanoparticle that is obtained by integrating a fluorescent body is bound with a nucleic acid molecule of a predetermined nucleic acid sequence. As a result, nonspecific adsorption is prevented when performing FISH in which a specific area of a chromosome is stained and it is possible to improve the detection precision with respect to a specific gene, it is possible to obtain a fluorescence signal that is sufficiently intense for observation using a confocal microscope, and it becomes possible to simultaneously detect bright spots that are positioned at different depths in a tissue section from the same point of view even when observing with a fluorescence microscope when fluorescent body nanoparticles are used. In addition, it becomes possible to maintain a tissue section slide on which FISH has been performed in a state in which it is possible to detect bright spots for longer than in the prior art.
Inventors:
ISODA TAKESHI (JP)
SEKIGUCHI MITSURU (JP)
HATANAKA YUTAKA (JP)
KURIHARA YOSHIKAZU (JP)
SEKIGUCHI MITSURU (JP)
HATANAKA YUTAKA (JP)
KURIHARA YOSHIKAZU (JP)
Application Number:
PCT/JP2015/058612
Publication Date:
September 24, 2015
Filing Date:
March 20, 2015
Export Citation:
Assignee:
KONICA MINOLTA INC (JP)
International Classes:
C12Q1/68; C12N15/09
Domestic Patent References:
| WO2012029342A1 | 2012-03-08 |
Foreign References:
| JP2013057037A | 2013-03-28 | |||
| JP2009507492A | 2009-02-26 |
Other References:
JIANG ZHENGRAN. ET AL.: "Detecting Genomic Aberrations by Fluorescence In Situ Hybridization with Quantum Dots-Labeled Probes", J NANOSCIENCE NANOTECHNOLOGY, vol. 7, no. 12, 2007, pages 4254 - 4259
WANG XIAOJUAN. ET AL.: "The Emerging Use of Quantum Dots in Analysis", ANAL LETT, vol. 40, no. 7-9, 2007, pages 1497 - 1520, XP055225519
PENG JUN. ET AL.: "Detection of Epstein-Barr Virus Infection in Gastric Carcinomas Using Quantum Dot-Based Fluorescence In-Situ Hybridization", J NANOSCIENCE NANOTECHNOLOGY, vol. 11, no. 11, 2011, pages 9725 - 9730, XP055225520, DOI: doi:10.1166/jnn.2011.5319
WU SHENG-MEI. ET AL.: "Direct fluorescence in situ hybridization (FISH) in Escherichia coli with a target-specific quantum dot-based molecular beacon", BIOSENSORS BIOELECTRON, vol. 26, no. 2, 2010, pages 491 - 496, XP027320341
ALGAR W. RUSS. ET AL.: "Beyond labels: A review of the application of quantum dots as integrated components of assays, bioprobes, and biosensors utilizing optical transduction", ANAL CHIM ACTA, vol. 673, no. 1, 2010, pages 1 - 25, XP027112281
See also references of EP 3121291A4
WANG XIAOJUAN. ET AL.: "The Emerging Use of Quantum Dots in Analysis", ANAL LETT, vol. 40, no. 7-9, 2007, pages 1497 - 1520, XP055225519
PENG JUN. ET AL.: "Detection of Epstein-Barr Virus Infection in Gastric Carcinomas Using Quantum Dot-Based Fluorescence In-Situ Hybridization", J NANOSCIENCE NANOTECHNOLOGY, vol. 11, no. 11, 2011, pages 9725 - 9730, XP055225520, DOI: doi:10.1166/jnn.2011.5319
WU SHENG-MEI. ET AL.: "Direct fluorescence in situ hybridization (FISH) in Escherichia coli with a target-specific quantum dot-based molecular beacon", BIOSENSORS BIOELECTRON, vol. 26, no. 2, 2010, pages 491 - 496, XP027320341
ALGAR W. RUSS. ET AL.: "Beyond labels: A review of the application of quantum dots as integrated components of assays, bioprobes, and biosensors utilizing optical transduction", ANAL CHIM ACTA, vol. 673, no. 1, 2010, pages 1 - 25, XP027112281
See also references of EP 3121291A4
Attorney, Agent or Firm:
SSINPAT PATENT FIRM (JP)
Patent business corporation SSINPAT (JP)
Patent business corporation SSINPAT (JP)
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