Title:
PROCESS FOR PRODUCING PRENYL ALCOHOL
Document Type and Number:
WIPO Patent Application WO/2002/053745
Kind Code:
A1
Abstract:
A process for producing prenyl alcohol characterized by comprising constructing a recombinant by transferring an expression recombinant DNA or a DNA fragment for genome integration, which contains hydroxymethylglutaryl-CoA reductase gene, isepentenyl diphosphate $g(D)-isomerase gene or farnesyl diphosphate synthase gene or a variant gene thereof together with a transcriptional promoter and a transcriptional terminator, into a host, culturing the resultant recombinant and then collecting prenyl alcohol from the culture medium thus obtained.
Inventors:
OHTO CHIKARA (JP)
OBATA SHUSEI (JP)
OBATA SHUSEI (JP)
Application Number:
PCT/JP2001/011213
Publication Date:
July 11, 2002
Filing Date:
December 20, 2001
Export Citation:
Assignee:
TOYOTA MOTOR CO LTD (JP)
OHTO CHIKARA (JP)
OBATA SHUSEI (JP)
OHTO CHIKARA (JP)
OBATA SHUSEI (JP)
International Classes:
C12N15/09; C12N1/19; C12N1/21; C12N9/04; C12N9/10; C12N9/90; C12N15/52; C12P7/04; C12P7/16; C12R1/19; C12R1/865; (IPC1-7): C12N15/52; C12N1/19; C12N1/21; C12P7/04
Domestic Patent References:
| WO2000001650A1 | 2000-01-13 |
Other References:
POLAKOWSKI T. ET AL.: "Overexpression of a cytosolic hydroxymethyl-CoA-reductase leads to squalene accumulation in yeast; recombinant enzyme expression in saccharomyces cerevisiae; metabolic engineering for use in ergosterol production", APPL. MICROBIOL. BIOTECHNOL., vol. 49, no. 1, 1998, pages 66 - 71, XP002909495
PLOCHOCKA D. ET AL.: "The role of ERG20 gene (encoding yeast farnesyl diphosphate synthase) mutation in long dolichol formation. Molecular modeling of FPP synthase", BIOCHIMIE, vol. 82, no. 8, August 2000 (2000-08-01), pages 733 - 738, XP002909496
BLANCHARD L. ET AL.: "Characterization of a lysine-to-glutamic acid mutation in a conservative sequence of farnesyl diphosphate synthase from saccharomyces cerevisiae", GENE, vol. 125, no. 2, 1993, pages 185 - 189, XP002909497
CHAMBON C. ET AL.: "Isolation and properties of yeast mutants affected in farnesyl diphosphate synthetase", CURR. GENET., vol. 18, 1990, pages 41 - 46, XP002909498
See also references of EP 1354954A4
PLOCHOCKA D. ET AL.: "The role of ERG20 gene (encoding yeast farnesyl diphosphate synthase) mutation in long dolichol formation. Molecular modeling of FPP synthase", BIOCHIMIE, vol. 82, no. 8, August 2000 (2000-08-01), pages 733 - 738, XP002909496
BLANCHARD L. ET AL.: "Characterization of a lysine-to-glutamic acid mutation in a conservative sequence of farnesyl diphosphate synthase from saccharomyces cerevisiae", GENE, vol. 125, no. 2, 1993, pages 185 - 189, XP002909497
CHAMBON C. ET AL.: "Isolation and properties of yeast mutants affected in farnesyl diphosphate synthetase", CURR. GENET., vol. 18, 1990, pages 41 - 46, XP002909498
See also references of EP 1354954A4
Attorney, Agent or Firm:
Hiraki, Yusuke (17-1 Toranomon 1-chome Minato-ku, Tokyo, JP)
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