PRODUCTION METHOD FOR CULTURE SUPERNATANT PREPARATION

WIPO Patent Application WO/2020/027336

A1

[Problem] The purpose of the present invention is to provide a culture supernatant preparation that excels in biocompatibility and contains a specific gene or protein in abundance. [Solution] A production method for a culture supernatant preparation includes: a first culturing step in which a first medium is used to culture cells to a confluent state; a second culturing step, which is performed after the first culturing step, in which a second medium that is different from the first medium is used to culture the cells; and a culture supernatant preparation obtaining step, which is performed after the second culturing step, in which a culture supernatant preparation including the second medium is obtained. The second medium includes calcium ions and a buffering agent.

INOUE HAJIME (JP)
FUJITA CHIHARU (JP)

PCT/JP2019/030608

February 06, 2020

August 02, 2019

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LABORATORY OF CELL APPLIED TECH CO (JP)

A23L33/10; A61K35/28; A61K8/98; A61K9/08; A61K9/10; A61K35/36; A61K47/02; C12N5/077; C12N5/0775

WO1987003284A1	1987-06-04
WO2017110425A1	2017-06-29

JP2017214321A	2017-12-07
JP2011510662A	2011-04-07

SIGMA-ALDRICH, DMEM, 24 September 2019 (2019-09-24), Retrieved from the Internet
SIGMA-ALDRICH, MEM, 25 September 2019 (2019-09-25), Retrieved from the Internet
NTAI A ET AL.: "Trehalose to cryopreserve human pluripotent stem cells", STEM CELL RES., vol. 31, 1 August 2018 (2018-08-01), pages 102 - 112, XP055684608, ISSN: 1873-5061, DOI: 10.1016/j.scr.2018.07.021

HIROSE Takayuki (JP)

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