Title:
PRODUCTION METHOD FOR PROTEIN
Document Type and Number:
WIPO Patent Application WO/2019/139108
Kind Code:
A1
Abstract:
Provided is a production method for high production of crystal protein inside bacteria cells of Bacillus bacteria. This production method is for crystal protein or a cultured product containing same and includes: transforming Bacillus bacteria using an expression plasmid wherein a gene that codes crystal protein and a control area including a σA-dependent promoter or a σH-dependent promoter are operably linked and incorporated; and said transformed cells are cultured. The expression plasmid includes a polynucleotide that codes a replication protein comprising an amino acid sequence expressed by SEQ ID NO: 9 or a protein having at least 80% homology with same in the amino acid sequence and is involved to replication initiation.
Inventors:
LIU SHENGHAO (JP)
KAGEYAMA YASUSHI (JP)
TERAI MIKA (JP)
KAGEYAMA YASUSHI (JP)
TERAI MIKA (JP)
Application Number:
PCT/JP2019/000618
Publication Date:
July 18, 2019
Filing Date:
January 11, 2019
Export Citation:
Assignee:
KAO CORP (JP)
International Classes:
C12N15/32; C12N1/21; C12N15/75; C12P21/02
Domestic Patent References:
| WO2011049227A1 | 2011-04-28 | |||
| WO2016007355A1 | 2016-01-14 | |||
| WO2017123946A1 | 2017-07-20 |
Foreign References:
| JPS62294080A | 1987-12-21 | |||
| JPS63133999A | 1988-06-06 | |||
| JPS6317687A | 1988-01-25 | |||
| JP2017079639A | 2017-05-18 | |||
| JP4485341B2 | 2010-06-23 | |||
| JP4336082B2 | 2009-09-30 | |||
| US20050271642A1 | 2005-12-08 | |||
| JP2018070565A | 2018-05-10 | |||
| JP2018177656A | 2018-11-15 | |||
| JP5361484B2 | 2013-12-04 | |||
| JP2000210081A | 2000-08-02 | |||
| JP2011103875A | 2011-06-02 | |||
| JP4955358B2 | 2012-06-20 | |||
| JP2017079639A | 2017-05-18 | |||
| JP4485341B2 | 2010-06-23 | |||
| JP4336082B2 | 2009-09-30 | |||
| JP2017079640A | 2017-05-18 |
Other References:
YANG, C.-Y. ET AL.: "Enterotoxigenicity and cytotoxicity of Bacillus thuringiensis strains and development of a process for CrylAc production", J. AGRIC. FOOD CHEM., vol. 51, no. 1, 2003, pages 100 - 105, XP055625604, ISSN: 0021-8561, DOI: 10.1021/jf025863l
SINGH AUPADHYAY VUPADHYAY AKSINGH SMPANDA AK: "Protein recovery from inclusion bodies of Escherichia coli using mild solubilization process", MICROB CELL FACT., vol. 25, 2015, pages 14 - 41
HIROKI HIGASHIBATA: "ABC for highly expressing heterologous protein using Escherichia coli as a host", BIOTECHNOLOGY, vol. 91, 2013, pages 96 - 100
CHUMANN, WOLFGANGFERREIRA, LUIS CARLOS S.: "Production of recombinant proteins in Escherichia coli", GENET. MOL. BIOL., vol. 27, no. 3, 2004, pages 442 - 453
GOMES ARBYREGOWDA SMVEEREGOWDA BMBALAMURUGAN V: "An overview of heterologous expression host systems for the production of recombinant proteins", ADV. ANIM. VET. SCI., vol. 4, no. 7, 2016, pages 346 - 356
FERRER-MIRALLESVILLAVERDE, BACTERIAL CELL FACTORIES FOR RECOMBINANT PROTEIN PRODUCTION, 2013
FERRER-MIRALLES, VILLAVERDE: "Bacterial cell factories for", MICROBIAL CELL FACTORIES, vol. 12, 2013, pages 113, XP021168051, DOI: 10.1186/1475-2859-12-113
HERVE' A. ET AL.: "How Does Bacillus thuringiensis Produce SO Much Insecticidal Crystal Protein", J. OF BACTERIOLOGY, vol. 177, no. 21, 1995, pages 6027 - 6032
DENG CPENG QSONG FLERECLUS D: "Regulation of cry gene expression in Bacillus thuringiensis", TOXINS, vol. 6, 2014, pages 2194 - 2209, XP055368492, DOI: 10.3390/toxins6072194
YAN HU ET AL.: "Bacillus subtilis Strain Engineered for Treatment of Soil-Transmitted Helminth Diseases", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 79, no. 18, 2013, pages 5527 - 5532
DURMAZ E. ET AL.: "Intracellular and Extracellular Expression of Bacillus thuringiensis Crystal Protein Cry5B in Lactococcus lactis for Use as an Anthelminthic", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 82, no. 4, 2016, pages 1286 - 1294, XP055368442, DOI: 10.1128/AEM.02365-15
LIPMAN, DJ.PEARSON. WR., SCIENCE, vol. 227, 1985, pages 1435 - 1441
JOSEPH SAMBROOKDAVID W. RUSSELL: "Molecular Cloning-A LABORATORY MANUAL", 2001, COLD SPRING HARBOR LABORATORY PRESS
SHINE, J.DALGARNO, L., PROC. NATL. ACAD. SCI. USA., vol. 71, 1974, pages 1342 - 1346
"Revision of the Nomenclature for the Bacillus thuringiensis Pesticidal Crystal Proteins", MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, vol. 62, 7 December 2017 (2017-12-07), pages 807 - 813, Retrieved from the Internet
CAPPELLO M ET AL., PROC. NATL. ACAD. SCI. U.S.A., vol. 103, pages 15154 - 15159
HU Y ET AL., PLOS NEGL. TROP. DIS., vol. 4, pages e614
FEMS MICROBIOL LETT, vol. 266, 2007, pages 163 - 169
MHALAKSHMI A, ADVANCES MICROBIOL., vol. 2, 2012, pages 216 - 226
PROC. NATL. ACD. SCI. USA., vol. 81, 1984, pages 439 - 443
PROC. NATL. ACD. SCI. USA, vol. 83, 1986, pages 9438 - 9442
SAMBROOK, J. ET AL.: "Molecular Cloning, A Laboratory Manual", vol. 1, 1989, COLD SPRING HARBOR LABORATORY, pages: 74
NATIONAL BIO RESOURCE PROJECT (NBRP) OF THE NATIONAL INSTITUTE OF GENETICS, Retrieved from the Internet
HAKAMADA ET AL., BIOSCI. BIOTECHNOL. BIOCHEM., vol. 64, 2000, pages 2281 - 2289
HUI ET AL., BIOCHEMISTRY, vol. 11, 2012, pages 9911 - 21
BISCHOF ET AL., METHODS IN MOLECULAR BIOLOGY, vol. 331, pages 139 - 154
LEETACHEWA ET AL., BMB REPORTS, vol. 47, 2014, pages 546 - 551
See also references of EP 3739050A4
SINGH AUPADHYAY VUPADHYAY AKSINGH SMPANDA AK: "Protein recovery from inclusion bodies of Escherichia coli using mild solubilization process", MICROB CELL FACT., vol. 25, 2015, pages 14 - 41
HIROKI HIGASHIBATA: "ABC for highly expressing heterologous protein using Escherichia coli as a host", BIOTECHNOLOGY, vol. 91, 2013, pages 96 - 100
CHUMANN, WOLFGANGFERREIRA, LUIS CARLOS S.: "Production of recombinant proteins in Escherichia coli", GENET. MOL. BIOL., vol. 27, no. 3, 2004, pages 442 - 453
GOMES ARBYREGOWDA SMVEEREGOWDA BMBALAMURUGAN V: "An overview of heterologous expression host systems for the production of recombinant proteins", ADV. ANIM. VET. SCI., vol. 4, no. 7, 2016, pages 346 - 356
FERRER-MIRALLESVILLAVERDE, BACTERIAL CELL FACTORIES FOR RECOMBINANT PROTEIN PRODUCTION, 2013
FERRER-MIRALLES, VILLAVERDE: "Bacterial cell factories for", MICROBIAL CELL FACTORIES, vol. 12, 2013, pages 113, XP021168051, DOI: 10.1186/1475-2859-12-113
HERVE' A. ET AL.: "How Does Bacillus thuringiensis Produce SO Much Insecticidal Crystal Protein", J. OF BACTERIOLOGY, vol. 177, no. 21, 1995, pages 6027 - 6032
DENG CPENG QSONG FLERECLUS D: "Regulation of cry gene expression in Bacillus thuringiensis", TOXINS, vol. 6, 2014, pages 2194 - 2209, XP055368492, DOI: 10.3390/toxins6072194
YAN HU ET AL.: "Bacillus subtilis Strain Engineered for Treatment of Soil-Transmitted Helminth Diseases", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 79, no. 18, 2013, pages 5527 - 5532
DURMAZ E. ET AL.: "Intracellular and Extracellular Expression of Bacillus thuringiensis Crystal Protein Cry5B in Lactococcus lactis for Use as an Anthelminthic", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 82, no. 4, 2016, pages 1286 - 1294, XP055368442, DOI: 10.1128/AEM.02365-15
LIPMAN, DJ.PEARSON. WR., SCIENCE, vol. 227, 1985, pages 1435 - 1441
JOSEPH SAMBROOKDAVID W. RUSSELL: "Molecular Cloning-A LABORATORY MANUAL", 2001, COLD SPRING HARBOR LABORATORY PRESS
SHINE, J.DALGARNO, L., PROC. NATL. ACAD. SCI. USA., vol. 71, 1974, pages 1342 - 1346
"Revision of the Nomenclature for the Bacillus thuringiensis Pesticidal Crystal Proteins", MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, vol. 62, 7 December 2017 (2017-12-07), pages 807 - 813, Retrieved from the Internet
CAPPELLO M ET AL., PROC. NATL. ACAD. SCI. U.S.A., vol. 103, pages 15154 - 15159
HU Y ET AL., PLOS NEGL. TROP. DIS., vol. 4, pages e614
FEMS MICROBIOL LETT, vol. 266, 2007, pages 163 - 169
MHALAKSHMI A, ADVANCES MICROBIOL., vol. 2, 2012, pages 216 - 226
PROC. NATL. ACD. SCI. USA., vol. 81, 1984, pages 439 - 443
PROC. NATL. ACD. SCI. USA, vol. 83, 1986, pages 9438 - 9442
SAMBROOK, J. ET AL.: "Molecular Cloning, A Laboratory Manual", vol. 1, 1989, COLD SPRING HARBOR LABORATORY, pages: 74
NATIONAL BIO RESOURCE PROJECT (NBRP) OF THE NATIONAL INSTITUTE OF GENETICS, Retrieved from the Internet
HAKAMADA ET AL., BIOSCI. BIOTECHNOL. BIOCHEM., vol. 64, 2000, pages 2281 - 2289
HUI ET AL., BIOCHEMISTRY, vol. 11, 2012, pages 9911 - 21
BISCHOF ET AL., METHODS IN MOLECULAR BIOLOGY, vol. 331, pages 139 - 154
LEETACHEWA ET AL., BMB REPORTS, vol. 47, 2014, pages 546 - 551
See also references of EP 3739050A4
Attorney, Agent or Firm:
THE PATENT CORPORATE BODY ARUGA PATENT OFFICE (JP)
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