PROMOTER, PROMOTER CONTROL ELEMENTS, AND COMBINATIONS, AND USES THEREOF

This National Phase PCT application claims priority under 35 U.S.C. 119(e) on U.S. Provisional Application No(s). 60/583,691 and 60/583,609 both filed on June 30, 2004.

FIELD OF THE INVENTION The present invention relates to prompters and promoter control elements that are useful for modulating transcription of a desired polynucleotide. In order to modulate in vivo and in vitro transcription of a polynucleotide such promoters and promoter control elements can be included in polynucleotide constructs, expression cassettes, vectors or inserted into the chromosome or exist in the plant cell as an exogenous element. Host cells with polynucleotides comprising the promoters and promoter control elements of the present invention which have desired traits or characteristics resulting therefrom are also a part of the invention. This includes plant cells and plants regenerated therefrom.

BACKGROUND OF THE INVENTION This invention relates to the field of biotechnology and in particular to specific promoter sequences and promoter control element sequences which are useful for the transcription of polynucleotides in a host cell or transformed host organism. One of the primary goals of biotechnology is to obtain organisms such as plants, mammals, yeast and prokaryotes that have particular desired characteristics or traits. Examples of these characteristics or traits abound and in plants may include, for example, virus resistance, insect resistance, herbicide resistance, enhanced stability, enhanced biomass, enhanced yield or additional nutritional value. Recent advances in genetic, engineering have enabled researchers in the field to incorporate polynucleotide sequences into host cells to obtain the desired qualities in the organism of choice. This technology permits one or more polynucleotides from a source different than the organism of choice to be transcribed by the organism of choice. If desired, the transcription and/or translation of these new polynucleotides can be modulated in the organism to exhibit a desired characteristic or trait. Alternatively, new patterns of transcription and/or translation of polynucleotides endogenous to the organism can be produced. Both approaches can be used at the same time.

SUMMARY OF THE INVENTION The present invention is directed to isolated polynucleotide sequences that comprise promoters and promoter control elements from plants, especially Arabidopsis thaliana, Glycine max, Oryza sativa and Zea mays, and other promoters and promoter control elements that function in plants. It is an object of the present invention to provide isolated polynucleotides that are promoter sequences. These promoter sequences comprise, for example, (1) a polynucleotide having a nucleotide sequence as set forth in the Sequence Listing or a fragment thereof, (2) a polynucleotide having a nucleotide sequence having at least 80% sequence identity to a sequence as set in the Sequence Listing or a fragment thereof, and (3) a polynucleotide having a nucleotide sequence which hybridizes to a sequence as set forth in in the Sequence Listing under a condition establishing a Tm- 200C. It is another object of the present invention to provide isolated polynucleotides that are promoter control element sequences. These promoter control element sequences comprise, for example, (1) a polynucleotide having a nucleotide sequence as set forth in the Sequence Listing or a fragment thereof, (2) a polynucleotide having a nucleotide sequence having at least 80% sequence identity to a sequence as set forth in the Sequence Listing or a fragment thereof, and (3) a polynucleotide having a nucleotide sequence which hybridizes to a sequence as set forth in the Sequence Listing under a condition establishing a Tm-20°C.

Promoter or promoter control element sequences of the present invention are capable of modulating preferential transcription. In another embodiment, the present promoter control elements are capable of serving as or fulfilling the function of, for example, a core promoter, a TATA box, a polymerase binding site, an initiator site, a transcription binding site, an enhancer, an inverted repeat, a locus control region, or a scaffold/matrix attachment region. It is yet another object of the present invention to provide a polynucleotide that includes at least a first and a second promoter control element. The first promoter control element is a promoter control element sequence as discussed above and the second promoter control element is heterologous to the first control element. Moreover, the first and second control elements are operably linked. Such promoters may modulate transcript levels preferentially in a tissue or under particular conditions. In another embodiment, the present isolated polynucleotide comprises a promoter or a promoter control element as described above, wherein the promoter or promoter control element is operably linked to a polynucleotide to be transcribed. In another embodiment of the present vector, the promoter and promoter control elements of the instant invention are operably linked to a heterologous polynucleotide that is a regulatory sequence. It is another object of the present invention to provide a host cell comprising an isolated polynucleotide or vector as described above or a fragment thereof. Host cells include, for instance, bacterial, yeast, insect cells, mammalian cells and plant cells. The host cell can comprise a promoter or promoter control element exogenous to the genome. Such a promoter can modulate transcription in cis- and in trans- orientation to the polynucletide. In yet another embodiment, the present host cell is a plant cell capable of regenerating into a plant. It is yet another embodiment of the present invention to provide a plant comprising an isolated polynucleotide or vector described above. It is another object of the present invention to provide a method of modulating transcription in a sample that contains either a cell-free system of transcription or a host cell. This method comprises providing a polynucleotide or vector according to the present invention as described above and contacting the sample of the polynucleotide or vector with conditions that permit transcription. In another embodiment of the present method, the polynucleotide or vector preferentially modulates (a) constitutive transcription, (b) stress induced transcription, (c) light or shade induced transcription, (d) dark induced transcription, (e) leaf transcription, (f) root transcription, (g) stem or shoot transcription, (h) silique or seed transcription, (i) callus transcription, (J) flower transcription, (k) immature bud and inflorescence-specific transcription, or (1) senescence induced transcription (m) germination transcription.

Other and further objects of the present invention will be made clear or become apparent from the following description.

BRIEF DESCRIPTION OF THE TABLES AND FIGURES

TABLE l Table 1 consists of the Expression Reports for each promoter of the invention and provides the nucleotide sequence for each promoter as well as details for GFP expression driven by each of the nucleic acid promoter sequences as observed in transgenic plants. The results are presented as summaries of the spatial expression, which provides information as to gross and/or specific expression in various plant organs and tissues. The observed expression pattern is also presented, which gives details of expression during different generations or different developmental stages within a generation. Additional information is provided regarding the associated gene, the GenBank reference, the source organism of the promoter and the vector and marker genes used for the construct. The following symbols are used consistently throughout the Table: - Tl: First generation transfoπnant

- T2: Second generation transformant

- T3: Third generation transformant

- (L): low expression level

- (M): medium expression level

- (H): high expression level

Each row of the table begins with heading of the data to be found in the section. The

following provides a description of the data to be found in each section:

TABLE 2

Table 2 provides a partial summary of the expression for some of the constructs of the

invention.

FIGURE l

Figure 1 is a schematic representation of the vector pNewBin4-HAPl -GFP. The definitions

of the abbreviations used in the vector map are as follows:

Ori - the origin of replication used by an E. coli host

RB - sequence for the right border of the T-DNA from pMOG800

Bsβζl — restriction enzyme cleavage site used for cloning

HAP IVP 16 - coding sequence for a fusion protein of the HAPl and VP 16 activation

domains

NOS - terminator region from the nopaline synthase gene

HAPlUAS - the upstream activating sequence for HAPl 5ERGFP - the green fluorescent protein gene that has been optimized for localization to the endoplasmic reticulum OCS2 - the terminator sequence from the octopine synthase 2 gene OCS - the terminator sequence from the octopine synthase gene p28716 (a.k.a 28716 short) - promoter used to drive expression of the PAT (BAR) gene PAT (B AR) - a marker gene conferring herbicide resistance LB - sequence for the left border of the T-DNA from pMOG800 Spec - a marker gene conferring spectinomycin resistance TrfA - transcription repression factor gene RK2-OriV — origin of replication for Agrobacterium

DETAILED DESCRIPTION OF THE INVENTION 1. Definitions Chimeric: The term "chimeric" is used to describe polynucleotides or genes, as defined supra, or constructs wherein at least two of the elements of the polynucleotide or gene or construct are heterologous to each other, such as the promoter and the polynucleotide to be transcribed and/or other regulatory sequences and/or filler sequences and/or complements thereof.

Constitutive Promoter: Promoters referred to herein as "constitutive promoters" actively promote transcription under most, but not necessarily all, environmental conditions and is essentially all cells in the vegetative stage and/or flowers and essentially all states of cell differentiation. Examples of constitutive promoters include the cauliflower mosaic virus (CaMV) 35S transcript initiation region and the 1' or 2' promoter derived from T-DNA of Agrobacterium tumefaciens as well as other transcription initiation regions from various plant genes known to those of skill in the art, such as the maize ubiquitin-1 promoter.

Core Promoter: This is the minimal stretch of contiguous DNA sequence that is sufficient to direct accurate initiation of transcription by the RNA polymerase II machinery (for review see: Struhl, 1987, CeU 49: 295-297; Smale, 1994, In Transcription: Mechanisms and Regulation (eds R.C. Conaway and J.W. Conaway), pp 63-81/ Raven Press, Ltd., New York; Smale, 1997. Biochim. Biophvs. Acta 1351: 73-88; Smale et al., 1998, Cold Spring Harb. Svmp. Quant. Biol. 58: 21-31; Smale, 2001, Genes & Dev. 15: 2503-2508; Weis and Reinberg, 1992, FASEB J. 6: 3300-3309; Burke et al., 1998, Cold Spring Harb. Svmp. Quant. Biol 63: 75- 82). There are several sequence motifs, including the TATA box, initiator (Inr), TFHB recognition element (BRE) and downstream core promoter element (DPE), that are commonly found in core promoters. Not all of these elements, however, occur in all promoters. That is, there are no universal core promoter elements (Butler and Kadonaga, 2002, Genes & Dev. 16: 2583-2592).

Domain: Domains are fingerprints or signatures that can be used to characterize protein families and/or parts of proteins. Such fingerprints or signatures can comprise conserved (1) primary sequence, (2) secondary structure, and/or (3) three-dimensional conformation. A similar analysis can be applied to polynucleotides. Generally, each domain has been associated with either a conserved primary sequence or a sequence motif. Generally these conserved primary sequence motifs have been correlated with specific in vitro and/or in vivo activities. A domain can be any length, including the entirety of the polynucleotide to be transcribed. Examples of amino acid domains include, without limitation, AP2, helicase, homeobox, zinc finger, etc. Examples of nucleotide domains include, without limitation, TATA box, CAAT box, etc.

Endogenous: The term "endogenous" within the context of the current invention refers to any polynucleotide, polypeptide or protein sequence which is a natural part of a cell or organism regenerated from said cell. In the context of promoter, the term "endogenous coding region" or "endogenous cDNA" refers to the coding region that is naturally operably linked to the promoter.

Enhancer/Suppressor: An "enhancer" is a DNA regulatory element that can increase the steady state level of a transcript, usually by increasing the rate of transcription initiation. Enhancers usually exert their effect regardless of the distance, upstream or downstream location, or orientation of the enhancer relative to the start site of transcription. In contrast, a etaletal

"suppressor" is a corresponding DNA regulatory element that decreases the steady state level of a transcript, again usually by affecting the rate of transcription initiation. The essential activity of enhancer and suppressor elements is to bind a protein factor(s). Such binding can be assayed, for example, by methods described below. The binding is typically in a manner that influences the steady state level of a transcript in a cell or in an in vitro transcription extract.

Exogenous: As referred to within, "exogenous" is any polynucleotide, polypeptide or protein sequence, whether chimeric or not, that is introduced into the genome of a host cell or organism regenerated from said host cell by any means other than by a sexual cross. Examples of means by which this can be accomplished are described below and include Agrobacterium-mediated transformation (of dicots - e.g. Salomon EMBO J. 3; 141 (1984); Herrera-Estrella EMBO J. 2:987 (1983); of monocots, representative papers are those by Escudero et ah, Plant J. 10:355 (1996), Ishida et ah, Nature Biotechnology 14:745 (1996), May et ah, Bio/Technology 13:486 (1995)), biolistic methods (Armaleo et ah, Current Genetics 1/7:97 1990)), electroporation, inplanta techniques and the like. Such a plant containing the exogenous nucleic acid is referred to here as a T0 for the primary transgenic plant and T1 for the first generation transformant. The term "exogenous" as used herein is also intended to encompass inserting a naturally found element into a non-naturally found location.

Gene: The term "gene," as used in the context of the current invention, encompasses all regulatory and coding sequence contiguously associated with a single hereditary unit with a genetic function (see SCHEMATIC 1). Genes can include non-coding sequences that modulate the genetic function that include, but are not limited to, those that specify polyadenylation, transcriptional regulation, DNA conformation, chromatin conformation, extent and position of base methylation and binding sites of proteins that control all of these. Genes encoding proteins are comprised of "exons" (coding sequences), which may be interrupted by "introns" (non-coding sequences). In some instances complexes of a plurality of protein or nucleic acids or other molecules, or of any two of the above, may be required for a gene's function. On the other hand a gene's genetic function may require only RNA expression or protein production, or may only require binding of proteins and/or nucleic acids without associated expression. In certain cases, genes adjacent to one another may share sequence in such a way that one gene will overlap the other. A gene can be found within the genome of an organism, in an artificial chromosome, in a plasmid, in any other sort of vector, or as a separate isolated entity.

Heterologous sequences: "Heterologous sequences" are those that are not operatively linked or are not contiguous to each other in nature. For example, a promoter from corn is considered heterologous to an Arabidopsis coding region sequence. Also, a promoter from a gene encoding a growth factor from corn is considered heterologous to a sequence encoding the corn receptor for the growth factor. Regulatory element sequences, such as UTRs or 3 ' end termination sequences that do not originate in nature from the same gene as the coding sequence originates from, are considered heterologous to said coding sequence. Elements operatively linked in nature and contiguous to each other are not heterologous to each other.

Homologous: In the current invention, a "homologous" gene or polynucleotide or polypeptide refers to a gene or polynucleotide or polypeptide that shares sequence similarity with the gene or polynucleotide or polypeptide of interest. This similarity may be in only a fragment of the sequence and often represents a functional domain such as, examples including without limitation a DNA binding domain or a domain with tyrosine kinase activity. The functional activities of homologous polynucleotide are not necessarily the same.

Inducible Promoter: An "inducible promoter" in the context of the current invention refers to a promoter the activity of which is influenced by certain conditions such as light, temperature, chemical concentration, protein concentration, conditions in an organism, cell, or organelle, etc. A typical example of an inducible promoter, which can be utilized with the polynucleotides of the present invention, is PARSKl, the promoter from am Arabidopsis gene encoding a serine-threonine kinase ercyme which is induced by dehydration, abscissic acid and sodium chloride (Wang and Goodman, Plant J. 8:37 (1995)). Examples of environmental conditions that may affect transcription by inducible promoters include anaerobic conditions, elevated temperature, the presence or absence of a nutrient or other chemical compound and/or the presence of light.

Modulate Transcription Level: As used herein, the phrase "modulate transcription" describes the biological activity of a promoter sequence or promoter control element. Such modulation includes, without limitation, up- and down-regulation of initiation of transcription, rate of transcription and/or transcription levels.

Mutant: In the current invention "mutant" refers to a heritable change in a mutation sequence at a specific location. Mutant genes of the current invention may or may not have an associated identifiable phenotype.

Operable Linkage: An "operable linkage" is a linkage in which a promoter sequence or promoter control element is connected to a polynucleotide sequence(s) in such a way as to place transcription of the polynucleotide sequence under the influence or control of the promoter or promoter control element. Two DNA sequences (such as a polynucleotide to be transcribed and a promoter sequence linked to the 5' end of the polynucleotide to be transcribed) are said to be operably linked if induction of promoter function results in the transcription of mRNA encoded by the polynucleotide and if the nature of the linkage between the two DNA sequences does not (1) result in the introduction of a frame-shift mutation, (2) interfere with the ability of the promoter sequence to direct the expression of the protein, antisense RNA or ribozyme or (3) interfere with the ability of the DNA template to be transcribed. Thus, a promoter sequence would be operably linked to a polynucleotide sequence if the promoter was capable of effecting transcription of that polynucleotide sequence.

Optional Promoter Fragments: The phrase "optional promoter fragments" is used to refer to any sub-sequence of the promoter that is not required for driving transcription of an operationally linked coding region. These fragments comprise the 5' UTR and any exon(s) of the endogenous coding region. The optional promoter fragments may also comprise any exon(s) and the 3' or 5' UTR of the gene residing upstream of the promoter (that is, 5' to the promoter). Optional promoter fragments also include any intervening sequences that are introns or sequence that occurs between exons or an exon and the UTR.

Orthologous: "Orthologous" is a term used herein to describe a relationship between two or more polynucleotides or proteins. Two polynucleotides or proteins are "orthologous" to one another if they serve a similar function in different organisms. In general, orthologous polynucleotides or proteins will have similar catalytic functions (when they encode enzymes) or will serve similar structural functions (when they encode proteins or RNA that form part of the ultrastracture of a cell). Generally it is believed that orthologous structures share a common evolutionary origin.

Percentage of sequence identity: "Percentage of sequence identity," as used herein, is determined by comparing two optimally aligned sequences over a comparison window, where the fragment of the polynucleotide or amino acid sequence in the comparison window may comprise additions or deletions (e.g., gaps or overhangs) as compared to the reference sequence (which does not comprise additions or deletions) for optimal alignment of the two sequences. The percentage is calculated by determining the number of positions at which the identical nucleic acid base or amino acid residue occurs in both sequences to yield the number of matched positions, dividing the number of matched positions by the total number of positions in the window of comparison and multiplying the result by 100 to yield the percentage of sequence identity. Optimal alignment of sequences for comparison may be conducted by the local homology algorithm of Smith and Waterman A dd. APL. Math. 2:482 (1981), by the homology alignment algorithm of Needleman and WunschJ. MoI. Biol. 48:443 (1970), by the search for similarity method of Pearson and Lipman Proc. Natl. Acad. ScL (USA) 85: 2444 (1988), by computerized implementations of these algorithms (GAP, BESTFIT, BLAST, PASTA, and TFASTA in the Wisconsin Genetics Software Package, Genetics Computer Group (GCG), 575 Science Dr., Madison, WI), or by inspection. Given that two sequences have been identified for comparison, GAP and BESTFIT are preferably employed to determine their optimal alignment. Typically, the default values of 5.00 for gap weight and 0.30 for gap weight length are used. The term "substantial sequence identity" between polynucleotide or polypeptide sequences refers to polynucleotide or polypeptide comprising a sequence that has at least 80% sequence identity, preferably at least 85%, more preferably at least 90% and most preferably at least 95%, even more preferably, at least 96%, 97%, 98% or 99% sequence identity compared to a reference sequence using the programs. Query nucleic acid sequences were searched against subject nucleic acid sequences residing in public or proprietary databases. Such searches were done using the Washington University Basic Local Alignment Search Tool Version 1.83 (WU-B Iast2) program. The WU-Blast2 program is available on the internet from Washington University. A WU-Blast2 service for Arabidopsis can also be found on the internet. Typically the following parameters of WU-Blast2 were used: Filter options were set to "default," Output format was set to "gapped alignments," the Comparison Matrix was set to "BLOSUM62," Cutoff Score (S value) was set to "default," the Expect (E threshold) was set to "default," the Number of best alignments to show was set to "100," and the "Sort output" option was set to sort the output by "pvalue."

Plant Promoter: A "plant promoter" is a promoter capable of initiating transcription in plant cells and can modulate transcription of a polynucleotide. Such promoters need not be of plant origin. For example, promoters derived from plant viruses, such as the CaMV35S promoter or from Agrobacterium tumefaciens such as the T-DNA promoters, can be plant promoters. A typical example of a plant promoter of plant origin is the maize ubiquitin-1 (ubi- 1) promoter known to those of skill in the art.

Plant Tissue: The term "plant tissue" includes differentiated and undifferentiated tissues or plants, including but not limited to roots, stems, shoots, cotyledons, epicotyl, hypocotyl, leaves, pollen, seeds, tumor tissue and various forms of cells in culture such as single cells, protoplast, embryos, and callus tissue. The plant tissue may be in plants or in organ, tissue or cell culture.

Preferential Transcription: "Preferential transcription" is defined as transcription that occurs in a particular pattern of cell types or developmental times or in response to specific stimuli or combination thereof. Non-limitive examples of preferential transcription include: high transcript levels of a desired sequence in root tissues; detectable transcript levels of a desired sequence in certain cell types during embryogenesis; and low transcript levels of a desired sequence under drought conditions. Such preferential transcription can be determined by measuring initiation, rate, and/or levels of transcription.

Promoter: A "promoter" is a DNA sequence that directs the transcription of a polynucleotide. Typically a promoter is located in the 5' region of a polynucleotide to be transcribed, immediately upstream to the transcriptional start site of such polynucleotide. More typically, promoters are defined as the region upstream of the first exon; more typically, as a region upstream of the first of multiple transcription start sites; more typically, as the region downstream of the preceding gene and upstream of the first of multiple transcription start sites; more typically, the region downstream of the polyA signal and upstream of the first of multiple transcription start sites; even more typically, about 3,000 nucleotides upstream of the ATG of the first exon; even more typically, 2,000 nucleotides upstream of the first of multiple transcription start sites. The promoters of the invention comprise at least a core promoter as defined above. Frequently promoters are capable of directing transcription of genes located on each of the complementary DNA strands that are 3' to the promoter. Stated differently, many promoters exhibit bidirectionality and can direct transcription of a downstream gene when present in either orientation (i.e. 5' to 3' or 3' to 5' relative to the coding region of the gene). Additionally, the promoter may also include at least one control element such as an upstream element. Such elements include UARs and optionally, other DNA sequences that affect transcription of a polynucleotide such as a synthetic upstream element.

Promoter Control Element: The term "promoter control element" as used herein describes elements that influence the activity of the promoter. Promoter control elements include transcriptional regulatory sequence determinants such as, but not limited to, enhancers, scaffold/matrix attachment regions, TATA boxes, transcription start locus control regions, UARs, URRs, other transcription factor binding sites and inverted repeats. Public sequence: The term "public sequence," as used in the context of the instant application, refers to any sequence that has been deposited in a publicly accessible database prior to the filing date of the present application. This term encompasses both amino acid and nucleotide sequences. Such sequences are publicly accessible, for example, on the BLAST databases on the NCBI FTP web site (accessible via the worldwide web). The database at the NCBI FTP site uses "gi" numbers assigned by NCBI as a unique identifier for each sequence in the database, thereby providing a non-redundant database for sequence from various databases, including GenBank, EMBL, DBBJ, (DNA Database of Japan) and PDB (Brookhaven Protein Data Bank).

Regulatory Sequence: The term "regulatory sequence," as used in the current invention, refers to any nucleotide sequence that influences transcription or translation initiation and rate, or stability and/or mobility of a transcript or polypeptide product. Regulatory sequences include, but are not limited to, promoters, promoter control elements, protein binding sequences, 5' and 3' UTRs, transcriptional start sites, termination sequences, polyadenylation sequences, introns, certain sequences within amino acid coding sequences such as secretory signals, protease cleavage sites, etc.

Related Sequences: "Related sequences" refer to either a polypeptide or a nucleotide sequence that exhibits some degree of sequence similarity with a reference sequence.

Specific Promoters: In the context of the current invention, "specific promoters" refers to a subset of promoters that have a high preference for modulating transcript levels in a specific tissue or organ or cell and/or at a specific time during development of an organism. By "high preference" is meant at least 3-fold, preferably 5-fold, more preferably at least 10- fold still more preferably at least 20-fold, 50-fold or 100-fold increase in transcript levels under the specific condition over the transcription under any other reference condition considered. Typical examples of temporal and/or tissue or organ specific promoters of plant origin that can be used with the polynucleotides of the present invention, are: PTA29, a promoter which is capable of driving gene transcription specifically in tapetum and only during anther development (Koltonow et al, Plant Cell 2: 1201 (1990); RCc2 and RCc3, promoters that direct root-specific gene transcription in rice (Xu et al., Plant MoI. Biol. 27:237 (1995); and TobRB27, a root-specific promoter from tobacco (Yamamoto et al., Plant Cell 3:371 (1991)). Examples of tissue-specific promoters under developmental control include promoters that initiate transcription only in certain tissues or organs, such as root, ovule, fruit, seeds, or flowers. Other specific promoters include those from genes encoding seed storage proteins or the lipid body membrane protein, oleosin. A few root-specific promoters are noted above. See also "Preferential transcription".

Stringency: "Stringency" as used herein is a function of probe length, probe composition (G + C content) and salt concentration, organic solvent concentration and temperature of hybridization or wash conditions. Stringency is typically compared by the parameter Tm, which is the temperature at which 50% of the complementary molecules in the hybridization are hybridized, in terms of a temperature differential from Tm. High stringency conditions are those providing a condition of Tm - 50C to Tm - 1O0C. Medium or moderate stringency conditions are those providing Tm - 2O0C to Tm - 290C. Low stringency conditions are those providing a condition of Tm - 4O0C to Tm - 480C. The relationship of hybridization conditions to T1n (in 0C) is expressed in the mathematical equation

Tm = 81.5 -16.6(1Og10[Na+]) + 0.41(%G+C) - (600/N) (1)

where N is the length of the probe. This equation works well for probes 14 to 70 nucleotides in length that are identical to the target sequence. The equation below for Tm of DNA-DNA hybrids is useful for probes in the range of 50 to greater than 500 nucleotides, and for conditions that include an organic solvent (formamide).

Tm = 81.5+16.6 log {[Na+]/(l+0.7[Na+])}+ 0.41(%G+C)-500/L 0.63 (% formamide) (2)

where L is the length of the probe in the hybrid (P. Tijessen, "Hybridization with Nucleic Acid Probes" in Laboratory Techniques in Biochemistry and Molecular Biology, P.C. vand der Vliet, ed., c. 1993 by Elsevier, Amsterdam.) The Tm of equation (2) is affected by the nature of the hybrid; for DNA-RNA hybrids Tm is 10-150C higher than calculated, for RNA- RNA hybrids Tm is 20-250C higher. Because the Tm decreases about 1 0C for each 1% decrease in homology when a long probe is used (Bonner et al. (1973) J. MoI. Biol, ^i: 123), stringency conditions can be adjusted to favor detection of identical genes or related family members. Equation (2) is derived assuming equih'brium and therefore, hybridizations according to the present invention are most preferably performed under conditions of probe excess and for sufficient time to achieve equilibrium. The time required to reach equilibrium can be shortened by inclusion of a hybridization accelerator such as dextran sulfate or another high volume polymer in the hybridization buffer. Stringency can be controlled during the hybridization reaction or after hybridization has occurred by altering the salt and temperature conditions of the wash solutions used. The formulas shown above are equally valid when used to compute the stringency of a wash solution. Preferred wash solution stringencies lie within the ranges stated above; high stringency is 5-80C below Tm medium or moderate stringency is 26-290C below Tm and low stringency is 45-480C below Tm.

Substantially free of: A composition containing A is "substantially free of B when at least 85% by weight of the total A+B in the composition is A. Preferably, A comprises at least about 90% by weight of the total of A+B in the composition, more preferably at least about 95% or even 99% by weight. For example, a plant gene can be substantially free of other plant genes. Other examples include, but are not limited to, ligands substantially free of receptors (and vice versa), a growth factor substantially free of other growth factors and a transcription binding factor substantially free of nucleic acids.

Suppressor: See "Enhancer/Suppressor"

TATA to start: "TATA to start" shall mean the distance, in number of nucleotides, between the primary TATA motif and the start of transcription.

Transgenic plant: A "transgenic plant" is a plant having one or more plant cells that contain at least one exogenous polynucleotide introduced by recombinant nucleic acid methods. Translational start site: In the context of the present invention, a "translational start site" is usually an ATG or AUG in a transcript, often the first ATG or AUG. A single protein encoding transcript, however, may have multiple translational start sites.

Transcription start site: "Transcription start site" is used in the current invention to describe the point at which transcription is initiated. This point is typically located about 25 nucleotides downstream from a TFIID binding site, such as a TATA box. Transcription can initiate at one or more sites within the gene and a single polynucleotide to be transcribed may have multiple transcriptional start sites, some of which may be specific for transcription in a particular cell-type or tissue or organ. "+1" is stated relative to the transcription start site and indicates the first nucleotide in a transcript.

Upstream Activating Region (UAR): An "Upstream Activating Region" or "UAR" is a position or orientation dependent nucleic acid element that primarily directs tissue, organ, cell type, or environmental regulation of transcript level, usually by affecting the rate of transcription initiation. Corresponding DNA elements that have a transcription inhibitory effect are called herein "Upstream Repressor Regions" or "URR"s. The essential activity of these elements is to bind a protein factor. Such binding can be assayed by methods described below. The binding is typically in a manner that influences the steady state level of a transcript in a cell or in vitro transcription extract.

Untranslated region (UTR): A "UTR" is any contiguous series of nucleotide bases that is transcribed, but is not translated. A 5' UTR lies between the start site of the transcript and the translation initiation codon and includes the +1 nucleotide. A 3 ' UTR lies between the translation termination codon and the end of the transcript. UTRs can have particular functions such as increasing mRNA message stability or translation attenuation. Examples of 3' UTRs include, but are not limited to polyadenylation signals and transcription termination sequences. Variant: The term "variant" is used herein to denote a polypeptide or protein or polynucleotide molecule that differs from others of its kind in some way. For example, polypeptide and protein variants can consist of changes in amino acid sequence and/or charge and/or post-translational modifications (such as glycosylation, etc). Likewise, polynucleotide variants can consist of changes that add or delete a specific UTR or exon sequence. It will be understood that there may be sequence variations within sequence or fragments used or disclosed in this application. Preferably, variants will be such that the sequences have at least 80%, preferably at least 90%, 95, 97, 98, or 99% sequence identity. Variants preferably measure the primary biological function of the native polypeptide or protein or polynucleotide.

2. Introduction

The polynucleotides of the invention comprise promoters and promoter control elements that are capable of modulating transcription. Such promoters and promoter control elements can be used in combination with native or heterologous promoter fragments, control elements or other regulatory sequences to modulate transcription and/or translation. Specifically, promoters and control elements of the invention can be used to modulate transcription of a desired polynucleotide, which includes without limitation: (a) antisense; (b) ribozymes; (c) coding sequences; or (d) fragments thereof. The promoter also can modulate transcription in a host genome in cis- or in trans-. In an organism such as a plant, the promoters and promoter control elements of the instant invention are useful to produce preferential transcription which results in a desired pattern of transcript levels in particular cells, tissues or organs or under particular conditions.

3. Table of Contents The following description of the present invention is outlined in the following table of contents.

A. Identifying and Isolating Promoter Sequences of the Invention (1) Cloning Methods (2) Chemical Synthesis B. Isolating Related Promoter Sequences (1) Relatives Based on Nucleotide Sequence Identity (2) Relatives Based on Coding Sequence Identity (3) Relatives Based on Common Function C. Identifying Control Elements ( 1 ) Types of Transcription Control Elements (2) Those Described by the Examples (3) Those Identifiable by Bioinformatics (4) Those Identifiable by In Vitro and In Vivo Assays (5) Non-Natural Control Elements D. Constructing Promoters and Control Elements (1) Combining Promoters and Promoter Control Elements (2) Number of Promoter Control Elements (3) Spacing Between Control Elements E. Vectors (1) Modification of Transcription by Promoters and Promoter Control Elements (2) Polynucleotide to be Transcribed (3) Other Regulatory Elements (4) Other Components of Vectors F. Insertion of Polynucleotides and Vectors Into a Host Cell (1) Autonomous of the Host Genome (2) Integrated into the Host Genome G. Utility A. Identifying and Isolating Promoter Sequences of the Invention The promoters and promoter control elements of the present invention are presented in the Sequence Listing and were identified from Arabidopsis thaliana or Oryza sativa. Additional promoter sequences encompassed by the invention can be identified as described below.

(1) Cloning Methods Isolation from genomic libraries of polynucleotides comprising the sequences of the promoters and promoter control elements of the present invention is possible using known techniques. For example, polymerase chain reaction (PCR) can amplify the desired polynucleotides utilizing primers designed from sequences in the row titled "Sequences". Polynucleotide libraries comprising genomic sequences can be constructed according to Sambrook et al., Molecular Cloning: A Laboratory Manual 2nd Ed. (1989) Cold Spring Harbor Press, Cold Spring Harbor, NY), for example. Other procedures for isolating polynucleotides comprising the promoter sequences of the invention include, without limitation, tail-PCR and 5' rapid amplification of cDNA ends (RACE). For tail-PCR see, for example, Liu et al. (1995) Plant J 8(3): 457-463; Liu et al. (1995) Genomics 25: 674-681; Liu et al. (1993) Nucl. Acids Res. 21(14): 3333-3334; and Zoe et al. (1999) BioTechniques 27(2): 240-248; for RACE see, for example, PCR Protocols: A Guide to Methods and Applications, (1990) Academic Press, Inc.

(2) Chemical Synthesis In addition, the promoters and promoter control elements described in the Sequence Listing can be chemically synthesized according to techniques in common use. See, for example, Beaucage et al. (1981) Tet. Lett. 22: 1859 and U.S. Pat. No. 4,668,777. Such chemical oligonucleotide synthesis can be carried out using commercially available devices, such as Biosearch 4600 or 8600 DNA synthesizer by Applied Biosystems, a division of Perkin-Elmer Corp. (Foster City, California, USA) and Expedite by Perceptive Biosystems (Framingham, Massachusetts, USA). Synthetic RNA, including natural and/or analog building blocks, can be synthesized on the Biosearch 8600 machines (see above). Oligonucleotides can be synthesized and then ligated together to construct the desired polynucleotide.

B. Isolating Related Promoter Sequences Included in the present invention are promoter and promoter control elements that are related to those described in the Sequence Listing. Such related sequences can be isolated using (a) nucleotide sequence identity, (b) coding sequence identity or (c) common function or gene products. Relatives can include both naturally occurring promoters and non-natural promoter sequences. Non-natural related promoters include nucleotide substitutions, insertions or deletions of naturally-occurring promoter sequences that do not substantially affect transcription modulation activity. For example, the binding of relevant DNA binding proteins can still occur with the non-natural promoter sequences and promoter control elements of the present invention. According to current knowledge, promoter sequences and promoter control elements exist as functionally important regions, such as protein binding sites and spacer regions. These spacer regions are apparently required for proper positioning of the protein binding sites. Thus, nucleotide substitutions, insertions and deletions can be tolerated in the spacer regions to a certain degree without loss of function. In contrast, less variation is permissible in the functionally important regions since changes in the sequence can interfere with protein binding. Nonetheless, some variation in the functionally important regions is permissible so long as function is conserved. The effects of substitutions, insertions and deletions to the promoter sequences or promoter control elements may be to increase or decrease the binding of relevant DNA binding proteins to modulate transcript levels of a polynucleotide to be transcribed. Effects may include tissue-specific or condition-specific modulation of transcript levels of the polypeptide to be transcribed. Polynucleotides representing changes to the nucleotide sequence of the DNA-protein contact region by insertion of additional nucleotides, changes to identity of relevant nucleotides, including use of chemically-modified bases, or deletion of one or more nucleotides are considered encompassed by the present invention.

(1) Relatives Based on Nucleotide Sequence Identity Included in the present invention are promoters exhibiting nucleotide sequence identity to those described in the Sequence Listing.

Definition Typically, such related promoters exhibit at least 80% sequence identity, preferably at least 85%, more preferably at least 90%, and most preferably at least 95%, even more preferably, at least 96%, 97%, 98% or 99% sequence identity compared to those shown in the Sequence Listing. Such sequence identity can be calculated by the algorithms and computers programs described above. Usually, such sequence identity is exhibited in an alignment region that is at least 75% of the length of a sequence shown in the Sequence Listing or corresponding full-length sequence; more usually at least 80%; more usually, at least 85%, more usually at least 90%, * and most usually at least 95%, yet even more usually, at least 96%, 97%, 98% or 99% of the length of a sequence shown in the Seqeunce Listing. The percentage of the alignment length is calculated by counting the number of bases of the sequence in the region of strongest alignment, e.g. a continuous region of the sequence that contains the greatest number of bases that are identical to the bases between two sequences that are being aligned. The number of bases in the region of strongest alignment is divided by the total base length of a sequence in the Sequence Listing. These related promoters generally exhibit similar preferential transcription as those promoters described in the Sequence Listing and as described in the "observed expression pattern" and "expected expression pattern" fields of the reports of Table 1.

Construction of Polynucleotides Naturally occurring promoters that exhibit nucleotide sequence identity to those shown in the Sequence Listing can be isolated using the techniques as described above. More specifically, such related promoters can be identified, for example, with typical hybridization procedures such as Southern blots or probing of polynucleotide libraries using varying stringencies (see above). Non-natural promoter variants of those shown in the Sequence Listing can be constructed using cloning methods that incorporate the desired nucleotide variation. For example see Ho et al. (1989) Gene 77:51-59, which describes a site directed mutagenesis procedure using PCR. Any related promoter showing sequence identity to those shown in the Sequence Listing can be chemically synthesized as described above. Also, the present invention includes non-natural promoters that exhibit the above- sequence identity to those in the Sequence Listing. The promoters and promoter control elements of the present invention may also be synthesized with 5' or 3' extensions to facilitate additional manipulation, for instance. Testing of Polynucleotides Polynucleotides of the invention were tested for activity by cloning the sequence into an appropriate vector, transforming plants with the construct and assaying for marker gene expression. Recombinant DNA constructs were prepared which comprise the polynucleotide sequences of the invention inserted into a vector suitable for transformation of plant cells. The construct can be made using standard recombinant DNA techniques (Sambrook et al. 1989) and can be introduced to the species of interest by Agi'obacterium-mediaϊQά transformation or by other means of transformation as referenced below. The vector backbone can be any of those typical in the art such as plasmids, viruses, artificial chromosomes, BACs, YACs and PACs and vectors of the sort described by (a) BAC: Shizuya et al. (1992) Proc. Natl. Acad. Sci. USA 89: 8794-8797; Hamilton et al. (1996) Proc. Natl. Acad. Sci. USA 93: 9975-9979; (b) YAC: Burke et al. (1987) Science 236:806-812; (c) PAC: Sternberg N. et al. (1990) Proc Natl Acad Sci U S A. 87(l):103-7; (d) Bacteria-Yeast Shuttle Vectors: Bradshaw et al. (1995) Nucl Acids Res 23 : 4850- 4856; (e) Lambda Phage Vectors: Replacement Vector, e.g., Frischauf et al. (1983) J. MoI Biol 170: 827-842; or Insertion vector, e.g., Huynh et al., In: Glover NM (ed) DNA Cloning: A practical Approach, VoLl Oxford: IRL Press (1985); T-DNA gene fusion vectors: Walden et al. (1990) MoI Cell Biol 1: 175-194; and (g) Plasmid vectors: Sambrook et al., infra. Typically, the construct comprises a vector containing a sequence of the present invention operationally linked to any marker gene. The polynucleotide was identified as a promoter by the expression of the marker gene. Although many marker genes can be used, Green Fluroescent Protein (GFP) is preferred. The vector may also comprise a marker gene that confers a selectable phenotype on plant cells. The marker may encode biocide resistance, particularly antibiotic resistance, such as resistance to kanamycin, G418, bleomycin, hygromycin or herbicide resistance, such as resistance to chlorosulfuron or phosphinotricin. Vectors can also include origins of replication, scaffold attachment regions (SARs), markers, homologous sequences, introns, etc.

Promoter Control Elements of the Invention The promoter control elements of the present invention include those that comprise a sequence shown in the Sequence Listing or fragments thereof. The size of the fragments can range from 5 bases to 10 kilobases (kb). Typically, the fragment size is no smaller than 8 bases; more typically, no smaller than 12; more typically, no smaller than 15 bases; more typically, no smaller than 20 bases; more typically, no smaller than 25 bases; even more typically, no more than 30, 35, 40 or 50 bases. Usually, the fragment size is no larger than 5 kb bases; more usually, no larger than 2 kb; more usually, no larger than 1 kb; more usually, no larger than 800 bases; more usually, no larger than 500 bases; even more usually, no more than 250, 200, 150 or 100 bases.

Relatives Based on Nucleotide Sequence Identity Included in the present invention are promoter control elements exhibiting nucleotide sequence identity to those described in the Sequence Listing or fragments thereof. Typically, such related promoters exhibit at least 80% sequence identity, preferably at least 85%, more preferably at least 90%, and most preferably at least 95%, even more preferably, at least 96%, 97%, 98% or 99% sequence identity compared to those shown in the Seqeunce Listing. Such sequence identity can be calculated by the algorithms and computers programs described above.

Promoter Control Element Configuration A common configuration of the promoter control elements in RNA polymerase II promoters is described in "Models for prediction and recognition of eukaryotic promoters", T. Werner (1999) Mammalian Genome 10, 168-175. Promoters are generally modular in nature. Promoters can consist of a basal promoter which functions as a site for assembly of a transcription complex comprising an RNA polymerase, for example RNA polymerase II. A typical transcription complex will include additional factors such as TF11B, TF11D and TFnE. Of these, TFnD appears to be the only one to bind DNA directly. The promoter might also contain one or more promoter control elements such as the elements discussed above. These additional control elements may function as binding sites for additional transcription factors that have the function of modulating the level of transcription with respect to tissue specificity, of transcriptional responses to particular environmental or nutritional factors and the like. One type of promoter control element is a polynucleotide sequence representing a binding site for proteins. Typically, within a particular functional module, protein binding sites constitute regions of 5 to 60, preferably 10 to 30, more preferably 10 to 20 nucleotides. Within such binding sites, there are typically 2 to 6 nucleotides which specifically contact amino acids of the nucleic acid binding protein. The protein binding sites are usually separated from each other by 10 to several hundred nucleotides, typically by 15 to 150 nucleotides, often by 20 to 50 nucleotides. Further, protein binding sites in promoter control elements often display dyad symmetry in their sequence. Such elements can bind several different proteins and/or a plurality of sites can bind the same protein. Both types of elements may be combined in a region of 50 to 1,000 base pairs.

Binding sites for any specific factor have been known to occur almost anywhere in a promoter. For example, functional AP-I binding sites can be located far upstream, as in the rat bone sialoprotein gene where an AP-I site located about 900 nucleotides upstream of the transcription start site suppresses expression (Yamauchi et al. (1996) Matrix Biol. 15, 119- 130). Alternatively, an AP-I site located close to the transcription start site plays an important role in the expression of Moloney murine leukemia virus (Sap et al. (1989) Nature 340, 242-244.

(2) Those Identifiable by Bioinformatics Promoter control elements from the promoters of the instant invention can be identified utilizing bioinformatic or computer driven techniques. One method uses a computer program AlignACE to identify regulatory motifs in genes that exhibit common preferential transcription across a number of time points. The program identifies common sequence motifs in such genes. See, Roth et al. (1998) Nature Biotechnol. 16: 949-945; Tavazoie et al. (1999) Nat Genet Jul;22 (3):281-5; Genomatix also makes available a GEMS Launcher program and other programs to identify promoter control elements and configuration of such elements. Genomatix is located in Munich, Germany. Other references also describe detection of promoter modules by models independent of overall nucleotide sequence similarity. See, for instance, Klingenhoff et al. (1999) Bioinformatics 15: 180-186. Protein binding sites of promoters can be identified as reported in "Computer-assisted prediction, classification, and delimination of protein binding sites in nucleic acids", Freeh et al. (1993) Nucleic Acids Research 21(7): 1655-1664. Other programs used to identify protein binding sites include, for example, Signal Scan (Prestridge et al. (1996) Comput. Appl. Biosci. 12: 157-160); Matrix Search (Chen et al. (1995) Comput. Appl. Biosci. 11: 563-566), available as part of Signal Scan 4.0; Matlnspector (Ghosh et al. (1993) Nucl. Acid Res. 21: 3117-3118) available via the internet; Conslnspector (Freeh et al. (1993) Nucl. Acids Res. 21: 1655-1664), available via the internet; TFSearch and TESS. Freeh et al. (1997) "Software for the analysis of DNA sequence elements of transcription", Bioinformatics & Sequence Analysis, Vol. 13, no. 1, 89-97 is a review of different software for analysis of promoter control elements. This paper also reports the usefulness of matrix-based approaches to yield more specific results. For other procedures, see Fickett et al. (2000) Curr. Op. Biotechnol. 11: 19-24 and Quandt et al. (1995) Nucleic Acids Res. 23: 4878-4884.

(3) Those Identifiable by In- Vitro and In- Vivo Assays Promoter control elements can also be identified with in-vitro assays such as transcription detection methods and with in-vivo assays such as enhancer trapping protocols.

In-Vitro Assays Examples of in-vitro assays include detection of binding of protein factors that bind promoter control elements. Fragments of the instant promoters can be used to identify the location of promoter control elements. Another option for obtaining a promoter control element with desired properties is to modify known promoter sequences. This is based on the fact that the function of a promoter is dependent on the interplay of regulatory proteins that bind to specific, discrete nucleotide sequences ("motifs") in the promoter. Such interplay subsequently affects the general transcription machinery and regulates transcription efficiency. These regulatory proteins are positive regulators or negative regulators (repressors) and one protein can have a dual role depending on the context (Johnson and McKnight, (1989) Annu. Rev. Biochem. 58:799-839). One type of in-vitro assay uses a known DNA binding factor to isolate DNA fragments that bind. If a fragment or promoter variant does not bind, then a promoter control element has been removed or disrupted. For specific assays, see, for instance, Luo et al. (1997) J. MoI. Biol. 266:470, Chusacultanachai et al. (1999) J. Biol. Chem. 274:23591, Fabbro et al. (1995) Biochem. Biophys. Res. Comm. 213:781). Alternatively, a DNA fragment suspected of conferring a particular pattern of expression can be examined for the ability to bind transcription factors responsible for generating the particular pattern using methods such as DNA footprinting (e.g. Cousins et al. (2000) Immunology 99:101 and V. Kolla et al. (1999) Biochem. Biophys. Res. Comm. 266:5) or "mobility-shift" assays (Fabiani et al. (2000) J. Biochem. 347:147 and Sugiura et al. (2000) J. Biochem 347: 155) or fluorescence polarization (e.g. Royer et al. U.S. Patent 5,445,935). Both mobility shift and DNA footprinting assays can also be used to identify portions of large DNA fragments that are bound by proteins in unpurified transcription extracts prepared from tissues or organs of interest. Cell-free transcription extracts can be prepared and used to directly assay in a reconstitutable system (Narayan et al. (2000) Biochemistry 39:818).

In-Vivo Assays Promoter control elements can be identified with reporter genes in in-vivo assays with the use of fragments of the instant promoter, polynucleotides or variants thereof. That is, a fragment(s) comprising a basal or "core" promoter operably linked to a reporter sequence can be inserted into a vector. When transcribed, a detectable label is produced. Examples of reporter genes include those encoding luciferase, green fluorescent protein, GUS, neo, cat and bar. Alternatively, the transcribed reporter sequence can be detected with AFLP and microarray techniques. In promoter probe vector systems, genomic DNA fragments are inserted upstream of the coding sequence of a reporter gene which is expressed only when the cloned fragment contains DNA having transcription modulation activity (Neveet al. (1979) Nature 277:324- 325). No transcription occurs when control elements are present in the fragment or when control elements present are disrupted. Probe vectors have been designed for assaying transcription modulation in E. coli (An et al. (1979) J. Bact. 140:400-407) and other bacterial hosts (Band et al. (1983) Gene 26:313-315 and Achen (1986) Gene 45:45-49), yeast (Goodey et al. (1986) MoI. Gen. Genet. 204:505-511) and mammalian cells (Pater et al. (1984) J. MoI. App. Gen. 2:363-371). A different design of a promoter/control element trap includes packaging into retroviruses for more efficient delivery into cells. One type of retroviral enhancer trap was described by von Melchner et al. (Genes Dev. (1992); U.S. Pat. # 5,364,783). The basic design of this vector includes a reporter protein coding sequence engineered into the U3 portion of the 3' LTR. No splice acceptor consensus sequences are included, limiting its utility to work as an enhancer trap only. A different approach to a gene trap using retroviral vectors was pursued by Friedrich and Soriano (Genes Dev. 1991) who engineered a lacZ-neo fusion protein linked to a splicing acceptor. LacZ-neo fusion protein expression from trapped loci allows not only for drug selection, but also for visualization of β-galatactosidase expression using the chromogenic substrate, X-gal. A general review of tools for identifying transcriptional regulatory regions of genomic DNA is provided by J. W. Fickett et al. (Curr. Opn. Biotechnol (2000) 11:19).

(4) Non-Natural Control Elements Non-natural control elements can be constructed by inserting, deleting or substituting nucleotides into the promoter control elements described above. Such control elements are capable of transcription modulation that can be determined using any of the assays described above.

C. Constructing Promoters with Control Elements (1) Combining Promoters and Promoter Control Elements The promoter polynucleotides and promoter control elements of the present invention, both naturally occurring and synthetic, can be combined with each other to produce the desired preferential transcription. In addition, the polynucleotides of the invention can be combined with other known sequences to generate promoters useful for modulating, for example, tissue-specific transcription or condition-specific transcription. Such preferential transcription can be determined using the techniques or assays described above. The relatives, fragments and variants as well as full-length sequences shown in the Sequence Listing are useful alone or in combination. The location and relation of promoter control elements within a promoter can affect the ability of the promoter to modulate transcription. The order and spacing of control elements is a factor when constructing promoters.

(2) Number of Promoter Control Elements Promoters can contain any number of control elements. For example, a promoter can contain multiple transcription binding sites or other control elements. One element may confer tissue or organ specificity, another element may limit transcription to specific time periods, etc. Typically, promoters will contain at least a basal or core promoter as described above. Any additional element can be included as desired. For example, a fragment comprising a basal or "core" promoter can be fused with another fragment with any number of additional control elements.

(3) Spacing Between Control Elements Spacing between control elements or the configuration or control elements can be determined or optimized to permit the desired polynucleotide or protein-polynucleotide interactions to occur. For example, if two transcription factors bind to a promoter simultaneously or relatively close in time, the binding sites are spaced to allow each factor to bind without steric hinderance. The spacing between two such hybridizing control elements can be as small as a profile of a protein bound to a control element. In some cases, two protein binding sites can be adjacent to each other when the proteins bind at different times during the transcription process. Further, when two control elements hybridize the spacing between such elements will be sufficient to allow the promoter polynucleotide to form a hairpin or loop so as to permit the two elements to bind. The spacing between two such hybridizing control elements can be as small as a t-RNA loop, to as large as 10 kb. Typically, the spacing is no smaller than 5 bases, more typically no smaller than 8, more typically no smaller than 15 bases, more typically no smaller than 20 bases, more typically no smaller than 25 bases, even more typically no more than 30, 35, 40 or 50 bases. Usually, the fragment size in no larger than 5 kb bases, more usually no larger than 2 kb, more usually no larger than 1 kb, more usually no larger than 800 bases, more usually no larger than 500 bases, even more usually no more than 250, 200, 150 or 100 bases. Such spacing between promoter control elements can be determined using the techniques and assays described above.

(4) Other Promoters The following are promoters that are induced under stress conditions and can be combined with those of the present invention: ldhl (oxygen stress, tomato see Germain and Ricard (1997) Plant MoI Biol 35:949-54), GPx and CAT (oxygen stress, mouse, see Franco et al. (1999) Free Radic Biol Med 27: 1122-32), ci7 (cold stress, potato, see Kirch et al. (1997) Plant MoI Biol. 33:897-909), Bz2 (heavy metals, maize, see Marrs and Walbot (1997) Plant Physiol 113:93-102), HSP32 (hyperthermia, rat, see Raju and Maines (1994) Biochim Biophys Acta 1217:273-80); MAPKAPK-2 (heat shock, Drosophila, see Larochelle and Suter (1995) Gene 163:209-14). In addition, the following promoters are examples those induced by the presence or absence of light and can be used in combination with those of the present invention: Topoisomerase II (pea, see Reddy et al. (1999) Plant MoI Biol 41: 125-37), chalcone synthase (soybean, see Wingender et al. (1989) MoI Gen Genet 218:315-22), mdm2 gene (human tumor, see Saucedo et al. (1998) Cell Growth Differ 9: 119-30), Clock and BMALl (rat, see Namihira et al. (1999) Neurosci Lett 271 :1-4), PHYA (Arabidopsis, see Canton and Quail 1999 Plant Physiol 121: 1207-16), PRB-Ib (tobacco, see Sessa et al. (1995) Plant MoI Biol 28:537-47) and YprlO (common bean, see Walter et al. (1996) Eur J Biochem 239:281-93). The promoters and control elements of the following genes can be used in combination with the present invention to confer tissue specificity: for roots MipB (iceplant, Yamada et al. (1995) Plant Cell 7: 1129-42) and SUCS (root nodules, broadbean, Kuster et al. (1993) MoI Plant Microbe Interact 6:507-14), for leaves OsSUTl (rice, Hirose et al. (1997) Plant Cell Physiol 38: 1389-96), for siliques Msg (soybean, Stomvik et al. (1999) Plant MoI Biol 41:217-31) and for inflorescence (Arabidopsis, Shani et al. (1997) Plant MoI Biol 34(6):837-42) and ACTl 1 (Arabidopsis, Huang et al. (1997) Plant MoI Biol 33:125-39). Still other promoters are affected by hormones or participate in specific physiological processes, which can be used in combination with those of present invention. Some examples are the ACC synthase gene that is induced differently by ethylene and brassinosteroids (mung bean, Yi et al. (1999) Plant MoI Biol41 :443-54), the TAPGl gene that is active during abscission (tomato, Kalaitzis et al. (1995) Plant MoI Biol 28:647-56) and the 1- aminocyclopropane-l-carboxylate synthase gene (carnation, Jones et al. (1995) Plant MoI Biol 28:505-12) and the CP-2/cathepsin L gene (rat, Kim and Wright (1997) Biol Reprod 57:1467-77), which are both active during senescence.

E. Vectors Vectors are a useful component of the present invention. In particular, vectors can deliver the present promoters and/or promoter control elements to a cell. For the purposes of this invention, such delivery ranges from randomly introducing the promoter or promoter control element alone into a cell to integrating the vector containing the promoter or promoter control element into a cell's genome. Thus, a vector need not be limited to a DNA molecule such as a plasmid, cosmid or bacterial phage that has the capability of replicating autonomously in a host cell. All other manner of delivery of the promoters and promoter control elements of the invention are envisioned. The various T-DNA vector types are preferred vectors for use with the present invention. Many useful vectors are commercially available. It may also be useful to attach a marker sequence to the present promoter and promoter control element in order to determine activity of such sequences. Marker sequences typically include genes that provide antibiotic resistance, such as tetracycline resistance, hygromycin resistance or ampicillin resistance, or provide herbicide resistance. Specific selectable marker genes may be used to confer resistance to herbicides such as glyphosate, glufosinate orbroxynil (Comai et al. (1985) Nature 317: 741-744; Gordon-Kamm et al. (1990) Plant Cell 2: 603-618; and Stalker et al. (1988) Science 242: 419-423). Other marker genes exist which provide hormone responsiveness.

(1) Modification of Transcription by Promoters and Promoter Control Elements The promoter or promoter control element of the present invention may be operably linked to a polynucleotide to be transcribed. In this manner, the promoter or promoter control element modifys transcription by modulating transcript levels of that polynucleotide when inserted into a genome. The promoter or promoter control element need not be linked, operably or otherwise, to a polynucleotide to be transcribed before being inserted into a genome. For example, the promoter or promoter control element can be inserted into the genome in front of a polynucleotide already present therein. Here, the promoter or promoter control element modulates the transcription of a polynucleotide that was already present in the genome. This polynucleotide may be native to the genome or inserted at an earlier time. Alternatively, the promoter or promoter control element can simply be inserted into a genome or maintained extrachromosomally as a way to divert the transcription resources of the system to itself. See, for example^ Vaucheret et al. (1998) Plant J 16: 651-659. This approach may be used to downregulate the transcript levels of a group of polynucleotide(s).

(2) Polynucleotide to be Transcribed The nature of the polynucleotide to be transcribed is not limited. Specifically, the polynucleotide may include sequences that will have activity as RNA as well as sequences that result in a polypeptide product. These sequences may include, but are not limited to antisense sequences, ribozyme sequences, spliceosomes, amino acid coding sequences and fragments thereof. Specific coding sequences may include, but are not limited to endogenous proteins or fragments thereof, or heterologous proteins including marker genes or fragments thereof. Promoters and control elements of the present invention are useful for modulating metabolic or catabolic processes. Such processes include, but are not limited to secondary product metabolism, amino acid synthesis, seed protein storage, oil development, pest defense and nitrogen usage. Some examples of genes, transcripts, peptides or polypeptides participating in these processes which can be modulated by the present invention: are tryptophan decarboxylase (tdc), strictosidine synthase (strl), dihydrodipicolinate synthase (DHDPS), aspartate kinase (AK), 2S albumin, alpha-, beta-, and gamma-zeins, ricinoleate, 3- ketoacyl-ACP synthase (KAS), Bacillus thuringiensis (Bt) insecticidal protein, cowpea trypsin inhibitor (CpTI), asparagine synthetase and nitrite reductase. Alternatively, expression constructs can be used to inhibit expression of these peptides and polypeptides by incorporating the promoters in constructs for antisense use, co-suppression use or for the production of dominant negative mutations.

(3) Other Regulatory Elements As explained above, several types of regulatory elements exist concerning transcription regulation. Each of these regulatory elements may be combined with the present vector if desired.

(4) Other Components of Vectors Translation of eukaryotic mRNA is often initiated at the codon that encodes the first methionine. Thus, when constructing a recombinant polynucleotide for expressing a protein product according to the present invention, it is preferable to ensure that no intervening codons encoding a methionine are contained within the linkage between the polynucleotide to be transcribed, or a functional derivative thereof, and the 3 ' portion of the promoter, preferably including the TATA box. The vector of the present invention may contain additional components. For example, . an origin of replication that allows for replication of the vector in a host cell may be added. In addition, homologous sequences flanking a target location in the genome may be added to allow for site-specific recombination of a specific sequence contained in the vector. T-DNA sequences also allow for insertion of a specific sequence randomly into a target genome, but in a random manner. The vector may also contain a plurality of restriction sites for insertion of the promoter and/or promoter control elements of the present invention as well as any polynucleotide to be transcribed. The vector can additionally contain selectable marker genes. The vector can also contain a transcriptional and translational initiation region and/or a transcriptional and translational termination region that functions in the host cell. The termination region may be native with the transcriptional initiation region, may be native with the polynucleotide to be transcribed or may be derived from another source. Convenient termination regions are available from the Ti-plasmid of A. tumefaciens, such as the octopine synthase and nopaline synthase termination regions. See also, Guerineau et al. (1991) MoI. Gen. Genet. 262:141-144; Proudfoot (1991) Cell 64:671-674; Sanfacon et al. (1991) Genes Dev. 5:141-149; Mogen et al. (1990) Plant Cell 2:1261-1272; Munroe et al. (1990) Gene 91:151-158; Ballas et al. (1989) Nucleic Acids Res. 17:7891-7903; Joshi et al. (1987) Nucleic Acid Res. 15:9627-9639. Where appropriate, the polynucleotide to be transcribed may be optimized for increased expression in a certain host cell. For example, the polynucleotide can be synthesized using preferred codons for improved transcription and translation. See U.S. Patent Nos. 5,380,831, 5,436, 391 and Murray et al. (1989) Nucleic Acids Res. 17:477-498. Additional sequence modifications include elimination of sequences encoding spurious polyadenylation signals, exon intron splice site signals, transposon-like repeats and other such sequences well characterized as deleterious to expression. The G-C content of the polynucleotide may be adjusted to the average levels for a given cellular host, as calculated by reference to known genes expressed in the host cell. The polynucleotide sequence may be modified to avoid hairpin secondary mRNA structures. A general description of expression vectors and reporter genes can be found in Gruber et al., "Vectors for Plant Transformation", in Methods in Plant Molecular Biology & Biotechnology (1993) Glich et al. eds., pp. 89-119, CRC Press). Moreover GUS expression vectors and GUS gene cassettes are available from Clonetech Laboratories, Inc. (Palo Alto, California) while luciferase expression vectors and luciferase gene cassettes are available from Promega Corp. (Madison, Wisconsin). GFP vectors are available from Aurora Biosciences.

F. Polynucleotide Insertion Into A Host Cell The polynucleotides according to the present invention can be inserted into a host cell. A host cell includes but is not limited to a plant, mammalian, insect, yeast and prokaryotic cell, preferably a plant cell. The method of insertion into the host cell genome is chosen based on convenience. For example, the insertion into the host cell genome may either be accomplished by vectors that integrate into the host cell genome or by vectors which exist independent of the host cell genome.

Cl) Polynucleotides Autonomous of the Host Genome The polynucleotides of the present invention can exist autonomously or independent of the host cell genome. Vectors of these types are known in the art and include, for example, certain types of non-integrating viral vectors, autonomously replicating plasmids, artificial chromosomes and the like. Additionally, in some cases transient expression of a polynucleotide may be desired.

(2) Polynucleotides Integrated into the Host Genome The promoter sequences, promoter control elements or vectors of the present invention can be transformed into host cells. These transformations can be into protoplasts or isolated cells or intact tissues. Preferably, expression vectors are introduced into intact tissue. General methods of culturing plant tissues are provided for example by Maki et al. ("Procedures for Introducing Foreign DNA into Plants" in Methods in Plant Molecular Biology & Biotechnology (1993) Glich et al. eds., pp. 67-88 CRC Press) and by Phillips et al. "Cell-Tissue Culture and In- Vitro Manipulation" in Corn & Corn Improvement, 3rd Edition lOSprague et al. (1998) eds. pp. 345-387) American Society of Agronomy Inc. et al. Methods of introducing polynucleotides into plant tissue include the direct infection or co-cultivation of a plant cell v/iHhAgrobacterium tumefaciens (Horsch et al. (1985) Science 227:1229). Descriptions of Agrobacterium vector systems and methods for Agrobacterium- mediated gene transfer are provided by Gruber et al. supra. Alternatively, polynucleotides are introduced into plant cells or other plant tissues using a direct gene transfer method such as microprojectile-mediated delivery, DNA injection, electroporation and the like. More preferably, polynucleotides are introduced into plant tissues using the microprojectile media delivery with the biolistic device. See, for example, Tomes et al., "Direct DNA transfer into intact plant cells via microprojectile bombardment" In: Plant Cell, Tissue and Organ Culture: Fundamental Methods (1995) Gamborg and Phillips eds. Springer Verlag, Berlin. In another embodiment of the current invention, expression constructs can be used for gene expression in callus culture for the purpose of expressing marker genes encoding peptides or polypeptides that allow identification of transformed plants. Here, a promoter that is operatively linked to a polynucleotide to be transcribed is transformed into plant cells and the transformed tissue is then placed on callus-inducing media. If the transformation is conducted with leaf discs, for example, callus will initiate along the cut edges. Once callus growth has initiated, callus cells can be transferred to callus shoot-inducing or callus root- inducing media. Gene expression will occur in the callus cells developing on the appropriate media: callus root-inducing promoters will be activated on callus root-inducing media, etc. Examples of such peptides or polypeptides useful as transformation markers include, but are not limited to barstar, glyphosate, chloramphenicol acetyltransferase (CAT), kanamycin, spectinomycin, streptomycin or other antibiotic resistance enzymes, green fluorescent protein (GFP) and β-glucuronidase (GUS), etc. Some of the promoters in the Seqeunce Listing will also be capable of sustaining expression in some tissues or organs after the initiation or completion of regeneration. Examples of these tissues or organs are somatic embryos, cotyledon, hypocotyl, epicotyl, leaf, stems, roots, flowers and seed. Integration into the host cell genome also can be accomplished by methods known in the art such as by homologous sequences or T-DNA discussed above or by using the cre-lox system (Vergunst et al. (1998) Plant MoI. Biol. 38:393).

G. Utility Common Uses In yet another embodiment, the promoters of the present invention can be used to further understand developmental mechanisms. For example, promoters that are specifically induced during callus formation, somatic embryo formation, shoot formation or root formation can be used to explore the effects of overexpression, repression or ectopic expression of target genes, or for isolation of trans-acting factors. The vectors of the invention can be used not only for expression of coding regions but may also be used in exon-trap cloning, or promoter trap procedures to detect differential gene expression in various tissues. See Lindsey et al (1993) "Tagging Genomic Sequences That Direct Transgene Expression by Activation of a Promoter Trap in Plants," Transgenic Research 2:3347 and Auch et al. "Exon Trap Cloning: Using PCR to Rapidly Detect and Clone Exons from Genomic DNA Fragments," Nucleic Acids Research, 18:674. Entrapment vectors, first described for use in bacteria (Casadaban and Cohen (1979) Proc. Nat. Aca. Sci. U.S.A. 76: 4530 and Casadaban et al. (1980) J. Bacterid. 143: 971) permit selection of insertional events that lie within coding sequences. Entrapment vectors can be introduced into pluripotent ES cells in culture and then passed into the germline via chimeras (Gossler et al. (1989) Science 244: 463 and Skarnes (1990) Biotechnology 8: 827). Promoter or gene trap vectors often contain a reporter gene, e.g. lacZ, lacking its own promoter and/or splice acceptor sequence upstream. That is, promoter gene traps contain a • reporter gene with a splice site but no promoter. If the vector lands in a gene and is spliced into the gene product, then the reporter gene is expressed. Recently, the isolation of preferentially-induced genes has been made possible with the use of sophisticated promoter traps (e.g. IVET) that are based on conditional auxotrophy complementation or drug resistance. In one IVET approach, various bacterial genome fragments are placed in front of a necessary metabolic gene that is coupled to a reporter gene. The DNA constructs are inserted into a bacterial strain otherwise lacking the metabolic gene and the resulting bacteria are used to infect the host organism. Only bacteria expressing the metabolic gene survive in the host organism. Consequently, inactive constructs can be eliminated by harvesting only bacteria that survive for some minimum period in the host. At the same time, constitutively active constructs can be eliminated by selecting only bacteria that do not express the reporter gene under laboratory conditions. The bacteria selected by such a method contain constructs that are selectively induced only during infection of the host. The rVεT approach can be modified in plants to identify genes induced in either the bacteria or the plant cells upon pathogen infection or root colonization. For information on rVET see the following articles: Mahan et al. (1993) Science 259:686-688, Mahan et al. (1995) PNAS USA 92:669-673, Heithoff et al. (1997) PNAS USA 94:934-939, and Wang et al. (1996) PNAS USA. 93:10434.

Constitutive Transcription Promoters and control elements providing constitutive transcription are desired for modulation of transcription in most cells of an organism under most environmental conditions. In a plant, for example, constitutive transcription is useful for modulating genes involved in defense, pest resistance, herbicide resistance, etc. Constitutive up-regulation and down-regulation of transcription are useful for these applications. For instance, genes, transcripts and/or polypeptides that increase defense, pest and herbicide resistance may require constitutive up-regulation of transcription. In contrast, constitutive down-regulation of transcriptional may be desired to inhibit those genes, transcripts, and/or polypeptides that lower defense, pest and herbicide resistance. Typically, promoter or control elements that provide constitutive transcription produce transcription levels that are statistically similar in many tissues and environmental conditions observed. Calculation of P- value from the different observed transcript levels is one means of determining whether a promoter or control element is providing constitutive up-regulation. P-value is the probability that the difference of transcript levels is not statistically significant. The higher the P-value, the more likely the difference of transcript levels is not significant. One formula used to calculate P-value is as follows: I φ(x) dx, integrated from a to ∞, where φ(x) is a normal distribution; where a = |Sx-μ| σ(all Samples except Sx); where Sx = the intensity of the sample of interest where μ = is the average of the intensities of all samples except Sx,

= (σS1... Sn)-Sx n-1 where σ(S 1... S 11 , not including Sx) = the standard deviation of all sample intensities except Sx. The P-value from the formula ranges from 1.0 to 0.0. Usually, each P-value of the transcript levels produced by the promoter or control element and observed in a majority of cells, tissues or organs under various environmental conditions is greater than 10"8; more usually, greater than 10"7; even more usually, greater than 10"6; even more usually, greater than 10"5 or 10"4. For up-regulation of transcription, promoter and control elements produce transcript levels that are above background of the assay.

Stress Induced Preferential Transcription Promoters and control elements providing modulation of transcription under oxidative, drought, oxygen, wound and methyl jasmonate stress are particularly useful for producing host cells or organisms that are more resistant to biotic and abiotic stresses. For example, in a plant modulation of genes, transcripts and/or polypeptides in response to oxidative stress can protect cells against damage caused by oxidative agents such as hydrogen peroxide and other free radicals. Drought induction of genes, transcripts and/or polypeptides are useful to increase the viability of a plant, for example when water is a limiting factor. In contrast, genes, transcripts and/or polypeptides induced during oxygen stress can help the flood tolerance of a plant. The promoters and control elements of the present invention can modulate the plant's response to stresses. Examples of some genes involved in stress condition responses are VuPLDl (drought stress, Cowpea; Phanα-Thi et al. (1999) Plant MoI. Biol 1257-65), pyruvate decarboxylase (oxygen stress, rice; Rivosal et al. (1997) Plant Physiol. 114(3): 1021-29), and the chromoplast specific carotenoid gene (oxidative stress, Capsicum; see Bouvier et al. (1998) J Biol Chem 273: 30651-59). Promoters and control elements providing preferential transcription during wounding or that are induced by methyl jasmonate can produce a defense response in host cells or organisms. In a plan, preferential modulation of genes, transcripts and/or polypeptides under such conditions is useful to induce a defense response to mechanical wounding, pest or pathogen attack or treatment with certain chemicals. Promoters and control elements of the present invention also can trigger a response similar to those described for cf9 (viral pathogen, tomato; O'Donnell et al. (1998) Plant J 14(1): 137-42), hepatocyte growth factor activator inhibitor type 1 (HAI-I), which enhances tissue regeneration (tissue injury, human; Koono et al. (1999) J Histochem Cytochem 47: 673-82), copper amine oxidase (CuAO) induced during ontogenesis and wound healing (wounding, chick-pea; Rea et al. (1998) FEBS Letters 437: 177-82), proteinase inhibitor II (wounding, potato; Pena-Cortes et al. (1988) Planta 174: 84-89), protease inhibitor II (methyl jasmonate, tomato; Farmer and Ryan (1990) Proc Natl Acad Sci USA 87: 7713-7716) and two vegetative storage protein genes VspA and VspB (wounding, jasmonic acid and water deficit; soybean; Mason and Mullet (1990) Plant Cell 2: 569-579). Up-regulation and down-regulation of transcription are useful for these applications. For instance, genes, transcripts and/or polypeptides that increase oxidative, flood or drought tolerance may require up-regulation of transcription. In contrast, transcriptional down- regulation may be desired to inhibit those genes, transcripts and/or polypeptides that lower such tolerance. Typically, promoter or control elements which provide preferential transcription in wounding or under methyl jasmonate induction produce transcript levels that are statistically significantly altered as compared to cell types, organs or tissues under other conditions. For preferential up-regulation of transcription, promoter and control elements produce transcript levels that are above background of the assay.

Light Induced Preferential Transcription Promoters and control elements providing preferential transcription when induced by light exposure can be utilized to modulate growth, metabolism and development to increase drought tolerance and to decrease damage from light stress for host cells or organisms. In a plant, modulation of genes, transcripts and/or polypeptides in response to light is useful (1) to increase the photosynthetic rate; (2) to increase storage of certain molecules in leaves or green parts only, e.g. silage with high protein or starch content; (3) to modulate production of exogenous compositions in green tissue, e.g. certain feed enzymes; (4) to induce growth or development, such as fruit development and maturity, during extended exposure to light; (5) to modulate guard cells to control the size of stomata in leaves to prevent water loss, or (6) to induce accumulation of beta-carotene to help plants cope with light induced stress. The promoters and control elements of the present invention can also trigger responses similar to those described for: abscisic acid iαsensitive3 (ABI3) (dark-grown Arabidopsis seedlings, Rohde et al. (2000) Plant Cell 12: 35-52), asparagine synthetase (pea root nodules, Tsai, Coruzzi, (1990) EMBO J 9: 323-32) and mdm2 gene (human tumor; Saucedo et al. (1998) Cell Growth Differ 9: 119-30). Up-regulation and down-regulation of transcription are useful for these applications. For instance, genes, transcripts and/or polypeptides that increase drought or light tolerance may require up-regulation of transcription. In contrast, transcriptional down-regulation may be desired to inhibit those genes, transcripts and/or polypeptides that lower such tolerance. Typically, promoter or control elements which provide preferential transcription in cells, tissues or organs exposed to light produce transcript levels that are statistically significantly altered as compared to cells, tissues or organs under decreased light exposure (intensity or length of time). For preferential up-regulation of transcription, promoter and control elements produce transcript levels that are above background of the assay. Dark Induced Preferential Transcription Promoters and control elements providing preferential transcription when induced by dark or decreased light intensity or decreased light exposure time can be utilized to time growth, metabolism and development and to modulate photosynthesis capabilities for host cells or organisms. In a plant, modulation of genes, transcripts and/or polypeptides in response to dark is useful (1) to induce growth or development, such as fruit development and maturity, despite lack of light; (2) to modulate genes, transcripts and/or polypeptide active at night or on cloudy days; or (3) to preserve the plastid ultra structure present at the onset of darkness. The present promoters and control elements can also trigger response similar to those described in the section above. Up-regulation and down-regulation of transcription are useful for these applications. For instance, genes, transcripts and/or polypeptides that increase growth and development may require up-regulation of transcription. In contrast, transcriptional down-regulation may be desired to inhibit those genes, transcripts and/or polypeptides that modulate photosynthesis capabilities. Typically, promoter or control elements which provide preferential transcription under exposure to dark or decreased light intensity or decreased exposure time produce transcript levels that are statistically significantly altered. For preferential up-regulation of transcription, promoter and control elements produce transcript levels that are above background of the assay.

Leaf Preferential Transcription Promoters and control elements providing preferential transcription in a leaf can modulate growth, metabolism and development or modulate energy and nutrient utilization in host cells or organisms. In a plant, preferential modulation of genes, transcripts and/or polypeptide in a leaf is useful (1) to modulate leaf size, shape, and development; (2) to modulate the number of leaves ; or (3) to modulate energy or nutrient usage in relation to other organs and tissues. Up-regulation and down-regulation of transcription are useful for these applications. For instance, genes, transcripts and/or polypeptides that increase growth may require up- regulation of transcription. In contrast, transcriptional down-regulation may be desired to inhibit energy usage in a leaf and to redirect it to the fruit instead, for instance. Typically, promoter or control elements which provide preferential transcription in the cells, tissues, or organs of a leaf produce transcript levels that are statistically significantly altered as compared to other cells, organs or tissues. For preferential up-regulation of transcription, promoter and control elements produce transcript levels that are above background of the assay.

Root Preferential Transcription Promoters and control elements providing preferential transcription in a root can modulate growth, metabolism, development, nutrient uptake, nitrogen fixation or modulate energy and nutrient utilization in host cells or organisms. In a plant, for example, preferential modulation of genes, transcripts, and/or in a leaf, is useful (1) to modulate root size, shape, and development; (2) to modulate the number of roots, or root hairs; (3) to modulate mineral, fertilizer, or water uptake; (4) to modulate transport of nutrients; or (4) to modulate energy or nutrient usage in relation to other organs and tissues. Up-regulation and down-regulation of transcription are useful for these applications. For instance, genes, transcripts and/or polypeptides that increase growth may require up- regulation of transcription. In contrast, transcriptional down-regulation may be desired to inhibit nutrient usage in a root and to redirect it to the leaf instead, for instance. Typically, promoter or control elements which provide preferential transcription in cells, tissues or organs of a root produce transcript levels that are statistically significantly altered as compared to other cells, organs or tissues. For preferential up-regulation of transcription, promoter and control elements produce transcript levels that are above background of the assay. Stem/Shoot Preferential Transcription Promoters and control elements providing preferential transcription in a stem or shoot can modulate growth, metabolism and development or -modulate energy and nutrient utilization in host cells or organisms. In a plant, preferential modulation of genes, transcripts and/or a polypeptide in a stem or shoot is useful (1) to modulate stem/shoot size, shape, and development; or (2) to modulate energy or nutrient usage in relation to other organs and tissues Up-regulation and down-regulation of transcription are useful for these applications. For instance, genes, transcripts and/or polypeptides that increase growth may require up- regulation of transcription. In contrast, transcriptional down-regulation may be desired to inhibit energy usage in a stem/shoot and to redirect it to the fruit instead, for instance. Typically, promoter or control elements which provide preferential transcription in the cells, tissues or organs of a stem or shoot produce transcript levels that are statistically significant as compared to other cells, organs or tissues. For preferential up-regulation of transcription, promoter and control elements produce transcript levels that are above background of the assay.

Frnit and Seed Preferential Transcription Promoters and control elements providing preferential transcription in a silique or fruit can time growth, development, or maturity; or modulate fertility; or modulate energy and nutrient utilization in host cells or organisms. In a plan,t preferential modulation of genes, transcripts and/or polypeptides in a fruit is useful (1) to modulate fruit size, shape, development, and maturity; (2) to modulate the number of fruit or seeds; (3) to modulate seed shattering; (4) to modulate components of seeds, such as, storage molecules, starch, protein, oil, vitamins, anti-nutritional components, such as phytic acid; (5) to modulate seed and/or seedling vigor or viability; (6) to incorporate exogenous compositions into a seed, such as lysine rich proteins; (7) to permit similar fruit maturity timing for early and late blooming flowers; or (8) to modulate energy or nutrient usage in relation to other organs and tissues. Up-regulation and down-regulation of transcription are useful for these applications. For instance, genes, transcripts, and/or polypeptides that increase growth may require up- regulation of transcription. In contrast, transcriptional down-regulation may be desired to inhibit late fruit maturity, for instance. Typically, promoter or control elements which provide preferential transcription in the cells, tissues or organs of siliques or fruits produce transcript levels that are statistically significantly altered as compared to other cells, organs or tissues. For preferential up-regulation of transcription, promoter and control elements produce transcript levels that are above background of the assay.

Callus Preferential Transcription Promoters and control elements providing preferential transcription in a callus can be useful to modulating transcription in dedifferentiated host cells. In a plant transformation, for example, preferential modulation of genes or transcript in callus is useful to modulate transcription of a marker gene, which can facilitate selection of cells that are transformed with exogenous polynucleotides. Up-regulation and down-regulation of transcription are useful for these applications. For instance, genes, transcripts and/or polypeptides that increase marker gene detectabilitymay require up-regulation of transcription. In contrast, transcriptional down- regulation may be desired to increase the ability of the calluses to differentiate, for instance. Typically, promoter or control elements which provide preferential transcription in callus produce transcript levels that are statistically significantly altered as compared to other cell types, tissues, or organs. Calculation of P-value from the different observed transcript levels is one means of determining whether a promoter or control element is providing such preferential transcription. Usually, each P-value of the transcript levels observed in callus as compared to at least one other cell type, tissue or organ, is less than 10"4; more usually, less than 10~5; even more usually, less than 10"6; even more usually, less than 10~7 or 10'8. For preferential up-regulation of transcription, promoter and control elements produce transcript levels that are above background of the assay. Flower Specific Transcription Promoters and control elements providing preferential transcription in flowers can modulate pigmentation or modulate fertility in host cells or organisms. In a plant, preferential modulation of genes, transcripts and/or polypeptides in a flower is useful, (1) to modulate petal color; or (2) to modulate the fertility of pistil and/or stamen. Up-regulation and down-regulation of transcription are useful for these applications. For instance, genes, transcripts and/or polypeptides that increase pigmentation may require up-regulation of transcription. In contrast, transcriptional down-regulation may be desired to inhibit fertility, for instance. Typically, promoter or control elements which provide preferential transcription in flowers produce transcript levels that are statistically significantly altered as compared to other cells, organs or tissues. For preferential up-regulation of transcription, promoter and control elements produce transcript levels that are above background of the assay.

Immature Bud and Inflorescence Preferential Transcription Promoters and control elements providing preferential transcription in an immature bud or inflorescence can time growth, development or maturity or modulate fertility or viability in host cells or organisms. In a plant, preferential modulation of genes, transcripts, and/or polypeptide in an immature bud or inflorescence is useful, (1) to modulate embryo development, size, and maturity; (2) to modulate endosperm development, size, and composition; (3) to modulate the number of seeds and fruits; or (4) to modulate seed development and viability. Up-regulation and down-regulation of transcription is useful for these applications. For instance, genes, transcripts and/or polypeptides that increase growth may require up- regulation of transcription. In contrast, transcriptional down-regulation may be desired to decrease endosperm size, for instance. Typically, promoter or control elements which provide preferential transcription in immature buds and inflorescences produce transcript levels that are statistically significantly altered as compared to other cell types, organs or tissues. For preferential up-regulation of transcription, promoter and control elements produce transcript levels that are above background of the assay.

Senescence Preferential Transcription Promoters and control elements providing preferential transcription during senescence can be used to modulate cell degeneration, nutrient mobilization and scavenging of free radicals in host cells or organisms. Other types of responses that can be modulated include, for example, senescence associated genes (SAG) that encode enzymes thought to be involved in cell degeneration and nutrient mobilization (Arabidopsis; Hensel et al. (1993) Plant Cell 5: 553-64), and the CP-2/cathepsin L gene (rat; Kim and Wright (1997) Biol Reprod 57: 1467- 77). Both of these genes are induced during senescence. In a plant, preferential modulation of genes, transcripts and/or polypeptides during senescencing is useful to modulate fruit ripening. Up-regulation and down-regulation of transcription are useful for these applications. For instance, genes, transcripts and/or polypeptides that increase scavenging of free radicals may require up-regulation of transcription. In contrast, transcriptional down-regulation may be desired to inhibit cell degeneration, for instance. Typically, promoter or control elements which provide preferential transcription in cells, tissues or organs during senescence produce transcript levels that are statistically significantly altered as compared to other conditions. For preferential up-regulation of transcription, promoter and control elements produce transcript levels that are above background of the assay.

Germination Preferential Transcription Promoters and control elements providing preferential transcription in a germinating seed can time growth, development or maturity or modulate viability in host cells or organisms. In a plant, preferential modulation of genes, transcripts and/or polypeptide in a germinating seed is useful (1) to modulate the emergence of they hypocotyls, cotyledons and radical; or (2) to modulate shoot and primary root growth and development;

Up-regulation and down-regulation of transcription is useful for these applications. For instance, genes, transcripts and/or polypeptides that increase growth may require up- regulation of transcription. In contrast, transcriptional down-regulation may be desired to decrease endosperm size, for instance. Typically, promoter or control elements which provide preferential transcription in a germinating seed produce transcript levels that are statistically significantly altered as compared to other cell types, organs or tissues. For preferential up-regulation of transcription, promoter and control elements produce transcript levels that are above background of the assay.

RESULTS

GFP EXPERIMENTAL PROCEDURES AND RESULTS

PROCEDURES The polynucleotide sequences of the present invention were tested for promoter activity using Green Fluorescent Protein (GFP) assays in the following manner. Approximately 1-2 kb of genomic sequence occurring immediately upstream of the ATG translational start site of the gene of interest was isolated using appropriate primers tailed with BstXI restriction sites. Standard PCR reactions using these primers and genomic DNA were conducted. The resulting product was isolated, cleaved with BstXI and cloned into the BstXI site of an appropriate vector, such as pNewBin4-HAPl-GFP (see Figure 1). Transformation The following procedure was used for transformation of plants 1. Stratification of WS-2 Seed. • Add 0.5 ml WS-2 (CS2360) seed to 50 ml of 0.2% Phytagar in a 50 ml Coming tube and vortex until seeds and Phytagar form a homogenous mixture. • Cover tube with foil and stratify at 4°C for 3 days. 2. Preparation of Seed Mixture. • Obtain stratified seed from cooler. • Add seed mixture to a 1000 ml beaker. • Add an additional 950 ml of 0.2% Phytagar and mix to homogenize. 3. Preparation of Soil Mixture. • Mix 24 L SunsbineMix #5 soil with 16 L Therm-O-Rock vermiculite in cement mixer to make a 60:40 soil mixture. • Amend soil mixture by adding 2 Tbsp Marathon and 3 Tbsp Osmocote and mix contents thoroughly. • Add 1 Tbsp Peters fertilizer to 3 gallons of water and add to soil mixture and mix thoroughly. • Fill 4-inch pots with soil mixture and round the surface to create a slight dome. • Cover pots with 8-inch squares of nylon netting and fasten using rubber bands. • Place 14 4-inch pots into each no-hole utility flat. 4. Planting. • Using a 60 ml syringe, aspirate 35 ml of the seed mixture. • Exude 25 drops of the seed mixture onto each pot. • Repeat until all pots have been seeded. • Place flats on greenhouse bench, cover flat with clear propagation domes, place 55% shade cloth on top of flats and subirrigate by adding 1 inch of water to bottom of each flat. 5. Plant Maintenance. • 3 to 4 days after planting, remove clear lids and shade cloth. • Subirrigate flats with water as needed. • After 7-10 days, thin pots to 20 plants per pot using forceps. • After 2 weeks, subirrigate all plants with Peters fertilizer at a rate of 1 Tsp per gallon water. • When bolts are about 5-10 cm long, clip them between the first node and the base of stem to induce secondary bolts. • 6 to 7 days after clipping, perform dipping infiltration. 6. Preparation of Agrobacterium. • Add 150 ml fresh YEB to 250 ml centrifuge bottles and cap each with a foam plug (Identi-Plug). • Autoclave for 40 min at 12 PC. • After cooling to room temperature, uncap and add 0.1 ml each of carbenicillin, spectinomycin and rifampicin stock solutions to each culture vessel. • Obtain Agrobacterium starter block (96-well block with Agrobacterium cultures grown to an OD600 of approximately 1.0) and inoculate one culture vessel per construct by transferring 1 ml from appropriate well in the starter block. • Cap culture vessels and place on Lab-Line incubator shaker set at 27°C and 250 RPM. • Remove after Agrobacterium cultures reach an OD600 of approximately 1.0 (about 24 hours), cap culture vessels with plastic caps, place in Sorvall SLA 1500 rotor and centrifuge at 8000 RPM for 8 min at 4°C. • Pour out supernatant and put bottles on ice until ready to use. • Add 200 ml Infiltration Media (IM) to each bottle, resuspend Agrobacterium pellets and store on ice.

7. Dipping Infiltration. • Pour resuspended Agrobacterium into 16 oz polypropylene containers. • Invert 4-inch pots and submerge the aerial portion of the plants into the Agrobacterium suspension and let stand for 5 min. • Pour out Agrobacterium suspension into waste bucket while keeping polypropylene container in place and return the plants to the upright position. • Place 10 covered pots per flat. • Fill each flat with 1 -inch of water and cover with shade cloth. • Keep covered for 24 hr and then remove shade cloth and polypropylene containers. • Resume normal plant maintenance. • When plants have finished flowering cover each pot with a ciber plant sleeve. • After plants are completely dry, collect seed and place into 2.0 ml micro tubes and store in 100-place cryogenic boxes. RECIPES: 0.2% Phytagar 2 g Phytagar 1 L nanopure water Shake until Phytagar suspended Autoclave 20 min YEB (for 1 L) 5 g extract of meat 5 g Bacto peptone 1 g yeast extract 5 g sucrose 0.24 g magnesium sulfate While stirring, add ingredients, in order, to 900 ml nanopure water When dissolved, adjust pH to 7.2 Fill to 1 L with nanopure water Autoclave 35 min

Infiltration Medium (IM) (for 1 L) 2.2 g MS salts 50 g sucrose 5 ul BAP solution (stock is 2 mg/ml) While stirring, add ingredients in order listed to 900 ml nanopure water When dissolved, adjust pH to 5.8. Volume up to 1 L with nanopure water. Add 0.02% Silwet L-77 just prior to resuspending Agrobacterium High Throughput Screening - Tl Generation

1. Soil Preparation. Wear gloves at all times. • In a large container, mix 60% autoclaved SunshineMix #5 with 40% vermiculite. • Add 2.5 Tbsp of Osmocote, and 2.5 Tbsp of 1% granular Marathon per 25 L of soil. • Mix thoroughly. 2. Fill Corn-Packs With Soil. • Loosely fill D601 Corn-Packs level to the rim with the prepared soil. • Place filled pot into utility flat with holes, within a no-hole utility flat. • Repeat as necessary for planting. One flat set should contain 6 pots. 3. Saturate Soil. • Evenly water all pots until the soil is saturated and water is collecting in the bottom of the flats. • After the soil is completely saturated, dump out the excess water. 4. Plant the Seed. 5. Stratify the Seeds. • After sowing the seed for all the flats, place them into a dark 4°C cooler. • Keep the flats in the cooler for 2 nights for WS seed. Other ecotypes may take longer. This cold treatment will help promote uniform germination of the seed. 6. Remove Flats From Cooler and Cover With Shade Cloth. (Shade cloth is only needed in the greenhouse) • After the appropriate time, remove the flats from the cooler and place onto growth racks or benches. • Cover the entire set of flats with 55% shade cloth. The cloth is necessary to cut down the light intensity during the delicate germination period. • The cloth and domes should remain on the flats until the cotyledons have fully expanded. This usually takes about 4-5 days under standard greenhouse conditions. 7. Remove 55% Shade Cloth and Propagation Domes. • After the cotyledons have fully expanded, remove both the 55% shade cloth and propagation domes. 8. Spray Plants With Finale Mixture. Wear gloves and protective clothing at all times.

• Prepare working Finale mixture by mixing 3 ml concentrated Finale in 48 oz of water in

the PoIy-TEK sprayer.

• Completely and evenly spray plants with a fine mist of the Finale mixture.

• Repeat Finale spraying every 3-4 days until only transformants remain. (Approximately 3

applications are necessary.)

• When satisfied that only transformants remain, discontinue Finale spraying.

9. Weed Out Excess Transformants.

Weed out excess transformants such that a maximum number of five plants per pot exist

evenly spaced throughout the pot.

GFP Assay

Tissues are dissected by eye or under magnification using INOX 5 grade forceps

and placed on a slide with water and coversliped. An attempt is made to record images of

observed expression patterns at earliest and latest stages of development of tissues listed

below. Specific tissues will be preceded with High (H), Medium (M), Low (L) designations.

Tl Mature: These are the Tl plants resulting from independent transformation

events. These are screened between stage 6.50-6.90 (means the plant is flowering and that

50-90% of the flowers that the plant will make have developed) which is 4-6 weeks of age.

At this stage the mature plant possesses flowers, siliques at all stages of development, and

fully expanded leaves. We do not generally differentiate between 6.50 and 6.90 in the report but rather just indicate 6.50. The plants are initially imaged under UV with a Leica Confocal microscope. This allows examination of the plants on a global level. If expression is present, they are imaged using scanning laser confocal micsrocopy. T2 Seedling: Progeny are collected from the Tl plants giving the same expression pattern and the progeny (T2) are sterilized and plated on agar-solidified medium containing M&S salts. In the event that there was no expression in the Tl plants, T2 seeds are planted from all lines. The seedlings are grown in Percival incubators under continuous light at 22°C for 10-12 days. Cotyledons, roots, hypocotyls, petioles, leaves, and the shoot meristem region of individual seedlings were screened until two seedlings were observed to have the same pattern. Generally found the same expression pattern was found in the first two seedlings. However, up to 6 seedlings were screened before "no expression pattern" was recorded. AU constructs are screened as T2 seedlings even if they did not have an expression pattern in the Tl generation. T2 Mature: The T2 mature plants were screened in a similar manner to the Tl plants. The T2 seeds were planted in the greenhouse, exposed to selection and at least one plant screened to confirm the Tl expression pattern. In instances where there were any subtle changes in expression, multiple plants were examined and the changes noted in the tables. T3 Seedling: This was done similar to the T2 seedlings except that only the plants for which we are trying to confirm the pattern are planted.

IMAGE DATA: Images are collected by scanning laser confocal microscopy. Scanned images are taken as 2- D optical sections or 3-D images generated by stacking the 2-D optical sections collected in series. All scanned images are saved as TIFF files by imaging software, edited in Adobe Photoshop, and labeled in Powerpoint specifying organ and specific expressing tissues.

Instrumentation: Microscope Inverted Leica DM IRB Fluorescence filter blocks: Blue excitation BP 450-490; long pass emission LP 515. Green excitation BP 515-560; long pass emission LP 590

Objectives HC PL FLUOTAR 5X/0.5 HCPL APO 10X/0.4 IMM water/glycerol/oil HCPL APO 20X/0.7 IMM water/glycerol/oil HCXL APO 63X/1.2 IMM water/glycerol/oil

Leica TCS SP2 confocal scanner Spectral range of detector optics 400-850nm. Variable computer controlled pinhole diameter. Optical zoom 1-32X. Four simultaneous detectors: Three channels for collection of fluorescence or reflected light. One channel for transmitted light detector. Laser sources: Blue Ar 458/5mW, 476nm/5mW, 488nm/20mW, 514nm/20mW. Green HeNe 543nm/l .2mW Red HeNe 633nm/10mW

RESULTS The section in Table 1 entitled "The spatial expression of the promoter-marker- vector" presents the results of the GFP assays as reported by their corresponding cDNA ID number, construct number and line number. Unlike the microarray results, which measure the difference in expression of the endogenous cDNA under various conditions, the GFP data gives the location of expression that is visible under the imaging parameters. Table 3 summarizes the results of the spatial expression results for each promoter.

EXPLANATION OF TABLE 1 Table 1 includes various information about each promoter or promoter control element of the invention including the nucleotide sequence, the spatial expression pattern associated with each promoter and the corresponding results from different expression experiments. TABLE l

I X in Sepal (Se), Receptacle (Re), Pedicel (Pd) in the upper part of the receptacle of the flower

T3 Seedling Expression Events Screened: Events Expressing: T3 seedling expression frequency: Plants expressing / Plants screened Event-01: 1/1 Event-02: 1/1 stronger expression seen in this line. D Scheduled D T3 Seedling tissue expressions similar to T2 seedling expression (data not shown) X T3 Seedling tissue expression (if different expression pattern than T2 seedling). GFP Expression Detected X Hypocotyl Depidermis H cortex Dvascular Dxylem Dphloem Dstomata □ Cotyledon Dmesophyll Dvascular Depidermis Dtrichome Dmargin Dstomata □ Rosette Leaf Dmesophyll Dvascular Depidermis Dtrichome Dpetiole Dprimordia

Expression is similar to original Two component line CS9107 (J1911). X in the junction of the Cotyledons (Co), the Cotyledons and the Hypocotyl (Hy) X in the vasculature of the root X in the Epidermis of the root tip

Promoter utility Trait-Subtrait Area: Among other uses this promoter sequence is useful to improve: PG&D- abscission, plant size Nutrients- nitrogen utilization Utility: Promoter useful in fruit abscission. Sinces expression overlaps the base of the gynoecium, it will be useful to overexpress genes thought to be important in supplying nutrients to the gynoecium or genes important in development of carpel primordia.

Construct: YPOOOl Promoter Candidate LD: 13148168 (Old ID: CS9107-1) cDNA LD: 12736079 Tl lines expressing (T2 seed): SR.00375-01,-02,-03,-04,-05

Sequence (SEQ ID NO: 1):

CTGCATTCACACATATTTTGGGCTCTCACGTGTTTGTGAATTTAATATATTTGACTACAC GATCTTTC AACGTATGAAAAAGTTTTATACTACTATTTTCGTTTGAGTGGGAAATAAACAAATGATAG CTACAG TTATCTATATGGTATAATTTTACACTTTTATAACTAATAATGATGAGTGATGACAATCGA GTGTCGG ATATAACAGGCCAACAAGTGGAATGGACTTATGTAACTTTTTAATCACGGGATTAAATCA CGTAAC CCAATGTCCTAATTGGTATTTAATTTTGATTATCTCGATGCTACATATTGTCATAGGACT CATATCTT TGATCACGTGCCGCTACCAATCCAGACATTTTAGTATACAAAAAAAAAGAAGATACAAAC TTAAG ATATGGAATATATATCAGAACTATCAGTTTTAGACTTTAATAATTCGAATTGAATAACTA CGATCA ATATATAAATTGGCAAATAGATTGGTCAATTGTAGTGCAAGAAATTTGTGAACTTTATTA CAGTAC GAAGAGAGTAAGAGAAGCAAGATCCGGTTTTTAGGCAACAAGTAACATTTTTGAGTTCAG AGAGT TTGCTTCTTACTTTAAGTTACGTCACTACAAAAGCCAAGTTCCTACTTCTTAGGTCTAAA GTCAATT TTCGAATATTCAGAAAAATTGTACTCTACTAGATCGAATAGTTTTCACCGGTGAAACGAT ATATAA ATGAAGACTACAATATTTTTTAATTTTTTTAAGCGTATGAGTTCTAGACCTTTGGCACGT AAATTTC TCCGGTACCTGGGACCAATCGTTGATAATATCACGTTTAAGATTTAATCATCCATCCCAA GTAGAG TTGAACTAGTAACCTTGAGCACTTTTTCTCGAGACAACTAAACCATCATCCACTTAGTGC AATAAA GCGTCATTCTTTTTTTTCTTTTCAAAAATTCGTATTTAATTTTAATTTATTAAAAATATT TCTTTTGTT TTAAATTGGGACAGAATTATCATTTAACATATTTAAAATTTATATTTTTAATTAAAAATA GGGTAAA ATATATTTTTCAAACAAAAATTCAAAAATAGGGCAATTTTCAAAATCATCCATTCTTAAA TCTAAA GTCGGCTACAGTCTTTTCGTTGTTTTGTTGCTAATTTCAATTTATATACATGCAAATTAC AAAATAT AATAGTTTTTGGGGGATAATTATCTTCTTGCGCCTTTTTATTAAATTAATATGCTCATAT AGCAGTT CTTACAATTAATATAACTAGGGTTTTAAATTTCAATATCGAGTTGACAAAATGAATTGTT TACAAGT TTTTTTCTTTTCAATATGCATTGTTCATCACGTATTCGTAGTGATGCAAAAACAAACTAT AAATTAT AATTGCACTAGTGAGATTAGCAAGAAGTGTTATAAATTAGAATAAACGGAACTATCAAAC TGTGTT ATGTACACCATTTATTTTTGTTAAAGAATATGTGTAGTAGTTAGAAAACTGATCAAATTA AACTGA AAATTCACATTACGGAGATCAAGTTACATTGTCTATTGATGAAAAAAACAAAATAAATCC AAATGG CACTAAAAGTTGTAGAAATTGAAAGAAGAAAATAGATTTTTGTCTAGGAATAAAAGTCAA AATGG GAAAGACAAAAAAAAGAGAGGCAAATAAGCAGTGATGGAGCTAAAGCAACGCTTTACTCT TTTAA TTATGAATTATTTGATTTGACCTCCACTCGCCTGGCTTTTTTTGGTTGTTCTTTATAGAA AAGTAAAA TAACACAATTAGCACATAACATGAGTTATCGAGAAACCAATTCTCTTTGTGGTGTTTTAG TTAATTT ■ CTATAACTTATGAAACCATTTTCTCAGTTTATCATGATAATTGATCCTCTATTTAAAACC CTAAAGT TTATATTTTGTTTGTTCAAACACAGTCGCCATTGCACTGGGATCCAACAATGTCCTCCGA CTCGTCC AAGATCAAGAGGAAGCGGAACCGCATnCCTTTAACGAAGGCGA

Promoter Expression Report # 2 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Ovule Pre-fertilization: (H)inner integument Post-fertilization: (M)seed coat, (M)endothelium Root (H)epidermis, (H)atrichoblast Cotyledons (L)epidermis Observed expression pattern: Tl mature: GFP expression exists in the inner integument of ovules. T2 seedling: Expression exists in root epidermal atrichoblast cells. Appended notes: T2 mature: Same expression exists as Tl mature. T3 seedlings: Same expression, plus additional weak epidermal expression was observed in cotyledons. Expected expression pattern: flower buds, ovules, mature flower, and silique Selection Criteria: Arabidopsis 2-component line CS9180(J2592). Gene: water channel-like protein" major intrinsic protein (MIP) family GenBank: NM 118469 Arabidopsis thaliana major intrinsic protein (MIP) family (At4g23400) mRNA, complete cds gi|3Q686182|reflNM 118469.2| [30686182] Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewBin4-HAPl-GFP Marker Type: X GFP-ER Generation Screened: X Tl Mature X T2 Seedling X T2 Mature X T3 Seedling

T2 Seedling Expression Tissues Screened Events Screened: n=5 Events Expressing: n=l

T2 Mature Plant Expression Plants expressing / Plants screened Event-01: Did not growEvent-02: 1/1 D Scheduled X T2 Mature tissue expressions similar to Tl expression data. DT2 Mature tissue expression (if different expression pattern). X in the Inner Integument (Ii) of developing ovules at approximately late heart stage embryo X in the Endothelium (Ed) which is derived from the inner integuments during the seed maturation stage.

T3 Seedling Expression Events Screened: n = 1 Events Expressing: n = 1 Seedlings expressing / Seedlings screened Event-01: Not screened Event-02: 1/1 D Scheduled DT3 Seedling tissue expressions similar to T2 seedling expression (data not shown). X T3 seedling tissue expression (if different expression pattern). Weak epidermal expression in cotyledons was not observed in T2 seedling screen.

Promoter utility Trait-subtrait Area: Among other uses this promoter sequence is useful to improve: Seed- seed size, seed enhancement Yield- total yield Water use efficiency- heat, water potential, drought, moisture stress Nutrients- nitrogen efficiency, phosphate efficiency Utility: Promoter could be used to misexpress any genes playing a role in seed size. It also has utility in misexpressing genes important in root hair initiation to try to get the plant to generate more or fewer root hairs to enhance nutrient utilization and drought tolerance. Notes: Pigment deposition occurring in the endothelium gives Arabidopsis seeds their characteristic brown color. Seed coat pigments in Arabidopsis are predominantly flavonoids or proanthocyanidins. Proanthocyanidins (PAs) accumulate as colorless compounds during the early steps of embryogenesis and have been shown to participate in the maintenance of seed coat imposed dormancy as well as in seed longevity during storage. Nesi, N, etal The Plant Cell, Vol. 14, 2463-2479,October 2002. Construct: YP0007 Promoter Candidate I.D: 13148318 (Old ID: CS9180-3 ) cDNA LD: 12703041 (Old LD: 12332468) Tl lines expressing (T2 seed): SR00408-01,-02,-05

Sequence (SEQ ID NO: 2):

AGCAGAACAACTATATTTATTGTGTCACATAAATCTGAGATCATTTATAACCACCAA AGAACCTAT ACACAGTAAATGACAAATGTATCTCCCTCTATCTCTATTGCCCATATGTAGATGCTAAAG TAAGATT TCTCTTTTTTTTAATGTACTTTTTTTTGTATAAAGTATATTCCATAAGAAAAAGGAAAAG CTTGTTTA TGGATCAATTGACCCCAAAAAAAGTTTTTAGATCAAAGCCCAATATAAAAAAAAAACACA GTAGT GACACAAAGGAACTTAAATAAACCATGAATTGATCTATAAACAGTAGAGATCGATAAGGC GAACA TTTTCCATGTGAAGTGTCTTCTTTCATCTATAATATTTTTGACATCCAATAATTTCCTCT ATAATATC ATTCACATAATTGATAGAAACATTATGTTAGAATTGTCCACATCATTTGAGCTGTAATAT ATTCTGT TTTAACAAATTATATGGTAGTTGCTTAATCTTATGTCCATCTTCTTCTATGCATCGTTTT CGCGCCTA GTTGTCCAGTCCATTTCAACTACCTACCTCTAATTCTTATCTTAAAACAACATTTTTTAA TTTAAGTA TTATGCTCAAAGACTAACTAGATAGAAAACCGTTATTAAACATTAAACGAATTAAAAGTC TTACAT GGAAAATGTAGGTTTATAAACCACGAGTTATGATTGACAATAAAAAAAATGCAAATCATC AATCA AAAGAGACTTGAGTGCGACTCTATATCAACCATTGCAATTAAAATTATCTATCACAAAAA TTTTAG ACAGATTAAGTTAATTTAGTCTAAATTCACTAATTTATTTTCTATAATTAGTAATTAACT ATATTTAT TTATTTACACATTTTCTGATAATTTAGAAATTTGCATGAATAACAAATATAAGATTTTGG AAATTAG TAGCAAATTTAATTAATAATTATTTTTGCCTAAATGAACCAAACTATAAAACCTCCACAT ACACCA GTCATCAAATTTACAGAGACAACAAACTAAAGTTGGTGGTGATAGAGTGAGAGAGAAACA CCATT GCACTGGGATCCAACAATGTCCTCCGACTCGTCCAAGATCAAGAGGAAGCGGAACCGCAT CCCG

Promoter Expression Report # 3 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Leaf (L)vascular Hypocotyl (L)epidermis Primary Root (H)epidermis, (H)cap Lateral root (H)epidermis, (H)cap Observed expression pattern: Tl mature: Low GFP expression was detected throughout the vasculature of leaves of mature plants. T2 seedling: No expression was detected in the vasculature of seedlings. Appended observations: T2 mature: Transformation events which expressed as Tl plants were screened as T2 plants and no expression was detected. This line was re-screened as Tl plants and leaf expression was not detected in 3 independent events. T3 seedling: New expression was observed in T3 seedlings which was not observed in T2 seedlings. Strong primary and lateral root tip expression and weak hypocotyl epidermal expression exists. Expected expression pattern: High in leaves. Low in tissues like roots or flowers Selection Criteria: Arabidopsis Public; SauerN. EMBQ J 1990 9:3045-3050 Gene: Glucose transporter (Sugar carrier) STPl GenBank: NM 100998Arabidopsis thaliana glucose transporter (Atlgl 1260) mRNA, complete cds, gij30682126MNM 100998.2|[30682126] Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewBin4-GFP Direct fusion construct Marker Type: X GFP-ER Generation Screened: X Tl Mature X T2 Seedling X T2 Mature XT3 Seedling

X in the Vascular (Vs) of the leaf

T 2 Mature Plant Expression Seedlings expressing/ Seedlings screened: Event-01: 0/4 Event-02: 0/2 D Scheduled D T2 Mature tissue expressions similar to Tl expression data (data not shown). D T2 Mature tissue expression (if different expression pattern). X No expression detected

T3 Seedling Expression Seedlings expressing/ Seedlings screened: Event-01 : 2/3 Event-02: 1/3 X Scheduled D T3 Seedling tissue expressions similar to T2 seedling expression (data not shown). X T2 Seedling tissue expression (if different expression pattern). NEW- Expression pattern not observed in previous generation- Expression detected

Promoter utility Trait-subtrait Area: Among other uses this promoter sequence could be useful to improve: Source- C/N partitioning, transport of amino acids, source enhancement Yield- Total yield Quality- Amino acids, carbohydrates, Optimize C3-C4 transition Utility: Sequence most useful to overexpress genes important in vascular maintenance and transport in and out of the phloem and xylem. Notes: Some weak vascular expression was observed in Tl that was not present in T2. The expression was also not observed in new Tl lines that were screened. The seedlings in the T3 do show some epidermal expression particularly in the root tip.

Construct: GOO 13 Promoter Candidate LD. : 1768610 (Old ID: 35139302) cDNA ID: 12679922 (Old IDs: 12328210, 4937586.) Tl lines expressing (T2 seed): G00013-01,-02,-03,-04,-05

Sequence (SEQ ID NO: 3): ATCTTGTGATACACAATTTATTACTATITGGTACATTITGAAGTATTTGTTTTTGCATGA TATATGACGTT AATTTGAACTGATATTAGTCAATTTATGCKJTACAAAAGTTGAAAGTTTAGAGCACTATG TTGGATTTATT AAAAATGATATCATACAATGGTTCAATATATATATATTTTTTTCCACGTTTTTAATAACA TγγTTGTAAAC AAGTCTTCTACTATTGTCTTTATTGTTAATGAGTTTCTAGTACCTAATTAGGAATTTTGA GGATATACGA^ ACATTAATGAGTTACATTATCCCGAAAACAAAATCTγGAAAACGAACAAAGATAATTTG GACATTACTC GTTATGTATACGTATGGAATTGGATAGAGCCGTTGAACCATCAAGTGGGTCTTCAAGTCA ACGAACTGA ATTTGATTTTACACTCATGTACATCGGCCACAATTTTATTCACACACTACTAACACCTCT GGTGTCCACTT TTTTCTTTCTCTAGATTGATGTGTTAAGATTTTTGTTGCAATTCATTTATTCAGGTATTT π ATATATAAATTAGAATAAACTAATTTAAAGAAAGATATAGCAATTATGTTTCACATTTTA ACATTCTCAA TCATTTATAAAACTAATGTGGTGATGAATGGTATATATATATATATATATATATATATAT ACATATATATA TTTTGTTGTGAACTAATGGTAAATATTTAAAATAAGACATACGTACATAAATCCACGGGC TCTTAAAGTC ATGATGCGGTTAATAAATGTTCACATAACGGTAACCAAGTGGCTCAAAATCATGAAACAA CGTCACATA ATTTATCTTATAATGTGGATAATTAGTACCGCATTATTTGTAAGAAAATTAAATTAATTA TAGATTCACA GCTAAGAAAATACGAAAAGACAGCTCAACACTTTTCCACTTCTATTCCCCACTGTCTATA TAACTCTGAT AAATAATCTCTGATCTCTCCACCATTGCACTGGTCCAGGAGATAAACAAGA

Promoter Expression Report # 4 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression Summary: Flower (H)sepal, (L)epidermis Embryo (H)suspensor, (H)preglobular, (H)globular, (M)heart, (M)torpedo, (L)late, (L)mature, (L)hypophysis Ovule Pre fertilization: (M)outer integument, (H)funiculus Post fertilization: (M)outer integument, (H)zygote Embryo (H)hypocotyl, (H)eρidermis, (H)cortex, (H)stipules, (L)lateral root, (H)initials, (EQlateral root cap Stem (L) epidermis Observed expression patterns: Tl Mature: Strong expression was seen in 4-cell through heart stage embryo with decreasing expression in the torpedo stage; preferential expression in the root and shoot meristems of the mature embryo. Strong expression was seen in the outer integument and funiculus of developing seed. T2 Seedling: Strong expression was seen in epidermal and cortical cells at the base of the hypocotyl. Strong expression was seen in stipules flanking rosette leaves. Low expression was seen in lateral root initials with increasing expression in the emerging lateral root cap. T2 Mature- Same expression patterns were seen as Tl mature plants with weaker outer integument expression in second event. Both lines show additional epidermal expression at the inflorescence meristem, pedicels and tips of sepals in developing flowers (T2 Mature Plant Expression). T3 seedling expression - same expression Expected expression pattern: Expression in ovules Selection Criteria: Greater than 5Ox up in pi ovule microarray Gene: Lipid transfer protein-like GenBank: NM 125323 Arabidopsis thaliana lipid transfer protein 3 (LTP 3) (At5g59320) mRNA, complete cds, gij30697205|reflNM 125323.2P0697205] Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewbin4-HAP 1 -GFP Marker Type: X GFP-ER Generation Screened: X Tl Mature X T2 Seedling X T2 Mature X T3 Seedling

X in the Embryo (Em) and Outer Integument (Oi) of the ovule X in the Mature Embryo

T3 Seedling Expression Seedlings expressing/ Seedlings screened: Event-01: 1/3 Event-02: 2/3 Same expression pattern observed as in previous generation. X Scheduled X T3 Seedling tissue expressions similar to T2 seedling expression (data not shown). □ T2 Mature tissue expression (if different expression pattern).

Promoter utility Trait-subtrait Area: Among other uses this promoter sequence is useful to improve: Water use efficiency- Moisture stress, water use efficiency, ovule/seed abortion Seed- test weight, seed size Yield- harvest index, total yield. Quality- amino acids, carbohydrate, protein total oil, total seed composition

Construct: YP0097 Promoter Candidate I.D: 11768657 (Old ID: 35139702) cDNA ID 12692181 (Old IDs: 12334169, 1021642) Tl lines expressing (T2 seed): SR00706-01,-02

Sequence (SEQ ID NO: 4): TTCATCTTTATATTTAAGAGTTTAAAAACTGCAACTTTTGTTTTTCTTTCACTAAGTCTT ATGGCCAC AGTTAATTAAAAGCAGATGAAAGGTGGTCCAATGGAAAAGGAGAATGTGATTGGGCTAGT TGGGA GAGTTCTGATGTCTAGTGTTGGGTACACGTGTCCGTCAGTTACACATAGCATTAAATCAG ACGGCA TGTCATTATTCAAATCTAGTTCACATAGTACGACTAATAGCTGATAAATTAATGATTATA CAGCAT ATGAATTATGAATTCAAAAAAAAAAAAAAATTGAAAATGTTAAGGAGATGCTATATTTTA CAAAA TTCATCGCAATGCTTTCTACTAATTTGCTAAGTGGTCTTCTCCAGTTAGTCTTGTCGATT CCAAGCG ATATTATTAAATCTTGAAGCATCGCTCAAAGCATTATAGCTTAAGATAACCAAATTGTTA TTAAAA ACACCTAGTGAAATTTTTAAATTAAAACAATTTTGATATCTTTGTAATATCTAATACTAC TCTTTCT GTGTCTAAAAGGATTAATTTTCAAAAATTTCACACATATTAAAAAAAAAAAAAAATTACT AGCTAA ACAATTTTCAATAATCATAAAACAATAGTAACTTAATAATTTTTTTTTATTTTCAAAATA GTCCTTC AAGTTTACAATTCATTTTAGTATTATAATCAACAAAATTTGTATTAAAAAGTTGGAAAAT TAATCTT TGTGGAACAAAAAAATCTAGAAATCATTTTTTAGAATTAGAGAGAGGTTTGATAAAAAAA AATAA AAAAAAATAGAGAGAGGTAGTACATACTAAACGATGTGATACTACTATTGACAAAATCTT AATTCT CAGTTTAGTAGAATAAACTAGAAGGAATGAATGAAGTAAATGCGAATCCAACTACTAACA AACCC TACTTAGTCATCATATTTTCCCATATGAAATCCCTATATAAACCCATCATCATCTCCCAC TTTTTTCA TATCCA

Promoter Expression Report # 5 Spatial expression summary: Ovule Pre-fertilization: (L)inner integument Post-fertilization: (H)inner integument, (M)endothelium Primary Root (H)endodermis Observed expression pattern: GFP is expressed in the endosperm of developing seeds and pericycle cells of seedling roots. GFP level rapidly increases following fertilization, through mature endosperm cellularization. GFP is also expressed in individual pericycle cells. Appended observations: Tl and T2 mature: Same expression pattern was observed in Tl and T2 mature plants. Closer examination of the images reveals that GFP is expressed in the endothelium of ovules which is derived from the inner most layer of the inner integuments. Lower levels of expression can be seen in the maturing seeds which is consistent with disintegration of the endothelium layer as the embryo enters maturity. T2 seedling: Expression appears to be localized to the endodermis which is the third cell layer of seedling root not pericycle as previously noted. T3 seedlings: Low germination. No expression was observed in the few surviving seedlings. Expected expression pattern: Expression in ovules Selection Criteria: Greater than 50x up in pi ovule microarray Gene: palmitoyl-protein thioesterase GenBank: NM 124106 Arabidopsis thaliana palmitoyl protein thioesterase precursor, putative (At5g47350) mRNA, complete cdsgil30695161|reflNM 124106.2|[30695161] Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewbin4-HAPl-GFP. Marker Type: (X) GFP-ER Generation Screened: (X) Tl Mature (X) T2 Seedling (X) T3 Mature (X) T3 Seedling Marker Intensity: (X) High D Med □ Low

T3 Seedling Expression Plants expressing / Plants screened Event-01: 0/3 Low germination: 3 of 9 seedlings Event-02: 0/1 Low germination: 1 of 10 seedlings X Scheduled D T3 Seedling tissue expressions similar to T2 seedling expression (data not shown). D T2 Mature tissue expression (if different expression pattern). No GFP Expression Detected

Promoter utility Trait - Sub-trait Area: Among other uses this promoter sequence is useful to improve: Seed - ovule/seed abortion, seed size, test weight, total seed Composition - amino acids, carbohydrate, protein to oil composition Utility: Promoter useful for increasing endosperm production or affecting compositional changes in the developing seed. Should also have utility in helping to control seed size. Notes: Endothelium is the location of pigment deposition, and it is this deposition that gives Arabidopsis seeds their characteristic brown color. Seed coat pigments in Arabidopsis are predominantly flavonoids or proanthocyanidins. Proanfhocyanidins (PAs) accumulate as colorless compounds during the early steps of embryogenesis and have been shown to participate in the maintenance of seed coat-imposed dormancy as well as in seed longevity during storage. Nesi, N, etal., Plant Cell, 14 2463-2479 2002.

Construct: YPOl I l Promoter Candidate I.D: 11768845 (Old ID: 4772159) cDNA ID 13619323 (Old IDs: 12396169, 4772159) Tl lines expressing (T2 seed): SR00690-01,-02

Sequence (SEQ ID NO: 2):

CGATTGGATTTAGTCTATACATTATAGGGCGCAAGTTTGTGGATTTAAGAATTATAT AAAAACTTGAAAT ATATAGTTTTTATGCATγCTCCγCITGTGTAATACATAAACCAAATATGAGATAGGTT AATCTGTATTTCA GATAATATTAAATTCCAAACAATATγITγACγTGTTATAAGAAGGCAATTAATATCT CTCTGTTAATGGCA AGTGGTACCAAGTAGTATTAAACTATTAATGCAATGGAAGAGTACTGTTGGAAATTATAA TCCTCTATCA CACATTCAAACAGATCTCCTGAAATCTTCTCTTCCAAACTTGTACTTCTCTGATCCAAAT GTAGGCTCCAA AATATAGACATTTACCATTTACTAAGTCCACAACTCCTTγCTTGTCTCCTTCAAAAATG ACTCTTGTGTAA CCACCATATGACTCCGACAGTTCGGCATTGCCATGATGAGAGCTTAAAAATTCACCTTCC TGAGCATTTC AAGTCITCACTCCCITAGCTTGACCTGAACCAAGATAAAATGCCTTTGTCGTCCCGTAAT ATCCATCCTGC TTTGGACGGCATCATAGTTACATTCGATCCATCCTATTTACAATGTTATTTTAGTATTAA AAACATGACAA TAAATTTGTTGTTAAACATATTCAAATACAATATGATTGGATTTATAAGTAATTGTAATA TGAAATGTCCT TAGTAATATGTTAAAAAATACATAGATACACACACGTACTAAAAGAGGCAACGCGGGAGA TGTCATTAG AGGAAGAACTAGGAAGCAGAGCGTTCATGCAAAATGCTACCAAAAACGTTAATGCAATAT CTCAACTAA TCAGCACAGTCCATTTCATACTGAGAATGTAAAAACCAATCAGCATCGTCCATTTTTTCA TCTAATTATTT GTTAACTCTTAATTGGCCACAACITCCAACCACATGACGCTCTTTCTATTCCCγγTAT ATATTCCCATCTCA AATGTTCTTGIGAGACACAAAATATCATAAACATATAAACATAAACGCCAATCGCAGCTT TTGTACTTTTG GCGGTTTACA

Promoter Expression Report # 6 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Stem (H)epidermis, (H)cortex Hypocotyl (H)epidermis, (H)cortex Silique (H)style, (H)carρel, (EQseptum, (H)epidermis Leaf (M)mesophyll, (M)epidermis Observed expression patterns: Strong GFP expression exists throughout stem epidermal and cortical cells in Tl mature plants. GFP expression exhibits polarity in T2 seedling epidermal cells. First, it appears in the upper part of the hypocotyl near cotyledonary petioles, increasing toward the root, and in the abaxial epidermal cells of the petiole. An optical section of the seedling reveals GFP expression in the cortical cells of the hypocotyl. T2 mature: Same expression pattern was seen as in Tl mature with extension of cortex and epidermal expression through to siliques. No expression was seen in placental tissues and ovules. Additional expression was observed in epidermis and mesophyll of cauline leaves. T3 seedling: Same as T2. Expected expression pattern: Expression in ovules Selection Criteria: Greater than 5Ox up in pi ovule microarray Gene: cytochrome P450 homolog GenBank: NM_ 104570 Arabidopsis thaliana cytochrome P450, putative (Atlg57750) mRNA, complete cds, gil30696174|reflNM...104570.2|[306961741 Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewbin4-HAPl-GFP Marker Type: X GFP-ER Generation Screened: X Tl Mature X T2 Seedling X T3 Mature X T3 Seedling

T2 Seedling Expression Tissues Screened Events Screened: n=2 Events Expressing: n=2 Seedlings expressing / Seedlings screened Event-01 : No data Event-02: No data GFP Expression Detected X Hypocotyl H epidermis H cortex Hvascular Dxylem Dphloem Dstomata D Cotyledon Dmesophyll Gvascular Depidermis Dtrichome D margin Dstomata D Rosette Leaf Dmesophyll Dvascular Depidermis Dtrichome Dpetiole Dprimordia Dstomata D stipule D margin

T3 Seedling Expression Plants expressing / Plants screened Event-01: 2/2 Event-02: 0/1 Low germination. D Scheduled X T3 Seedling tissue expressions similar to T2 seedling expression (data not shown). D T2 Mature tissue expression (if different expression pattern). GFP Expression Detected

Induction Screens I. ABA (10OuM) ABA X (low) in the hypocotyl of the YP0104 -01-02. 8Hr.H.CTRL T3 Seedling X (high) in the hypocotyl of the YP0104 -01-02. SHr.ABA T3 Seedling X (low) in the YP0104 -02-02.8hr ABA CTRL T3 Seedling X (high) in the YP0104 -02-02.8hr ABA T3 Seedling ABA:

X in the YP0104-01 IHr. CTRL X in the YP0104.02 6 hrs CTRL X in the YP0104.02 6 hrs- Rosette leaf guard cells

Promoter utility Trait - Sub-trait Area: Among other uses this promoter sequence is useful to improve: Water use efficiency - moisture stress, water use efficiency, ovule/seed abortion Seed - test weight, seed size Yield - harvest index, total yield Composition - amino acids, carbohydrate, protein total oil, total seed Utility: Useful when expression is predominantly desired in stems, in particular, the epidermis.

Construct: YP0104 Promoter Candidate ID: 11768842 cDNA ID: 13612879 (Old IDs: 12371683,1393104) Tl lines expressing (T2 seed): SR00644-01,-02,-03

5 Sequence (SEQ ID NO: 6):

TTTATTTTATTTTTTGAATGAAAATGTC TATGTCCCCAAITCGTCγGGCAACTCTCGTATATTAGTGAGAAAAATTTGTCCATTATT TACTGCACTATT ACCCTGTGTTAATTTTγγGTATTGAAATTGTTITTTTAGTAATTCACGTCATATAGCG AATGATTCT^ i o TTγAAAAATγCAGTCITAAGTTTACAAATTAAATAACGCTACTGTAACCAACTCTGTA CGACCAACATGT TCGAGTITITGTATATACGGCCATATATGTACATATTTTACTATAAAGCGAAAAAATCCA TAAATTATTTA ATTAATATATAAAGGTGCCATγCTATTTCCAATGTGCTTAGGAAAATGCAGAACCγCG TGCγATATCTCT GTCGCCACGTGCAAATATAACAATATGAAATAGAACTAGCAAATCTTGAAATCTAACTCT TAAGACTAA TTCAAGCACATACGTAGAGAAAGTTGACCAACGGTTATCAGCATTTTAACATGGACCTTA TCAACATTTT 15 AACAAAGTCCACAAACAACCAGTCTTACAATCGCATTGGTACAAGATAATCGAATTCATC TTCCATATAA CAAAACCTAAACCTTGGTGTGAAAAGGAGAAGATATGTATGTTAAAGGCCGCCTATGCCT CTGGTTTGG GGTATATGATTCTAAGATγAGGGTTTGAATATTTTCGTTAGCCTGCCATGAGATATATT TATGTGATAATT TAGAGCCTCTTATGCATTAATGCATAACCGACTAGATCCATGTGGTATTCAGCTAATCAG TACACACAAG ACAAAGTAGTAAATGAGTTTGATGAAGACTGTGGTCTGATAATTCCTATCAACGTTAAAT CTGTCGGGGC 0 CAGGCAGCCAGCAACATTTTGCCTACCAACGCTCTGAATTCAATTGAACCTAGGCTATAT AATAGCAGGC TAACTTAACTAAGAGTT

Promoter Expression Report # 7 Promoter Tested In: Arάbidopsis thaliana, WS ecotype Spatial expression summary: Flower (L)sepal, (L)ρetal, (L)silique, (L)vascular, (HQstomata, (L)pedicel Silique (L)vascular, (L)eρidermis Cotyledon (H)stomata, (L)root hair Observed expression patterns: GFP expressed in the vasculature and guard cells of sepals and pedicels in mature plants. GFP expressed in the guard cells of seedling cotyledons. T2 mature: Stronger expression extended into epidermal tissue of siliques in proximal-distal fashion. T3 seedling: Weak root hair expression was observed which was not observed in 12 seedlings; no guard cell expression observed. All epidermal tissue type expression was seen with the exception of weak vasculature in siliques. Expected expression pattern: Drought induced Selection Criteria: Expression data (cDNAChip),>10 fold induction under drought condition. Screened under non-induced condition. Gene: Unknown protein; At5g43750 GenBank: NM 123742 Arabidopsis thaliana expressed protein (At5g43750) mRNA, complete cds, gi|306943661reflNM_123742.2|[30694366] Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewbin4-HAPl-GFP Marker Type: X GFP-ER Generation Screened: X Tl Mature X T2 Seedling X T3 Mature X T3 Seedling

T2 Mature Plant Expression Plants expressing / Plants screened Event-01: 1/2 Event-02: 1/1 SAME PLUS- Original expression pattern was observed including new expression not observed in previous generation. D Scheduled

T3 Seedling Expression Plants expressing / Plants screened Event-01: 2/3 Event-02: 0/2 NEW- Expression pattern not observed in previous generation. D Scheduled D T3 Seedling tissue expressions similar to T2 seedling expression (data not shown) X T2 Mature tissue expression (if different expression pattern). GFP Expression Detected Weak expression in a few root hairs. No guard cell expression observed

Promoter utility Trait - Subtrait Area: Among other uses this promoter sequence is useful to improve: Water use efficiency - Heat Notes: The GFP expression pattern si similar to promoters that are 100% exonic.

Construct: YP0075 Promoter Candidate LD: 11768626 (0Id ID: 35139358) cDNA ID: 13612919 (Old IDs: 12694633, 5672796) Tl lines expressing (T2 seed): SR.00554-01,-02

Sequence (SEQ ID NO: 7):

TGGATTACAAATCATTAAGCTAATATCTTCGATGAATTAAGAAGATAAGTGGATAACAAG TACCTA ACCGCAATAGTCCATAAATTAAAACATTAATGTATTTGTCGTTGAAAATTTGGCCGACTT TTATTTG TTATTCTAGTTTCCACATCAAAAATGTTTGTACTTCGTAGCAATCCATCCACCTAAACCC CAAATCT TAATTTATATTTGTTGCGTTTAAATTTGGGTGAGATTTGATTCTAAGTAGTTGAGATAAA TTGATAT TCTATTCATTAGTAAAATGATAGAGAAATTGGTTTATAATAATTTTACCCTAGAACATGA CATGAT ATTGGTAACCATTAATCAAAGAAAGAGCAAAGCATTTAATTTACCCTACTCTCCAACCAC TCCAGC CTTTATTAGTTGCAGTTGGGAATCATTTCTTTATGATTCTTATGTCATTGTCTCCTAAAT CAATGAAG TGCCTTGACCTTGTTACTAATTCGAACATAGCAAAGCCAACTACATAGATCCTTTACAAA GTTCTAA AAACAGGTTGTTTAGGCGTCTAGACAAACAAAACCATTTTGTACGATTCAACAAATTGGT CCATAG AATGTTATTGATCTTTCTTGTTTAGGCATTCGATAAATCGGCTAATACATTATTTTTTTG TTTTGCTT TTTCCTTATTAAAAATATGCAAAGTATTATGATGTTTAACCTGAACTGAATTTTACATTT AACTGGA TATAGGAAAATATTGGGTTGAATTTAATAATTAAGCAATTGTCACGTAAATCAAATTGGG CTTAAT ATATATTGTTGATTTCAGCAAAGACAAAGTTGGGCCGTTTCAATAGTCTTCACGCGATGT AAGCGT TCACTAACCAACTAGAGAAGACAATCAAATGAATACGTTCCACGTGACGCTTACGAACTT GTCAGT CACTTTGGTAATATGACAGACAGTAACCAGTAAACTACTAATCTCTTTCGCTAACGAACA CACAAA A

Promoter Expression Report # 8 -ϊ. ~"% Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Flower (L) receptacle, (L) vascular Leaf (H)vascular, (H)epidermis Root (M)phloem Cotyledon (M)vascular, (M)hydathode Primary Root (L)epidermis, (M)vascular Observed expression patterns: Expression was seen at the receptacle and vasculature of immature flower and leaf, and phloem of seedling root. T2 mature: Similar to Tl expression. Strong expression was seen in vascular tissues on mature leaves. Vascular expression in flowers was not observed as in T1.T3 seedling: Similar to T2 seedling expression. Expected expression pattern: Vascular tissues; The SUC2 promoter directed expression of GUS activity with high specificity to the phloem of all green tissues of Arabidopsis such as rosette leaves, stems, and sepals. Selection Criteria: Arabidopsis public; Planta 1995; 196:564-70 Gene: "Sugar Transport" SUC2 GenBank: NM 102118 Arabidopsis thaliana sucrose transporter SUC2 (sucrose-proton transporter) (Atlg22710) mRNA, complete cds, gil306880041ref|NM 102118.2p06880041 Source Promoter Organism: Arabidopsis thaliana WS Vector: Newbin4-HAP1-GFP Marker Type: X GFP-ER Generation Screened: X Tl Mature X T2 Seedling X T3 Mature X T3 Seedling

T3 Seedling Expression Plants expressing / Plants screened Event-01: 2/2 Event-02: 1/2

Promoter utility Trait - Sub-trait Area: Among other uses this promoter sequence could be useful to improve: Source - Source enhancement, C/N partitioning Utility: Useful for loading and unloading phloem. Notes: Good vascular expression. 1: Gottwald JR, Krysan PJ. Young JC, Evert RF. Sussroan MR. Related Articles, Links t Genetic evidence for the in planta role of phloem-specific plasma membrane sucrose transporters. Proc Natl Acad Sci U S A. 2000 Dec 5;97(25): 13979-84. PMID: 11087840 [PubMed - indexed for MEDLINE]

H Truernit E Sauer N BsMtiArticies, Links z: s B The promoter of the Arabidopsis thaliana SUC2 sucrose-H+ symporter gene directs expression of beta-glucuronidase to the phloem: evidence for phloem loading and unloading by SUC2. Planta. 1995;196(3):564-70. PMID: 7647685 [PubMed - indexed for MEDLINE] r 3, Sauer N. Stolz J. Related Articles, Links

B SUCl and SUC2: two sucrose transporters from Arabidopsis thaliana; expression and characterization in baker's yeast and identification of the histidine-tagged protein. Plant J. 1994 Jul;6(l):67-77. PMID: 7920705 [PubMed - indexed for MEDLINE]

Construct: YP0016 Promoter Candidate LD: 11768612 (Old ID: 35139304) cDNA ID 13491988 (Old IDs: 6434453, 12340314) Tl lines expressing (T2 seed): SR00416-01,-02,-03,-04,-05

Sequence (SEQ ID NO: 8): AAACATGTTTTATGTAACTACTTTGCTTATGTGATTGCCTGAGGATACTATTATTCTCTG TCTTTATT CTCTTCACACCACATTTAAATAGTTTAAGAGCATATAAATTAATTATCTTCAAAAAGGTG ATTATAT GCATGCAAAATAGCACACCATTTATGTTTATATTTTCGAATTATTTAATACATTTCAATA TTTCATA AGTGTGATTTTTTTTTTTTTTGTCAATTTCATAAGTGTGATTTGTCATTTGTATTAAACA ATTGTATC GCGCAGTACAAATAAACAGTGGGAGAGGTGAAAATGCAGTTATAAAACTGTCCAATAATT TACTA ACACATTTAAATATCTAAAAAGAGTGTTTCAAAAAAAATTCTTTTGAAATAAGAAAAGTG ATAGAT ATTTTTACGCTTTCGTCTGAAAATAAAACAATAATAGTTTATTAGAAAAATGTTATCACC GAAAAT TATTCTAGTGCCACTCGCTCGGATCGAAATTCGAAAGTTATATTCTTTCTCTTTACCTAA TATAAAA ATCACAAGAAAAATCAATCCGAATATATCTATCAACATAGTATATGCCCTTACATATTGT TTCTGAC TTTTCTCTATCCGAATTTCTCGCTTCATGGTTTTTTTTTAACATATTCTCATTTAATTTT CATTACTAT TATATAACTAAAAGATGGAAATAAAATAAAGTGTCTTTGAGAATCGAACGTCCATATCAG TAAGA TAGTTTGTGTGAAGGTAAAATCTAAAAGATTTAAGTTCCAAAAACAGAAAATAATATATT ACGCTA AAAAAGAAGAAAATAATTAAATACAAAACAGAAAAAAATAATATACGACAGACACGTGTC ACGA AGATACCCTACGCTATAGACACAGCTCTGTTTTCTCTTTTCTATGCCTCAAGGCTCTCTT AACTTCAC TGTCTCCTCTTCGGATAATCCTATCCTTCTCTTCCTATAAATACCTCTCCACTCTTCCTC TTCCT

Promoter Expression Report # 9 |, φ Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Flower (L)inflorescence, (H)pedicel, (H)vascular Stem (L)phloem Leaf - (L)vascular Ovule Pre fertilization: (H)chalaza end of embryo sac Hypocotyl (M)vascular, (M)phloem Cotyledon (M)vascular, (M)phloem Root (H)vascular, (H)pericycle, (H)phloem Observed expression patterns: GFP expressed in the stem, pedicels and leaf vasculature of mature plants and in seedling hypocotyl, cotyledon, petiole, primary leaf and root. Expected expression pattern: Phloem of the stem, xylem-to-phloem transfer tissues, veins of supplying seeds, vascular strands of siliques and in funiculi. Also expressed in the vascular system of the cotyledons in developing seedlings. T2 mature: Same as Tl mature, no images were taken. T3 seedling: Same as T2 seedling, additional images were taken. Selection Criteria: Arabidopsis public PNAS 92, 12036-12040 (1995) Gene: AAP2 (X95623) GenBank: NM 120958 Arabidopsis thaliana amino acid permease 2 (AAP2) (At5g09220) mRNA, complete cds, gil30682579|ref1NM_120958.2|[30682579] Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewbin4-HAPl-GFP Marker Type: X GFP-ER Generation Screened: X Tl Mature X T2 Seedling X T3 Mature X T3 Seedling

Tl Mature Plant Expression Organs/Tissues screened Event Screened: n=3 Events Expressing: n=2 GFP Expression Detected X Flower L pedicel Dreceptacle Dnectary Dsepal Dpetal Dfilament Danther Dpollen Dcarpel Dstyle Dpapillae L vascular Depidermis Dstomata Dtrichome D Silique Gstigma Dstyle Dcarpel Dseptum Dplacentae Dtransmitting tissue D vascular Depidermis Dstomata D abscission zone X Ovule Pre-fertilization: Dinner integument D outer integument D embryo sac Dfuniculus H chalaza Dmicropyle Dgametophyte

T2 Mature Plant Expression Plants expressing / Plants screened Event-01 : 1/3 very weak line Event-02: 2/2 same as Tl X Scheduled X Completed X T2 Mature tissue expressions similar to Tl expression data (data not shown) see Table 1. D T2 Mature tissue expression (if different expression pattern). Expression detected

T3 Seedling Expression Plants expressing / Plants screened Event-01: 1/3 Event-02: 2/2 D scheduled X T3 Seedling tissue expressions similar to T2 seedling expression (additional image) D T2 Mature tissue expression (if different expression pattern). GFP Expression Detected X in the root vasculature of the mature seedling

Nitrogen Minus N CONTROL 2Hr -Low N; No expression.

6Hr -Low N X in the vasculature (Vs) of seedlings and seedling roots

2Hr- High N Expression observed 6Hr High N Expression observed

Promoter utility Trait - Sub-trati Area: Among other uses this promoter sequence could be useful to improve: Trait Area: Seed - Seed enhancement Source - transport amino acids Yield - harvest index, test weight, seed size, Quality - amino acids, carbohydrate, protein, total seed composition

Sequence (SEQ ID NO: 9):

TAAAGATCAGAAGAGGAAGGTTTCGCCGCGGCGGTTGCATCTTCACCGTCGATTTC ATCGTTACAGCGACGCCGGTAATTCCTAGGTTGCTTAGTTCCCATTCTCTCTCTAAA ATTAGGGCTCGAAATGAATTGTTGAACAAGATAGAGATCTTTTTCTGATCCCCGTCG AACATTTATTCAAGGCCAAAAAAAGCACACGGGAATTTAGAGTACCAATACATATCA AAACCTAATGGGCTTTGAATGGTTGCATGTGTGTGTTTATTTCTGATATGCAAAGCG ATCGATAGTCTTTTCCATACAAGTGTAAACTGTAAACAACGTAATTAAGCATAACAA TACAACTCTTTCTTCTCTTTTTTTTTGTAAACACAAAATAAAATTACATCAATTCATG CTTTTCCTAGTTCATCTGACATTTTCCAAAATTCATGTTCCATTGAGTCCCTAATACT TGTTCATATTCATATTAGGGTACATGAATAAAAGTTATCATTCTTGAAACTACTAAAT TTTCATAGTTTATTTTTCTTCTTTTCGTTTCACTTTCGAACAAAACACTACGCGTGGC ATTTGCAATGAATTCCACATTATATGGAATAACACCATGATGAACATTCTACATATA TAATTATTATGTTTAAGCACTTAGACAGCATAAATTCTTTCTAATTATATAAATCTAA CCTTGTTACATTGTACATCTATAAATTACTTGAAGAAATAACGAGTTCTATTTCTTTT TAAAAATTAAAAATACTATACCATATCTCAGTGATTAAGTTGAACCAAAAGGTACGG AGGAGAAACAAGCATTTGATTCTTCCTTATTTTATTTTATTCATCTCTCACTAATGAT GGTGGAGAAAAAAAGAAAATACCTAACAAACAAATATATATTGTCATACAAAAATAT TTCTATATTTTTAGTTAATTAGTTTATATTCCTCACTTTTCAGGGCTTATATAAGAAA GTGAGCAAACACAAATCAAAATGC

Promoter Expression Report # 10 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Flower (L)sepal, (L)pedicel, (L)vascular Silique (H)stomata Hypocotyl (M)epidermis Primary Leaf (H)stomata Root (H)epidermis, (H)root hairs Observed expression pattern: Tl mature: GFP expression was seen in the guard cells of pedicles and mature siliques. Weak expression was seen in floral vasculature.T2 seedling: Strong expression observed in epidermis and root hairs of seedling roots (not in lateral roots) and guard cells of primary leaves. T2 mature: Similar to Tl plants. T3 seedling: Similar to T2 seedling. Screened under non-induced conditions. Expected expression pattern: As described by literature. Expressed preferentially in the root, not in mature stems or leaves of adult plants (much like AGL 17); induced by KNO3 at 0.5 hr with max at 3.5 hr Selection Criteria: Arabidopsis Public; Science"279, 407-409 (1998) Gene: ANRl, putative nitrate inducible MADS-box protein; GenBank: NM 126990 Arabidopsis thaliana MAD S-box protein ANRl (At2gl4210) mRNA, complete cds gi|22325672[ref|NM 126990.2|[223256721 Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewbin4-HAPl-GFP Marker Type: X GFP-ER Generation Screened: X Tl Mature X T2 Seedling X T2 Mature X T3 Seedling

T2 Seedling Expression Tissues Screened Events Screened: n=2 Events Expressing: n=2

T2 Mature Plant Expression Plants expressing / Plants screened Event-01: 1/2 Event-02: 1/3 D Scheduled X T2 Mature tissue expressions similar to Tl expression data (data not shown). D T2 Mature tissue expression (if different expression pattern). Expression detected

T3 Seedling Expression Plants expressing / Plants screened Event-01: 1/3 Event-02: 2/3 D Scheduled X T3 Seedling tissue expressions similar to T2 seedling expression (data not shown). D T2 Mature tissue expression (if different expression pattern). GFP Expression Detected

Promoter utility Trait - Sub-trait Area: Among other uses this promoter sequence is useful to improve: Yield - Heterosis, general combining ability, specific combining ability Notes: Has GFP expression pattern of exonic promoter. References: H 3. Zhang H. Forde BG. Related Articles, Links An Arabidopsis MADS box gene that controls nutrient-induced changes in root architecture. Science. 1998 Jan 16;279(5349):407-9. PMID: 9430595 [PubMed - indexed for MEDLINE]

Construct: YP0033 Promoter Candidate LD: 13148205 (Old ID: 35139684) cDNA ID: 12370148 (Old IDs: 7088230, 12729537) Tl lines expressing (T2 seed) :SRXXXXX-01,

Sequence (SEQ ID NO: 10): GTCGATTGGAACTTCCAATTTCTAAACGGATGCAATAAGAACTTACATATTCTCTTTCAT TAGTCAT TTATTGGCCAGATTTATTAAAAAAAGTTTTACTCAATGACCAAGGATTAGAGTTAAAGAT AATATA GATTATTACATATATTATTCGAAAAAATATACCCATGTCCGACTTTTTAAACCTCAAAAA TATCAAA ACCAGAAAAGATGATACAACACAAAAAAACAATAAAATAATAAGTGGAAGAGATATCATC GGAC AACAGTACAAGTACAGCACCAGCTCTGCCAAAAGCCAAAACCATTTGTCAATTACAGAAA GATAC TAATGTTTGAAATTACTAAATTACCCCTCGGACTTTACAAAAGCATCTCTAACTTATCCA CGTGTCA GTCATCTATTGATTGTTTCAATACCACCTTGTATTAACGCCCCACGATTCGTGGTTGGGT ACACCTG ATAGTCCGAGGATATTTAAATCTCACGCGCTCGTGTCTATAATTCGACTGTACTCGCTTT TCTTGTC GTGATTTTAGCAATTTACGAAGTCAAATGTTTGACTCAATCAGACTTGCGCATAGGAGAG CGAGTA TAAATGTTTACTATACTCACGCAAGTGGGGCTTTATTGAAACTACTCTTTTGTAATAAAA CCAGCAG TGGTTTTGTTCTGAATCCGCTCTCTTGC

Promoter Expression Report # 11 ! %4 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Flower (H)epidermis, (H)sepal, (H)petal, (H)vascular Stem (L)vascular Hypocotyl (L)epidermis, (H)phloem Cotyledon (L)epideππis, (M)stomata, (L)vascular Root (H)phloem Observed expression pattern: Strong GFP expression was seen in the epidermal layer and vasculature of the sepals and petals of developing flowers in mature plants and seedlings. T2 mature: Expression was similar to Tl mature plants. Vascular expression in the stem was not observed in Tl mature. T3 Seedling: Same expression seen as T2 seedling expression Expected expression pattern: Predominantly expressed in the phloem. Selection Criteria: Arabidopsis public: Deeken, R. The Plant J.(2000) 23(2),285-290 Geiger, D. Plant Cell (2002) 14,1859-1868 Gene: potassium channel protein AKT3 GenBank: NM 118342 Arabidopsis thaliana potassium channel (K+ transporter 2)(AKT2) (At4g22200) mRNA, complete cds, gi|306857231ref]NM 118342.2|[30685723] Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewbin4-HAP 1 -GFP Marker Type: X GFP-ER Generation Screened: X Tl Mature X T2 Seedling X T3 Mature X T3 Seedling

X in the Sepal (Se) and Pedicel (Pd) of the flower X in the Epidermis (Ep) and Phloem (Ph) of the sepal X in the Epidermis (Ep) and Phloem (Ph) of the petal

T2 Mature Plant Expression Plants expressing / Plants screened Event-01: 1/1 Event-02: 1/1 D Scheduled X T2 Mature tissue expressions similar to Tl expression data . D T2 Mature tissue expression (if different expression pattern). Expression detected X Stem I Depidermis Dcortex L vascular Dxylem Dphloem Dpith Dstomata Dtrichome X in the Vasculature (Vs) of the stem

T3 Seedling Expression Plants expressing / Plants screened Event-01: 1/6 Weak line Event-02: 1/1 D Scheduled X T3 Seedling tissue expressions similar to T2 seedling expression (data not shown). D T2 Mature tissue expression (if different expression pattern). GFP Expression Detected

Promoter Utility Trait - Sub-trait Area: Among other uses this promoter sequence is useful to improve: Nutrient - Low nitrogen tolerance; Nitrogen use efficiency; Nitrogen utilization References: 1: Deeken R, Geiger D, Fromm J, Koroleva O, Ache P. Langenfeld-Hevser R, Related Articles, Sauer N, May ST, Hedrich R. UoM B Loss of the AKT2/3 potassium channel affects sugar loading into the phloem of Arabidopsis. Planta. 2002 Dec;216(2):334-44. Epub 2002 Sep 21. PMID: 12447548 [PubMed - indexed for MEDLINE]

2. Geiger D. Becker D. Lacombe B. Hedrich R. Related Artjtjjes.

Outer pore residues control the H(+) and K(+) sensitivity of the Arabidopsis potassium channel AKT3. Plant Cell. 2002 Aug; 14(8): 1859-68. Erratum in: Plant Cell 2002 Nov;14(l l):2975. PMID: 12172027 [PubMed - mdexed for MEDLINE]

Construct: YP0049 Promoter Candidate LD: 11768643 (Old ID: 6452796) cDNA ID 12660077 (Old IDs: 7095446, 6452796) Tl lines expressing (T2 seed): SR00548-01,-02,-03

Sequence (SEQ ID NO: 11):

TTCCAAATTCTTATGGTTCTCTAGTGTCATGATTTTGAGAATCACTCAACTCCAAAA ATATAATCCA CGATCCCGTGTTAATTATTGAAGAATCAATCGTTTTTAATTTCTCACCAATAGATGTTGC TCTTATT ACTTAAAACAAATTGTTCGGACAAATGTAGCAAGTGTGATACTTTGTGGGATCTTAAAGA CGATTT CTCCTATAACAGAGGACAAAACAGGTCGGTCAATTACAATGTCATCCCTCTTTGCCCTGT CTTTTTT TTTCTTCTTAAAACCTAACCATTTGATTGTTTCTAAAGGTATTTCAAGAATATATGATCA ATCTAGA TGAATACTATACCGACGATGACTACACACACAAGGAAATATATATATCAGCTTTCTTTTC ACCTAA AAGTGGTCCCGGTTTAGAATCTAATTCCTTTATCTCTCATTTTCTTCTGCTTCACATTCC CGCTAGTC AAATGTTAATAAGTGCACACAACGTTTTCTCGAAGCATTAGAATGTCCTCCTCTTAATTA ATCTCCT TCTGATTAGATTCTCAATAGAGTTTAAATTTGTTAATGGAGAGATATATTGGGACCCTCA AGGCTTC TAATTATACCACGTTTGGCATAATTCTCTATCGTTTGGGGCCACATCTTTCACACTTCAT TACCTTAT CACCAAAACATAAAATCAATCAACTTTTTTTTGCCTTATTGATTGTGTTGGATCCCTCCA AAATTAA AACTTGTGTTCCCCACAAAAGCTTACCCAATTTCACTTCAATCTTAACAAATAGGACCAC CACTACC ACGTACGGTTTGCATCATACAAACCACAAACTCCTTCTTCATTACAATTATTATATCATC TACTAAA ACCTCTTTCTCCCTCTCTCTTTCTTGTTCTTAGTGCTAAATTTTCTTTGTTCAGGAGAAA TATCCATT GCACTGGGATCCAACAATGTCATCCGACTCGTCCAAGATCAAGAGGAAAGCGG

Marker Type: X GFP-ER Generation Screened: X Tl Mature X T2 Seedling X T3 Mature X T3 Seedling

T2 Mature Plant Expression Plants expressing / Plants screened Event-01: 1/1 Event-02: 1/1 D Scheduled X T2 Mature tissue expressions similar to Tl expression data (data not shown). DT2 Mature tissue expression (if different expression pattern). Expression detected T3 Seedling Expression Plants expressing / Plants screened Event-01: 1/3 Event-02: 0/3 No expression D Scheduled X T3 Seedling tissue expressions similar to T2 seedling expression (data not shown). □ T2 Mature tissue expression (if different expression pattern). GFP Expression Detected

Promoter utility Trait Area: Among other uses this promoter sequence is useful to improve: Cold, PG&D, Sub-trait Area: Cold germination & vigor, plant size, growth rate, plant development

Construct: YP0060 Promoter Candidate I.D: 11768797 (Old ID: 35139885) cDNA ID: 13613553 (Old IDs: 4282588, 12421894) Tl lines expressing (T2 seed): SR.00552-02,-03

Sequence (SEQ ID NO: 12):

TGGAGCTTTATTGAAATGCAAGAAAGTAAACAAAGGAAGATCTTTAGATTGTCACCA AGAGTGGTCTGA AACTCTCATAACACTCAATCCTCCTCCTCCTCATCACCACCACTACAAAATATTATATTC TCTATCTCTCA ATCTATGAGGAGATGTATTCTATCAAGCATTTGAAATGATAAGAAACTGGCGATCATCCT CTACGTCACC ATCACTCCAAAATTATCCTCTTTCTAGGTTTAAGTTTTGTAATGATCGCCTTTATTTGTT GAGATCTCTAAC TTCTCGCATITCCAAAATGTTAAGTCCAATAACTGCATTGGTTAAGTTGGGGCGTTACTA GTCGGCTTAA ATCCAAATATCKJATTTGATTCCATATGTATGTGACAGTTTCTTAACGTTCATATTACAA TGAATGATGGAT CCTTGACTAGACAAAGAGAAAATGGATTGTCACTTCGTAGGAAAAATAGAAATTCTCCAC GAAGGCTGG TCTCCTTTATTTAACGACAAATTCACTCATAGTCTCATTCACAATTTGAACTTGTCTAAC ACAATGTGTTA TATACTCGCGAAAAGAAGCATAATAGGCTCTTAAGGGTAATCCACGAAACCAAAACACAT ATAAAACAT TAATATTTTTCTCTAAATITATTCATATCAATAATAAAGTTTACAAAAAATATAAAACAA TAATCCATACT TAGCCCATAGCTTCGTGTGGAAGAAGACTTGATTTTTGACTAGTCAACGAAAATGAGTAA ATGACGTATT CAGCTATAGTAAAAGGGATCATAAGCGGAAATTACAAAGAAGCTTTGAGGGTAAACTAGT CAAAAAGC ATAATCAGAAATAACTTAGGCCCAAAGCAAAAAGGAAAGGGCTCTGGATCCAGCCGCAAA TCAGAATCT GGTAAGTTCGAACGCCACGTCATCACCTAAATATCTGAAATATCTAATTAAGACTTGTCT ATATATAAAG GCTTCTCCTTTCACAATCCC

Promoter Expression Report # 13 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Ovule Post-fertilization: (H) endothelium, (H)micropyle, (H)chalaza Observed expression pattern: Tl and T2 mature: Strong expression was seen in the mature inner integument cell layer, endothelium, micropyle and chalaza ends of maturing ovules. Expression was not detected in earlier stage ovules. T2 and T3 seedling expression: None Expected expression pattern: Primarily in developing seeds Selection Criteria: Arabidopsis public; MoI. Gen. Genet. 244, 572-587 (1994) Gene: plasma membrane H(+)-ATPase isoform AHAl 0; GenBank: NM 101587 Arabidopsis thaliana ATPase 10, plasma membrane- type (proton pump 10) (proton-exporting ATPase), putative (Atlgl7260) mRNA, complete cds, gi|18394459| Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewbin4-HAPl-GFP. Marker Type: X GFP-ER Generation Screened: X Tl Mature X T2 Seedling X T3 Mature X T3 Seedling

T2 Mature Plant Expression Plants expressing / Plants screened Event-01: 1/1 Event-02: 1/1 D Scheduled X T2 Mature tissue expressions similar to Tl expression data (data not shown). D T2 Mature tissue expression (if different expression pattern). Expression detected T3 Seedling Expression Plants expressing / Plants screened Event-01: 0/3 Event-02: 0/3 D Scheduled X T3 Seedling tissue expressions similar to T2 seedling expression (data not shown). D T2 Mature tissue expression (if different expression pattern). GFP Expression Detected

Promoter utility Trait Area: Among other uses this promoter sequence could be useful to improve: Seed - Endosperm cell number and size, endosperm granule number/size, seed enhancement Yield - harvest index, test weight, seed size Quality - protein, total oil, total seed composition, composition Notes: Endothelium is the location of pigment deposition which gives Arabidopsis seeds their characteristic brown color. Seed coat pigments in Arabidopsis are predominantly flavonoids or proanthocyanidins. Proanthocyanidins (PAs) accumulate as colorless compounds during the early steps of embryogenesis and have been shown to participate in the maintenance of seed coat— imposed dormancy as well as in seed longevity during storage. Nesi, N, etal., The Plant Cell, Vol. 14, 2463- 2479,Oetober 2002. ; ____ References: 1: Harper JF, Mannev L, Sussman MR. Related Articles. Links Ij≡i The plasma membrane H(+)-ATPase gene family in Arabidopsis: genomic sequence of AHAlO which is Il I expressed primarily in developing seeds. MoI Gen Genet. 1994 Sep 28;244(6):572-87.

Construct: YP0092 Promoter Candidate I.D: 13148193 (Old ID: 35139598) cDNA ID 12661844 (Old ID: 4993117) Tl lines expressing (T2 seed): SR.00639-01,-02,-03

Sequence (SEQ ID NO: 13):

AAAGATTGAGTTGAGAGAGATGGTGGAGACGCAGAACAGACAAAGGGAGTTTACCATATA GTGCTCTA AAGGGCAATGAGATTGCAGTGATGTGGCTATCCGGGGAATCATCGCAGGTTATTCCTTCC CATGAGCAAC AATCAATGGATGGGTTCCAATTCAGAGGAGAAACAGAAGAAGAAACGTTTCCAGAGAACC ACAGTAGG GATTCRCGATCπGCGAGTTGCAGAGAGCCTCTGAAACTGCAATAGAAAGGACACTGATG AAAAGAACA CACTGAAGGAGTATGCCAATCATGTGAAAACTCAGAGCTTGTATTGGTCTTGTGGTTGAT GAAGTTCTCA CAAAACCTTTGGCTTTGAATCTCCCCTCATTAGTCATGGTGAGAACAAGAACAAGACGAG AAACAGACA AAGAAGATGAAAAAACTTGTTGGCCAGTGTTGACTAAGGGGGAATAGCCCCAGACATAAC AAAATTAG ACTTGTCGTACATCTTTAATATTTTTTTATCTGTTTCTTTGTCCTGACGCTTTCATTATT CCTGTGATCAATT TTCTCATACCATTGGTCCATCGITAATCCITTCTTAATTTCATTTTCTACGTAACATGAG AGGAGACCAAG TCCTATGAGAACAGTTGACGTAACAGTGGTTGTTAAGTTAAGTTAAAAAGAGGAAGCTAG TGAGAGTGA CCGTTAGGTAGAGAAGTGAGATCTTTAACCACTCγTCTTTCTCTCTCTCTCTGCTTTTT TCGTCGTCTTTCA CATCTACTGTTCGCAAACTCTCTTATGCTTCCAATAATGGTGATACCAATTGAGACTTGC AGGAGAATCT CCTCTTCTCCACACTCTATCAACTGGTCAGCCATGGAATGGTCGTTTCAGTTTCAATATT CCTGGATTCTTT TTAAGGATTCCTGTTTCTCITCTGTTCCTGGTATATTCTTAACGACGAAATTAGTATCGG ATCCTGGTAAT ACATγTTGAAGCTTTTAAGTACCATTGCACTGGGATCCAACAATGTCCTCCGACTCGTC CAAGATCAAGA GGAAGCGGAACCGCATCCC Promoter Expression Report # 14 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Flower (L)silique Silique (L)medial vasculature, (L)lateral vasculature Observed expression pattern: GFP expressed in the medial and lateral vasculature of pre-fertilized siliques. Expression was not detected in the older siliques or in T2 seedlings. T2 mature: Weak silique vasculature expression was seen in one of two events. T3 seedling: Same as T2 seedling. Expected expression pattern: Expression in ovules Selection Criteria: Greater than 5Ox up in pi ovule micro'array Gene: expressed protein; protein id: At4gl5750.1, hypothetical protein GenBank: NM 117666 Arabidopsis thaliana expressed protein (At4gl5750) mRNA, complete cds gi]18414516|ref|NM 117666.1|[18414516] Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewbin4-HAPl-GFP Marker Type: X GFP-ER Lines Screened: n=3 Lines Expressing: n=3 Generation Screened: X Tl Mature X T2 Seedling ' X T3 Mature X T3 Seedling

Tl Mature Plant Expression Organs/Tissues screened Events screened: n=3 Events expressing: n=3 GFP Expression Detected X Flower Dpedicel Dreceptacle Dnectary Dsepal Dpetal Dfϊlament Danther Dpollen Dcarpel Dstyle Dpapillae Dvascular Depidermis Dstomata Dtrichome L silique X Silique Dstigma Gstyle Dcarpel Dseptum Dplacentae Dtransmitting tissue L vascular Depidermis Dstomata Dabscission zone D Ovule Pre-fertilization: Dinner integument D outer integument D embryo sac Dfuniculus Dchalaza Dmicropyle Dgametophyte Post-fertilization: Dzygote Dseed coat Dprimordia Dchalaza Dmicropyle Dearly endosperm Dmature endosperm DEmbryo Dsuspensor Dpreglobular Dglobular Dheart Dtorpedo Dlate Dmature Dprovascular Dhypophysis Dradicle Dcotyledons Dhypocotyl DStem Depidermis Dcortex Dvascular Dxylem Dphloem Dpith Dstomata Dtrichome DLeaf Dpetiole Dmesophyll Dvascular Depidermis Dtrichome Dprimordia Dstomata Dstipule D margin X in the Lateral vasculature (Lv) and Medial vasculature (Mv) of the silique

T2 Seedling Expression Tissues Screened Events Screened: n=2 Events Expressing: n= 0 Seedlings expressing /Plants screened Event-01: 0/6 Event-02: 0/6 No GFP Expression Detected DHypocotyl □epidermis Dcortex Dvascular Dxylem Dphloem Dstomata D Cotyledon Dmesophyll D vascular □ epidermis Dtrichome Dmargin Dstomata DRosette Leaf Dmesophyll Dvascular Depidermis Dtrichome Dpetiole Dprimordia Dstomata Dstipule Dmargin DPrimary Root Depidermis Dtrichoblast Datrichoblast Dcortex Dendodermis

T2 Mature Plant Expression Plants expressing / Plants screened Event-01: 0/4 Event-02: 1/3 D Scheduled X T2 Mature tissue expressions similar to Tl expression data. □ T2 Mature tissue expression (if different expression pattern). Expression detected X in the Medial vasculature (Mv) of the silique

T3 Seedling Expression Plants expressing / Plants screened Event-01: 0/1 ; 1 of 12 seedlings germinated. Event-02: 0/12 □ Scheduled □ T3 Seedling tissue expressions similar to T2 seedling expression (data not shown). □ T2 Mature tissue expression (if different expression pattern). No GFP Expression Detected

Promoter utility Trait - Sub-trait Area: Among other uses this promoter sequence could be useful to improve: Water use efficiency - Moisture stress at seed set, Moisture stress at seed fill, water use efficiency, Ovule/seed abortion Seed - test weight, seed size Yield - harvest index, , total yield Quality - amino acids, carbohydrate, protein, total oil, total seed composition

Construct: YPOl 13 Promoter Candidate I.D: 13148162 (Old ID: 35139698) cDNA ID: 12332135 (0Id ID: 5663809) Tl lines expressing (T2 seed): SR00691-01.-03

Sequence (SEQ ID NO: 14):

TATGAAGAAATTATAATAGACTCTCATAAAAATAGTGTTACAACTTACATTCTCTTATAT AGAAATTAGG ATAAACAGAAATGTAAATAATATATTTCGAAATAATGTTAAATTTCCTAAATTCTAATAT TAATATTTAT AAATGGTCATTTAACTTTTTCGTACCGGTTCGATGGGACATGTGTTATATTCAGTTAAGG TTACCACCATG CGCCAACTTGGCCTCTACCAAGTCAACATGGATATGGACCTTATGGTTACATGCCGCCTC CGCCTCCACC GCTACCGGGATATGGATACAGAGGTCCGCCACCTCAGCAACCGACGAGGAATGAAACAAG GCAATAAT ATATTGATGCTATTGTGGATTTAGTTACTGATAATTAGTGCCTTAGTGACAGTTCAAAAA TGTTGTTCATC AATAATCTACAATTTAAGGTTTGTGTTGTGGAATGTTTCATGATTTTATGAAGTCTTGCT TATCAAAAAGT ATGATGATTAAGAATTTGACTTCATGGCATATTCATTTGAGTTAGCAAAACTTTTTTGTG TTGCACCπ AATTTATAAATTTATGATTTTTAACCATCGAAATTATATATTTGAAAAGACTATCTCTAC AAGCCAAACCC ACTGGGCCACCAATATGGGTTTATCTGCGAAATCTGTGAACCTTAGAAAATCAAAGCCCA TATCCACTTT GCTGGAACTTTGCTGGAATGTAGGTTAGACAAAACCTTAAGACGCAGCTACAAGTCTCTT ATGTGGCAG ATGTCAAAATTAATGAGCACGTATAATTTACCCAAGAGGAGCAAAATAAGATTAGCAGCT TAAATTAAT TGTGTTGGATTAAATGAAACTTGCACTATGAATGGCAAAAAAGAGGTTACAATCTAGCAA CCACCTCAT AAACCCTCATTAATGAGATACTGACγCGTGAACCAATCAAATCTCAAGTTTCGTAGTTT AAATAAGTAGT AAACACCγCCTGATCAAAGCATCACCACCACCAAATATCAAACGCAAAAACCTATTATC AAAAGAACTA CKAGAGGAAATGACTAATCCCCATGATCATGGTTATGCTGTTGTTGTTTCTTGTGATGTC GACTAGAGCAG ACGAAGACCTGATTAAGACAGAGTGTAATCACACAGAATACCAAAACGTATC^CTCTTCT GTCTTGAAG CCGATCCCAATCTCCTTCAATATCGGACCGTGCTGGACTTGTCAACCATCATTAATACAC TGTCTCGGGAT CTCAACTTGATGTTCTTATCAAGTAAGTTTCACCATGTACACCATTAATCATTATTGTAC AAATAATAATA TITTTAATGTGTTTTCACAAAATTAATATTACCTCITITTTGTAAACTAATATGCTACGA AAATTAACATA CTACGAAAAATGTGAATTAATATTA(CTN1GCCTGTAAATTTTTACCTCCATAATAATAT TAGCATACCACG AAAAAATGTAACGTATTTCTTTTGGTGTAATGTGAATTTTGCTACGGTAAACTTACγγ TACAAAGAAAGA AGAGCGTTTTCCAAGTGGAAAATAATACATTTTGCGGTTTATATATTATAGGAACGACTA TTGATTTGTTT TTTTGGTTGTACCATTGCACTGGGATCCAACAATGTCCTCCGACTCGTCCAAGATCAAGA GGAAGCGGAA CCGC

Promoter Expression Report # 15 |%w 3; Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Flower (L)silique Silique (L)medial vasculature, (L)lateral vasculature, (H)guard cells Rosette leaf (H)guard cell Observed expression pattern: GFP expressed in the medial and lateral vasculature of pre-fertilized siliques. Expression was not detected in older siliques. Guard cell expression was seen throughout pre-fertilized and fertilized siliques. T2 seedling: No expression was seen. T2 mature expression: Similar to Tl mature expression. T3 seedling: Guard cell expression not seen in T2 seedlings, however it is in the same tissue type observed in mature plants of previous generation. Expected expression pattern: Strong activity in the inner endosperm tissue of developing seeds and weak activity in root tips. Selection Criteria: Arabidopsis public; Plant MoI. Biol. 39, 149-159 (1999) Gene; Alanine aminotransferase, AIaAT GenBank: AAK92629 Putative alanine aminotransferase [Oryza sativa] gi|15217285|gblAAK92629.1]AC079633 9[15217285] Source Promoter Organism: Rice Vector: pNewbin4-HAP 1 -GFP. Marker Type: X GFP-ER Generation Screened: X Tl Mature X T2 Seedling X T3 Mature X T3 Seedling

X in the Guard cell (Gc), Lateral vasculature (Lv) and Medial vasculature (Mv) of the silique

T2 Mature Plant Expression Plants expressing / Plants screened Event-01: 1/1 Event-02: 1/2 D Scheduled X T2 Mature tissue expressions similar to Tl expression data (data not shown). D T2 Mature tissue expression (if different expression pattern). Expression detected

T3 Seedling Expression Plants expressing / Plants screened Event-01: 1/3 Event-02: 2/3 D Scheduled D T3 Seedling tissue expressions similar to T2 seedling expression (data not shown). X T2 Mature tissue expression (if different expression pattern). GFP Expression Detected

Promoter utility Trait Area: Among other uses this promoter sequence could be useful to improve: Seed, source, yield, quality Sub-trait Area: Seed enhancement, transport amino acids, harvest index, test weight, seed size, amino acids, carbohydrate, protein, total seed composition

Construct: YP0095 Promoter Candidate ID: 13148198 (Old ID: 35139658) cDNA ID: 6795099 in rice Tl lines expressing (T2 seed): SR00642-02,-03

Sequence (SEQ ID NO: 15):

TTCCTCGACCATGCCGTTGCCGGAACCGGCTAGCGCGGCCGGCCGGCGGCGGCGGGGAGG CCGCAGTGG GACGACGGGTGAAGGATCCTCCAGCTGCGGAAGGAGGTGGTCCTCGAGGCCGAAGGGGAG AGGCTACG GAGATGGAGGGAAGCCGAAGAGAAGGGAGGCTGCTGCTGCγGCTGCATTTGGGAGACGA GAACTCGAC TCGAGCCATGGCGGCAGATTGGTGTTTCACGGCGGAATGCTAACTAGATCCAGCATCTCC ATAGCAAAG GTAGAATGGTAGATTGAGGTGAGTTTTTTTTCCCCTCITCTGCAGTTTTGATGTATTATT ACTGCCCTCA^ TGATCγGGGTAACATATTTCTGAGCTCAGTAGAACγGTTAAAAAAAGGCAGAAATGCA CAAACTCTTCTC ACAAAACAACATACAAATGCITATATTTTGGACCGGAGGCAATACATGGTATATTTTTTA AAGTGAAAA AAACAATCAGACACATGGTATTGAGTGATAGCAAAGCTGGGTGACCACAGAAAATACCγ CCTGCTTTAA ATACITTATACCγGGGCTGTCAATCCTCGGAGTTCCTCCCAATGTAATGTCTGAGGAAG AAGTATTGCAG CγAAATTTTAAGGGTTTCITGTACGAAACAGGGACAATCAGAGATTAAGAAACTCTATG TGGAAAAGGC CATCCGCATTTTGTTATGTGATTCAACAAATAAGATGAGGAGGCAAAGTCATGGTTCTGT TCTAATTAAC AAATCγACγATGGGGGCCGTTGCTCCCTATTGTCCACGCTCCITTTCTTCATTTCTCT CCγGCAGGATATCT TGTCπTTGATTCTTCATTITAGGTCγTATAAATATCACGTGGTTCAGGCCTCCAATGT CAAATTATCATTA CGTGGAACTCTCTTAGATGCTTGAGAAAAGTTAGCTCTTACCTGTCCATAGAAGCTCCAA GGAAGCGAGA ATAGTAGATACTTTGGTTGGCC

Promoter Expression Report # 16 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Ovule Pre-fertilization: (M)gametophyte, (M)embryo sac Root (H)epidermis, (M)pericycle, (H)root hairs Lateral root (H)flanking cells Observed expression patterns: GFP expressed in the egg cell and synergid cell of female gametophyte in early ovule development. It expressed in polarizing embryo sac in later stages of pre-fertilized ovule development. No expression was seen in fertilized ovules. GFP expressed throughout the epidermal cells of seedling roots. It also expressed in flanking cells of lateral root primordia. T2 mature: Same as Tl mature. T3 seedling: Same as T2 seedling Expected expression pattern: Expression in ovules Selection Criteria: Greater than 5Ox up in pi ovule microarray Gene: Senescence-associated protein homolog GenBank: NM_119189 Arabidopsis thaliana senescence-associated protein family (At4g30430) mRNA, complete cds, gi|18417592|reflNM_119189.11[18417592] Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewbin4-H AP 1 -GFP Marker Type: X GFP-ER Generation Screened: XTl Mature X T2 Seedling X T3 Mature X T3 Seedling

T2 Mature Plant Expression Plants expressing / Plants screened Event-01: 1/1 Event-02: 1/1 0 Scheduled X T2 Mature tissue expressions similar to Tl expression data (data not shown). q T2 Mature tissue expression (if different expression pattern). Expression detected

T3 Seedling Expression Plants expressing / Plants screened Event-01: 2/3 Event-02: 1/3 α Scheduled X T3 Seedling tissue expressions similar to T2 seedling expression (data not shown). q T2 Mature tissue expression (if different expression pattern). GFP Expression Detected

Promoter utility Trait Area: Among other uses this promoter sequence could be useful to improve: Water use efficiency, seed, yield Sub-trait Area: Moisture stress, water use efficiency, ovule/seed abortion, harvest index, test weight, seed size, total yield, amino acids, carbohydrate, proteintotail oil, total seed composition

Construct: YPO 102 Promoter Candidate LD: 11768651 (Old ID: 35139696) cDNA ID: 13613954 (Old IDs: 12329268, 1382001) Tl lines expressing (T2 seed): SR00643-01.-02

Sequence (SEQ ID NO : 16 ) : ATTTGGTTGATAACGTTTTCACTCGACTAATTATATACTTCAGAAGGATAGTAATAGAAT ACCAAAATAATTAAA

ATTATGTACCGTTTTTTTCTCTGTTCTTTTGGCGATTTGGTTTAGTTCGTTACACTC TAAATGTTATTGCAGATA

TAAGAAAAAATATTCTTTGATATTCTTACAAAAATAAGTGTAAAACTTTTATTAGTC AAAATCTTCAATCTTTAA

GCCATAAAAGGTAATATACGAAATTTCTTCGCTAATCTCTTCACCTTCCTCTACGCG TTTCACTTTCACTTTATA AATCCAAATCTCCCTTCGAAAACAT

Promoter Expression Report # 17 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Ovule Pre-fertilization: (H)inner integument Post-fertilization: (H)inner integument, (M)outer integument, (M)seed coat Primary Root (L)root hair Observed expression pattern: GFP expressed in the inner integuments of pre-fertilized and fertilized ovules. Female gametophyte vacuole seen as dark oval. T2 mature: Same expression was seen as Tl with additional expression observed in similar tissue. GFP expressed in the outer integument and seed coat of developmg ovules and seed. T3 seedling expression: GFP expression was seen in a few root hairs Expected expression pattern: Expression in ovules Selection Criteria: Greater than 5Ox up va.pi ovule microarray Gene: putative protease inhibitor GenBank: NM 129447 Arabidopsis thaliana protease inhibitor -related (At2g38900) mRNA, complete cds, gi]30687699|ref|NM 129447.2j[30687699] Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewbin4-HAP 1-GFP Marker Type: X GFP-ER Generation Screened: X Tl Mature X T2 Seedling X T3 Mature X T3 Seedling

fertilized ovule X in the Inner integument (Ii) of the fertilized ovule

T2 Mature Plant Expression Plants expressing / Plants screened Event-01: 2/3 Event-02: 1/3 D Scheduled D T2 Mature tissue expressions similar to Tl expression data (data not shown). X T2 Mature tissue expression (if different expression pattern). Expression detected X Ovule Pre-fertilization: M inner integument D outer integument D embryo sac D funiculus Dchalaza Dmicropyle Dgametophyte Post-fertilization: Dzygote M inner integument M outer integument Dendothelium M seed coat Dprimordia Dchalaza Dmicropyle Dearly endosperm Dmature endosperm D embryo X in the outer integument(Oi) of the developing ovules X in the seed coat (Sc) of the developing seed

T3 Seedling Expression Plants expressing / Plants screened Event-01 : 2/2 Event-02: 0/0 No germination. D Scheduled D T3 Seedling tissue expressions similar to T2 seedling expression (data not shown). X T2 Mature tissue expression (if different expression pattern). GFP Expression Detected X Primary Root Depidermis Dtrichoblast Datrichoblast Dcortex Dendodermis Dvascular Dxylem Dphloem Dpericycle Dquiescent D columella D root cap L root hairs GFP expressed in a few root hairs. Promoter utility Trait Area: Among other uses this promoter sequence is useful to improve: Water use efficency, seed, yield Sub-trait Area: Moisture stress, water use efficiency, ovule/seed abortion, harvest index, test weight, seed size, total yield, amino acids, carbohydrate, proteintotail oil, total seed composition.

Construct: YPO 103 Promoter Candidate LD: 13148199(Old ID: 35139718) cDNA ID: 4905097 (Old ID: 12322121, 1387372) Tl lines expressing (T2 seed): SR00709-01,-02,-03

Sequence (SEQ ID NO : 17 ) :

GTTTTGAAGAACAATCTGGATCGAAATCTAACATAAGGTCATCGTATTCAAGTTACG CAGTCAAGG ACTTGACATCATCCTACTCTGGTCTGAGGTTACCACTTCCAAAGATGGGATTTTTCGACT CGGTATG CTTCCTAAGAAATTCGTTTTATTGAACCTAGCAAATATCTTGTAATGTAAGATTCCTGAG ATGATGA AGAAAAAACAAACTTTTGTTACAGCAGGAGAACGGAGAGAAAGAAAACAGAGAACCAAAT GCTC TTGAAGCAAACAGAAGAAGAAGACACAAATCCAAACTTGAGACTTCTTCTACACCAGAAA ACCGC AGCATTCTGGGACAACGCAAAACACGAAAGTGAAACGGGCAATGATATATATGTCTTGGG TGCGT TACAAGGCATCGTTTGCAACTGTTGAGTTGGATAAGTCAACTGTCTTCTTTTCCTTTGGT TGTAGTA GCTGCCTTTTTTTTCCTTTGTTGCTTTAAGAAATAGCCCGAAAAAAAGAATGTTCTACAT TTCGGAG CAGAAAACTAACCGAATGAGTTTTTGGTCGGATCATCGGATCGATCAGATATATTTTGAG TTACGA ACTGTTATAAAAAAAGCCATAATTTTGTGTTGAGTTTGCAAAATACCTTATAACTTGTTA TTTGAGA TTGCACCTCCATATATATTAATTCGTAAGAGTATTTATTAAGTAAGCTTTAGTATAAATC CTTTTTTC CTTTAAAGTAAGTTAATGTTCTACTAAATAATAGTAAAGTTGAAGAACCGCTCCGTTTTT ACACCAT GCACGTGTTATCTAACAAAGAAAATATGGTACACCTAATGGCTAATGCAAAGGACAACAC AATGA AACTAACTTGACTCTGTGTTATAGAAACCCATAGACATCTGCATACATCCTAGTATTTGT ATAAATT GGACTCAAATTCCTGAGGACAATCATAGCAAACAATCACATCATCGCAATATACATAAAC AAAAG AGGAAGAAAAA

Promoter Expression Report # 18 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Embryo (H)mature, (H)late Ovule (H) endothelium Primary root (L)root hair Observed expression pattern: Low levels of GFP expression were detected in late torpedo stage with highest levels in the mature and late embryo. High GFP expression was detected in late endosperm stage in endothelium layer of developing seed. T2 mature: Same as Tl mature. T3 seedling: GFP was detected in a few root hairs not observed in T2 seedlings. Expected expression pattern: Embryo and seed Selection Criteria: Arabidopsis public; Rossak.M. Plant MoL Bio.2001.46:717 Gene: fatty acid elongase 1; FAEl GenBank: NM 119617 Arabidopsis thaliana fatty acid elongase 1 (FAEl) (At4g34520) mRNA, complete cds, gi|30690063|ref|NM 119617.2|r306900631 Source Promoter Organism: Arabidopsis thalims. WS Vector: pNewbin4-HAP 1 -GFP Marker Type: X GFP-ER Generation Screened: X Tl Mature X T2 Seedling X T2 Mature X T3 Seedling

T2 Mature Plant Expression Plants expressing / Plants screened Event-01 : 2/3 Event-02: 0/3 No expression. D Scheduled X T2 Mature tissue expressions similar to Tl expression data. D T2 Mature tissue expression (if different expression pattern). Expression detected X first detected in late torpedo stage of embryo

Promoter utility Trait - Sub-trait Area: Among other uses this promoter sequence is useful to improve: Seed - Ovule/seed abortion, seed enhancement, seed size Yield Reference: 1: Rossak M1 Smith M, Kunst L. Related Articles. Links B Expression of the FAEl gene and FAEl promoter activity in developing seeds of Arabidopsis thaliana. Plant MoI Biol. 2001 Aug;46(6):717-25. PMID: 11575726 [PubMed - indexed for MEDLINE]

Construct: YPO 107 Promoter Candidate LD: 13148252 (Old ID: 35139824) cDNA ID: 12656458 (Old ID: 1815714) Tl lines expressing (T2 seed): SR00646-01,-02

Sequence (SEQ ID NO: 18):

TAACAATCCTTGGGAACATTGCATCCATAGATATCCGGTTAAGATCGATCTTTGAAC TCATAAAAA CTAGTAGATTGGTTGGTTGGTTTCCATGTACCAGAAGGCTTACCCTATTAGTTGAAAGTT GAAACTT TGTTCCCTACTCAATTCCTAGTTGTGTAAATGTATGTATATGTAATGTGTATAAAACGTA GTACTTA AATGACTAGGAGTGGTTCTTGAGACCGATGAGAGATGGGAGCAGAACTAAAGATGATGAC ATAAT TAAGAACGAATTTGAAAGGCTCTTAGGTTTGAATCCTATTCGAGAATGTTTTTGTCAAAG ATAGTG GCGATTTTGAACCAAAGAAAACATTTAAAAAATCAGTATCCGGTTACGTTCATGCAAATA GAAAGT GGTCTAGGATCTGATTGTAATTTTAGACTTAAAGAGTCTCTTAAGATTCAATCCTGGCTG TGTACAA AACTACAAATAATCTATTTTAGACTATTTGGGCCTTAACTAAACTTCCACTCCATTATTT ACTGAGG TTAGAGAATAGACTTGCGAATAAACACATTCCCCGAGAAATACTCATGATCCCATAATTA GTCGGA GGGTATGCCAATCAGATCTAAGAACACACATTCCCTCAAATTTTAATGCACATGTAATCA TAGTTT AGCACAATTCAAAAATAATGTAGTATTAAAGACAGAAATTTGTAGACTTTTTTTTGGCGT TAAAAG AAGACTAAGTTTATACGTACATTTTATTTTAAGTGGAAAACCGAAATTTTCCATCGAAAT ATATGA ATTTAGTATATATATTTCTGCAATGTACTATTTTGCTATTTTGGCAACTTTCAGTGGACT ACTACTTT ATTACAATGTGTATGGATGCATGAGTTTGAGTATACACATGTCTAAATGCATGCTTTGTA AAACGT AACGGACCACAAAAGAGGATCCATACAAATACATCTCATAGCTTCCTCCATTATTTTCCG ACACAA ACAGAGCA

Promoter Expression Report # 19 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Ovule Pre-fertilization: (M)gametophyte, (M)embryo sac Post-fertilization: (H)zygote Observed expression pattern: GFP expressed in the developing female gametophyte of unfertilized ovules and the degenerated synergid cell of the fertilized ovule hours after fertilization. No expression was observed in T2 seedlings. T2 mature: Similar expression as Tl mature. T3 seedling: Root expression in one of two events was not observed in T2 seedlings. No expression was observed in the second line which is consistent with T2 seedling expression. Expected expression pattern: Expression in ovules Selection Criteria: Greater than 5Ox up in pi ovule microarray Gene: Hypothetical protein GenBank: NM 112033 Arabidopsis thaliana expressed protein (At3gl 1990) niRNA, complete cds gi|l 8399438|reflNM 112033.1[1"18399438I Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewbin4-HAP 1 -GFP Marker Type: X GFP-ER Generation Screened: X Tl Mature X T2 Seedling X T3 Mature X T3 Seedling

T2 Mature Plant Expression Plants expressing / Plants screened Event-01: 1/1 Event-02: 1/1 □ Scheduled X T2 Mature tissue expressions similar to Tl expression data D T2 Mature tissue expression (if different expression pattern). D No expression detected

T3 Seedling Expression Plants expressing / Plants screened Event-01: 2/2* Event-02: 0/2 D Scheduled D T3 Seedling tissue expressions similar to T2 seedling expression (data not shown). X T2 Seedling tissue expression (if different expression pattern). GFP Expression Detected *Event 01: Root expression in one of two events not observed in T2 seedlings. No expression observed in second line which is consistent with T2 seedling expression. \

Promoter utility Trait Area: Among other uses this promoter sequence is useful to improve: Water use efficency, seed, yield Sub-trait Area: Moisture stress, water use efficiency, ovule/seed abortion, harvest index, test weight, seed size, total yield, amino acids, carbohydrate, proteintotail oil, total seed composition.

Construct: YPOI lO Promoter Candidate I.D: 13148212 (Old ID: 35139697) cDNA ID: 13604221 (Old IDs: 12395818, 4772042) Tl lines expressing (T2 seed): SR00689-02,-03

Sequence (SEQ ID NO: 19):

GGGATGCGGTTCCGCTTCCγCTTGATCTTGGACGAGTCGGAGGACATTGTTGGATC CCAGTGCAATGGTA ATATAAAACAAGAAAACAAGAGATTTTATAGGACAATCACTAAATGACATTTAATTGATT AAACATTTA TTCATTAATAATTGTATGTTACTAACITCAACATTTAATAATTTTGTTTAAGATACGTTT ACATCAGAGAC TATTAATATTTTTACAGGTTGTAACTTTAAACT^^ CTTAAAGATAACAACATTTCCGTTTTTTTTCAAATTATTACAAATCAAACTGATATATTA GACACAACAC GATTACACGTAATGAAAAAAGAAAAAGATAAAAAGATAAAAGAAGGGATCGATTCTGTTT GGTCTGGTT TAGTGAGATTCAAAGTTAAGCTCITCCTTTCAAGACATGCCITCγTAAACCGGGAATGT GAACGTTTGTA ATGTAGTCCGTCCAGTTAATGCTTCCAACATCAAATCCAAATTCTCTCTTCTCGTCCTCT GACATATTCTC CATTAATCTCTGGGGTATTGCTGTTATCAAATCTGTAAAAGAAACCAAAAAAAAAAGATG AAAACTTTG CGGGTACCCKJTγγTGTCTGCTCTAAGAATTAGAATGTTAATGAGTTCTGTCTTACCT TCCACCATAGAAAG TGTATGGCTCATAAATAGTAGCAAGGTGTTTGGCTTGTTCAACAGATγTCTTGCATATA AACTTγAGCTTC TGCATCATCTTACTATCCACTGAACTCATACCACTCATCAACCCACTCCGTTCTTGAGCA TCTCTCCACAA ATGATCCGAGAAATCATCAACGGAATTGAAAAGTTTCATCAAACGCACCATAATAGGATC ACCTTTAGA GTCCATGCATGGAGATGTTTTGTAGTGGTTATAAAGAAGCTCCGCTAAGTCTTCGAAAAC CAGCGGGTTT ATCGCCGAAGAAGCGATCTGATACACGTTTATTTCACKJTγCCGGATCTGACATTGCCA TTCCATGCγTTGC TATAGCAGCTAACGTTCC

Promoter Expression Report # 20 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Flower (L)silique Silique (M)medial vasculature, (M)lateral vasculature, (M)guard cells Observed expression pattern: GFP expressed in the medial and lateral vasculature of pre-fertilized silϊques. Expression was not detected in older siliques. Guard cell expression was seen throughout pre-fertilized and fertilized siliques. T2 Mature: Same as Tl Mature. T2 seedling: Same as T2 seedling. Expected expression pattern: Expression in ovules Selection Criteria: Greater than 5Ox up in pi ovule microarray Gene: hypothetical protein GenBank: NM 104488 Arabidopsis thaliana hypothetical protein (Atlg56100) mRNA, complete cds gi|18405686|reflNM 104438.1|ri8405686] Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewbin4-HAPl-GFP Marker Type: X GFP-ER Generation Screened: X Tl Mature X T2 Seedling X T2 Mature X T3 Seedling

T2 Mature Plant Expression Plants expressing / Plants screened Event-01: 1/2 Event-02: 1/1 D Scheduled X T2 Mature tissue expressions similar to Tl expression data (data not shown). D T2 Mature tissue expression (if different expression pattern). Expression detected

T3 Seedling Expression Plants expressing / Plants screened Event-01: 0/2 Event-02: 0/1 D Scheduled D T3 Seedling tissue expressions similar to T2 seedling expression (data not shown). D T2 Mature tissue expression (if different expression pattern). No GFP Expression Detected

Promoter utility Trait Area: Among other uses this promoter sequence is useful to improve: Water use efficiency, seed, yield Sub-trait Area: Moisture stress at seed set, moisture stress at seed fill, water use efficiency, ovule/seed abortion, harvest index, test weight, seed size, total yield, amino acids, carbohydrate, protein, total oil, total seed composition, composition

Construct: YPOl 12 Promoter Candidate LD: 13148226 (Old ID: 35139719) cDNA ID: 12321680 (Old ID: 5662775) Tl lines expressing (T2 seed): SR00710-01,-02,-03

Sequence (SEQ ID NO: 20);

TTATGTGCCCTGATGTCCγATGCAGATGGTGCAACTACTGCTTTTGGTGAGAAGCT TCGCGAACAAGTTG AGGAAAGGCTAGAATTTTATGACAAAGGTGTTGCCCCACGCAAGAACGTGGATGTAATGA AGGAGGTGA TAGAGAATCTAAAGCAAGGTATTTCTTGTAGCTGTTITTTTTTGGγγGTAATCAGAGT CCTCTTTATGATG CK^AAACTCAGTGTTTTTTTATCγGTTCCγCCTTγAGAAGAGGAAGGGAAGGAGCCA GTTGATGCCT^ GAAGAAAAGCAAGAAGAAGAAGGCAAAGGGTGAAGAAGAAGAAGAGGTGGTGGCAATGGA GGAGGA CAAGTCAGAGAAAAAGAAGAAGAAAGAGAAGAGGAAGATGGAGACTGCAGAGGAGAACGA GAAATCA GAGAAGAAGAAGACAAAGAAGAGTAAAGCTGGAGGAGAAGAGGAGACTGATGATGGTCAC AGCACCA AGAAGAAGAAGAAGAAGTCTAAGAGCGCTGAATAGAAAGGGATGCAACATTAACAAACCC TGTATTGT ATTTTTTTTTTGAGCTAAATTAATGTCGTCTGTTγITCGTAGTGAACATCGGAGAATTT I^ GAAACGATTCAAGGTTTGGCAATATCTγAAGTTTGTTTAGGTITγCACγATTTTGAC GTTTG^ AGGAGGCγCCTCCATTTTATAAAATACAATTACCAATTCCAGTGCTTTGCAAATGTTTC AATAATAGCTA AACTAACTACCAAATTGGAAAACTAGCTTAACAAGTTTGTGAAAATGAATTTGGAGCCAT ATGATTTATT ATTTTACCCAAATGGAGTAATAGAAGAAGAGCAGCTCGCGTTTGAATGGTCAGTTAACAT TAACAAAAG GTAAAATTGAATAGATGTTAAAACTTGTGTAAGTAAACAATAGAGCTACCTCCγγTTG AGAAGGATAGA TAAACTCGTGACCAACCACATTCCCAGTCCCATATTCTTAGTACAAATAAGAAATTCACA CCCCTCAAAA GAAATATAACATAATCAATCATAGGAAATATACTTCGCATAATGACGATAATGATCAAGT TTCTCCTGTT AGCTCTGCTCGTGATCTCTCCGATTTGCGCCGAGAAGGACCTGATGAAAGAGGAATGCCA TAATGCACA AGTTCCGACCATTTGCATGCAATGTCTTGAATCCGACCCAACCTCCGTTCATGCAGACCG TGTTGGCATC GCCGAGATCATCATACACTGTCTCGACTCTCGTCTCGATATCATCACCAAGCAAGTTTTC CCTTCTAATAA CCATACATATATATTAACTTAGATATATGACAATATTCTCTAACTAATATATCAATCTTT TTATTGTTCTA CCATTGCACTGGGATCCAACAATGTCCTCCGACTCGTCCAAGATCAAGAGGAAGCGGAAC CGC

Promoter Expression Report # 21 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Silique (H)stigma, (H)transmitting tissue Observed expression pattern: GFP expression was seen in the stigma and pollen transmitting tract spanning the entire silique. T2 seedlings: No expression. T2 Mature: Same as Tl. T3 seedlings: No data Expected expression pattern: Expression in ovules Selection Criteria: Greater than 5Ox up inpi ovule microarray Gene: putative drought induced protein GenBaπk: NM 105888 Arabidopsis thaliana drought induced protein - related (Atlg72290) mRNA, complete cds gi]18410044|ref|NM 105888.1|[18410044] Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewbin4-HAPl-GFP Marker Type: X GFP-ER Generation Screened: X Tl Mature X T2 Seedling X T3 Mature X T3 Seedling

T2 Mature Plant Expression Plants expressing / Plants screened Event-01: 1/1 Event-02: 1/1 D Scheduled X T2 Mature tissue expressions similar to Tl expression data (data not shown). D T2 Mature tissue expression (if different expression pattern). Expression detected

T3 Seedling Expression Seedlings expressing / Seedlings screened Event-01 : No data Event-02: D Scheduled D T3 Seedling tissue expressions similar to T2 seedling expression (data not shown). D T2 Mature tissue expression (if different expression pattern). GFP Expression Detected Utility: Among other uses, this promoter sequence is useful to improve: Water use efficiency - Moisture stress at seed set, Moisture stress at seed fill, water use efficiency, Ovule/seed abortion Construct: YPOl 16 Promoter Candidate I.D: 13148262 (Old ID: 35139699) cDNA ID: 12325134 (0Id ID: 6403538) Tl lines expressing (T2 seed): SR00693-02,-03

Sequence (SEQ ID NO: 21):

AAACGCCTCTTCGGTCCACGCTGTCGTTTTATTGAAGGAATTATATTTTATTTTAAT TGGGCCTGCA GGCTAAACTATAAGTCCGTCTGATATGGGTCGGGTTGGGCTTATGAGTTATGGGTCTGGT AGGGGT CAATTAGCTTAATTTCGATATGTGCCCTACTCTCGACCTAACGTTTTGAACACGTAAGAG AGAGTTT CTAATATTGAGTTGTCTAATTAACTCGATAGGCTTATACAAAGTGTTTCCGCATTTTACC TTCTTAA TAACTCATCATTCACTAACTAAGAAAAGTTTTACTCAGACCATATCTTCCGCTTCTTGAT TATTGTC AATTTGTTGTCACTCAATTTATCTCTTGCAAAATTTAGTTGAAATCATTTGGTTTCATCT TTGGCTCT TGAATAGTTGCATGTGTGTATTTAGTAAGTTCTTTTCAATTAAGAAGGAAGAATAAAACA AATTGT GGCCAGAAACAATTATGTTGAGTTTTATCTCATACGTTGGCTCACTCATCCCCATCTCTC TGCTTTT GAATCATTCTACTCCTCCCATTTTTTGATCGTCCTTTTTTCTGCTTCTGAACATGGATCA TTGTGCAT GTTCGGATGTTCCTCGATCGTGCTGAAACTCAAAGTCTGAATCGATTACCATAGACTCTC AACCCAT CTTTGATATATAAAAAAGAGCCTTAACCCATCTCTTCTACTCTCCCTCTCTAGAAACAAA CACATCA CGTGATGATCTGTTTCCCCCCATACTTACGGGATGATCAGAATGTGGCATGAGGAAAAAG CCAAGA AATAAGTTGATAAATTTAAGGTTTAATTTAACAAAAATGAGAGATTAATCTTTTCATTTT AGGGTC GCACGCGGTGTTTTGTGCAACCGCAGAAACTTCCTATAAATACCGATACAATGTGCATGC TTTCTA CAACTCAACTCACTCAAACCAAAAAAAGAAACATCAAACCCCAAAACACACATAACAATC ACAAA CCATTGCACTGGGATCCAACAATGTCCTCCGACTCGTCCAAGATCAAGAGGAAGCGGAAC CGCAnC CCGTTTGGAAATCAGnCCG

Promoter Expression Report # 22 Promoter Tested In: Arabidopsis ihaliana, WS ecotype Spatial expression summary: Flower (H)pedicle Silique (M)vascular Stem (H)cortex Ovule Pre-fertilization: (H)outer integument, (M)chalaza Hypocotyl (H)cortex Root (H)epidermis, (H)atrichoblast, (H)cortex Observed expression pattern: Strong GFP expression was seen in the adaxial surface of the pedicel and secondary inflorescence meristem internodes. High magnification reveals expression in 2-3 cell layers of the cortex. GFP expressed in the vasculature of silique, inner integuments, and chalazal region of ovule. Expression was highest in the outer integuments of pre-fertilized ovules decreasing to a few cells at the micropylar pole at maturity. Specific expression was in the chalazal bulb region where mineral deposits are thought to be accumulated for seed storage. GFP expressed in 2 cortical cell layers of the hypocotyl from root transition zone to apex. At the apex, GFP is expressed at the base of the leaf primordial and cotyledon. Root expression is specific to the epidermis and cortex. T2 Mature: Same as Tl mature. T3 seedling: Same expression in event 01 as in T2 seedlings. Event 02 expression is different with weak root epidermal, weak hypocotyl and stronger lateral root expression. This expression is variable within siblings in this family. Other seedlings were more like event 01. Expected expression pattern: Expressed in ovules and different parts of seeds Selection Criteria: Greater than 5Ox up in pi ovule microarray Gene: hypothetical protein T20K18.24 GenBank: NM 117358 Arabidopsis ihaliana expressed protein (At4gl2890) mRNA, complete cds gi|30682271|ref|NM 117358.2|[306822711 Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewbin4-HAPl-GFP Marker Type: X GFP-ER Generation Screened: X Tl Mature X T2 Seedling X T2 Mature X T3 Seedling

T2 Mature Plant Expression Plants expressing / Plants screened Event-01: 1/2 Event-02: 1/1 D Scheduled X T2 Mature tissue expressions similar to Tl expression data (data not shown). D T2 Mature tissue expression (if different expression pattern).

T3 Seedling Expression Plants expressing / Plants screened Event-01: 2/3 Event-02: 2/6 D Scheduled D T3 Seedling tissue expressions similar to T2 seedling expression (data not shown). X T2 Mature tissue expression (if different expression pattern). GFP Expression Detected YP0117.02.T3 seedling Event 01: weak lateral root, same as T2 seedling expression Event 02: stronger lateral root expression, weak epidermal expression, cortex, vascular expression. X in the Lateral root (Lr) of the hypocotyl root transition zone X in the lateral root primordia X in the Epidermis (Ep) and Vasculature (Vs) of the root

Promoter utility Trait - Sub-trait Area: Among other uses this promoter sequence is useful to improve: Water use efficiency - Moisture stress at seed set, Moisture stress at seed fill, water use efficiency, ovule/seed abortion Seed - harvest index, test weight, seed size Yield - total yield Quality - amino acids, carbohydrate, protein, total oil, total seed composition

Construct: YP0117 Promoter Candidate LD: 11768655 (Old ID: 35139700) cDNA LD: 13617054 (Old IDs: 12322571, 7074452) Tl lines expressing (T2 seed): SR00694-01,-02

Sequence (SEQ ID NO: 22):

GTCAGTGAGTCGATTGGATCACAGTCCTTTATGATAAAACAAACTCATAATTATTCC ACCGACAACATGC GTTTTAAATTATTTTTTCTTAAATTATATTATATTATATTGATATCAACCTAGCγAAAA TAATTCGGATGG CGAAATCGGACAATTγTTAATAGAAAAAATGGGTATGAAGATAGTCTATGATTCCGTTC TTAGCGACTAG AGGGACCTGCTCAAATCTCCCGGGTGATACGCGATGTCAAGCTCAATAGAACCCCACAAC CGACGAGAC CGAGAAATCCγTGATTTGGGCTAGAAGATTγTGAAATAAATTTAATATATTCTAAGTA ACTTGCTγA^ TTTTTTTCAAACTCTAAAGACATAACTAACATA^ AAATTAAACγAATGATTAGCTCTCTAACGTGTTγAATCγCGTATCAAGTTTTTT^ TATTAAAACATTGTACTATTGTTTCTATTTTGTTTAGCTATTATTCITGTGAAATGAA^ CAATTACγAAATGGCAATATTTATCTTGGAAAACTATACCTCTAATTGGATTAGGCCCT AGACATCCTCTT TAGCITATTGACGTTAAAATTATTCCCAAAACTATTAAAGTTTAGTAGTTTGAAAGATGC ATCAAGACCT ACTCAGATAGGTAAAAGTAGAAAACTACAGTTAGTGTGATTATATTTTAAAATATATAAA ACAATCTTAT TAAACTAAATATTCAAGATATATACγCAAATGGAAGATAAAAACATTTAGTCTGTTACC ACTACCAGCCT AGCγAGTCACγAATAGTCACITTGGAACTGAGTAGATATTγGCATCγTGAGTTACC ATGGACTCAAAAGT CCAAAAAGAGACCCCGAGTGAAAATGCγACCAACTTAATAACAAAGAAGCATTTACAGC GGTCAAAAA GTATCTATAAATGTTTACACAACAGTAGTCATAAGCACCATTGCACTGGGATCCAACAAT GTCCTCCGAC TCGTCCAAGATCAAGAGGAAGCGGAACCGCATCCCGTTAAACGAAGGCG

Promoter Expression Report # 23 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Flower (L)silique Silique (L)carpel, (L)vascular Observed expression pattern: Low levels of GFP expressed in the medial and lateral vasculature of developing pre-fertilized siliques. T2 mature: No Expression. T3 seedling: No Expression- Expected expression pattern: Expressed in ovules and different parts of seeds. Selection Criteria: Greater than 5Ox up in pi ovule microarray Gene: Putative vacuolar processing enzyme GenBank: NM 112912 Arabidopsis thaliana vacuolar processing enzyme/asparaginyl endopeptidase -related (At3g20210) mRNA, complete cds gil30685671|reflNM 112912.2[[3068567I] Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewbin4-HAPl-GFP Marker Type: X GFP-ER Generation Screened: X Tl Mature X T2 Seedling X T2 Mature X T3 Seedling

T2 Mature Plant Expression Plants expressing / Plants screened Event-01: 0/3 Event-02: 0/2 D Scheduled D T2 Mature tissue expressions similar to Tl expression data (data not shown). D T2 Mature tissue expression (if different expression pattern). No expression detected

T3 Seedling Expression Plants expressing / Plants screened Event-01: 0/3 Event-02: 0/3 D Scheduled D T3 Seedling tissue expressions similar to T2 seedling expression (data not shown. D T2 Mature tissue expression (if different expression pattern). No GFP Expression Detected

T3 Seedling Expression X Scheduled D T3 Seedling tissue expressions similar to T2 seedling expression (data not shown). D T2 Mature tissue expression (if different expression pattern).

Promoter utility Trait Area: Among other uses this promoter sequence is useful to improve: Water use efficiency - Moisture stress at seed set, Moisture stress at seed fill, water use efficiency, ovule/seed abortion Seed - harvest index, test weight, seed size Yield - total yield Quality - amino acids, carbohydrate, protein, total oil, total seed composition

Construct: YP0118 Promoter Candidate I.D: 11768691 (Old ID: 35139754) cDNA LD: 12329827 (Old ID: 4908806) Tl lines expressing (T2 seed): SR00711-01,-02,-03

Sequence (SEQ ID NO: 23):

AATTGAGAAAGGTGCCTCAATTTCAGTAGAACCTGACGCAAAATTTCGCGATCATGCATG ACTCAAATTG GTCTATCCACTCAAATAAAAAAGTCGTCTCCCTATCTAGTCGAAGTCCTCAATC TTTTTCTMTATTTATACTGGAATGAATT^^ AAAATTATAGCAATATTCATTAGTTAAATCATAATAATATTTGTTATTTCTGCTAAATAT ATTAGTTTTAA ATTGGTAAATATATCAGTCATCATACTTTATATATGTGCACAAGAAAAAGAGGAAAAAAA ACTAACγTTT AATAAATTGAACGCTATCCTCTATATCTCGTCCTGGTCCAAATGTAMCITCAATATCCTT ITGATTTTAT TGCTGATTGCTTTAAAAAATTTCACAAACACTTTTATCATTCTTTTATTCCACCAAAATC TACAGACATAA TACTITGTAATTTCATGTAAAAATCTTCAAAATTCGGGA AACTGGATTCATCTCCAAAGGTGTGGTGTCGTGTTCATATATGTGGAGTCGTCGGCTAGT AATATAATAA GGAAAAGAGTGAAACATATGTAGTATAACGTATTCCTAGTTTTTTCTCTCTGTATCAATG AATCACTAATC AAGTAGTATGCATTAATTGAATTATCAGAAGCTGGTCACAAAAGTCTACCAAAAAAAACA AAAAAATTG GTCGGAAGAAAATGAAAATAATGAGAATAAAAAAGGGAAAAAAAATAAGAAGCTAGCAAA CAAAGCA ATTAACATITCAAGGCAGTTAATTCATCATGCAAGGTGCITATGTGTGACAACGTCATGC GTTACTTTTTG CGTCTACACγCATCTCTCγAACGCAATCCACTAATTCγGGTAATGGATTCTGCTATT TAGACCAACCAGTT TCTTCGTCTCTCAATC

Promoter Expression Report # 24 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Flower sepal, petal, silique Silique epidermis Leaf mesophyll, vascular, epidermis, margin Hypocotyl epidermis Cotyledon mesophyll, vascular epidermis Observed expression pattern: Screened under non-induced conditions. Strong GFP expression was seen in epidermal and vasculature tissue of mature floral organs and leaves including photosynthetic cells. GFP is expressed in two cell layers of the margin and throughout mesophyll cells of mature leaf. GFP expressed in tine epidermal cells of hypocotyl and cotyledons and mesophyll cells. GFP expression in the leaf is non guard cell, epidermal specific. Expected expression pattern: N induced, source tissue- Selection Criteria: arabidopsis microarray-nitrogen Gene: hypothetical protein, auxin-induced protein-like GenBank: NM 120044 Arabidopsis thaliana auxin-induced (indole-3-acetic acid induced) protein, putative (At4g38840) mRNA, complete cds gi|l 8420319|ref|NM 120044.1|[18420319] Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewbin4-Hap 1 -GFP Marker Type: X GFP-ER Generation Screened: XTl Mature X T2 Seedling X T3 Mature X T3 Seedling

T2 Seedling Expression Tissues Screened GFP Expression Detected

T2 Mature Expression X Scheduled D T2 Mature tissue expressions similar to Tl expression data (data not shown). D T2 Mature tissue expression (if different expression pattern).

T3 Seedling Expression X Scheduled D T3 Seedling tissue expressions similar to T2' seedling expression (data not shown). D T2 Mature tissue expression (if different expression pattern).

Promoter utility Trait - Sub-trait Area: Among other uses this promoter sequence is useful to improve: Source - Photosynthetic efficiency Yield - seed size Additional notes: Expression seen in Epidermal bodies within epidermal cells thought to be involved in pathogen defense mechanisms.

Construct: YPO 126 Promoter Candidate LD: 11768662 (Old ID: 35139721) cDNAID: 12713856 (Old IDs: 12580379, 4767659) Tl lines expressing (T2 seed): SR00715-01.-02

Sequence (SEQ ID NO: 24):

CATTGTATCTGAGATGTGACTGTGAAGAACAAAGATTCATGACATGGTATTGTTAAGCCG CCCATGTGGA TGATCATAACCAAACTCTGTCCγCAGATTTACTCAACAGTGTGTGTGAAACAAAGGCTG GTTTAAGTATG AAACCGGCACCACATATCTCTTCTTCTTCTGATCATTCTCTCCTACATAGACCGCCATGA ATCCTCTTGGT GTCGACGATGATTCCCTγCGAATAATTTGCTTAGCACCCAAGAAACγCCTCAAAAAAG CCATATTTTCCC TTATGTTTTCCTGAAGCTTAAATGTTTCTTAGTCHTO^ TTAGTTTGTGAATCTAAAGGGGTTTAGTTACTTGGTATATAAACGAACGTATGAAAGAAA TGATTAAGG ATTTTTGAGGTTTTTCTTTTTAATTAC^^ CAATAAATGGTGTCTGAGAAGTTAGTGTCTAATGATGTCCTACATGATAACTTCATTGGG GCTTATTTGT CTCAAAGACATCACATGCCCAAATCTCTCTATAGATTATGTAGGGACATGAAGTTGTGTA CCTAATGAAC CACAAGTCTCTATCACTGATTAAGTCATACCTTCTTCTCAATGATATTCAAAAGACAGGA CCACATGATT TGATTATATACTGACAAAGTCACAAAAGCCITCAAAAAAATTCTGTGGCAAGAAAGGAAA ATTTGACTA GTTATAGTGTCTATCTAACAAACAAGTGGTCATATTGATTTCTATCTTCACATCAGAAAT CATGAAGATT GATCACTATACKJGCCCITACTγATCATGCCGTGGTCCGGCAAAGCCATGTGCTTGCTT GTTGGTGTAAAA ATTTATGAGCTGAAACTTTTGAAACCAATAAAGGGTTATCTACAAGTAATGTTCTTATCT ATATATACTC ATCACTGACTCCTTTCTGCTCTGCCATTGCACTGGGATCCAACAATGTCCTCCGACTCGT CCAAGATCAAG AGGAAGCGGAACCGCATCCCGCTC

Promoter Expression Report # 25 1I S- * Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Flower (H)sepal, (H)anther Silique (M)vascular Ovule Post-fertilization: (M)inner integument, (M)chalaza, (M)micropyle Stem (H)Pith Hypocotyl (H)phloem Cotyledon (M)epidermis Rosette Leaf (H)hydathode Primary Root (H)phloem, (H)pericycle Lateral root (H)phloem Observed expression pattern: Expressed in the vasculature of sepal and connective tissue of anthers in pre- fertilized flowers, inner integuments restricted to micropyle region, and chalazal bulb of post-fertilized ovules. GFP expressed throughout the phloem of hypocotyl and root and in pericycle cells in root differentiation zone. Screened under non-induced conditions. T2 mature: Same expression as observed in Tl mature. In addition, silique vascular expression was not observed in Tl mature.T3 seedling: Same expression as observed in T2 seedlings. In addition, expression was observed in cotyledon epidermal and rosette leaf hydathode secretory gland cells. Expected expression pattern: nitrogen induced Selection Criteria: Arabidopsis microarray Gene: probable auxin-induced protein GenBank: NM 119918 Arabidopsis thaliana lateral organ boundaries (LOB) domain family (At4g37540) mRNA, complete cds gi|18420067|reflNM 119918.11[18420067] Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewBin4-H AP 1 -GFP Marker Type: X GFP-ER Generation Screened: X Tl Mature X T2 Seedling X T2 Mature X T3 Seedling

D Leaf D petiole Dmesophyll D vascular D epidermis Dtrichome Dprimordia Dstomata D stipule D margin X in the inflorescence meristem X in ilie Vasculature (Vs) of the sepal X in the stamen X in the Micropyle (Mp), Funiculus (Fn), Inner integuement and Chalaza (Ch) of the ovule

T2 Mature Plant Expression Plants expressing / Plants screened Event-01: 1/1 Event-02: 1/1 D Scheduled D T2 Mature tissue expressions similar to Tl expression data (data not shown). X T2 Mature tissue expression (if different expression pattern). Expression detected X Silique Dstigma Dstyle Dcarpel Dseptum Dplacentae Dtransmitting tissue M vascular Depidermis Dstomata D abscission zone D ovule X in the Vasculature (Vs) of the silique

T3 Seedling Expression Plants expressing / Plants screened Event-01: 2/3 Event-02: 2/3 D Scheduled D T3 Seedling tissue expressions similar to T2 seedling expression (data not shown). X T2 Mature tissue expression (if different expression pattern). GFP Expression Detected X Cotyledon Dmesophyll Dvascular M epidermis Dmargin Dstomata Dhydafhode X Rosette Leaf Dmesophyll Dvascular Depidermis Dtrichome Dpetiole Dprimordia Dstomata D stipule Dmargin H hydathode X in the Epidermis (Ep) of the cotyledon X in the Hydathode (Hy) of the rosette leaf

Promoter utility Trait - Sub-trait Area: Among other uses this promoter sequence is useful to improve: Source - Photosynthetic efficiency Yield - seed size Notes: Hydathodes are modified parts of leaves; usually located at leaf margins or tips, in which water released from xylem is able to reach the surface of the leaf. It does so by passing through modified mesophyll and leaving through openings in the' epidermis. These openings are stomata that are incapable of opening and closing movememts.

Construct: YPO 127 Promoter Candidate LD: 13148197 (Old ID: 11768663) cDNA LD: 13617784 (Old IDs: 12712729, 4771741) Tl lines expressing (T2 seed): SR00716-01.-02

Sequence (SEQ ID NO: 25):

TCAGTGAGTCGATγGGAACGTITAAAGTTGAGACATAACGCAGTGATTTCAAATTT GTATTAGGGTGGTC TTATTGTGTGTCTAGCTACγAGCTAGAGAATACTAGAAGAAGAATACGTAGCAAGATAC GCATAACATTT GGTCCTCTCTTTTTTTTACTTTCTTTTAACACATTC^ TGTATCAATAATTCCCTCCAAATGATTAAACCCTAAAAAAATGTGATTCATTCACCACCC GAAGATTAGC ATCATCAAGTAACACACAATAACTACCAATAACCTAGTTTTCATTTTTCTATACTAAAAT CCTAAACATCC CATAAAAATACAAACAACTCTGAACCAATAATTTCCTCTAATCCACGTGCACCCCATCGT CTCCTGACGT AAGATTTGTCTATAACTTATCAAATCCCAAATTCAGCTTTGTTTTCATTATATAGTACGT ACTCTTATAAA AAAGAGAAGAGTACACATCTTTAATACTTTAACTTAAAAGAAGAAAGTAATACTAATATA AGAGGAGTC TGAGTCAGCGACAAGTGTTCGCGGAGAAACGGAAACGCTCTCTTTCTCTCTCTTCCCCCA ACGCCAATAC CITγGGAATCCCTCCCTAACTCTGTCCTGTCCπTγCGTCCTCACTTTCTCTCπTγ TTACATTTTCTACACACC AATAAAATTGAAACCAGCAACTTATAAATCAACTCAAGTTTGAATTAATGATCGAAAAAC TAGTTTATTT GTGTCAATATGACCCATTCTTTATTCACATAAGTATTTTAACTTTTCAAAATGTTATCTC AATCTCCTTTGA GTTTCTGTCTTCCCCATAATAAATTTCAAATAATTAATACACATGGTTTTTTAATTAGAA ATAATCRAAAAA GAAAGGACAAAGGAATAAAAAAGAAACACAAGTTGGCACACTCTCTTTATTATTCACTCC CCTCTATAA ATCTCATACTATCTTCTCTCATCTTCTT

Gene: similar to SP|P30986 reticuline oxidase precursor (Berberine- bridge-forming enzyme; Tetrahydroprotoberberine synthase) contains PFO 1565 FAD binding domain" product="FAD-linked oxidoreductase family" GenBank: NM 102808 Arabidopsis thaliana FAD-linked oxidoreductase family (Atlg30720) mRNA, complete cds gi|306920341ref|NM_102808.2|[30692034] Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewBin4-HAPl-GFP Marker Type: X GFP-ER Generation Screened: XTl Mature X T2 Seedling X T2 Mature X T3 Seedling

T2 Mature Plant Expression Plants expressing / Plants screened Event-01 : 0/4 Event-02: Not screened. D Scheduled D T2 Mature tissue expressions similar to Tl expression data (data not shown). D T2 Mature tissue expression (if different expression pattern). No GFP expression detected

T3 Seedling Expression Seedlings expressing / Seedlings screened Event-01: 1/2 Event-02: 2/2 D Scheduled D T3 Seedling tissue expressions similar to T2 seedling expression (data not shown). X T2 Mature tissue expression (if different expression pattern). GFP Expression Detected X Cotyledon Dmesophyll Dvascular D epidermis Dmargin Dstomata L hydathode X in the Epidermis (Ep) of the cotyledon X in the Epidermis (Ep) and Hydathode (Hy)

Promoter utility Trait - Sub-trait Area: Among other uses this promoter sequence is useful to improve: Water use efficiency - Heat Utility: This promoter is useful for root nutrient uptake. Additional notes: Hydathodes are modified parts of leaves , usually located at leaf margins or tips, in which water released, from xylem is able to reach the surface of the leaf. It does so by passing through modified mesophyll and leaving through openings in the epidermis. These openings are stomata that are incapable of opening and closing movememts.

Construct: YP0128 Promoter Candidate LD: 13148257 (Old ID: 11769664) cDNA LD: 13610584 (0Id IDs : 12327909, 4807730) Tl lines expressing (T2 seed): SR00717-01.-02

Sequence (SEQ ID NO: 26):

GATAAACTGATAATGGAAAAGAACAAAGAAACCAGTITγTAACTAITγGCATATGTAA TTTATTTGTTGC AAATTATATTTAGITAAAATGTTTCCTCTATTTATATATATATATATCAGTCAAGCACTA TGTATAAGAAA TGTCAATTTATAAATTTTTACATGTCGTTTAACAGAAAGAAAATGAATTTTTACATGTCA TTCATAGAGA GTCACγCGTITATTTCTTATATAGAGAATAACACACγCACATGCATATGCATGCAATA TGATACATTTTAT GACAAAGATAATCAACGGAAACGGTCAAGACATAATTTGATAAACAACTTGCACGATGCA CAGATCTGA TCAAATATATAACTCTTTAACATATCCAAAATATTCAAAAAGAAAAACTCGATCCAAACT AGCAACATCA CGCγCACGCGGTAGGCTAAAAATTTATTAATCTCCAAAAGTCTTTCTTATGAACACTGC AAACACAACAA CγTGAAAAGTCATATAGGTTTAGATGATGACGCGTATTGGCγATCGCTTACCGGAGTG GCTCATAAATAC AATAAACAATACGTAAAAGTCAAAGTCAAATATATTTAGTCAACTATAACCATTAATCGG GCAAAACCT TTAGCTGTCAAAACAACGTGAAAACGATATTTGTATATATCATCAAGAATCAGTAGATAA GAGAATGAT TTAATCCCCTGACTATTACAATTTTGGTGTAATAAA^ TCATGACCTATAGAGAGAATTAGGTAGTTTCGAAAATTGGCTAATCAACTTTTGAAAACT ACTGTCTACT TTGCγTAAATTCTCTACACγTAGTTTCGGATAAGATAATTGTCGGACTAATAGTTAAT CCCTTGACAATCT TTGATATTATAAAAGGTTTAGTTAATCTCITCTCTATATAAATATTCATACACCAGCTTT CAAAAATATAT AATCCAAACACCAAAAACAAA

Promoter Expression Report # 27 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Flower (L)stomata Silique (M)stomata Stem (L)stomata Cotyledon (L)mesoρhyll, (L)vascular, (M)hydathode Rosette Leaf (H)stomata, (H)hydathode Primary Root (L)root hairs Observed expression pattern: Expression specific to upper root hairs at hypocotyl root transition zone and hydathode secretory cells of the distal cotyledon. Tl mature: No Tl mature expression by old screening protocol. T2 mature: Guard cell and Hydathode expression same as Tl mature expression, T2 and T3 seedling expression. Note: Promoter lines up to YP0060 were screened for expression in the expected target tissue only. Expression patterns in these lines may be found coincidentally. YP0060 and later promoter lines were screened for expression in all aerial tissues. This line was re-screened in newly selected Tl mature plants. Expected expression pattern: Shoot and root meristem Selection Criteria: Literature. Plant Cell 1998 10 231-243 Gene: CYP90B1, Arabidopsis steroid 22-alpha-hydroxylase (DWF4) GenBank: NM 113917 Arabidopsis thaliana cytochrome p450, putative (At3g30180) mRNA, complete cds gi|30689806|ref]NM_113917.2|[30689806] LM 8/24/04 Cloned promoter not predicted sequence. NM 114926 DWF4 Arabidopsis thaliana steroid 22-alpha-hydroxylase (CYP90B1) (DWF4) (At3g50660) mRNA, complete cds Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewBin4-HAPl-GFP Marker Type: X GFP-ER Generation Screened: XTl Mature XT2 Seedling X T2 Mature X T3 Seedling

X Stem D epidermis D cortex D vascular Dxylem D phloem D pith L stomata Dtrichome D Leaf Dpetiole Dmesophyll Dvascular Oepidermis Dtrichome Dprimordia Dstomata D stipule D margin New Tl screen protocol. Weak guard cell expression in stems and flowers.

T2 Mature Plant Expression Plants expressing / Plants screened Event-01: 2/2 Event-02: 2/2 □ Scheduled X T2 Mature tissue expressions similar to Tl expression data □ T2 Mature tissue expression (if different expression pattern). Expression detected T2 mature expression same as Tl mature, T2 seedling, and T3 seedling X in the Guard cell (Gc) of the flower, silique and pedicle X in the Guard cell (Gc) and Vasculature (Vs) of the leaf

T3 Seedling Expression Seedlings expressing / Seedlings screened Event-01: 1/3 Event-02: 1/3 D Scheduled X T3 Seedling tissue expressions similar to T2 seedling expression (data not shown). □ T2 Mature tissue expression (if different expression pattern). GFP Expression Detected

Promoter utility Trait - Sub-trait Area: Among other uses, this promoter sequence is useful to improve: PG&D - Plant size, growth rate Utility: Useful to increase biomass, root mass, growth rate, seed set Additional notes: Hydathodes are modified parts of leaves, usually located at leaf margins or tips, in which water released from xylem is able to reach the surface of the leaf. It does so by passing through modified mesophyll and leaving through openings in the epidermis. These openings are stomata that are incapable of opening and closing movements.

Construct: YP0020 Promoter Candidate I.D: 11768639 (0Id ID: 11768639) cDNA LD: 12576899 (Old ID: 7104529) Tl lines expressing (T2 seed): SR00490-01,-02,-03,-04

10 Sequence (SEQ ID NO: 27):

CAGAGCAGTGCATATTTTTTTTTTTTTTTTTTTGGTGTTAGTGCATATCTATATATA TAGTACTATTA TAATATATTTCAATATATATATTTTAAGAAAATATCTGATTCTTAAGTTTGGACTTATTT GTCAACA 15 ATAGCCAGTAAAAAACAAAAGCGAAGTTTCACTAACTTAAAAAATAACCACATTTGTATA TTTCGA ATACATACTATAAATTAATAAATTTATCAAAACAACTATAGAAACTGTTATTTCCAATCA ATTTCTT TATCAAGATTATATCTGAAATATATTTATTAAAATTAATAGTTATTTACAAGAACTATTT TTATGAA AGTGTAAGAACTCTCTGAAAACTTGATAAGTCAATATTTTTTCTAACATCGTAAACATAA ACTAGA TTCAAATTCGAATCTAGTTATTCAAAAACTTATAAAAACATAAAAATGAAATACTGTTAC TTCAAC 20 AAAAAAACATTATTATTATTTTGTTTAAATATCTAATTTATTCATCAACAGCAAAATATT TAAAAGA GTGGGAAACAAATAAAAATTAAACTCTGTTTTGGTATGATAAAATTATTTACTAAACTAA ACTCAA TATATTTTTAGTATCACGGTTATAACTATAACAATAATCGAACTTTGTTATTTTCTTGGT ACTGGTTT TAGTAGTATAGATAGATATTTTAGTCATAACTCATAAGATACATGTACAAATATTTGCTA TATATG ATCAGTGATAACTGAATTTCGTGCTGAAAATTGCCATAGTTTGCTTATTTTACTCTTGAA ACAATAA 25 CGATATGGTCGTTACTTAAAACAACATTTTAAAAACGAAGAAAATTAAACAGAGTTTGTT AAAATA AATTAAATACCATAAATTTCTCTTTGACTCTTCCTATATAGTAAAATCTCTCATCCCCTT CTCTCTCT CTCTCATAGCATGTTGGTCTTTAGGTTCCTATATAAACAACGCCACACACACCCATTTAG TCCCACC ATTGCACTGGGATCCAACAATGTCCTCCGACTCGTCCAAGATCAAGAGGAAGCGGAACCG CATCCC GTTAAACGAAGGC 30

Promoter Expression Report # 28 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Flower (L)pedicel, (M)vascular Stem (H)vascular, (H)pith Silique (H)septum, (EQvascular Cotyledon (H)vascular, (H)epidermis Rosette Leaf (EQvascular, (H)phloem Primary Root (EQvascular; (H)phloem Lateral root (H)vascular Observed expression pattern: Tl mature (old protocol- screened target tissue): No expression observed. T2 seedling: Strong expression throughout phloem of hypocotyl, cotyledons, primary rosette leaves and roots. Also found in epidermal cells of upper root hairs at root transition zone. GFP expressed in a few epidermal cells of distal cotyledon. Tl mature: (new protocol-screened all tissues): High expression found in silique vasculature. T2 mature: Strong expression detected in inflorescence meristem and silique medial vasculature. T3 seedling: Same expression as T2 seedlings, however no cotyledon vascular expression was detected. Expected expression pattern: Shoot and root meristem Selection Criteria: Plant Physiol. 2002 129:1241-51 Gene: brassinosteroid-regulated protein (xyloglucan endotransglycosylase related protein GenBank: NM 117490 Arabidopsis tlialiana xyloglucan endotransglycosylase (XTR7) (At4gl4130) mRNA, complete cds gi|30682721|ref|NM 117490.2|[30682721] Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewBin4-HAPl-GFP Marker Type: X GFP-ER Generation Screened: X Tl Mature XT2 Seedling X T2 Mature X T3 Seedling

Tl Mature Plant Expression Organs/Tissues screened Events Screened: n=5 Events Expressing: n=0 (old protocol) No GFP Expression Detected Events Screened: n=3 Events Expressing: n=2 (new protocol) GFP Expression Detected □ Flower Dpedicel Greceptacle Dnectary Dsepal Dpetal Dfϊlament Danther Dpollen Dcarpel -D style D papillae D vascular D epidermis Dstomata Dtrichome .. - . , ■ - X Silique D stigma D style Dcarpel H septum Dplacentae D transmitting tissue H vascular D epidermis Dstomata D abscission zone ovule D Ovule Pre-fertilization: Dinner integument outer integument D embryo sac funiculus Dchalaza Dmicropyle Dgametophyte Post-fertilization: D zygote Dinner integument D outer integument funiculus Dchalaza Dmicropyle seed coat Dprimordia Dearly endosperm D mature endosperm D embryo D Embryo Dsuspensor Dpreglobular Dglobular Dheart Dtorpedo Dlate Dmature Dprovascular D hypophysis D radicle D cotyledons Dhypocotyl D Stem D epidermis D cortex D vascular Dxylem D phloem D pith Dstomata Dtrichome D Leaf Dpetiole Dmesophyll D vascular D epidermis Dtrichome Dprimordia Dstomata D stipule D margin X in the Vasculature (Vs) of the pre- fertilized and fertilized silique

X in the Epidermis (Ep), Phloem (Ph) and Root hair (Rh) of the hypocotyl root zone X in the Epidermis (Ep) and Vasculature (Vs) of the petiole leaf X in the Epidermis (Ep), Phloem (Ph) and Vascular bundle (Vb) of the Cotyledon (Co) X in the Phloem (Ph) of the Lateral root (Lr) and lateral root initial X in the Phloem (Ph) and Vascular bundle (Vb) of the Root (Rt)

T2 Mature Plant Expression Plants expressing /Plants screened Event-01: 1/2 Event-02: Not screened. X Scheduled D T2 Mature tissue expressions similar to Tl expression data. D T2 Mature tissue expression (if different expression pattern). X Flower L pedicel D receptacle D nectary 0 sepal D petal D filament D anther D pollen D carpel D style D papillae M vascular D epidermis Dstomata Dtrichome X Silique D stigma D style D carpel H septum Dplacentae D transmitting tissue H vascular D epidermis Dstomata D abscission zone ovule X Stem D epidermis D cortex H vascular Dxylem Dphloem H pith Dstomata Dtrichome X in the Vasculature (Vs) of the inflorescence meristem and silique X in the Guard cell (Gc) of the pedicle X in the Septum (Sp) of the silique ■

T3 Seedling Expression Seedlings expressing /Seedlings screened Event-01: 2/3 Event-02: 2/2 D Scheduled X T3 Seedling tissue expressions similar to T2 seedling expression (data not shown). D T2 Mature tissue expression (if different expression pattern).

Promoter utility Trait Area: Among other uses this promoter sequence is useful to improve: PG&D - Plant size, growth rate Utility: Useful to increase biomass, root mass, growth rate

Construct: YP0022 Promoter Candidate LD: 11768614 cDNA LD: 12711515 (Old ID: 5674312) Tl lines expressing (T2 seed): SR00492-02,-03

Sequence (SEQ ID NO: 28):

TAGTTCCATTACAATTTCCAAATGATTTGTTACAAAGCTACAAGATTATTCGAAATAGGA TTTCATC CATAAGAGAGAATGGTGTGGTCGACGCTACAATGTTGATTTATTGGTTGTGGTTTGCATC TTGGGG ATGTCAAATCCTAAGTTTCAAGTTCTTGTAAAAACGTTTTCAGGTTTCTTTAATATATTT TAATATTA ATGTAAAAAGAAAAGATATAGCTTTTGTACAAAAAAATTTGTTTAATCACTATGTAGGAG GATGCG ATCAAATTCATGGAATGATGTATTATTAGCTTTTCTATCCTCACTCTAAAAACAATACTA TAGTGAG TTAAATAATTTGATCATTTCAATGTAGATTAAAATTTTATTAAAAGAAGAAAAATTTAAA AGCCTA TAACAAAATAAAAAAGGAGGCTCGAGGTATGATGGGTGTAGCAGAAGAGCTGGCAACAGC TATCG ACTGAGTGATTACGAACTCAGTACTCAGTGTTCTCAGCTCACACACTCTTTTTTTGTTCT CTTTCTTT TGGACAGCTTTCATTTTCTCTTTTCTTTTTTCTATTTTGTTTCAAAATTCCATCCATATT AAAATAAG CCTGATCATGAGAATAAAGGAAATACTAATGATGAGTTTCTCAATAATGCAATAAGATGC AATTAT TATGAGCTATTTACTATTGAAAATGAGCAAATAAATGTCAAAACACAATCTGGTTAAGTT AGAGCA ACTCCATTGTATAGGATTCATGTAGTTTCTAAGAAAACAAAATGTATTAATATTTTACTT TTACATC CAAAAAACCAAAAAACCAACTTATATGAGTAATAGAAACGATCCTCATATTAGGAATTTT AGAGA TTTTCTCTCATCTGTTTCTTAACTTTTCAATATTTTTATTTTTTTAAATTGTATGAGTTT CTACTAAGA AACTACTGCTGGAGTTGGTCTTAGCTTCCCAATGCTTCTCCACCTATATATATGCATATC TCCTTCTT AAAACTCCATTGCACTGGGATCCAACAATGTCCTCCGACTCGTCCAAGATCAAGAGGAAG CGGAA CCGCATCCCTTATACTAAAGGCGG

T2 Seedling Expression Organs/Tissues Screened Events Screened: n=4 Events Expressing: n=2 Seedlings expressing / Seedlings screened

T2 Mature Plant Expression Plants expressing / Plants screened Event-01: 2/2 Event-02: 1/2 D Scheduled D T2 Mature tissue expressions similar to Tl expression data (data not shown). X T2 Mature tissue expression (if different expression pattern). D No expression detected X Flower Dpedicel Dreceptacle Dnectary M sepal Dpetal Dfilament Danther Dpollen Dcarpel D style D papillae Dvascular Depidermis L stomata Dtrichome X Silique D stigma D style Dcarpel D septum D placentae D transmitting tissue Dvascular Depidermis M stomata D abscission zone D ovule X in the flower and silique

T3 Seedling Expression Seedlings expressing / Seedlings screened Event-01: 1/3 Event-02: 2/3 D Scheduled X T3 Seedling tissue expressions similar to T2 seedling expression (data not shown). D T2 Mature tissue expression (if different expression pattern). GFP Expression Detected

Promoter utility Trait - Sub-trait Area: Among other uses this promoter sequence is useful to improve: Water use efficiency - Drought, heat Utility: This promoter useful for enhancing recovery after growth under water deprivation Also useful for nutrition uptake

Construct: YP0024 Promoter Candidate LD: 11768616 cDNA LD: 13614559 (Old IDs: 12324998, 5675795) Tl lines expressing (T2 seed): SR.00494-01,-03

Sequence ( SEQ ID NO : 29 ) : TGTTAAGGGAAGGTTTGCACCTAAGAATTTTGAAGGAATTTTGCGGCGATATATCAGTAA GTAACT TTCTTCTTAGTCTCAAAATTTAAGTTGCCATAAAAGTATATCAGTTTGGAGTTGTTAACC TCTTGTTT TATTATTTCTCAGCTGACTACGTCATTTGCCTTGGTTGCAAGAGCCCAGACACCATTCTC TCCAAGG AGAACCGTCTCTTCTTTCTGAGATGTGAAAAGGTATAAGTTAATCTAATTAGTCCTGATC TTGATAT GCATTCCTTTGTTTCTGTTTTACAGTTTTACTTTCTGCGCAACAAAGTAATAAAGTATTT TGTGTGTT TGAATTTGCTAATGTGATTAACGAGTGGGCTACATGGTTTTTGCAGTGTGGATCTCAACG ATCTGTG GCTCCGATCAAAACAGGGTTTGTTGCTCGTGTTAGTCGCAGGAAGACTTGAGAAATTAGA AGGTGA AGTGACCTTGGTATGGAGTTTGGAGCTATTCTACTGCTTCTGTATGAGTTTATGAGTTGA AGAAATA CTTGTCTTGTTTTTTTTATTTTGTTTTGGAATATGATTATGACTTGACTTTTAAAATGGG ATAGGATC AAAACCTTTTACTCTGTCAGGTTCATGTGGTCACCTTGAAGGTTGATTTAGTAAATCCAT GGACTTC TTTTTTGTGTTAAGATTATTCTTAGTTCAAAATTAATAGACTAATGATATTAACGTCCAC AGGCATT GCGTTCAACATCTCAAATTAAAGCGTGGAAGGCTCAGAAAGTCCAATATACACTATGTTT ATCTAC AGTTACAATCATACTACAAAAAACAAATAATGTATACGGTTTGGTCTAATATAGCCGCAT ACGATT TAGTATTTACCAACAAAAAATTGGTCTCAAACCAAACCGAACAATTGGTAATTAACAATT GTTCTT TTGGTCTTGAACCGAACCAAACCGAACTGAACTATATTAACCGACCGACTTCGTCCTTTC CTCCCCA TTGCACTGGGATCCAACAATGTCCTCCGACTCGTCCAAGATCAAGAGGAAGCGGAACCGC ATCCCn TTAAACGAAGGCG

Promoter Expression Report # 30 Promoter Tested In: Arabidopsis ihaliana, WS ecotype Spatial expression summary: Silique (H)ovule Ovule Pre-fertilization: (H)outer integument, (H)funiculus Post-fertilization: (H)outer integument, (H)funiculus Rosette Leaf (EQvascular Primary Root (H)epidermis, (H)trichoblast, (H)root hair Lateral root (H)pericycle Observed expression pattern: Strong GFP expression in upper root hairs at root transition zone and in distal vascular bundle of cotyledon. Low expression in pericycle cells of seedling root. Tl mature: No expression observed. T3 seedling: Same as T2 seedling expression. T2 mature: GFP expression in funiculus of ovules as in connective tissue between locules of anther. Expected expression pattern: Root vasculature Selection Criteria: Helariutta, et al. 2000 Cell 101: 555-567 Gene: SHR (Short-root gene) GenBank: NM 119928 Arabidopsis thaliana short-root transcription factor (SHR) (At4g37650) mRNA, complete cds gi|3069U90|ref]NM 119928.2|[306911901 Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewBin4-HAPl-GFP Marker Type: X GFP-ER Generation Screened: X Tl Mature XT2 Seedling X T2 Mature X T3 Seedling

T2 Mature Plant Expression Plants expressing / Plants screened Event-01: 1/1 Event-02: No germination D Scheduled D T2 Mature tissue expressions similar to Tl expression data (data not shown). X T2 Mature tissue expression (if different expression pattern). D No expression detected X Silique D stigma D style D carpel D septum D placentae D transmitting tissue Dvascular Depidermis Dstomata D abscission zone H ovule X Ovule Pre-fertilization: Dinner integument H outer integument D embryo sac H funiculus Dchalaza Dmicropyle Dgametophyte Post-fertilization: D zygote Dinner integument H outer integument D seed coat Dprimordia Dchalaza Dmicropyle Dearly endosperm Dmature endosperm Dembryo H funiculus X in the Ovule (Ov) of the ovary and silique X in the Funiculus (Fn) of the ovule primordia X in the pre-fertilized and fertilized ovule

T3 Seedling Expression Seedlings expressing / Seedlings screened Event-01: 2/3 Event-02: No seeds. D Scheduled X T3 Seedling tissue expressions similar to T2 seedling expression (data not shown). D T2 Mature tissue expression (if different expression pattern). GFP Expression Detected

Promoter utility Trait - Sub-trait Area: Among other uses this promoter sequence is useful to improve: Water use efficiency - Increase leaf water potential PG&D - increase root biomass, plant size Nutrient - nitrogen use efficiency, nitrogen utilization, low nitrogen tolerance Utility: This promoter is a good promoter for root nutrition uptake, root biomass.

Construct: YP0028 Promoter Candidate LD: 11768648 cDNA LD: 12561142 (0Id ID: 7093615) Tl lines expressing (T2 seed): SR00586-03.-04

Sequence (SEQ ID NO: 30):

GTCAGTGAAGTCGATTGGTAGTACTTGAAACACTTGGTTGGTTTCATGTATTTGGCC TATATATAAA CAAACATCGTAATTATATACGGATTTTTTTCGGAATTTTACGCCATATCTGTAAGTATAT ATAACAT GCATGTCGTTTTCAAATTCATATGATGAACGATCCACGTAAGTGCTACTACTCCTACAAT ATTGCAT GAGAGAGATATGTATTTATAAATTTTATTTTGAAGAAGAAATAAGAGGGAAGGTTACTTG GGTGG ATCGATGTGAAAACAAAAGAAGAAAAAGCGAAACCCACTAAGCCATTACATGATATCGAC CTTCT TATCTTTTTCCTCTTTATTTTATTTTTCTCATCTTCTTTTTGTCAGGACTTTTTTCTACT TAATGAAAC CTCCAAACTATCTAACTAATACACTCCCATGTAGAATAAAGAAAATTATATAAGATATTG TTGATA TTTTGTAACTAGAAAATATATTTGCTCTGTAATTTTTCGTAAGTTAAATCAACATTTTAA AGTAGAA ACAAATATTACTGCAAAAAGTAGGATCATTATTTTTGTCCAAAATCTCAGTTAGCTATAG GGTTGT AGTAAAAACAAAACACATTCTTGATTTGCCCCAAAAAATAAAGAGAGAGAAGAATATTGT TCAAA AGTGGTCTCTTCTCTCTCTAATTATGTTTTCACTAAACCCAATTAGATTCAAACAGTCTA CAAAGTC CAAAAGATAAACATGGGACAACAATTCGATGCAAAAAATCCTCTTTTCATGCTCTTTTTT TATTCTC TAGTCTTTTAAATTACTAATAAAAACTCACAAATCCACCAAACCCATTCTCTACAACTCA CCTTCAT CTAGATTTACCCACTCCCACCGAGAAACACAAGAAAAAAAATATACATATATAAATATAC AAGAC AACACATGATGCTGATGCAATATACACAACAAAGTATTAAATCTTAGATATTGTGGGTCT CCCTTT CTTCTATTCATTTTCTTATTCATTAAAAAAAAAAA

Promoter Expression Report # 31 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Flower (L)stomata Primary Root (H)epidermis, (H)trichoblast, (H)atrichoblast, (H)root hairs Observed expression pattern: Strong GFP expression specific to epidermal root hair trichoblast and atrichoblast cells throughout seedling root. Not expressed in lateral root. Tl mature: No expression observed. T2 mature: Low guard cell expression in flower not observed in Tl mature. T3 seedling expression: Same as T2 seedlings. Expected expression pattern: localized to the lateral root cap, root hairs, epidermis and cortex of roots. Selection Criteria: Arabidopsis public; The roles of three functional sulfate transporters involved in uptake and translocation of sulfate in Arabidopsis thaliana. Plant J. 200023:171-82 Gene: Sulfate transporter GenBank: NM 116931 Arabidopsis thaliana sulfate transporter -related (At4g08620) mRNA, complete cds gi|30680813iref|NM 116931.21[30680813I Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewBin4-HAPl-GFP Marker Type: X GFP-ER Generation Screened: XTl Mature XT2 Seedling X T2 Mature X T3 Seedling

T2 Mature Plant Expression Plants expressing / Plants screened Event-01: 1/1 Event-02: 1/1 D Scheduled D T2 Mature tissue expressions similar to Tl expression data (data not shown). X T2 Mature tissue expression (if different expression pattern). D No expression detected D Flower D pedicel D receptacle D nectary D sepal D petal D filament D anther D pollen D carpel D style D papillae D vascular D epidermis Dstomata Dtrichome X in the Guard cells of the flower

T3 Seedling Expression Seedlings expressing / Seedlings screened Event-01: 1/3 Event-02: 2/3 D Scheduled X T3 Seedling tissue expressions similar to T2 seedling expression (data not shown). D T2 Mature tissue expression (if different expression pattern). GFP Expression Detected

Promoter utility Sub-trait Area: Among other uses this promoter sequence is useful to improve: Water use efficiency - Water potential, drought, moisture stress at seed set and seed fill, water use efficiency Nutrient - nitrogen use efficiency Utility: This is good promoter forroot nutrient uptake, increase root mass and water use efficiency

Construct: YP0030 Promoter Candidate I.D: 11768642 cDNA LD: 12664333 (Old ID: 7079065) Tl lines expressing (T2 seed): SR00545-01,-02

Sequence (SEQ ID NO: 31):

AGGTCAGTGAAGTCGATTGGTACTTGCCTCATGTGTTTGGATACGAGATTACTGAACGTT GTGGTG TATTTTATAGTCATGGGTTTGTTAATTGTTATCATGCTTGCCTACTTAACTAGCGTAATT ATGTTTTT TTGTACTACCTCGGAAGTAGCTATTTTGTCGCTTATTGACAACGAGATACTTTAAGATGT TCCACAT CCACGTCGTAATCGGTTGATCGAATGGTGCCTAATAGATCAAAGTTATCCTCAACAAATA TCGATG TGTAGTATATACGTGAATATATAGTAGTCTCTTGCATGCATATCATATACAACTTAAATA CTCTTTT TGTTTCAAAATAAATAATGTTTTAGGAAAAAGATTATTGTGTCAAATTAAGTGTTGGTCT ATTCATC CAAACAAGAAAGAAAAAAAATACGAATTTGTTTTATATATCATTGACGAACAATGTTTAG CTAATA ATAAATAATTATTTATTTATAAAAATT AAAAGTTAGATAGTTTCTTAATTTAGGTGCATATAAGTTC TTTAACAAAAAAAACATTTAGGTGCATAAGTCTTAAATATCAAATATTTTGGAACAGTAA TTTTAT GTATAACTTTTTTCGTACCTATCTTCACACCGCATAAATTGCCAAAGTCAACCTTTTGAT ATTTCATT CCTCACAAAACCATATTAATTTATACACCTCAATATTGTTTAATAGTATTATCATGTTGG CTTTCGC TGAATTTATCAAAGTGCAACATGTTTTATCTTACAAAAAAATAAAAAGAAATTCACGTTG TGTGAT CTTGAGAGTTGACTTTTAAATATATCACAACTTATATAAATACGCAGCAACATTCCAATC TCTCAAG AAAATCTACAGTTCCTCCAAATAATAATACCCTCCCTCTAAGGTTTAAAACTATACCTCA TTAACAC ATTAAGAAGCTAGTCATTACTTCATTTCTATATTTTAAATAATGTTTATTGATAACAATT GCAGGCA ACTAATTTTCAGCAATCACCATTGCACTGGGATCCAACAATGTCCTCCGACTCGTCCAAG ATCAAG AGGAAGC Promoter Expression Report # 32 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Cotyledon (L)epidermis Primary Root (H)epidermis, (H)trichoblast, (H)atrichoblast Observed expression pattern: High GFP expression in epidermal cells of seedling root from hypocotyl root transition to differentiation zone. Not observed in root tip. Low GFP expression in epidermal cells of distal cotyledon. Tl mature: No expression detected. T2 mature: Guard cell expression in stem, pedicles. Low silique vascular expression. T3 seedling: Same as T2 seedlings. Expected expression pattern: predominantly expressed in the phloem Selection Criteria: Ceres microarray data Gene: putative glucosyltransferase [Arabidopsis thaliana] GenBank: BTO 10327 Arabidopsis thaliana At2g43820 mRNA, complete cds gil339420501gb]BT010327.1|[33942050] Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewBin4-HAPl-GFP Marker Type: X GFP-ER Generation Screened: X Tl Mature XT2 Seedling X T2 Mature X T3 Seedling

T2 Mature Plant Expression Plants expressing / Plants screened Event-01: 1/1 Event-02: 1/2 D Scheduled D T2 Mature tissue expressions similar to Tl expression data (data not shown). X T2 Mature tissue expression (if different expression pattern). Expression detected

T3 Seedling Expression Seedlings expressing / Seedlings screened Event-01: 2/3 Event-02: 1/3 D Scheduled X T3 Seedling tissue expressions similar to T2 seedling expression. D T2 Mature tissue expression (if different expression pattern). GFP Expression Detected X in the seedling root and root tip

Promoter utility Trait - Sub-trait Area: Among other uses this promoter sequence is useful to improve: Nutrient - nitrogen and phosphate uptake and transport Growth and Development - plant size, growth rate Utility: Promoter useful where expression in the root epidermis is important. Expression appears to be in expanded or differentiated epidermal cells.

Construct: YP0054 Promoter I.D: 13148233 (Old ID: 11768644) cDNA LD: 12348737 (Old ID: 1609253) Tl lines expressing (T2 seed): SR00549-01,-02

Sequence (SEQ ID NO: 32):

CTGATCTCTAGTCCAGTCGATTGGAGCTTATTTTGTTCTATTCTATCGTATTTGATTCTT CTTTCGTTT TTTTTTTGTTTGACTTAAGAAACCGATTGTTTATAGTAGTAAACATTTGTTTTTAATGTT GCTCGATT CCAGTGCACATGTCCAGGCTAGACACTTGTCGTTATAAAGGTTGCTTTGGTTCAATATTG ATCCACT AGAGATGTTACAACTATTGTTGACATCTGAGATTGTGTGATAAGAAAATATGAAACTGGA TTTAGT GAAAGTTACAATATATAATCATACATCATAGATAGGAAATAAGGAAATGTCAGATATACT TGAAG AATACATCAAATAGACAAGGTCCTTTTTCTTATTGTCGACTATTATAGAGCCGTACAGAA CCTTTTC ACGTCTTTAGTAATTAGTACATTCTCCATTTCGGCTCTCTCTTATTTTTTTTCCATCTCT TTTTACTTC TCCAAATAATAACAATAAAAGCTTCGATTTTGTGTGTGTTTGTATTTACATCTTGACATC GATATTC TTTTCATCAATTTTTTACCAAAAATGTAATAAAAACAAAAAAAAACCAACGCTGAACACA GACATG GTTTCTCCATCCGTTTATATTCATCGTTTGTATGTTTACTTAACAACTTATTTCAAAATA GTACATAT CATGGTTGTGTTTTTAAAAAAAGTATACAGAACAGAAAAGCACATGGTAGACAAAATAAT GAAGC CAAAATTAATACAAAGAAGAAGTTCAACTTGTATTTATTAACACATTTTCTTTCCTTGTC AAAGACA TGCAAATTGGTTTTGTTTTCTTATTCCCATTTTTTTTTTATAATAAAAAGAAGAAGAGTA AAACAAA AAAACTATCATTTCTTCTTATCGCAAAACTCTTATCTAAGCAAGAAACCGACAAAACCTA TATCTA CATATATTCTCATCAACATCTCTTGAGACATATTCATTTTGGTTAAAGCAAAAGATTTTA AGAGAG AAAGGGGGAGAAGTGAGAGAGACCATTGCACTGGGATCCAACAATGTCCTCCGACTCGTC CAAGA TCAAGAGGAAGCGGAACCGCATCCCnTTAACGAAGGCG Promoter Expression Report # 33 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Flower (M)stomata Stem (M)stomata Rosette leaf (H)stomata Observed expression pattern: GFP expression specific to stomata of primary rosette leaves. Not detected in other organs. Not detected in Tl mature. T2 mature: Expression in guard cells of flowers and stem not observed in Tl mature. T3 seedling: Same as T2 seedling expression. Expected expression pattern: Root and seed development Selection Criteria: Full-length cDNA clustering with ABI3 interacting protein2 Gene: Pseudo-response regulator 1; protein id: GenBank: NM 125531 Arabidopsis thaliana pseudo-response regulator 1, APRRl (APRR1/TOC1 family) (At5g61380) mRNA, complete cds gi]30697520|ref]NM 125531. 2|[30697520] Source Promoter Organism: Arabidopsis thaliana, WS Vector: pNewBin4-HAPl-GFP Marker Type: X GFP-ER Generation Screened: XTl Mature X T2 Seedling X T2 Mature X T3 Seedling

D Shoot apical meristem DSAM D epidermis X in the Guard cell (Gc) in the leaf

T2 Mature Plant Expression Plants expressing / Plants screened Event-01 : 0/3 Event-02: 1 /1 D Scheduled D T2 Mature tissue expressions similar to Tl expression data (data not shown). X T2 Mature tissue expression (if different expression pattern). D No expression detected X Flower Dpedicel Dreceptacle Gnectary Dsepal Dpetal Gfilament Danther Dpollen Dcarpel D style D papillae D vascular D epidermis M stomata Dtrichome X Stem D epidermis D cortex D vascular Dxylem D phloem D pith M stomata Dtrichome X in the Guard cell (Gc) of the leaf and stem

T3 Seedling Expression Seedlings expressing / Seedlings screened Event-01: 1/3 Event-02: 1/3 G Scheduled X T3 Seedling tissue expressions similar to T2 seedling expression (data not shown). D T2 Mature tissue expression (if different expression pattern). GFP Expression Detected

Promoter utility Trait Area: Among other uses this promoter sequence can be useful to improve: Water Use Efficiency - Drought, heat

Construct: YP0025 Promoter Candidate I.D: 11768641 cDNA LD: 12322657 (Old ID: 11005502) Tl lines expressing (T2 seed): SR00495-03,-04

Sequence (SEQ ID NO: 33):

gtcagtgaagtcgattgggtCTCACCATGAGCCAATGAAAAGAAATAGTAATCCAGGTGA TTGTTTCTCTTTGA TGTCTCACTTTCTAATAGCTAGTCTCTAAAAGAACCTTTGTTTTAGTGAATTCTAATATA GTAGGGG TTTTGCAGCGCAATTTTCTTCAGCACCGAAGAAAAGTAGATTGAAGATCGGAGAGTCCTC TGCTTT CTTTACATATGTCAAATCTACTGTCCTTAGAACTAACGGTCAGGATCCTCCTCTTGTCGA TGGAAAT GGCTCACTTCATCTTCATCGGGGTTTGGCGGAGAAGTTTCAAGTGGTGGCTAGTGAAGGG ATCAAC AACACCAAACAAGCACGCAGAGCAACACCAAAATCTACTGTCCTTAGAACTAACGGTCAG GATCC TCCTCTTGTCAATGGAAATGGCTCACATCATCTTCATCGGGGTGCGGCGGAAAAGTTTCA AGTGGT GGCTAGTGAAGGGATCAACAACACCAAACAAGCACACAGAAGTAGAGGGACCGAGCAATA CCATT CTCAAGGAGAGACCTTGCAGAATGGCGCCAGCTATCCACATTCCCTTGAGCGGTCACGCA CGCTTC CCACATCAATGGAATCTCATGGTAGGAACTACCAAGAGGGCAATATGAATATTCCCCAAG TTGCTA TGAACAGAAGTAAAGATTCGTCTCAAGTTGATGGATCGGGTTTCTCTGCACCAAATGCCT ATCCTT ACTATATGCATGGGGTCATGAACCAAGTTATGATGCAATCAGCAGCCATGATGCCTCAAT ATGGTC ATCAAATTCCTCATTGCCAACCAAATCATCCGAATGGAATGACGGGATATCCTTACTACC ACCACC CAATGAACACATCTTTGCAGCATAGTCAGATGTCTTTACAGAATGGTCAGATGTCTATGG TTCATC ATTCTTGGTCACCGGCAGGAAATCCGCCTTCTAATGAGGTGAGGGTAAATAAACTTGACA GAAGA GAGGAAGCTCTGCTGAAatt Promoter Expression Report # 34 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Flower (M)sepal, (M)style, (M)epidermis Stem (M)eρidermis, (H)endodermis, (H)cortex Leaf (H)mesophyll, (H)epidermis Hypocotyl (H)epidermis, (H)vascular Cotyledon (H)epidermis, (H)mesophyll Primary Root (H)epidermis, (H)trichoblast, (H)atrichoblast, (H)vascular phloem, (H)Root cap, (H)root hairs Lateral root (H)vascular, (H)cap Observed expression pattern: GFP expressed in sepals, style of silique in immature flowers, mesophyll, and epidermis of mature leaves. GFP expressed throughout epidermal layers of seedling including root tissue. Also expressed in mesophyll and epidermal tissue in distal primary leaf, and vasculature of root. Specific expression in meristematic zone of primary and lateral root. T2 Mature: Same expression as Tl mature: stem expression. T3 Seedling expression: Same as T2 seedling expression. Expected expression pattern: Shoot apical meristem Selection Criteria: Greater than 5x down in stm microarray Gene: Fructose-bisphosphate aldolase GenBank: NM 118786 Arabidopsis thaliana fructose-bisphosphate aldolase, putative (At4g26530) mRNA, complete cds gi|30687252|ref1NM 118786.2][306872521 Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewBin4-HAP 1 -GFP Marker Type: X GFP-ER Generation Screened: X Tl Mature X T2 Seedling X T2 Mature X T3 Seedling

T2 Mature Plant Expression Plants expressing / Plants screened Event-01: 1/1 Event-02: 0/5 D Scheduled X T2 Mature tissue expressions similar to Tl expression data D T2 Mature tissue expression (if different expression pattern). Expression detected X Flower Dpedicel Dreceptacle Dnectary M sepal Dpetal Dfilament Danther Dpollen Dcarpel M style D papillae D vascular M epidermis Dstomata Dtrichome X Silique P stigma D style M carpel M septum Dplacentae Gtransmitting tissue D vascular M epidermis Dstomata D abscission zone D ovule D Ovule Pre-fertilization: Dinner integument D outer integument D embryo sac D funiculus Dchalaza Dmicropyle Dgametophyte Post-fertilization: D zygote Dinner integument D outer integument D seed coat Dprimordia Dchalaza Dmicropyle Dearly endosperm D mature endosperm D embryo D Embryo Dsuspensor Dpreglobular D globular Dheart Dtorpedo Dlate Dmature Dprovascular D hypophysis D radicle D cotyledons D hypocotyl X Stem M epidermis H endodermis H cortex avascular Dxylem Dphloem Dpith Dstomata Dtrichome X in the immature flower, the sepal and the silique pedicle X in the Cortex (Cr), Endodermis (Ed) and Epidermis (Ep) in the stem

T3 Seedling Expression Seedlings expressing / Seedlings screened Event-01: 0/3 Event-02: 1/3 D Scheduled

Promoter utility Trait - Sub-trait Area: Among other uses this promoter sequence is useful to improve: PG&D - Plant size, growth rate, plant development Water use efficiency -

Construct: YP0050 Promoter Candidate I.D: 13148170 (Old ID:11768794) cDNA LD: 4909806 (Old IDs: 12340148, 1017738) Tl lines expressing (T2 seed): SR00543-01,-02

Sequence (SEQ ID NO: 34):

aatctgatctctagtccagtcgattggtaCTTGAGGGAAACATCATATTTTT AAACCTTGTCTCAGTAAGCT AACACA CACCCCTTGTGATTACTTATCCATGTTTATCCACAAGAATGCAGTTGGATTGAGATATTT TCTTCTTT GTTGAAATCAGGCCTCAAGGTGTTCATGTGGTCTGCAAAAAAATTCCCAAAAATAAAGAT AGTGAC ATCTGAAATCGATAATGGATTAGACGAAGAGTTTCGTGTTATTCCTTGGTATGGGCGGGT TTGGGG ACAGATATTTTGGCACAGACGAGGACTAGGCCACTGTGGTCCTGCAGCATTAGGTGTCCC TTCCAT GTCCTGCATTACATTTTATTGATGGATTCATCACCCTATCTACTACAACGGCTACACAAA CTATGAA GAGTTTTGTTTACTAATAAATGCCCAAGTGAGGGGTCGATCGAACCCGGGACACGTTTTT CAGTTT ACCATATAGAATTATCCTTGGAACCCTTGATACTCCATAGAACATCACCACCTCTGTTGT CATCTCA GGAATCCAGGTTCAAACCTAGTCTCTCTCTCCCTAGTGGGAGGTATATGGCCACTGGGCC AATGAT GACAAAATGCAAAAAAAATAAAATACATTTGGGTTCATTATCTAAAATATCTCTTGTGTT TGTAAG TTTTGGTTGCACACTCGTGTGGTTGAAGTGTGTGTGAGAGGTACTATACAATACACTCTG CTTTTGT TTTGTACCTATCTCTTTCTCTTCTCCACATATCCAAGACTTTGGGGATAAAGCTGAGATC ATTGGTT GCCATTTGGTTGTGTAGAAGCAATCACCCATTTGCTTTATCCGAGGTTGATAAATTTCCT CGGGTTC TCCTTCTGACACGTATGACAAATTCTAATAGTATATTCCTCGTAGATATTACCTATATAT TCTCAAT AGTTGCAGGTACTTAAGGCTTTGTCTTGGCATCCTCGTCCTCTTCAGCAAAACTCGTCTC TCTTGCA CTCCAAAAAGCAa

Promoter Expression Report # 35 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Flower (H)pedicel, (H)anther, (H)pollen, (H)vascular, (H)epidermis Stem (H)cortex, (L)vascular Hypocotyl (H)epidermis, (H)vascular, (H)phloem Cotyledon (H)vascular Primary Root (H)vascular, (H)phloem, (H)pericycle Observed expression pattern: High GFP expression throughout seedling vasculature including root. Low Expression at the base of hypocotyls. Not detected in rosette leaves. Tl mature: No expression observed. T3 seedling: Same as T2 seedling expression. T2 mature: Strong vascular and epidermal expression in floral pedicels and in developing pollen sacs of anthers. Expected expression pattern: xylem parenchyma cells of roots and leaves and in the root pericycles and leaf phloem. Selection Criteria: Arabidopsis public; The roles of three functional sulfate transporters involved in uptake and translocation of sulfate in Arabidopsis thaliana. Plant J. 2000 23:171-82 Gene: Sulfate transport GenBank: NM 121056 Arabidopsis thaliana sulfate transporter (At5gl0180) mKNA, complete cds gi|30683048|reflNM 121056.2][30683048] Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewBin4-HAPl-GFP Marker Type: X GFP-ER Generation Screened: XTl Mature X T2 Seedling X T2 Mature X T3 Seedling

T2 Mature Plant Expression Plants expressing / Plants screened Event-01 : Not screened. Event-02: 1/5 O Scheduled D T2 Mature tissue expressions similar to Tl expression data (data not shown). X T2 Mature tissue expression (if different expression pattern). Expression detected X Flower H pedicel D receptacle O nectary D sepal D petal D filament H anther H pollen D carpel D style D papillae H vascular H epidermis Dstomata Dtrichome X Stem D epidermis H cortex L vascular Dxylem D phloem D pith Dstomata Dtrichome X in the Epidermis (Ep) and Vasculature(Vs) of the inflorescence meristem X in the Pollen (Po) X in the Epidermis (Ep), Guard cell (Gc) and Cortex (Cr) of the stem

T3 Seedling Expression Seedlings expressing / Seedlings screened Event-01: Not screened. Event-02: 1/3 D Scheduled X T3 Seedling tissue expressions similar to T2 seedling expression. D T2 Mature tissue expression (if different expression pattern). GFP Expression Detected

Promoter utility Trait Area: Among other uses this promoter sequence is useful to improve: Water use efficiency - Nutrient - nitrogen use, Nutrient efficiency Plant Growth and Development - growth rate Utility: Useful for root nutrient uptake and metabolism manipulation

Construct: YP0040 Promoter Candidate I.D: 11768649 cDNA I.D: 12670159 (Old ID: 11020088) Tl lines expressing (T2 seed): SR00588-01,-02,-03

Sequence (SEQ ID NO: 35):

CCCATCACATGTAACATCATTGGGCTATCCAAAAGTCTAACCAATAATGTCAATCTATAA ACCACA TTAAGTAGTTCATTTTTTTTGTAGTCGTGTTTAGCTTGTTAAACCTCATAAAATATGTTT TCACTTAC GTTAACAAAACAAATATCTTCACGAAAAAAAATAAAATAAAATATCTTTTTGATACCGAA AAAAT AAAATAAAATAAAATAATTTTCCCTTTCGATCATAAAATGCGTAGATAAGAGAAACTGTG TTTGAG GCTCCATTTCATGTTCACCTACCAGTCTACCACGTCATTTCTCAAAGACGCAAATTTTCT AATTAGG GATGTGCTCTTTTTACATATAGATCAATATCCTAAAAAAATTTAAGATATTCATATTTTC GTACATA TATATCGAGTTTCCCGAAAAATCCATAAAAAGGGTATAATGATAGTCCTTTTTCTCCTTT AATAATA ATTTCTGAACAAAATTATATCATAATAAACTTGTGATTTTATACAAAATTTATTTGTATA TATAATT TTACTAAACAACGTGAACGATAAAAATAATATTCTCATAAAATGTTGATTAAAAATTACT TAAAAT AAATAATTATTTAGGATTATGTATTAGTAGTACTCGAACCATTTTTTTAGTTATCTGCAT GAAGACC CTAATTTTTCACATATATCGAAACTAAAACTTTGGATATACACTGTAATTTGAAAACGCT TGGAAC GGATAATGTAGTTACCTCACAAGATTTTGTACATCCCTGACATTTTATATTCATTAAAGT GTGTTTT TTTCTTCAGAAAAGAAAACACTTTTTCTTTTTGTGCTTTTAGTTTAAATTAACAAAAAAA TGGACAC CATGAGATTCCACTAACTCATGTGTATATAACATTAGGGAAGCAGTCAATTCATTTCAGC ATCCAC ACACACTTTGAATGCTCAATCAAAGCTTCTTCATAGTTAAACTTCCTCACAACGTCAAAA CTCGAG AAGAAGACCATTGCACTGGGATCCAACAATGTCCTCCGACTCGTCCAAGATCAAGAGGAA GCGGA ACCGCATCCCTTTnAACGAAGGCG Promoter Expression Report # 36 Promoter Tested In: Arάbidopsis thaliana, WS ecotype Spatial expression summary: Flower (M)stomata Silique (M)vascular, (L)stomata Stem (H)stomata Cotyledon (M)stomata Rosette Leaf (M)stomata, (M)petiole Observed expression pattern: Stomata specific expression in T2 seedling leaves. Screened under non-induced condition. Tl mature, T2 mature, T3 seedlings: Expressed in guard cells. Note: Promoter lines up to YP0060 were screened for expression in the expected target tissue only. Expression patterns in these lines may be found coincidentally. YP0060 and later promoter lines were screened for expression in all aerial tissues. This line was re-screened in newly selected Tl mature plants. Expected expression pattern: Expression found predominantly in a very small number of cells immediately adjacent to the xylem in all organs. Strong expression was observed in pollen grains. Down-regulated by light. Selection Criteria: Arabidopsis public; Arabidopsis actin-related protein 2 (AtARP2) promoter directs expression in xylem precursor cells and pollen. Plant MoI Biol. 199941:65-73 Gene: Arabidopsis actin-related protein 2 (AtARP2) GenBank: " NM 113613 Arabidopsis thaliana expressed protein (At3g26990) mRNA, complete cds gil30688587|ref|NM 113613.21[30688587I Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewBin4-HAP 1 -GFP Marker Type: X GFP-ER Generation Screened: X Tl Mature X T2 Seedling X T2 Mature X T3 Seedling

Tl Mature Plant Expression Organs/Tissues screened Events Screened: n=5 Events Expressing: n— 0 (old protocol) No GFP Expression Detected Events Screened: n=7 Events Expressing: n= 3 (new protocol) GFP Expression Detected X Flower Dpedicel Dreceptacle Dnectary Dsepal Dpetal Dfilament Danther Dpollen Dcarpel D style □ papillae D vascular D epidermis M stomata Dtrichome X Silique Dstigma Dstyle Dcarpel Dseptum Dplacentae Dtransmitting tissue M vascular D epidermis L stomata D abscission zone D ovule D Ovule Pre-fertilization: Dinner integument D outer integument D embryo sac D funiculus Dchalaza Dmicropyle Dgametophyte Post-fertilization: D zygote Dinner integument D outer integument D seed coat Dprimordia Dchalaza Dmicropyle Dearly endosperm D mature endosperm D embryo D Embryo Dsuspensor Dpreglobular Dglobular Dheart Dtorpedo Dlate Dmature Dprovascular Ohypophysis Dradicle Dcotyledons Dhypocotyl X Stem Depidermis Dcortex Dvascular Oxylem Dphloem Dpith H stomata Dtrichome D Leaf Dpetiole Dmesophyll Dvascular Depidermis Dtrichome Dprimordia D stomata D stipule D margin X in the vasculature (Vs) and Guard Cells (Gc) of the tern and flower

T2 Seedling Expression Organs/Tissues Screened Events Screened: n=2 Events Expressing: n=2 Seedlings expressing / Seedlings screened

T2 Mature Plant Expression Plants expressing / Plants screened Event-01: 1/1 Event-02: 1/2 D Scheduled D T2 Mature tissue expressions similar to Tl expression data (data not shown). X T2 Mature tissue expression (if different expression pattern). X Flower Dpedicel Dreceptacle Dnectary D sepal Dpetal D filament D anther Dpollen Dcarpel Dstyle Dpapillae Dvascular Depidermis H stomata Dtrichome Dsilique

X Silique D stigma Dstyle □ carpel D septum Dplacentae D transmitting tissue M vascular Depidermis L stomata D abscission zone D ovule X Stem Depidermis Dcortex Dvascular Dxylem Dphloem Dpith H stomata Dtrichome X in the developing flower and pedicle

T3 Seedling Expression Seedlings expressing / Seedlings screened Event-01: 1/3 Event-02: 2/3 D Scheduled X T3 Seedling tissue expressions similar to T2 seedling expression (data not shown). D T2 Mature tissue expression (if different expression pattern).

Promoter utility Trait Area: Among other uses this promoter sequence is useful to improve: Water use efficiency - Water potential, drought, moisture stress at seed set and seed fill, water use efficiency, ovule/seed abortion, Seed Yield Utility: Regulate transpiration during water stress

Construct: YP0041 Promoter Candidate I.D: 13148154 (Old ID: 11768621) cDNA LD: 12717822 (0Id ID: 4962959, 12735348) Tl lines expressing (T2 seed): SR00537-01.-02 Sequence (SEQ ID NO: 36):

gggaTGCGGTTCCGCTTCCTCTTGATCTTGGACGAGTCGGAGGACATTGTTGGATCC CAGTGCAATGG TGCAACATCGAATCCAAATTCTCTTAACATCATAACACAGACTAAAATTTCAGGCTTTCG TGTTAA AAGAGAGAATATCATTGAACTTTTTCTCTTACCTTCATGATTCCTGCAAGAACAGCACCT CCGAGGT ATACCATGTGTTTCCTTCTCGGTGGATCTTCGATCCGCAATCTGAGTTTCTGATTGATAA GAAAAAC GAAGATAAATGTTAAAAGCTGAGCTAATGACTGACAGTGATCATGGCATTATCAGAATTA TCTTTT TTACCTTCAAGCCATCTTTGTTTCCTTTTAGAACTGTGTCGAGATACCGATCCTGTATTT CTTTCTCA AGACTAGAAACAAACAGAGAATAAAAGACATTGAAACATAAAACAATGGAACTTCTTGCT GGGTT TATGATAAACAAAAAATATACGAACCGGCTAGGTAATCCTGGGTACATGGTGCTACCTCC GCTTAA AACTATGTGTTGGTAGAGCTGTGAAAAAAGAGCAAAGGGTTTAAGACAGATACATGATta gttttgtTGG ATCTGTCTTTTGAAATACCAAGCAAGCGTAAGAGTCACACGTACCATCATGcggttatca atatccatttcttgaata catcggaaaaccatgtctgccattccatcaccttcaacatcaatgagttcctgaaaaaat atttggttgtgatcagcctcgagattcttggagggttgaaactattccaaa agagaaattagcagaggacgcgatgaaggtttaccggagtaaaaagcgcttcaggtgctt ggaatctttcagtgcctactttgataaccctcccatctggaagctgat cacagttcaaaaacacaacgggttagtactctggaaaaaaaataatttggtgataatatc agctttagaggctgcaactggcacaagtatgaaactttatcgactatcg gatgaaaaatcccaa

Promoter Expression Report # 37 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Flower (L)pedicel, (L)stomata Stem (L)stomata Leaf (L)vascular, (L)stomata Cotyledon (H)mesophyll, (H)vascular, (H)epidermis Primary Root (H)root hairs Observed expression pattern: Low GFP expression in stomatal cells of stem, pedicels, and vasculature of leaves in mature plants. High GFP expression in root hairs, epidermis and mesophyll cells of seedling cotyledon. Not seen in rosette leaves. T2 mature: Same as Tl mature expression. T3 seedling: Same as T2 seedling expression. Expected expression pattern: Constitutively expressed in all green tissues Selection Criteria: Arabidopsis microarray Gene: Expressed protein [Arabidopsis thaliana] GenBank: NM 119524 Arabidopsis thaliana expressed protein (At4g33666) mRNA, complete cds gi]30689773|ref|NM 119524.2|[30689773] Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewBin4-HAPl-GFP Marker Type: X GFP-ER Generation Screened: X Tl Mature X T2 Seedling XT2 Mature X T3 Seedling

Tl Mature Plant Expression Organs/Tissues screened Events Screened: n= 3 Events Expressing: n= 2 X Flower L pedicel Dreceptacle Dnectary G sepal Dpetal D filament D anther Dpollen D carpel Dstyle Dpapillae Dvascular Depidermis L stomata Dtrichome □ Silique D stigma Dstyle D carpel D septum D placentae D transmitting tissue Dvascular Depidermis D stomata D abscission zone D ovule

T2 Mature Plant Expression Plants expressing / Plants screened Event-01: 1/1 Event-02: 0/3 D Scheduled X T2 Mature tissue expressions similar to Tl expression data . D T2 Mature tissue expression (if different expression pattern). D No expression detected X in the inflorescence meristem

T3 Seedling Expression Seedlings expressing / Seedlings screened Event-01: 1/3 Strong Event-02: 1/3 Very weak D Scheduled X T3 Seedling tissue expressions similar to T2 seedling expression. D T2 Mature tissue expression (if different expression pattern). D No GFP Expression Detected X in the Cotyledon (Co) and Root hair (Rh)

Promoter utility Trait Area: Among other uses this promoter sequence is useful to improve: PG&D Sub-trait Area: Plant size , growth rate, stay green, Utility: Useful for C/N partitioning, photosynthetic efficiency, source enhancement and seedling establishment

Construct: YP0056 Promoter Candidate LD: 11768645 cDNA LD: 12396394 (Old ID: 7083850) Tl lines expressing (T2 seed): SR00550-01

Sequence (SEQ ID NO: 37):

ATAGGAATCTGCTTCGGTAGAAGATTCGAGAGAGGAGAGGAAGCATCGGTGGTTTTG GAGTTCCTT ATTCTTCTCTTCTTTCCAAAGTTTTGTCATTCGCCAAGATTCCTTAAAAACTTGTTCACA CATCATAA TTATGCACCAATAGGTTATAAATCATAATCCAACAAGTTAGTCATTGGCTTTAATTTTAA AAAATCC CATAAGAGTAAAATCTTTTAGAAAGTTAATCAACCCACACATGGGCTAGAAAACCAAAAA CCCCA CGAACATTGAGATTACAAGAAACATTTTTAAGTCCTAAATGAGCCCAAGAGCATTGCTTA ATGAAG AAGAACTGATATTAATTAACTAATATTAGGACACATAAAAAAATACGAAAACACCAATCT TCATGC CACAAAATCAAACAAAAACGAAAAAATCAATTTTCATGAAATGGATAAAGAGAGAGCGTA ATTAT CAGGAATTTGATTGAGTACGGTTGTTATGATGATCATTCACAATTATCTTTGATCTTGAG ATTTAGC AATAGTTAATTTTCGGATGTTTTTTTGTTACTTGCTGCTCACTTCTTGTATGCAGATTAA TTTATAAG AGAGACCAGTTACAACTCTTTCTTATTTGAATAAGATTTTATAAGATGTAGTGTGGCCAT GTGGGTT TATTGCATGCAGCTCTCTGCGTTGGTCCCAAGTCCACGACAATAGAGAGTTTCTGCACTT CACGGTA TCGTCGTCGTCACAAGTTCTTTACCTTATCATTGGCACAAGTTAGCCACCGTCTTTGCGC AAGTTAG CATGTTGTGCTACATACGTGTCATGAACTGATTGGTCAAATTTGGATATATTTTATTCCC GTCGGTT ATGTTTGGATAAAAATATAAAACGGAAATTTCTGTTTCAGCCTTCCTTGGTCCCAAAGAA AAATAC GCACACCTACTCCCTTCATTCTCTATCCTCTCCACTCATAATATATACATCTAAATGCAA TCTCTCC

Promoter Expression Report # 38 --St ■■• ' Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Primary root (H)root hairs Observed expression pattern: GFP expression specific to epidermal root hairs at hypocotyl root transition zone. This line was not screened in T2 mature and T3 seedlings. Expected expression pattern: Shoot apical meristem Selection Criteria: Greater than 5x down in stm microarray Gene: hypothetical protein GenBank: NM 118575 Arabidopsis thaliana. RNA recognition motif (RRM)-containing protein (At4g24420) mRNA, complete cds gijl8416342iref]NM 118575.1|[18416342] Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewBin4-HAP 1 -GFP Marker Type: X GFP-ER Generation Screened: X Tl Mature X T2 Seedling T2 Mature T3 Seedling

Tl Mature Plant Expression Organs/Tissues screened Events Screened: n= 3 Events Expressing: n= 0 No GFP Expression Detected

T2 Mature Plant Expression Plants expressing / Plants screened Event-01: N/A Event-02: N/A □ Scheduled □ T2 Mature tissue expressions similar to Tl expression data (data not shown). □ T2 Mature tissue expression (if different expression pattern). Expression detected

T3 Seedling Expression Seedlings expressing / Seedlings screened Event-01: N/A Event-02: N/A D Scheduled D T3 Seedling tissue expressions similar to T2 seedling expression (data not shown). D T2 Mature tissue expression (if different expression pattern). GFP Expression Detected

Promoter utility Trait Area: Among other uses this promoter sequence is useful to improve: Water use efficiency; Nutrient Sub-trait Area: Plant size, growth rate, drought, water use efficiency, nitrogen utilization Utility: early establishment of Rhizobium infection by increasing expression of elicitors

Construct: YP0068 Promoter Candidate LD: 11768798 cDNA I.D: 12678173 (0Id ID: 1022896) Tl lines expressing (T2 seed): SR00598-01,-02

Sequence (SEQ ID NO : 38 ) :

AAATTGGGGAGTGGGGAGATGTTTGGTTATATTCCCTTCTCATCGATGGTCTAGATG TGCGAGGTG ACTCTCATGGAGGTAAAGAACAATGGTGATTTTGTGAAGAACCCAACGTAATGGTAATTC CTAAAA AGGTTAGAAGTTTTTTCAGCTTGTTGTATTGCTAAAATGGGGTTGATGTACTCAACGACA TCCAAGT GTACTTGAGTGAGCTTTTTTGGGGTTGAGTACCTCGACCCATTATTCAAACTAATGTAAA TGGTGA ATGCAGCAGTGACTTTGTTGCCTTTTGCAAGAACTAAAGAAGACAGAAACAGGTTGTAAA AGAGA GCCAAGTGTGTGTTTATGGTAGAAAGAGCAAAGTGAACGAAAGGTGTACCTTTTTGACTT GTTGTC ACTGGTTTTCTCCCACTTCATCCGTTTCATGCTGCATCAGAAAACAACATAAGAAATGAA TGACGT AACGCGAAGCATTAGGAGTTGCTTGTAAATTAATACATTGCCATTACTAACGTAATTCAG TAGATT CTAACTACAAATGAAGTCAATGTATCTATCTGTCTACTTTAGCCAATGTATGATAAGACC AAATAG TCTTCTCTTTTTTCAGAAACTCTCTAGGATTAAAAAGTTTGTGGGTGAAAGAAATATTAT CGTGTGG ' " ATGATAAGAATAATTGATCTTGTGTTAGTAAATTAGGAATAGATATACAAGTAGGTTTCT CTCTAA' ATAAAAAATAAAAGAGTTTAAATTGCATGCGTATAAAAGAAAAAAGTAAGAAGAAAATAT GTTCC GGTTAATGGTTGGGTGCATCCGAATCGAACCGGCGCAAACCAAAAAATCTAAAGGAGATT TGAGG TGATAAAAGGAAATCAGACATTGAACCAAAAAAACAAAAGCGAGACGGTGGAAAGAAAAA ACTG GAAAAGACAGTTTTAGCCCCTCCTAAAAGCAAAGAAAAAAAAGATAATAAATAGCTTCGT CGTCG TGATCGACCTCTGCCATTGCACTGGGATCCAACAATGTCCTCCGACTCGTCCAAGATCAA GAGGAA GCGGAACCGCATCCCGTTAAACGAAGGC

Promoter Expression Report # 39 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Primary root (H)root hairs Observed expression pattern: High GFP expression specific to epidermal root hair at hypocotyls root transition zone. Screened under non-induced condition. Tl mature: No expression detected. T2 mature: No expression detected. T3 seedling: Same expression as T2 seedlings. GFP specific to root hairs. Expected expression pattern: Heat inducible. Selection Criteria: Expression data (full chip) >30 fold induction at 42C at Ih and 6 Gene: LMW heat shock protein -mitochondrial GenBank: NM 118652 Arabidopsis thaliana mitochondrion-localized small heat shock protein (At4g25200) mRNA, complete cds gi]30686795|ref1NM 118652.2|[306867951 Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewBin4-HAP 1 -GFP Marker Type: X GFP-ER Generation Screened: X Tl Mature X T2 Seedling X T2 Mature X T3 Seedling

Tl Mature Plant Expression Organs/Tissues screened Events Screened: n= 3 Events Expressing: n=0 No GFP Expression Detected

T2 Mature Plant Expression Plants expressing / Plants screened Event-01: 0/3 Event-02: 0/3 D Scheduled D T2 Mature tissue expressions similar to Tl expression data (data not shown). D T2 Mature tissue expression (if different expression pattern). No expression detected

T3 Seedling Expression Seedlings expressing / Seedlings screened Event-01 : No data Event-02: No data D Scheduled X T3 Seedling tissue expressions similar to T2 seedling expression . D T2 Mature tissue expression (if different expression pattern). GFP Expression Detected X in the root hair

Trait Area:Among other uses this promoter sequence is useful to improve: Water use efficiency; Nutrient Sub-trait Area: Increase plant growth or seed yield under heat stress conditions, nitrogen utilization, low N tolerance Utility: Useful for root nutrient uptake

Construct: YP0082 Promoter Candidate I.D: 13148250 (Old ID: 11768604) cDNA LD: 13609100 (0Id IDs 12678209, 6462494) Tl lines expressing (T2 seed): SR00606-01,-02,-03

Sequence (SEQ ID NO: 39):

AAGGGATGCGGTTCCGCTTCCTCTTGATCTTGGACGAGTCGGAGGACATTGTTGGAT CC CAGTGCAATGGTTTGTAGAGAAACAGGAAGCTTTGTTGAGAAAGTTTTAACAGTGAGT TTAGTATTTATCTGAGAACTGTGTGCGACGAAACTGGAGACAGGGGAGGACTATATAC AGGAGTTAAGTGTTGAAGGTGGGATATTGTCAGTGAGTGAATGTTCTGGAGAAGTGGG GAGATTGATATTTCTCTGGATGTTTCTAGAGATCCGTGGCTCCCAAGCTAAGTGAAACA AATATAAATTGTCTTGTTTATTAACGGGCTACGGCCCATCGGTATTAGGCCTGTTTTAG TAGATTTATGGTCAACAAGTCAAATGACCCTAGAACATGGTACATGAATGAAAAGGAC CAAAATGTGGACAGAAATCAACCAAAGGCCGAGGCGGCCACCAACCACCGAACGTGG GAAGGTCTGGTTCGGGCAAACATTCGAAAAGACCAAGAAGACTCGGATAAACAATTAA AAAAATCAGATATTGGGGAAGGCAAATATATATTGGTGTTGAGGTCAAGACTTAGAGT TTTGACGTTATTTTAAAAAAAATATTTTTGGGTAAAGACCAAAGAGTTTGACGCTATCT TGAGGTTTATTTATTCAACGTAGACTATATACTATGGGGCCGTTCTGAATTGGACCTTTT AAGTTTTGATTTTATTAAAACGCCATGTTGTTTAGTCGAGAGATAAACAAAAGACGTGT CTCTAGCTAAGCTGACCTTCATATATGCTTTGACATAATACATTAATTATAATCTCTCTT TTTGGTGTGTGTAAAAAATACACTCTATATTTTATTATGTAGACACACTACATGCAGAA GTGTTCTTACCTCTGCCTCTACGTGCTGTCAACTCCTACAGATCTTCAAGTACCCCAACC GTTATCTAACAGATTAAATATAATATACCCCTAACTATATTATATATCACAATTGTTCA AACCAGTGTATATAGACTAGACGATTCCTTGAACCATATAATTCCAGCTTTACTATAAG AGTCTTAAGGACA

Promoter Expression Report # 40 ||f j •$' yf $', :- Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Hypocotyl (H)epidermis Primary Root (H)epidermis, (H)trichoblast, (H)root hairs Observed expression pattern: High GFP expression throughout epidermal layer of hypocotyl and upper root including root hairs. Not detected in lower root. No expression observed in Tl mature plants. T2 mature: No expression observed. T3 seedling: Same expression as T2 seedlings. Expected expression pattern: Root Selection Criteria: Genome annotation Gene: ABI3-interacting protein 2 homolog; unknown protein GenBank: NM 101286 Arabidopsis thaliana zinc finger (C3HC4-type RING finger) protein family (Atlgl4200) mRNA, complete cds gil30683647|ref|NM 101286.21[306836471 Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewBin4-HAP 1 -GFP Marker Type: X GFP-ER Generation Screened: X Tl Mature X T2 Seedling X T2 Mature X T3 Seedling

T2 Mature Plant Expression Plants expressing / Plants screened Event-01: 0/2 Event-02: 0/1 D Scheduled X T2 Mature tissue expressions similar to Tl expression data (data not shown). D T2 Mature tissue expression (if different expression pattern). Expression detected

T3 Seedling Expression Seedlings expressing / Seedlings screened Event-01 : 2/3 Event-02: Not screened D Scheduled X T3 Seedling tissue expressions similar to T2 seedling expression (data not shown). D T2 Mature tissue expression (if different expression pattern). I GFP Expression Detected

Promoter utility Trait Area: Among other uses this promoter sequence is useful to improve: PG&D Sub-trait Area: Nitrogen utilization; plant size, growth rate Utility: Useful for nutrient uptake e.g., root hairs root epidermis

Construct: YP0019 Promoter Candidate I.D: 11768613 cDNA LD: 4909291 Tl lines expressing (T2 seed): SR.00489-01,-02

Sequence (SEQ ID NO: 40):

TTACGCGGCGCTACACTCTTATCAAAGTTTGAAGATTTTTCAAGAGACACAACAGAT TCAAGA TTTTCTGGTGGCTAAACTTACAATGACAGTACATGGAGGATCTCCGCGAATGGAYTTCTG CAA TGTACTAGCGTAGAACAAACACTTTTTGTTAAAGTCATCAACCAACATAGCATAGAGTTG TTT ATCTGAACAGAACACTGAAAGTCTγGKϊTITγGTTTGTGTTCCAGTAAACTGTGTCA AAATGAA AGAAAATACTTATTAACAAGTTCGKICAAAAAAAATTCAAACITITGTGCATTATTATAT GAAA GCACTTCTAGAAAGCTACCTTCTTCCTGCγCCTCCTGTTCCTAGTTTTCGGACTCTCCA CTCGAG TGTTCCCTCTCGCTTCAATCACAAACGGCγγTACTACAGACATAGCTGATAAAAGGGT CGAAA AATCATGAACCAAGTAAGCGAAACAGAGGATAATAAACATGGAAGAAGAACAGAGTAAGA C GAATTATACCACTCACTTGTTATTCGAATTGGAAACTGGKXϊATAAGCTTTCAAACGAG TTCCG AGAATGTCAGAGACTCTAAACTGAACAGTAGAAAGAGAAGTCAAAGCAGCCATGCCAAGT AT CATTCGTAAAGCATCGAAAGTCAGAACATTACCCTCAGCGGAATTTAATCAAACACCTTC TGT GCAGGAATAATCγCTGGGGGTγTγATCAACACTCAAAAAAAACTGGAACTTTGTAAA TAAAAT TATAAATGTTCGTACCITTATGCAAAAT^^ TATGAAAGAGGATAAGGTTTTTAAATAATAAATACTAAATTGTTTTTAAAAGAAACTAA AAATGGAAAGCCTTAAGCGTCGTCAATGGTTCTAGAGTCTTCTGCAACTTTCTTTTCATG AAAC TACTGTAATCTTCTGCTAACATATATAATCγCAAACACTATCTTCTCCAATT

Promoter Expression Report # 41 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Cotyledon (M)stomata Rosette Leaf (M)petiole, (M)stomata Observed expression pattern: GFP expression specific to stomata of cotyledons and primary rosette leaves of seedlings. No expression detected in Tl mature plants. T2 mature: Same as Tl mature. No expression detected. T3 seedlings: Same expression pattern as T2 seedlings. Expected expression pattern: constitutive in all tissues Selection Criteria: cDNA cluster Gene: 4OS ribosomal protein S2 GenBank: NM 115609 Arabidopsis thaliana 4OS ribosomal protein S2 (RPS2D) (At3g57490) mRNA, complete cds gi|30694654|ref]NM 115609.2|[30694654] Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewBin4-HAPl-GFP Marker Type: X QFP-ER Generation Screened: X Tl Mature X T2 Seedling X T2 Mature X T3 Seedling

T2 Mature Plant Expression Plants expressing / Plants screened Event-01: 0/2 Event-02: 0/1 D Scheduled D T2 Mature tissue expressions similar to Tl expression data (data not shown. D T2 Mature tissue expression (if different expression pattern). No expression detected

T3 Seedling Expression Seedlings expressing / Seedlings screened Event-01: 0/1 Event-02: 1/2 D Scheduled X T3 Seedling tissue expressions similar to T2 seedling expression. D T2 Mature tissue expression (if different expression pattern). GFP Expression Detected X in the Guard cells (Gc) of the cotyledon

Promoter utility Trait Area: Among other uses this promoter sequence is useful to improve: PG&D Water use efficiency Sub-trait Area: Drought, Water used efficiency Utility: Useful for water use efficiency under drought condition

Construct: YP0084 Promoter Candidate LD: 12748730 cDNA LD: 13621692 (Old IDs- 12395524, 5668493) Tl lines expressing (T2 seed): SR00581-02,-03

Sequence (SEQ ID NO: 41):

gaTATAAGTAGAATCATTTTTTGCCGCCGTTTCTCGCT AACACACCGAAAACTGAATCAAATCTCCT AGCTCTTCTACGCAAAATCGAGTGCATCGACAATGGCGGAACGTGGTGTCGAACGTGGTG GAGAT CGCGGCGATTTCGGACGTGGATTCGGTGGTCGCGGCGGTGGAAGAGGTGGTCCGAGAGGT CGTGG TCGCCGTGCAGGTCGTGCTCCAGAGGAGGAGAAATGGGTGCCAGTGACTAAGCTTGGTCG TCTCGT AAAGGAAGGTAAGATCACAAAGATTGAGCAGATCTACCTCCATTCTCTCCCAGTCAAGGA GTACC AGATCATAGATTTACTCGTCGGTCCTTCATTGAAAGACGAAGTGATGAAAATCATGCCGG TTCAAA AACAAACCAGAGCCGGTCAGAGAACGAGATTCAAGGCCTTCATCGTCGTCGGAGATAGTA ACGGT CACGTCGGATTAGGAGTCAAATGCTCCAAGGAAGTTGCGACGGCGATCAGAGGCGCGATC ATTCT CGCGAAATTGTCTGTGGTTCCGATACGAAGAGGTTATTGGGGTAACAAGATTGGAAAACC ACATAC GGTTCCGTGTAAGGTAACCGGGAAATGTGGATCTGTTACTGTACGTATGGTTCCAGCTCC GAGAGG TTCTGGTATTGTGGCGGCTAGAGTTCCTAAGAAGGTTCTTCAATTCGCTGGAATTGATGA TGTCTTT ACTTCTTCTAGAGGATCCACCAAAACTCTTGGAAACTTCGTCAAGGTATGTACTTTCACA ATGGCTG TTTTGGTTTGATGAACTCTGAATTAGGCAGTgaaaaagTAATCATTACCAGTTAAGTGAA TTTATATTG AAGATTAGGATTTAGCTGATTGTATTGGTTTGAGCATGTGAGTTTGTGTTTAAGATTGCT TGAATTG AAATGCTTTAGGTTGTTTGATTACGCTAAATTCTGACTAATGTAATTcaaattgTTGTTg tttttttttggtc

Promoter Expression Report # 42 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Flower (L)receptacle, (L)vascular Silique (L)vascular Stem (L)vascular, (L)phloem Primary root: (H)phloem Observed expression pattern: High GFP expression specific to the seedling root phloem tissue. Tl mature: No expression was observed. T2 mature: Low expression in flower and stem vascular tissues was not observed in Tl mature. T3 seedlings: Same vascular expression exists as T2 seedlings. Expected expression pattern: Constitutive in all green tissues Selectin Criteria: cDNA cluster Gene: 40S ribosomal protein S5 GenBank: NM 129283 Arabidopsis thaliana 40S ribosomal protein S5 (RPS5A) (At2g37270) mRNA, complete cds gi|30687090|reflNM_129283.2|[30687090] Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewBin4-HAPl-GFP Marker Type: X GFP-ER Generation Screened: X Tl Mature X T2 Seedling X T2 Mature X T3 Seedling

T2 Mature Plant Expression Plants expressing / Plants screened Event-01: 1/1 Event-02: 1/1 D Scheduled D T2 Mature tissue expressions are similar to Tl expression data (data not shown). X T2 Mature tissue expression (if different expression pattern). D No expression was detected X Flower Dpedicel L receptacle Dnectary Dsepal Dpetal Dfilament Danther Dpollen Dcarpel Dstyle D papillae L vascular Depidermis Dstomata Dtrichome X Silique Dstigma Dstyle Dcarpel Dseptum Dplacentae Dtransmitting tissue L vascular Depidermis Dstomata D abscission zone D ovule X Stem D epidermis D cortex L vascular Dxylem L phloem Dpith Ostomata Dtrichome X in the Recpetacle (Re), Silique (Si) and Vasculature (Vs) of the flower X in the vascular bundle of the stem

Seedling Expression Seedlings expressing / Seedlings screened Event-01: 2/3 Event-02: no germination D Scheduled X T3 Seedling tissue expressions are similar to T2 seedling expression. D T2 Mature tissue expression (if different expression pattern). GFP Expression was Detected X in the root

Promoter utility Trait Area: Among other uses this promoter sequence is useful to improve: PG&D, Nutrient economy Sub-trait Area: Plant size, growth rate, low nitrogen tolerance, NUE Utility: Useful for root nutrient uptake, source/sink relationships, root growth

Construct: YP0087 Promoter Candidate LD: 12748731 cDNA LD: 13580795 (Old IDs: 11006078, 12581302 ) Tl lines expressing (T2 seed): SR00583-01.-02

Sequence (SEQ ID NO: 42):

TGAATTGAGTAAAATGTGTTTTCAAACAGTTAGGTGGTAGAAGGTAAAGGTAATAAC ATCATGATCTTAC TAAAAGAATTGTTGCATACTAACγATCAATATTCTCAACAACATAATATAATGTTTπT γAGGTAATT^ CATTTTAATTTTTTGTGATTAAACAATTAAACAACγCGAATGATGATGATAAAAAAAAA AAATTAACAAC TCGAATAAGTTAAAGTAGCAATACACATGTCGTTCAATTCAACCAATAAAGTAAGACTTA TATTTTTAAG AAGTTGACTAATAGCTTAATAAGTTGGAAAACITGTGTAGTTTCTTAATTCCCACGTGCA GTAAGAAATA AAAATGAAAAAAATTATTATATCCTTCCCACTCTGCGACTT^ TCAAAAATAGATAAACTCATATCACAGTTTACACATTGAAATCATAAACGATAATTATGT ATTTTGTAAT AAAAAGTTAGTTCTGAAGCTCATACITγGGATAGTCGCTAGTCGCγAATATGCTCCTT GTAATAATTAAA GTCACTACGACGCACGTCAAAGCCGATATTTAGGGCTTAATTGATGCGTGTTTTTCTTTT CATATAATAGT AATATAAATTAGTACTAATAAAGTATGATGGATGGTTGAGACAGAAAAGAAAAAAGATGA CTGTATGGT CATCATTACAAAGAAGAATGTATTCTTCATGTTCTTAAGAATAATAAAATGTCACTTGTA AATCAAGTTG GTAAGICATTTTGAGAACITγGTTCGATGCAACGTATGATGATTTATGTAGACAAAAGA TAAAACCGTATC TTCAACTATTGCCAAGAAAAGATAAAACCTAATCTAGTCAGTCTCTCAACATAAATACAA CCCAATAGCC AAACTGTGTCCAATTCGGAGAGAAACTAAACTAAAACAAAACACAAAAGCCCAACATAAG CCCAATAA AACCCATTTTATAAACAGAACATTACTAACACTCA

Promoter Expression Report # 43 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Screened under non-induced conditions Flower (KQpetal, (H)epidermis, (H)anther Stem (H)epidermis Cotyledon (EQepidermis Hypocotyl (L)eρidermis, (LQstomata Rosette Leaf (L)petiole, (L)stomata Primary Root (H)phloem, (H)vascular Observed expression pattern: Tl mature: High GFP expression in petals of developing to mature flowers and in and pollen nutritive lipid rich ameboid tapetum cells in developing anthers. T2 seedling: High GFP expression in root phloem with weak expression in epidermal tissues of seedlings. T2 mature: Same as Tl mature with additional stem epidermal expression was not observed in Tl mature plants. T3 seedling: Same as T2 seedling, however, no expression was seen in epidermal cells of hypocotyls as in T2 seedlings. Expected expression pattern: : Inducible promoter - was induced by different forms of stress (e.g., drought, heat, cold) Selection Criteria Arabidopsis microarray Gene: Putative strictosidine synthase GenBank: NM 147884 Arabidopsis thaliana strictosidine synthase family (At5g22020) mRNA, complete cds gi|30688266]ref|NM 147884.2[[30688266] Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewBin4-HAPl-GFP Marker Type: X GFP-ER Generation Screened: XTl Mature X T2 Seedling XT2 Mature X T3 Seedling

X in the Epidermis (Ep) and Guard cells (Gc) of the hypocotyl X in the Guard cells (Gc) of the petiole X in the Phloem (Ph) in the root differentiation zone

T2 Mature Plant Expression Plants expressing / Plants screened Event-01: 2/8 Event-02: 1/2 D Scheduled D T2 Mature tissue expressions similar to Tl expression data (data not shown). X T2 Mature tissue expression (if different expression pattern). D No expression detected X Flower Dpedicel Dreceptacle Dnectary Dsepal Dpetal Dfilament Danther Gpollen Dcarpel Dstyle Dpapillae Dvascular H epidermis Dstomata Otrichome Dsilique X Stem H epidermis Dcortex Dvascular Dxylem Dphloem Dpith Dstomata Dtrichome X in the Epidermis (Ep) of the pedicle and stem

T3 Seedling Expression Seedlings expressing / Seedlings screened Event-01 : 2/3 Event-02: 2/3 D Scheduled D T3 Seedling tissue expressions similar to T2 seedling expression (data not shown). X T2 Mature tissue expression (if different expression pattern). GFP Expression Detected X Cotyledon Dmesophyll Dvascular L epidermis D margin Dstomata Dhydathode X in the Epidermis (Ep) of the cotyledon

Promoter utility Trait Area: PD&G, Nutrient, seed, water use efficiency Sub-trait Area: Nutrient uptake, C/N partitioning, Source enhancement, source/sink Utility: Uuseful for nutrient uptake and transport in root, transport or mobilization of steroid reserves

Construct: YPO 180 Promoter Candidate I.D: 11768712 cDNA LD: 5787483 (Old IDs: 2918666, 12367001 ) Tl lines expressing (T2 seed): SR00902-01,-02,-03

Sequence (SEQ ID NO: 43):

CAATCTGATCTCTAGTGCCAGTCGATTGGTTATTGTTGAAACGGATGGTATCCAGATTCA TAGAGTT ATACGTTGTTGACCTCGTACAGGATGAATTCATTATCTTCTTCTTCTTTTGCAGCATGGC AGGTGAT CGATGGGTATGACTTGTGATGATAGCCATGTCCACCAAATCAGCCAAGAAAAGATCAAGA CCTCG GCTGCTTACGTTCTGTTCTATAAACGCCTTGTAGACTAAAGAAACTGAAGCGGAAAAGAC AAGAA AGAGGTATTTGCATTTTTGCCGGGTTTGGCTTATTTAAAAACATCATTGGCTTGATTCTA ATTCACT ACAAGATCAAGATGAAAGCAGCTCTGCGTTGAGGCTAATTTACAGAAGAGAGAGAGAGAG TTGGG AAGAAGAGCAAAAGACCGAGAGGACATGTTGCGGGGAATTTATTTTATTCTTACAAAAAT TGGTA TCTGATTATTTTATTAACCATATTCAATTAGAGAATAGAAGAATAGAGAAAAGCCCTTTT GTGGGA TATGGTTCTAAATTGTTGTTTAGTTCTTGTGTGTCAGTTTTGGCTCTCGTCGACCAAAGA AGATTAA AGAAACCTCTACCTTATTTTAACTCAATTCTTTTGTTTTTGCAATGTCCTTTGCTTTCCA AAATTGTT AGTCTTACTTTTCACTACTTTGATAGACATTGCCTTTGCGTTTCCCTGATTAATAAGCCA GAGTACTT AAATCAAAATTGACTGTTTTGTGCATCCTGCATCACGTTTCCAATCAGAACCATAGTGTT GTCGTTG TGTCATTATCCGAATTTAAGTGGAGACATTGGTAAGTTATTTATAAACTAATTACAATCT ATTTTTC TAATTATTTCAAATAACATATTTAAGCTCTGTAGCTTCCACTAGACGGTGAAGATTTGAA GTGAGA GCTCTCTTTGCATTGCTCACCCACCAATGGATCTACCTACCCTTCTTCTTCTTCTCCTCC TTTTAAAC CCTAAAAGTTTCCCTTTCCGTTCAACAACGCCACAATCCATTGCACTGGGATCCAACAAT GTCCTCC GACTCGTCCAAGATCAAGAGGAAGCG

Promoter Expression Report # 44 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Hypocotyl (L)epidermis Observed expression pattern: Low GFP expression in the epidermal cells of hypocotyl. Screened under non- induced conditions. No Tl mature expression was observed. T2 mature: No expression was observed. T3 seedling: Same expression as the T2 seedling seen in one of two events. Guard cell expression was observed in second event. Expected expression pattern: Induced by different forms of .stress (e.g., drought, heat, cold). Selection Criteria: Arabidopsis microarray. Induced by different forms of stress (e.g., drought, heat, cold) Gene: Berberine bridge enzyme GenBank: NM 100078 Arabidopsis thaliana FAD-linked oxidoreductase family (AtlgO198O) mRNA, complete cds gi|18378905|ref|NM 100078.1|[18378905] Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewBin4-HAPl-GFP Marker Type: X GFP-ER Generation Screened: X Tl Mature X T2 Seedling X T2 Mature X T3 Seedling

T2 Seedling Expression Tissues Screened Events Screened: n=2 Events Expressing: n=2

T2 Mature Plant Expression Plants expressing / Plants screened Event-01: 0/2 Event-02: 0/2 O Scheduled X T2 Mature tissue expressions similar to Tl expression data (data not shown). D T2 Mature tissue expression (if different expression pattern). Expression detected

T3 Seedling Expression Seedlings expressing / Seedlings screened Event-01: 1/3 Event-02: 2/3 D Scheduled X T3 Seedling tissue expressions similar to T2 seedling expression (data not shown). D T2 Mature tissue expression (if different expression pattern). GFP Expression Detected

Promoter utility Trait Area: Among other uses this promoter sequence is useful to improve: Water use efficiency; PG&D Sub-trait Area: Heat Utility: Seedling establishment under sub-optimal or marginal conditions

Construct: YP0186 Promoter Candidate LD: 11768854 cDNA I.D: 13647840 (0Id IDs: 12689527, 11437778) Tl lines expressing (T2 seed): SR00906-02,-03

Sequence (SEQ ID NO: 44):

TGGACAATTACTCTTGTGTGTATCCTγGGAGTTGCTGTTTCATATGTAAGTGGACAATT ACTCTTGTGTGT AGCCITGGAGTTTTTTTATTTACGTTATTTTGGTCAGCCTTTAATTATTTTCK^ GCCACATGCCCACATAATACATTTCGCAAATTTGATACATTATGCITTGGCCC[TGTATA TTCGGTAAAAA AAAAAGCTCACKJCTACTCTCAAAACCGGCTCTGAGTATTCGTAGGCCACAATCGAAGAA AAAAAGTGCC GATTTACATATTTTTCATACAAAAAATTAAAACTGTTATGTATTATTCAAAAGCTATTTA CATATGTTTTA CTAACACGTTπCAATATTTTCTTAATCCI^ TγCGTTGTTTTGGTTAAAGAAAAACGAAAAAAAGAGAGAGTTATTCATCCTTGCAGATA AGGCTAGGGT TGGTTGAATAAAGATGTGCATATCTTATACCACTAGACCAAAGAAACAGTCACAAGTAAA AGGCCGAAT CCTTTTTATAAAATATAAACAGACGAAAGCTAATGCTRCATGGGCTTGGCCCAAGTGCAG GICTCTCGCTA GTCGCTACCO1ACAACTATCCCATATTTAATTAGTGAAGAGTATTTTATTATTTTGGTCA ACGGGCTATCT TTGTTGACAAAACTATCCCATTGGTAAAGAAATAGCAAAATAGGCGTTTCATTCTCTATA TTTAAACTTG ATTTTATGAAGAGTTGAATAGCTGAACCAGGAAGATATTTAAGAAGCCCGTACTTCACGC TTTAACTGTC AATCGATAGATCATAATAAATGACTATCTATGGATAGGAACTATAACTGAATTCAGAAAG AATCTACTA CTACTATAAATACTAAAAGAGTATTAATACAACGGAAAAAACAAAACAAAAAAAAGGGGG AACAAGGG AGTTTCATGTTAAAAAG

Promoter Expression Report # 45 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Ovule Pre-fertilization: (H)inner integument Post-fertilization: (H)inner integument, (H)outer integument Observed expression pattern: High GFP expression specific to the inner integuments of developing pre- fertilized ovules and outer integuments at the mycropylar end of post fertilized ovules. GFP detected throughout inner integument of developing seed.at mature embryo stage. T2 seedling: No expression observed. T2 Mature: Same expression as observed in Tl mature. T3 seedling: Not screened. Expected expression pattern: Expressed in ovules and different parts of seeds Selection Criteria: Greater than 5Ox up in pi ovule microarray Gene: pectin methylesterase {Arabidopsis thaliana]. GenBank: NM_124295 Arabidopsis thaliana pectinesterase family (At5g49180) mRNA, complete cds gi|30695612|ref|NM_124295.2|[30695612] Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewBin4-HAP 1 -GFP Marker Type: X GFP-ER Generation Screened: XTl Mature X T2 Seedling X T2 Mature T3 Seedling

X in the Micropyle (Mp) and Outer integuments (Oi) of the post fertilized ovule X in the ovule containing the early embryo and mature embryo

T2 Mature Plant Expression Plants expressing / Plants screened Event-01: 2/3 Event-02: 0/3 Q Scheduled . X T2 Mature tissue expressions similar to Tl expression data (data not shown). D T2 Mature tissue expression (if different expression pattern). Expression detected

T3 Seedling Expression Seedlings expressing / Seedlings screened Event-01: Not screened. Event-02: Not screened D Scheduled D T3 Seedling tissue expressions similar to T2 seedling expression (data not shown). D T2 Mature tissue expression (if different expression pattern). GFP Expression Detected

Promoter utility Trait Area; Seed, Yield, Nutrient, cold, water use efficiency Sub-trait Area: Ovule/seed abortion, seed enhancement, seed number, seed size, total yield, seed nitrogen, cold germination and vigor Utility: Useful for improvement for seed yield, composition, moisture stress at seed set, moisture stress during seed fill

Construct: YPO 121 Promoter Candidate I.D: 11768686 cDNA I.D: 12646933 (Old IDs: 12370661, 7080188) Tl lines expressing (T2 seed): SR00805-01,-02,-03

Sequence (SEQ ID NO: 45):

TTGGATTTTTTTTTTGTTGAGTCAGCAGACCATCTAATCTCTCTTTTTCCACCACAGCCT GCTTTCTA TGAAGCATTTGGGCTTACGGTTGTGGAATCAATGACTTGTGCACTCCCAACGTTTGCTAC CTGTCAT GGTGGACCCGCAGAGATTATCGAAAACGGAGTTTCTGGGTTCCACATTGACCCATATCAT CCAGAC CAGGTTGCAGCTACCTTGGTCAGCTTCTTTGAGACCTGTAACACCAATCCAAATCATTGG GTTAAA ATCTCTGAAGGAGGGCTCAAGCGAATCTATGAAAGGTTGGCCCATTCTCCTTGACAGGCT TAACAA TACAACTTGTATCGCTTCAACAAGATGATGGCTTAATAAGGATTTTTGCATGTATAGGTA CACATG GAAGAAGTACTCAGAGAGACTGCTTACCCTGGCTGGAGTCTATGCATTCTGGAAACATGT GTCTAA GCTCGAAAGGAGAGAAACACGACGTTACCTAGAGATGTTTTACTCATTGAAATTTCGTGA TTTGGT TAGTGTAACCCACTGTTATTCTTTTGATGTCTACATCTACTTTACTTACATTATTCTTTT CTTCGGTTT GCAGGCCAATTCAATCCCGCTGGCAACAGATGAGAACTGATCATGACAGGGTAGGATTTT ATTTCC TGCACTTTCTTTAGATCTTTTGTTTGTGTTATCTTGAATAAAAATTGTTGGGTTTTGTTT CCTTCAGT GGTTTGATTTTGGACTTATTTGTGTTAATGTTGTTTTGGCTGTTCTCTTAATATCAATAA CAAATAAA TTTACTGGTTGGTATCTAAGATCTAACAATAGTTACTATTTTTAGAGGTAAAGACACCAA CCTTGTT ATATTGGTCAGAGAGCTAAAACCTTGACTTGTTGGGAAAACAAAACTCTAATGACAGAAA ATCTG ACATGATGCCTTATAATTCACAGCCTCATGTTCTACATAAATCCTAACAATAGCACTTTG TTTCT

Promoter Expression Report # 46 | , f% %f" fe ;| ' Promoter Tested In: Arahidopsis thaliana, WS ecotype Spatial expression summary: Silique (H)ovule Ovule Pre-fertilization:(H)embryo sac, (H)gametophyte Post-fertilization: (H)zygote Observed expression pattern: GFP expression is specific to female gametophyte and surrounding sporophytic tissue of pre-fertilized ovules and zygote of fertilized ovule 0-5 hours after fertilization (HAF). Not detected in developing embryos. T2 mature: Did not germinate. T3 seedlings: No seeds available. Expected expression pattern: Expressed in ovules and different parts of seeds Selection Criteria: Greater than 5Ox up in pi ovule microarray Gene: hypothetical protein GenBank: NM 123661 Arabidopsis thaliana expressed protein (At5g42955) mRNA, complete cds gill8422274|ref]NM 123661.1|[18422274] Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewBin4-HAP 1 -GFP Marker Type: X GFP-ER Generation Screened: XTl Mature X T2 Seedling T2 Mature T3 Seedling

X in the pre-fertilized ovule X in the Egg cell (Ec) and Synergid cell (Sn) of the Female gametophyte (Fgm) X in the Zygote (Zg) an Hour after fertilization (HAF)

T2 Mature Plant Expression Plants expressing / Plants screened Event-01: No germination. Event-02: No germination. D Scheduled D T2 Mature tissue expressions similar to Tl expression data (data not shown). D T2 Mature tissue expression (if different expression pattern). Expression detected

T3 Seedling Expression Seedlings expressing / Seedlings screened Event-01: No seeds available. Event-02: No seeds available. D Scheduled D T3 Seedling tissue expressions similar to T2 seedling expression (data not shown. D T2 Mature tissue expression (if different expression pattern). GFP Expression Detected

Promoter utility Trait Area: Among other uses this promoter sequence is useful to improve: Seed, yield, quality Sub-trait Area: Ovule/seed abortion, harvest index,test weight, seed size, total yield,amino acid, protein,total oil, total seed composition Utility: This is promoter is useful for enhance of seed composition, seed size, seed number and yield,etc.

Construct: YP0096 Promoter Candidate LD: 13148242 (Old ID: 11768682) cDNA LD: 4949423 (Old IDs: 12325608, 1007532) Tl lines expressing (T2 seed): SR.00775-01,-02 Sequence (SEQ ID NO: 46):

GAGGTCAGTGAGTCGATTGGTGCAAAATTGAAAAATTGAAGGGTGAAACAAATTTAA AGATAATA TCTATTAAATCCTCTAATTTTAAAAATTTAGCAAAAATTGTATTTTCTTATGGATCTGTT AGTTCAC ACGTATCTTAATTAGTACCAAATCATATCTAATGATTAGTGATAAAACTAGTTAGATATC TATATGT GTCTTTACCATTTAACTTGAATCCTTCTTCTTTTTTTTACGTAAACAACTTGAATCCTTC GTTAATAC ATAAATTTAAAGCATTTTTTCTTTAATTCTATTGATCGGTATATATTTACTATAAGTTTT AGCTCATA TGCAATTTCAAATGATATGCTTTTAAATTTTGTCTAGGTGTGATAGTTGTATCTTTAACA TAAATCT TATAGCAAAATTATACTTGATATTCTAAATTTATCTATTTGCTCTTGTGAACCTCATATT AGTCTAG AGAAACTTTGAAATCCTTTCAATTAGTTGTATGTCCAATACATTTTTACTAACATTTATT AGTCTTTT TAATTAAGATTATTGTTAGAAAAAAAAAGATTTTTTAAAAATAAATAATATGTTTTAGAT ACAATG TGAGTTAGGCTTCTTATATTTTAAAAAATAAATTTATTTCATACTTAAAAATAGTTTGGA ATTTCAA TTTATTTGGCTGAATACCATAAAATATGTCAATTTGAACCTTATACCCATTGACTATTTG GTGTTAG AAACCCTTTAACAAAAAAAAACTATTTGGTGTTAGATATCAAAATAAAAAAAGTTTAACC ATTGGT TTCTTATATTGAATTGGATATTGTTACATGTATTAAAGTTTTTTTGGTTTAATTTTGAAA CGTTGATA GAAACTATTAAGTTTAAGTTTGGTAGTATATTTATTTGTGGAAAATTTAATTGCCATTAA ATATAAC GTCAACTTTTTTTGGTTTTTTTTGAGAAGTTACGTTGTGATTTTGATTTCCTATATAAAA GTTAGATT ACGTCATTTTTTAA

Promoter Expression Report # 47 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Flower (H)pedicel, (H)stomata Silique (M)stomata Stem (M)stomata Rosette Leaf (L)stomata Primary Root (H)root hairs Observed expression pattern: Guard cell expression throughout stem, pedicels, and siliques. High GFP preferential expression to root hairs of seedlings and medium to low expression in primary rosette leaves and petioles and stems. T2 mature: Same expression as Tl mature. T3 seedlings: Same expression as T2 seedlings- Expected expression pattern: Expressed in ovules and different parts of seeds Selection Criteria: Greater than 5Ox up in pi ovule microarray Gene: hypothetical protein GenBank: NM 122878 Arabidopsis thaliana expressed protein (At5g34885) mRNA, complete cds gi|30692647|ref|NM 122878.2|[30692647] Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewBin4-HAP 1 -GFP Marker Type: X GFP-ER Generation Screened: XTl Mature X T2 Seedling X T2 Mature X T3 Seedling

Tl Mature Plant Expression Organs/Tissues screened Events Screened: n=3 Events Expressing: n= 2 GFP Expression Detected D Flower Dpedicel Dreceptacle Dnectary Gsepal Dpetal Dfilament Danther Gpollen Dcarpel Dstyle Dpapillae Dvascular Depidermis Dstomata Dtrichome D Silique Dstigma Dstyle Dcarpel Dseptum Dplacentae Dtransmitting tissue Dvascular Depidermis Dstomata D abscission zone D ovule D Ovule Pre-fertilization: Dinner integument D outer integument D embryo sac D funiculus Dchalaza Dmicropyle Dgametophyte

T2 Mature Plant Expression Plants expressing / Plants screened Event-01: Vi Event-02: 1/1 D Scheduled X T2 Mature tissue expressions similar to Tl expression data. D T2 Mature tissue expression (if different expression pattern). Expression detected X Flower H pedicel Dreceptacle Dnectary D sepal Dpetal D filament D anther Dpollen D carpel D style D papillae Dvascular Depidermis Dtrichome X Silique Dstigma Dstyle Dcaipel Dseptum Dplacentae Dtransmitting tissue Dvascular D epidermis M stomata D abscission zone D ovule X in the inflorescence meristem, pedicel and the pre-fertilized .silique

T3 Seedling Expression Seedlings expressing / Seedlings screened Event-01: 2/3 Event-02: 2/3 D Scheduled X T3 Seedling tissue expressions similar to T2 seedling expression. D T2 Mature tissue expression (if different expression pattern). GFP Expression Detected X in the Epidermis (Ep) Promoter utility Trait Area: Among other uses this promoter sequence is useful to improve: Water use efficiency, PG&D, nutrient Sub-trait Area: Drought, heat, water use efficiency, plant size, low nitrogen utilization Utility: Useful for root nutrient uptake, plant growth under drought, heat

Construct: YP0098 Promoter Candidate LD: 12758479 cDNA LD: 4906343 (Old IDs: 12662283, 1024001) Tl lines expressing (T2 seed): SR00896-01.-02

Sequence (SEQ ID NO: 47):

TATTITTATAAATCATCTTAGTAAAAGTATGT TTAATTAGTGTTAAATTAGATCγAATGATTAGTGATAAAGTTTTTAGATATCGATATAG GTGTC TTCACCATTCAACITGAATCCTTCGTCAATGTAAAATTTC ATTGGTATATAATTTTAACATATCAATCCAATG^CACTCTTAAATTATCATGTACTTTTC GATA TATGTTATGACTCACγTGTTATGAAACAATGGATTTTCACCAATTTTGGTTATTT AAGTTTTAGCTCTTGTGCAATTTTAA^ TATCTATAACATAAAACTTATAACAAAACTATACTTGATATTCAAAAATTATTGATTTGC TCTT GTGAACTTCATATTAGCCTAGAGAACTTTGAAAACCTTTCAATAAATTGTATGTCGAATA AAG TTTCACAAACATTCATCAGCCATCTCGATCAAGACTATCGTGAG^ AAATAAATAATATGTTTAAGATAAATTGTGAGTTAGGCTTCTTATATTTTAAAAATTATA TAAG TTTATACTGAAAAAGAGTTAAAATTTTCAAATTTTAAATTTATTTGGCTTAAGAACATA^ GTCAATTTGAACCTTATACCCACTAAATATTCCATGTTAGATAACGAAATAAAAGAAAAT TAA CTATCCKJTTCCTCATATCGAATCGGATATCGTCACTCGTATTC CGTCGATAAAATCATCAAACTAAAGTTTCGTAGTATATTCATCTGTCGAAAATCTATCCC CATC AAATATAACGTCAAATTCATTCGTCTTCATCAAAAAAGTCACTTCGTGATTTTGATCTCC TATAT AAAATTCAGATAACTCCAATTTCCAAATAAAAAAT

Promoter Expression Report # 48 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Flower (H)pedicel, (H)sepal, (H)vascular Silique (H)septum, (H)vascular Stem (H)vascular Leaf (H)petiole, (H)vascular, (H)phloem Hypocotyl (H)vascular Primary Root (H)vascular, (H)phloem Observed expression pattern: High GFP expression throughout mature and seedling vascular tissue. T2 mature and T3 seedling: Not screened. Expected expression pattern: Expressed in ovules and different parts of seeds Selection Criteria: Greater than 5Ox up in pi ovule microarray Gene: unknown protein; expressed protein GenBank: NM 129068 Arabidopsis thaliana expressed protein (At2g35150) mRNA, complete cds gi|30686319]reflNM 129068.2|[30686319] Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewBin4-HAPl-GFP Marker Type: X GFP-ER Generation Screened: XTl Mature XT2 Seedling T2 Mature T3 Seedling

T2 Mature Plant Expression Plants expressing / Plants screened Event-01: Not screened. Event-02: Not screened. D Scheduled D T2 Mature tissue expressions similar to Tl expression data (data not shown). D T2 Mature tissue expression (if different expression pattern). Expression detected

T3 Seedling Expression Seedlings expressing / Seedlings screened Event-01: Not screened. Event-02: Not screened. D Scheduled D T3 Seedling tissue expressions similar to T2 seedling expression (data not shown). D T2 Mature tissue expression (if different expression pattern). GFP Expression Detected

Promoter utility Trait Area: Among other uses this promoter sequence could is to improve: PG&D, nutrient, seed Sub-trait Area: Growth rate, plant size, low nitrogen use efficiency, nitrogen utilization, seed size and yield Utility: Useful for root nutrient uptake and transport, enhance plant growth rate under low nitrogen condition. Enhance plant to use water efficiently.

Construct: YPO 108 Promoter Candidate LD: 11768683 cDNA LD: 13601936 (Old IDs: 12339941 , 4768517) Tl lines expressing (T2 seed): SR00778-01,-02

Sequence (SEQ ID NO: 48):

TTAGCTGAACCAGGAAATTGATCTCTTATACCAGTTTCCGGGTTTAGATTGGTTTGA TGGCGATTTG ATTAAACCCCCGAAATTTTATGTCGTAGTTGTGCATAGTATTATTATTCTTTGCGGACAA TAGACGT ATCGGGACCAAGTTCTGTAGCAAAATTGTATAAGCTTAAGTTTGATGAAATTTAAAGGTA ATCACT AAAACCCAAATGGGACAATAAACCGGTGAAGATTTAGAGTTTTTAATTTTGACTCATGAA TCTGGA GAAAGAGCCCTCGTTAAAAGGAGTGAATCAATCCATAGGGGAAAAAGTTTTGTCTTTTTA AAAACT AAAGAACCAAACCTTAATAGAAGCAGCTCAATGTGTGACAACTTTCCACTGGCACTAAGA TAAAG TGACTAGCGATGAGTGCAATTATTGAAATAGTAGATGGTAAATATTACATACAAGAGTAA AAATA TCTTTATGTCAATGCTTAATTCAGTGTTTCTGGTTAACAAGAGAATCTTCTCTAACTTTC GTAATTG GGTCTTATAAAATTTTATGCAATTATGATTTTACCCTTTTACTACTTTTCATTAGCTTTC ACGAATCT ' ATTTTGACAAGAGAAATCATTAGAGGTAAACATGCTTTTTGGTCAAGGGCCTTAACAGTT CCACCA ATCAAGCTCAAAAGTTGTACTTAACCGACATCTTCTGTGAAAACATATAATTACATGTAC AAATCA AAACTACCTTATGAAATAAATAGAAATATTGCAGTTCATTTCTAATTTAACCTCTTCAAC TTTTAAA ACTATTTACATTTCTTTATGTCATTTCTAGTCATTTTGATGCAAATTGTACCATTTATGG ATTATCTT CACAAATTTTTAAGTTGGTGAAAACTTTTTGGTGGGTAGTTAAAACTTGAAATAGAAATT TACTTTA CCAAAATAAACTAATGAAAAGTAATCACTCCACTCCCTATAATAAGATTTCCAACGTTCC CACTAA GC

Promoter Expression Report # 49 Promoter Tested In: Arabidopsis thaliana., WS ecotype Spatial expression summary: Screened under non-induced conditions. Flower (H)septum, (H)epidermis Silique (L)carpel, (H)septum, (H)epidermis, (M)vascular Stem (M)epidermis Hypocotyl (L)epideπnis, (L)stomata Cotyledon (L)epidermis, (L)guard cell Primary Root (H) epidermis, (H)trichoblast, (H)atrichoblast, (H)root hairs Observed expression pattern: High preferential GFP expression in septum epidermal cells in siliques and root hair cells of seedlings. Low expression in cotyledon and hypocotyl epidermal cells. T2 mature: Stem epidermal and silique vascular expression observed in addition to expression observed in Tl mature. Expression in stem epidermal cells appears variable. T3 seedling: Same expression as T2 seedlings with additional guard cell expression in siliques. Expected expression pattern: Root Selection Criteria: Greater than 10x induced by Roundup. Induced in Arabidopsis microarray at 4 hours Gene: Hypothetical protein GenBank: NM_111930 Arabidopsis thaliana expressed protein (At3gl0930) mRNA, complete cds gi|306815501ref|NM_111930.2|[30681550] Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewBin4-HAPl-GFP Marker Type: X GFP-ER Generation Screened: XTl MatureX T2 Seedling X T2 Mature X T3 Seedling

T2 Seedling Expression Tissues Screened Events Screened: n=2 Events Expressing: n=2 Seedlings expressing / Seedlings screened Event-01 : No data. Event-02: No data. GFP Expression Detected X Hypocotyl L epidermis Dcortex Dvascular Dxylem Dphloem L stomata X Cotyledon Dmesophyll Dvascular L epidermis Dtrichome D margin Dstomata D Rosette Leaf Dmesophyll Dvascular Depidermis Dtrichome Dpetiole Dprimordia Dstomata D stipule D margin X Primary Root H epidermis H trichoblast H atrichoblast Dcortex Dendodermis Dvascular Dxylem Dphloem Dpericycle Dquiescent Dcolumella Dcap H root hairs D Lateral root Depidermis D initials D flanking cells Dvascular Dcap D Shoot apical meristem DSAM Depidermis X in the Epidermis (Ep) and Root Hair (Rh) of the hypocotyl X in the Epidermis (Ep) of the cotyledon I X in the Atrichoblast (At) and Trichoblast (Tr) of the root differentiation zone

T2 Mature Plant Expression Plants expressing / Plants screened Event-01: 2/2 Event-02: 2/2 D Scheduled D T2 Mature tissue expressions similar to Tl expression data (data not shown). D T2 Mature tissue expression (if different expression pattern). Expression detected X Flower Dpedicel Dreceptacle Dnectary Dsepal Dpetal Dfilament Danther Dpollen D carpel D style H septum □ papillae D vascular (H) epidermis Dstomata Dtrichome X Silique D stigma D style L carpel H septum Dplacentae D transmitting tissue M vascular H epidermis Dstomata D abscission zone D ovule X Stem M epidermis D cortex D vascular Dxylem D phloem D pith Dstomata Dtrichome X in the Epidermis (Ep) and Vasculature (Vs) of the silique X in the Epidermis (Ep) and Cortex (Cr) of the stem

T3 Seedling Expression Seedlings expressing / Seedlings screened Event-01: No germination. Event-02: 2/3 D Scheduled X T3 Seedling tissue expressions similar to T2 seedling expression. D T2 Mature tissue expression (if different expression pattern). GFP Expression Detected X in the root

Promoter utility Trait Area: Among other uses this promoter sequence is useful to improve: Water use efficiency, PG&D, nutrient, yield Sub-trait Area: Drought, growth rate, plant size, low nitrogen use efficiency, nitrogen utilization; seed yield Utility: Useful for root nutrient uptake, enhance plant growth rate under low nitrogen condition. Enhance plant to use water efficiency, useful for pod shatter

Construct: YP0134 Promoter Candidate LD: 11768684 cDNA I.D: 13489977 (Old IDs: 12332605 , 6403797) Tl lines expressing (T2 seed): SR00780-02,-03

Sequence (SEQ ID NO: 49):

CCTACTTTAGGCπTAAACAAGAAGAAAATATGACTGCTAAGTCATATTTTTCAACTCTC ATGA GCAACCGTAAAGTTGCACCGCAATATCCAACAAATGACATTCGTGTTATCTACAATCTAA TGT TGAAAATTTGKSCTCATCTAATAAAGGAGACAAAAGTTATATCTCπTCACACACACGTT AATG GAAGTGTAAAGGCGGTGAGAGTGTGGGAGAGACTTGGGGAACAAGAAGAAGGACGCGGTC A AAAAGTGACGGTGGGCTACGGCTTTTCTTGGTAGCAGTTGGAAATTCCATTAATGACTTA AAA AGTGTAAATCTTATCTTCITITTATTTTGTGATTTGATATGK^ACATTCATTTCATGA^ GTATAGTTTGATGATCATACGACAAACTTATAGGGTTCACAAAGTAGATGCAATAGTTGC ATA CCTCTGTTTAAATGTTCITGTTAATATTATAATTGATGACGGAACTCGTGAATGTTATTC AAAA TGTCCATGTAATTCAAGATCATGCACTATAATAAGTAATCTATCATTTTCAGCACAACAA TTTT GACAAAAAGTAAAAATAAAATAGAGTAAACTGATATCATATTTCCGAATTATATATATAA AC GTTTTCTGTTTCTCAATGGTCTCTTTCACTCTTGTGTTTTCTAATATTTCATTTAA^ AAACTAAGCACATCTTTGTγGATTGATTGCATTTCAACCAAAATCGATAACCGAATCAT TGTTT TTTTATGTTTTATTTCAGCTTACCACACACGTTTAGAATTTTAAAAATAAAACA^ AACTCGTTACAAATGAAAATGATATTTTTAATTCKJACTCGATGGAAAGGACCAATTTAT TCAA CACTATTGTTTAGTCCGAACACTTGCCGCGTAAGTTTTCCAACTCCCCCCATTGACCTTT CGCA CTTTCACAAACTCCGTATATATATAATGGATACACTCTCTCTTTGATCT

Promoter Expression Report # 50 Promoter Tested In: Arάbidopsis thaliana, WS ecotype Spatial expression summary: Screened under non-induced conditions Flower (H)pedicel, (L)petal, (H)silique Silique (H)carpel, (H)cortex, (H)epidermis Ovule Post-fertilization: (L)outer integument Embryo (L)mature Stem (M)epidermis, (H)cortex, (H)endodermis Leaf (H)petiole, (H)mesophyll, (H)epidermis Cotyledon (H)mesophyll, (H)epidermis Rosette Leaf (H)mesophyll, (L)vascular, (H)epidermis Primary Root (H)cortex Lateral root (H)cortex, (H)flanking cells Observed expression pattern: High preferential GFP expression in photosynthetic, cortical and epidermal tissues in mature plants and seedlings. T2 mature: Weak outer integument expression in mature ovules and mature embryo in addition to expression observed in Tl mature plants. T3 seedling: Same expression observed as T2 seedlings (seen in one event). Weak epidermal and high lateral root flanking cell expression observed in second event. Expected expression pattern: Root hairs Selection Criteria: Ceres Microarray 2.5-5X down in rhl (root hair less) mutant Gene: probable auxin-induced protein GenBank: NM 119642 Arabidopsis thaliana auxin-induced (indole-3 -acetic acid induced) protein family (At4g34760) mRNA, complete cds gi|30690121]ref1NM 119642.21[3069012Il Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewBin4-HAP 1 -GFP Marker Type: X GFP-ER Generation Screened: XTl Mature X T2 Seedling X T2 Mature X T3 Seedling

Tl Mature Plant Expression Organs/Tissues screened Events Screened: n=3 Events Expressing: n= 2 GFP Expression Detected X Flower H pedicel Dreceptacle Onectary Dsepal L petal Gfϊlament Danther Dpollen Dcarpel Gstyle H silique Dpapillae Dvascular Depidermis Dstomata Dtrichome X Silique D stigma D style H carpel H Cortex D septum D placentae D transmitting tissue

T2 Mature Plant Expression Plants expressing / Plants screened Event-01: 1/2 Event-02: 1/1 D Scheduled X T2 Mature tissue expressions similar to Tl expression data . X T2 Mature tissue expression (if different expression pattern). Expression detected X Ovule Pre-fertilization: Dinner integument D outer integument D embryo sac D funiculus Dchalaza Dmicropyle Dgametophyte Post-fertilization: D zygote Dinner integument L outer integument D seed coat Dprimordia Dchalaza Dmicropyle Dearly endosperm D mature endosperm D embryo X Embryo Dsuspensor Dpreglobular D globular D heart D torpedo Plate L mature Dprovascular Dhypophysis Dradicle Dcotyledons Dhypocotyl X in the Epidermis (Ep) and Cortex (Cr) of the stem X in the Micropyle (Mp) of the mature ovule

T3 Seedling Expression Seedlings expressing / Seedlings screened Event-01: 1/3 Same as T2 seedling. No images. Event-02: 2/6 Weak epidermis, no root expression, high lateral root flanking cell expression. D Scheduled X T3 Seedling tissue expressions similar to T2 seedling expression (data not shown). X T2 Mature tissue expression (if different expression pattern). GFP Expression Detected X Lateral root | D epidermis D initials H flanking cells D cortex D vascular D cap X in the flanking cells of the Lateral root (Lr) in the root transition zone

Promoter utility Trait Area: Among other uses this promoter sequence is useful to improve: PG&D, Nutrient; C3-C4 optimization Sub-trait Area: Low nitrogen use efficiency, nitrogen utilization, low nitrogen tolerance plant size, growth rate, water use efficiency; manipulate expression of C3-C4 enzymes in leaves Utility: Useful for root nutrient uptake and transport, enhance plant growth rate, also for enhance of plant water use efficiency Additional notes: X in the Epidermis (Ep) and Mesophyll (Me) of the leaf

Construct: YP0138 Promoter Candidate I.D: 13148247 (Old ID: 11768685 ) cDNA LD: 12333534 (Old ID: 7077536) Tl lines expressing (T2 seed): SR.00781-01,-02,-03

Sequence (SEQ ID NO: 50):

TGTGTGTCCTAAATAGTTTCTTTTTAAAATTTGTAAATACCAAGACGCGTATTTAAGAGT ATTTTGA AAAGATATTTGATTATAAAAAGAAAGAAAAAGAGAAGGCTGAGGATTAACTGCAACGTCT ACCGT TGGAAAAGAAAAACGATCAGAAAACACAGAAATTAATAAAAAGAGAGAAAAAAAAATAGA GTAT GAGAGATGCACATGGGTGCCTGCAAAAAAAAGGTAGAAGAAATTTGTCTGAAAGTGTCAC AGGCA CACTCTCTCGAACCACATTTAACAACACTCCAAACACTCTTCTTCTACTTTGTACCCTTC AGTACATT ACTCTTTCCAAAGTCCGTGATTTACGCTCTTCGATGACACCTCTCAACAGAGAGAGACTA CATGTGT ACATTTTCTTCTACCATTAAATTTTGAAGATTTTCGATGATTCAATTTAGTATATATATG GAAGATA AAATTTTCATTGTCTTTCTACATGATAGTAACGGTTTTAGAAGGGTGGTTATCACTTATA GTATTTG AGTTAAGAAATATAAAAATATACGTGACTGTTTTTCCTTGTAAACTATTTTTAGGCCCTT ATTTTTA TTCAAGTAGTCACATACGTGTTTGAAGTGTATTTAACTAAGAAAAAGAAAGTAGGAAATG AAAAG GATATAGTATTTATGGTGTAATCTTGGTAAGGACCAGGAGATCAGAAGGGGCCACAATGT CACAA AGAGGACCAACAATGAAATTAAATCCTCAGCTGGCCTTTAACATTTTGGCTCCCACCATC TCCTTCC ACACATATGCACATGTCTTCATGTCTCTCTCTCTCTATACGTTACCTACACAAATATGTA CAGACAA ATAGCCCATTACAAAATCTTTATTTATAAATATATACTCCTCAACTCCCTCAATATCCAC CCATCTC CTTCTCCATAACTCTCTCTCTCTCTCCCTAAACACAACCAAAGACTTTTATCTCTCAGGA ACCCCAA AAAC Promoter Expression Report # 51 Promoter Tested In: Arάbidopsis thaliana, WS ecotype Spatial expression summary: Screened under non-induced condition Flower (L)epidermis, (L)pedicel, (L)receptacle, (L)stomata Silique (L)stomata Stem (L)epidermis, (L)stomata Rosette Leaf (L)stomata Primary Root (H)root hairs Observed expression pattern: GFP is preferentially expressed in epidermal root hairs of hypocotyl root transition zone. Low expression in stomatal cells of stem and siliques of mature Tl plants. T2 mature: No expression detected. T3 seedling: Same as T2 seedling expression detected in one of two events. Expected expression pattern: Induced by different forms of stress (e.g., drought, heat, cold) Selection Criteria: Arabidopsis microarray Gene: putative strictosidine synthase GenBank: NM 129692 Arabidopsis thaliana late embryogenesis abundant MlO protein (At2g41280) mRNA, complete cds gi|30688595[ref|NM 129692.2|[30688595] Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewBin4-HAPl-GFP Marker Type: X GFP-ER Generation Screened: Tl Mature X T2 Seedling X T2 Mature X T3 Seedling

T2 Seedling Expression Organs/Tissues Screened Events Screened: n=2 Events Expressing: n=2 Seedlings expressing / Seedlings screened Event-01: 1/3 Event-02: 1/3 GFP Expression Detected D Hypocotyl Depidermis D cortex Dvascular Dxylem Dphloem Dstomata D Cotyledon Dmesophyll Dvascular Depidermis Dtrichome D margin Dstomata X Rosette Leaf Dmesophyll Dvascular Depidermis Dtrichome Dpetiole Dprimordia

T2 Mature Plant Expression Plants expressing / Plants screened Event-01 : 0/3 Event-02: 0/2 D Scheduled D T2 Mature tissue expressions similar to Tl expression data (data not shown). D T2 Mature tissue expression (if different expression pattern). No expression detected

T3 Seedling Expression Seedlings expressing / Seedlings screened Event-01 : 2/3 Event-02: 0/3 D Scheduled X T3 Seedling tissue expressions similar to T2 seedling expression (data not shown). D T2 Mature tissue expression (if different expression pattern). GFP Expression Detected

Promoter utility Trait Area: Stress Sub-trait Area: Utility: Among other uses this promoter sequence is useful to improve: Tolerance to stress and to increase plant growth or seed yield under stress conditions

Construct: YP0177 Promoter Candidate LD: 11768710 cDNA LD: 12735519 (Old ID: 2709268) Tl lines expressing (T2 seed): SR.00900-01,-02,-03,-04

Sequence (SEQ ID NO: 51):

tgTGTGTCCTAAATAGTTTCTTTTTAAAATTTGTAAATACCAAGACGCGTATTTAAGAGT ATTTTGAA AAGATATTTGATTATAAAAAGAAAgaaaaagagAAGGCTGAGGATTAACTGCAACGTCTA CCGTTGGA AAAGAAAAACGATCAGAAAACACAGAAATTAATAAAAAGAGAGAAAAAAAAATAGAGTAT GAGA GATGCACATGGGTGCCTGCAAAAAAAAGGTAGAAGAAATTTGTCTGAAAGTGTCACAGGC ACACT CTCTCGAACCACATTTAACAACACTCCAAACACTCTTCTTCTACTTTGTACCCTTCAGTA CATTACTC TTTCCAAAGTCCGTGATTTACGCTCTTCGATGACACCTCTCAACAGAGAGAGACTACAtg tgtACATTT TCTTCTACCATTAAATTTTGAAGATTTTCGATGATTCAATTTAGTATATATATGGAAGAT AAAATTT TCATTGTCTTTCTACATGATAGTAACGGTTTTAGAAGGGTGGTTATCACTTATAGTATTT GAGTTAA GAAATATAAAAATATACGTGACTGTTTTTCCTTGTAAACTATTTTTAGGCCCTTATTTTT ATTCAAG TAGTCACATACGTGTTTGAAGTGTATTTAACTAAGAAAAAGAAAGTAGGAAATGAAAAGG ATATA GTATTTATGGTGTAATCTTGGTAAGGACCAGGAGATCAGAAGGGGCCACAATGTCACAAA GAGGA CCAACAATGAAATTAAATCCTCAGCTGGCCTTTAACATTTTGGCTCCCACCATCTCCTTC CACACAT ATGCACATGTCTTCATGTCTCTCTCTCtctatACGTTACCTACACAAATATGTACAGACA AATAGCCCA TTACAAAATCTTTATTTATAAATATATACTCCTCAACTCCCTCAATATCCACCCATCTCC TTCTCCAT AACTCTCTCTCTCTCTCCCTAAACACAACCAAAGACTTTTATCTCTCAGGAACCCCAAAA ac

Promoter Expression Report # 52 Promoter Tested In: Arabidopsis ihaliana, WS ecotype Spatial expression summary: Flower (L)sepal, (L)vascular Rosette Leaf (L)vascular, (L)stomata Observed expression pattern: Weak GFP expression in sepal vasculature of developing flower buds. Weak expression in vasculature and guard cells of rosette leaves. Not detected in mature flowers. T2 mature: Same expression as Tl mature detected in one of two events. Vascular expression in pedicels of developing flowers. T3 seedlings: No expression detected. Expected expression pattern: Shoot apex including leaf primordia and parts of leaves Selection Criteria: Greater than 5x up in stm microarray Gene: unknown protein GenBank: NM 122151 Arabidopsis thaliana esterase/lipase/thioesterase family (At5g22460) mRNA, complete cds gi|30688485]reflNM 122151.21[30688485I Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewBin4-HAP 1 -GFP Marker Type: X GFP-ER Generation Screened: XTl Mature X T2 Seedling X T2 Mature X T3 Seedling

T2 Mature Plant Expression Plants expressing / Plants screened Event-01: 1/1 Event-02: 0/2 D Scheduled X T2 Mature tissue expressions similar to Tl expression data. D T2 Mature tissue expression (if different expression pattern). Expression detected X in the inflorescence meristem

T3 Seedling Expression Seedlings expressing / Seedlings screened Event-01 : 0/3 Event-02: 0/2 D Scheduled D T3 Seedling tissue expressions similar to T2 seedling expression (data not shown). D T2 Mature tissue expression (if different expression pattern). No GFP Expression Detected

Promoter utility Trait Area: Among other uses this promoter sequence could be useful to improve: Water use efficiency Sub-trait Area: Water use efficiency

Construct: YPO 192 Promoter Candidate ID: 11768715 cDNAI.D: 12688453 (Old IDs: 12384618, 3434328) Tl lines expressing (T2 seed): SR00908-01,-02 Utility: This is weak promoter expressed in guard cell and flower. Useful for water use efficiency

Sequence (SEQ ID NO: 52):

TCCTCCTACTGTCTGCTACGTCAACAAGTGGATTGCAATCAGACGGTGATTGTGTCTCTT TTCATTC TCTCTCTTTTACTAATTTCTCTGATAATTAAACTGAGAATGTATATTAAGAAAAAAAAAC AAAAAC AAGAGAGGAATTTTCATACACACTAACTTAAGACTCTTTGTAAGTTTTCCCAAATATGGA TTTTCTA GTATAAATATGAGTTCATTAGTTTCACCAAGCCTACAAGCATCTCTCCATCTCAAATCAT ATTCACC TAAAAATCAGGTCCCCTCTCTTTATATCTCTAACATTCTTATATCAGATCATATTTTTTG GATTTCTT GTTAAGTAACACCAATCTTTTAAAAGTGTTTTCAGGTTAATATAAAAGAATAATGATGTT TTCGGT GACGGTTGCGATCCTTGTTTGTCTTATTGGCTACATTTACCGATCATTTAAGCCTCCACC ACCGCGA ATCTGCGGCCATCCTAACGGTCCTCCGGTTACTTCTCCGAGAATCAAGCTCAGTGATGGA AGATAT CTTGCTTATAGAGAATCTGGGGTTGATAGAGACAATGCTAACTACAAGATCATTGTCGTT CATGGC CTCAACAGCTCCAAAGACACTGAATTTTCCATCCCTAAGGTTCACTCTTATTCTCAATAT TAACTCT CGTACATGTCACATGCCCATTTTCACCATTTTAGATATACAGTTTTGATACTTTACTTTG CATTTATT TTGCTATATGTAATTGAGGATATTGTTTTAATTTCTTTGGGTTTTTTTTTTTCGGCTAAA TGAGAATT CACTGTCTTTGGTTCTTGAAAAAAAAGTATTTGTTAATGGTAAACGCTAAACGCTATTTG AGTTTAT GTTTTTTCAAGAACTGAAAACGTTTTATTGAAAATATACACTTTTTTTGCTATTTATAGG AAAGCAT ATCACATCACATCTAGACGCAAACGCAAAATTGAGTTTTAAAGCAACCACAATCTTAAAT GCAATG AAA

Promoter Expression Report # 53 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Flower (H)pedicel, (H)vascular Primary Root (H)epidermis, (H)trichoblast, (H)atrichoblast, (L)root hair Observed expression pattern: Tl mature: High GFP expression specific in floral pedicel vascular tissue of developing flowers. Not detected in pedicels and stems of mature plants. High GFP expression throughout epidermal layers of primary seedling root. T2 mature: No expression in 3 plants observed . T3 seedling: Same as T2 seedling expression. Expected expression pattern: Inducible promoter - induced by different forms of stress (e.g., drought, heat, cold). Selection Criteria: Arabidopsis microarray Gene: Reticuline oxidase; berberine bridge enzyme GenBank: NM 102806 Arabidopsis thaliana FAD-linked oxidoreductase family (Atlg30700) mRNA, complete cds gi|306920211reflNM 102806.2|[30692021] Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewBin4-HAPl-GFP Marker Type: X GFP-ER Generation Screened: XTl Mature X T2 Seedling X T2 Mature X T3 Seedling

T2 Seedling Expression Organs/Tissues Screened Events Screened: n=2 Events Expressing: n=2 Seedlings expressing / Seedlings screened Event-01 : No data. Event-02: No data. GFP Expression Detected D Hypocotyl Depidermis Dcortex Dvascular Dxylem Dphloem Dstomata D Cotyledon Dmesophyll Dvascular Depidermis Dtrichome Dmargin Dstomata D Rosette Leaf Dmesophyll Dvascular Depidermis Dtrichome Dpetiole Dprimordia Dstomata D stipule Dmargin X Primary Root H epidermis H trichoblast H atrichoblast Dcortex Dendodermis Dvascular Dxylem Dphloem Dpericycle D quiescent D columella D cap L root hairs D Lateral root Depidermis D initials D flanking cells Dvascular D cap D Shoot apical meristem DSAM Depidermis X in the seedling root X in the Epidermis (Ep) and Root hair (Rh) of the hypocotyl root transition zone X in the Epidermis (Ep) and Root hair (Rh) of the root differentiation zone

T2 Mature Plant Expression Plants expressing / Plants screened Event-01 : 0/3 Event-02: 0/3 D Scheduled D T2 Mature tissue expressions similar to Tl expression data (data not shown). D T2 Mature tissue expression (if different expression pattern). No expression detected

T3 Seedling Expression Seedlings expressing / Seedlings screened Event-01: 2/3 Event-02: 2/3 D Scheduled X T3 Seedling tissue expressions similar to T2 seedling expression. D T2 Mature tissue expression (if different expression pattern). GFP Expression Detected X hi the root

Inductions: Treatment: Age: Generation: Time points: Response: ABA (lOOum) 14 d T3 8Hr No 14 d. T2 IHr, 6Hr Possible

Observed expression (Arrows point to area of potential response): ABA (lOOum): No differences between experimental and control grown seedlings. High GFP expression throughout root epidermis identical to T2, T3 seedling expression in report 53. ABA (lOOum): GFP expression restricted to hypocotyl-root transition zone in control grown seedlings. ABA treated seedlings express GFP throughout hypocotyl decreasing toward apex.

ABA (lOOum) YP0204.02.02 8Hr CONTROL Promoter utility Trait Area: PG&D, Nutrient. Seed development, yield Sub-trait Area: Plant size, growth rate, nitrogen use efficiency and utilization Utility: Very useful for root nutrient uptake, enhancement for plant growth under low nitrogen condition

Construct: YP0204 Promoter Candidate I.D: 11768721 cDNA LD: 12669615 (Old ID: 7089815) Tl lines expressing (T2 seed): YP0204 -02,-03,-04

Sequence (SEQ ID NO: 53):

AACTAATTAGGTCGTTAATTGTCCAAGGGTTTTTCATAGTTGATATAGTTCTGTTCAAAT ATAGCCA TCCTTAATCGATTCATGGGATCGTAAATTACTACTTCGAGTGTTGTAAAAAAAAATGAAA CTTCTA CATTACAAACTCGAATTTAATGCATCTGGAGTGATACTATAAAAGTAGGGATGCTCTCAG GTCGCA TTTGAGAGACACAGAAATGATTTTAATGGAATTAATATATTTTCAGTTTTTCACAAAAAA AAATTG TGTTTATAACAACTGCAGATTCAATGCTGATTTTATGAGTCTCACCTATAGAATTTATAT TTCTATA TTCATAGAGGCAGTATAGGTGTTGACCCAACATCGAAAGAACACTTCGTAAAAAATTCTT TGGAAC AAGGCTGAAAATTTACTCCCAAATTTAGCTATCCGATGAAGATAAATCATTTACCGTTTA TTAAAG AATTATCGAGATTTTAGTCAAAACCAAAAGAGATTATGAGCCTAAGATTTTGAATTTGTA TTGGTA AAGGAAATTGAACGAAAATTTCAGAAAAAAATATTAATAAATTGAACGATAGAGTTCACT TACTA CATAGTCAACTAGTGCCTAGCTATAATAGTTTCAAAAGACAAAAAAAACAAAATCGGTTA ACTACT TCCGTGACATAATTCTCATCTTGATTTTTGAATCCAGTCTAATTTGAAAAGTATATTCAA AATCTTT AAATCCATTAATGATAACTTTTATAATACGTTGACACACGCAATTGTATATACAATATTC TTGAATT TTAAATGTAAATTCTAGAATATATTGCGATCACCACACTAATCAAAATCTTTGGGACAAC TTGAAC CCACATTTGACTTTTCTTGGTCAAATATTTTGGCATCATGCATGATCTTCTCTATAAAAA CCAAAAG GCCTCAACGACATTCATAAACTCAGTCATTATATTTATTTTTGTTGTATTTCAACGTTCA ATCTCTGA AAACCATTGCACTGGGATCCAACAATGTCCTCCGACTCGTCCAAGATCAAGAGGAAGCGG AACCG CATCCCGCTC

Promoter Expression Report # 58 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Cotyledon (H)vascular, (H)epidertnis, (H)hydathode Rosette Leaf (H)hydathode Lateral root (H)initials Flower (H)carpel Silique (M)style, (M)carpel Leaf (L)vascular, (H)hydathode Observed expression pattern: T2 seedlings: High expression was seen in the epidermal and vascular tissues of cotyledons, lateral root initials and primordia. Expression was seen in the distal margin of the cotyledon and rosette leaves in the region of the hydathode which is a highly modified vascular and ground tissue that permits the release of water through a pore in the epidermis. T2 mature: High expression existed in the distal margin of cotyledons in mature embryos similar to that of T2 seedling expression. Low vascular expression can be seen in sepals and midvein leading to the hydathode region. High specific GFP expression was observed at the carpel differentiation zone early in silique development and continues through to maturity. An optical section of silique primordia shows arrows pointing to the boundary where carpel, style and placental tissues are rapidly differentiating. At maturity, expression decreases and is localized to tissues within the style. Endothelium expression was not observed as previously detected by UV-based standard fluorescent microscopy. Plants screened by confocal microscopy were 4 weeks old where inner integuments may not have differentiated into mature endothelium. Expected expression pattern: Aleurone in rice. An aleurone equivalent tissue in Arabidopsis is the endothelium of the ovule. The endothelium differentiates from the inner most layer of the inner integument of the developing ovule surrounding the embryo. Although not derived from endosperm, the endothelium is thought to provide a nutritive role for the embryo. Selection Criteria: Endosperm specificity in rice was demonstrated using GUS and GFP as reporters. Gene: GIuB-I gene; glutelin; storage protein. GenBank: X54314.1 GI:20209 Source Promoter Organism: Oiyza sativa Vector: pCRS-HT2 Marker Type: GFP-ER Generation Screened: D Tl Mature XT2 Seedling X T2 Mature D T3 Seedling

Tl Mature Plant Expression Organs/Tissues screened Events Screened: n~ Events Expressing: n= GFP Expression Detected T2 Seedling Expression Tissues Screened Events Screened: n=2 Events Expressing: n=2 Seedlings expressing / Seedlings screened Event-01: No data. Event-02: No data. D Scheduled GFP Expression Detected D Hypocotyl Depidermis Dcortex Dvascular Dxylem Dphloem Dstomata X Cotyledon Dmesophyll H vascular H epidermis Dmargin Dstomata H hydathode X Rosette Leaf □mesophyll Dvascular Depidermis Dtrichome Dpetiole Dprimordia Dstomata D stipule Dmargin H hydathode □ Primary Root Depidermis Dtrichoblast Datrichoblast Dcortex Dendodermis Dvascular Dxylem Dphloem Dpericycle D quiescent D columella D root cap D root hairs X Lateral root Depidermis Dtrichoblast Datrichoblast Dcortex Dendodermis H initials D flanking cells □ vascular D lateral root cap D Shoot apical meristem □ Shoot apical meristem

X in the Epidermis (Ep) and Vasculature (Vs) of the cotyledon X in the Epidermis (Ep) and Mesophyll (Me) of the primary leaf X in the Lateral root (Lr), lateral root initial and lateral root primordia

Trait - Sub-trait Area: Among other uses, this promoter sequence is useful to improve: Seed development-Endosperm production, seed abortion, seed composition, starch production, PGD-Root architecture, nutrient uptake and utilization

Construct: pCRS-HT2-pGluB-l Promoter Candidate LD: No data cDNA I.D: No data

Sequence (SEQ ID NO: 54):

gatctcgatttttgaggaattttagaagttgaacagagtcaatcgaacagacagttg aagagatatggattttctaagatcgggggaccgagtggaccggacgagga tgtggcctaggacgagtgcacaaggctagtggactcggtccccgcgcggtatcccgagtg gtccactgtctgcaaacacgattcacatagagcgggcagacgc gggagccgtcctaggtgcaccggaagcaaatccgtcgcctgggtggatttgagtgacacg gcccacgtgtagcctcacagctctccgtggtcagatgtgtaaaat tatcataatatgtgtttttcaaatagttaaataatatatataggcaagttatatgggtca ataagcagtaaaaaggcttatgacatggtaaaattacttacaccaatatgcctt actgtctgatatattttacatgacaacaaagttacaagtacgtcatttaaaaatacaagt tacttatcaattgtagtgtatcaagtaaatgacaacaaacctacaaatttgct attttgaaggaacacttaaaaaaatcaataggcaagttatatagtcaataaactgcaaga aggcttatgacatggaaaaattacatacaccaatatgctttattgtccgg tatattttacaagacaacaaagttataagtatgtcatttaaaaatacaagttacttatca attgtcaagtaaatgaaaacaaacctacaaatttgttattttgaaggaacacct aaattatcaaatatagcttgctacgcaaaatgacaacatgcttacaagttattatcatct taaagttagactcatcttctcaagcataagagctttatggtgcaaaaacaaa tataatgacaaggcaaagatacatacatattaagagtatggacagacatttctttaacaa actccatttgtattactccaaaagcaccagaagtttgtcatggctgagtc atgaaatgtatagttcaatcttgcaaagttgcctttccttttgtactgtgttttaacact acaagccatatattgtctgtacgtgcaacaaactatatcaccatgtatcccaag atgcttttttattgctatataaactagcttggtctgtctttgaactcacatcaattagct taagtttccataagcaagtacaaatagct

Promoter Expression Report # 60 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Ovule Post-fertilization: (L)endothelium Primary root (L)cortex Observed expression pattern: Tl mature: Expression was seen in the endothelium (the inner cell layer of the inner integument). Beginning in late embryogenesis or onset of seed maturation, the endothelium differentiates from the inner most layer of the inner integument of the developing ovule surrounding the embryo. Although not derived from endosperm, the endothelium is thought to provide a nutritive role for the embryo. T2 seedling: Low expression was seen in the root cortex near the hypocotyl root transition zone of the seedling approximately 3 days after germination. Expression was observed in 3-day old seedling due to low and delayed germination. The seedling was normally screened at 7-10 days old when all structures have developed. Expected expression pattern: Seed, flower, root Selection Criteria: Arabidopsis public Gene: Strong similarity to EREBP-2 (Ethylene-inducible DNA binding proteins that interact with an ethylene-responsive element) /product="EREBP-2 protein" GenBank: NM_ 117855 Arabidopsis thaliana ethylene responsive element binding factor 1 (At4g 17500) nxRNA, complete cds, gil306840641rei1NM_117855.2l[30684064] Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewBin4-H AP 1 -GFP Marker Type: GFP-ER Generation Screened: X Tl Mature X T2 Seedling T2 Mature T3 Seedling

T2 Mature Plant Expression Plants expressing / Plants screened Event-01: no data Event-02: no data G Scheduled 0 T2 Mature tissue expressions similar to Tl expression data (data not shown). Q T2 Mature tissue expression (if different expression pattern). Expression detected

T3 Seedling Expression Seedlings expressing / Seedlings screened Event-01: no data Event-02: no data G Scheduled D T3 Seedling tissue expressions similar to T2 seedling expression (data not shown) D T2 Mature tissue expression (if different expression pattern) GFP Expression Detected

Promoter utility Trait - Sub-trait Area: Among other uses, this promoter sequence is useful to improve: Seed development-seed abortion, seed size, seed fill

Construct: YP0008 Promoter Candidate I.D: 13148261 cDNA LD: 12679464 Tl lines expressing (T2 seed): SR00409-10,-l l

Sequence (SEQ ID NO: 55):

ctCGAGAGATGAAGTCTTAGTAATGTTTGAACAAACAATAATCACGTTTTCCATCAA ATTCGAGCAT TTAAAGTTTATATTACTACATGCCCCAAGATGATACCGTCCATCTCATCCGAAAATATTT CTGAAAT TGCGCTAAGACAACAATGTTTGCTCAAATTCGATCATTT AAAGTTTACAAATCTCTCATcaatcTTACA AACTTCTCACACTAAACAGAGGTACATATTTTCTTATAAAGACAAAAGGTTCGAACAGCT GGCTTC TCAACTCGAGTTGTTTGTCAGGGCCTCTCTTCACTAACTACAAGTTGGTACTTCAAATAT TGGTGGC TAGCTTCACGTGATATTGTCTACAAATTAAACCCATGAAAAAGCTGCATTAATTGTTCCA AGTGAA CCCTGAGGAGTGTCAATAGTCTTTGCTTTAGTGTGATCATTAAACCAAATCTCTAAATTC CTAATTT GTACTAACATTTGGAACGTATTTCCTACTCTTCTCCCTGCTCCAACTCCCAAAAATAAGA TTAGTTA GATTTCTATAACTAATATACATGTATACTCCCAAAAACAGTAAAACCATATTAATAAAGC TAATTT TGCATAGATTTATTTCGGTAAACCGGCGGTTCAAGTTGGGGAAAAAAAAGACAAACGGTC TAAAG TCATCCAAAGACAAAAAACCAAAGACAAGTTGAGAGAGACGAGACCAATCACAACATTGc ttcgtAG ATTGCGTGACATCATCCTTGACGGCTACTTTCATTTGTGTCTTATTTGGATAAAACGCAC GTGTTTA ATTCACGAACCTTCATAGCAATAAGAAATTTCCATTACTTTCATATTTTCAACTTTTTTT ATTACCcatt aCATGCTT AAAATATTAATTCACAAGTCTTTGTCAAAATTCAATATTTTCCAGGTTCATGAACCCTTT TTATCTCAATCTACTCTATAATATCTCCCTATAAATTACAAcaaaacctctttatttttc a

Promoter Expression Report # 61 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Hypocotyl (EQepidermis, (H)cortex Primary Root (L)epidermis, (M)vascular, (M)pericycle Stem (H)vascular Leaf (L)vascular, (M)epidermis, (M)stomata Observed expression pattern: Tl mature: Was not screened. T2 seedling: GFP is expressed throughout the cortical and vascular tissues of the seedling root. Highest expression was seen in the cortical and epidermal tissues at the hypocotyl root transition zone, decreasing toward the apex. T2 mature plant: Expression was seen in the leaf and stem vascular tissue. Low expression was seen in the leaf epidermal cells. Expected expression pattern: Seed, flower, root Selection Criteria: Arabidopsis public Gene: ethylene responsive element binding factor 5 (ATERF5) GenBank: NM 124094 Arabidopsis thaliana ethylene responsive element binding factor 5 (AtERF5) (At5g47230) mRNA, complete cds gi|30695137|reflNM 124094.2|[30695137] Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewBhi4-HAPl-GFP Marker Type: GFP-ER Generation Screened: DTl Mature X T2 Seedling X T2 Mature DT3 Seedling

Tl Mature Plant Expression Organs/Tissues screened Events Screened: n= Events Expressing: n= GFP Expression Detected

T2 Mature Plant Expression Events Screened: n=3 Events Expressing: n=2 D Scheduled D T2 Mature tissue expressions similar to Tl expression data (data not shown). D T2 Mature tissue expression (if different expression pattern). □ Flower D pedicel D receptacle D nectary □ sepal D petal D filament D anther D pollen D carpel D style D papillae □ vascular D epidermis D stomata D trichome D Silique D stigma D style D carpel D septum D placentae G transmitting tissue Q vascular G epidermis Q stomata Q abscission zone G ovule D Ovule Pre-fertilization: G inner integument G outer integument Q embryo sac Q funiculus G chalaza G micropyle Q gametophyte Post-fertilization: G zygote 0 inner integument Q outer integument G seed coat G primordia G chalaza Q micropyle G early endosperm G mature endosperm Q embryo D Embryo Q suspensor Q preglobular G globular G heart G torpedo Q late G mature Q provascular 0 hypophysis G radicle Q cotyledons G hypocotyl X Stem Q epidermis G cortex H vascular G xylem Q phloem Q pith 0 stomata Q trichome X Leaf G petiole Q mesophyll L vascular M epidermis G trichome G primordia M stomata Q stipule Q margin

X in the Guard cell (Gc), Epidermis (Ep) and Vasculature (Vs) of the leaf X in the Vascular bundle (Vb) of the stem

T3 Seedling Expression X Scheduled D T3 Seedling tissue expressions similar to T2 seedling expression (data not shown) D T2 Mature tissue expression (if different expression pattern).

Promoter utility ' Trait - Sub-trait Area: Among other uses, this promoter sequence is useful to improve: Nutrients-Low N tolerance, enhanced uptake, translocation, NUE improved PGD

Construct: YP0009 Promoter Candidate LD: 11768607 cDNA LD: 12719868 (Old ID 4902397) Tl lines expressing (T2 seed): SR00410-02,-03

Sequence (SEQ ID NO: 56):

aattcGTTTTTTATACTCCCTCTGTTCCAAGATACTTGATATTTTGGGTTTTTGCAC AAGAATT AAGAA AAGTAACTTTTATATTTTTAATTATTCTTTTAGTTAGTTTAATAATATTAATTTTACTTC TCTCATTTC ATTATTGGTTACAAACAAAAATAATAATGATAGTTTTTCAAAACATCAATTTTGGTGGAA CAAAtaaa aaaactcAAAATATCAAATAACTTGAAACAGAGGGAGTAGTTAATtaaaaaaagATATTT CACACTTTGACT TGGCGAAGCCTCATAACAATGAAGTTATGTATGAACTATATATGAAGTTAGAAACAATGG AAAAC AGCTTGTAAATATTCATTGTTGTATATATGTTTTTTTGGGTCAATTTGGTGCATGAACAA AAATAAA AACGTAGATGAAAACCGGATATTTTGGTGTTAACATTTGCATTTGAACTTCGTGAAAGAC GGATAA AAGCTCATTTTTGTTTTTTATTATATGGCTGCTATTAGTACACAGAGTTGAACTTTAGAA TACTAAA AATCTCGACATCTTTTATTTTATTTTTGTCAAGCATCGACATCTTTTCTGTTCAAGAAAA CGACCGC AATAGTCGAATAATATAACTCTTGGACTAGTTAATATATATTTGCGATAGATTTTCGATC TCACTTA TATCTTATAACCAAGAGACAAAAACAATATTGCAGTCAAGTACAAAACGAAAACAATCAC AATGT CGACTATAGATGAGTCGGTCATTCGATCCAACGGCTCTGAGTCCACGAAACACGCAACCA AGTGGT GCTCTCTTTTACACCAAATCATATTAtataaaacTTAAAAGAAAGAGAGGATGGTTCGTT GGCTCCTTCT TGTTCCTTAATTAATTCAAATTATATTCATCACCTCCATTGAATAAGTCCATTTCACGAC AAAGTCA CCAATGCTTCTTTTACATGTATATATacttctttccactccctcttctctactca

Promoter Expression Report # 62 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Flower (H)carpel, (L)style, (M)vascular, (M)stomata Silique (H)vascular, (L)cortex, (L)style, (H)septum Leaf (H)vascular, (H)hydathode Primary Root (H)vascular (H)phloem Observed expression pattern: Tl mature: Target tissues were screened. No expression was observed. T2 seedling: High expression existed in the vasculature of the seedling root. T2 mature: Strong GFP expression was seen in the siliques and vasculature of mature leaves. Vasculature expression was seen throughout the leaf including the hydathode secretory gland which discharges water from the interior leaf. Hydathodes are thought to release water actively as true glands and passively under control of root pressure. Expression of GFP is seen in guard cells and vasculature of prefertilized siliques and cortical cells of style and septum in mature siliques. Expression appears as a gradient, strongest hi septum and weakening toward style. Expected expression pattern: Seed, flower, root Selection Criteria: Arabidopsis public Gene: Contains similarity to ethylene responsive element binding factor GenBank: NM 125553 Arabidopsis thaliana AP2 domain transcription factor, putative (At5g61600) mRNA, complete cds gi|306975571ref]NM...12S553.2][30697557] Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewBin4-HAPl-GFP Marker Type: GFP-ER Generation Screened: Tl Mature XT2 Seedling XT2 Mature T3 Seedling

Tl Mature Plant Expression Organs/Tissues screened Events Screened: Events Expressing: GFP Expression Detected

X in the Septum (Sp) of the silique X in the Hydathode (Hd) of the leaf X in the Mesophyll (Me) of the adaxial leaf X in the Vasculature (Vs) and Hydathode (Hd) of the abaxial leaf

T3 Seedling Expression Seedlings expressing / Seedlings screened Event-01: no data Event-02: no data D Scheduled D T3 Seedling tissue expressions similar to T2 seedling expression (data not shown). D T2 Mature tissue expression (if different expression pattern). GFP Expression Detected

Promoter utility Trait - Sub-trait Area: Among other uses, this promoter sequence can be useful to improve: Nutrients- Modify N transport, sugar transport PGD

Construct: YPOOlO Promoter Candidate LD: 11768608 cDNA LD: 12370148 (Old ID: 4907378) Tl lines expressing (T2 seed): SR00411-02,-03

Sequence (SEQ ID NO: 57):

aattcGTCTTGCATTGGTAATCCAATATTCCATGTGATGCTTTTCTTTCTAACTGAA GGTATTCGTGAA AACGAATTCAAGATTGTGGGAGATAATTAGGGTTTCGTCAAAAGCATGAATTAGGAAAAT TtgggatT GATTTTTTGAATTTGGGAAAAGTCCTAATTTTAATTCGATCAAAGCTTAAATGATGTCGT TTTGGGC TGACTAACGAAGTCGTTAACAGGTGCCAACGAGTGTTAAAAATCTGTTATCGCTTGATAA ctctttgGT TTTTTAGTCTAATCAACACATTCTCATGTTTCAAACAGTTAATCAACATATTGTTCATGT TAAAAAA TTAACACATGTGAAATTGATAtataaaaatATCATATATTTTCAAAAGTTGGATCATTAA AtAAAAATTTT CCCTATTTTTGAAATAATTCTAAAACAGTATTAAAGATATTTCCTGAAATTGTTTGCATG TGATCGG TTTTGGACCGAAACTAAAAAACTTAGAAACTATATTAACTTTGAGTTGTCGAGATGAAGA TGTGAA ATAGAAATATAGGATCAATGGTTAGAATTTTGGCAAATGTATGAAGGTGTGTTTGATTGA ATATTC CAAGTCCTTTGCCTTTTGGAATAGGCATAACTACTACAACAAAGTTTTGATAGGTTTTCC GAATTCT TTAAACTCCTTAAATTTTTAATACATCTCAATCAAACTCCACTTATACTAAAAATAATCC ATATTGC ATTTTTAAAAatcctAAAAGAAGAATCACATGATAACTTGATAAGCACTTTT AAATGATAGATGTCCA CGTAGAGGAATAAGACAAAAAGACAAAATAAAGAAAAAGGACGAAATCTAAAGAGAGAAA ATAA GTAACAAGTCCAAGAAAAGGTAGTATGATCTTTCTCGGTCCGATCCTCGAAACTCCCTCG AAGGCT CGAACCCTCTCTTTGTTTTTTTaccactatataagaaagtccgattcctcgtcactct

Promoter Expression Report # 64 Promoter Tested In: Arabidopsis ilialiana, WS ecotype Spatial expression summary: Flower (H)pollen Silique (H)ovule Ovule Pre-fertilization: (H)gametophyte Post-fertilization: (H)embryo Embryo (H)preglobular, (H)globular, (H)hearta (H)torpedo, (M)late, (Immature, (L)cotyledons Primary Root (L)cortex Lateral root (H)primordia Observed expression pattern: Tl mature: Strong GFP expression was seen in the embryo sac early in ovule development through female gametophyte development. Expression seen before fertilization in the central cell, egg apparatus and in antipodal cells which degenerate before fertilization. GFP emissions rapidly degenerate within the egg sac post fertilization. High GFP expression is detected in cotyledons of heart stage embryos (approximately 60 hours after fertilization) and continues through embryo growth phase. No expression existed in mature embryos. High GFP is also seen in mature pollen. T2 seedling: High GFP expression was detected in mature root and lateral root primordia. Weak expression was detected in cortical cells at the root tip. Expected expression pattern: Roots Selection Criteria: Ceres Microarray. 1Ox up in Arabidopsis root tips (enzymatic digestion), 2 of 2 reps 2.5 - 5x up in Yiwen's corn root tips (cut), 2 of 2 reps Gene: 60s 17a RIBOSOMAL Protein GenBank: NM 130329 Arabidopsis thaliana 60S ribosomal protein L7A (RPL7aA) (At2g47610) mRNA, complete cds gi|30690728|refjNM 130329.21[30690728] Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewBin4-HAP 1 -GFP Marker Type: GFP-ER Generation Screened: X Tl Mature X T2 Seedling D T2 Mature DT3 Seedling

T2 Seedling Expression Tissues Screened Events Screened: n=2 Events Expressing: n=2 Seedlings expressing / Seedlings screened

T2 Mature Plant Expression Plants expressing / Plants screened Event-01: no data Event-02: no data □ Scheduled □ T2 Mature tissue expressions similar to Tl expression data (data not shown). □ T2 Mature tissue expression (if different expression pattern). expression detected

T3 Seedling Expression Seedlings expressing / Seedlings screened Event-01: no data Event-02: no data D Scheduled □ T3 Seedling tissue expressions similar to T2 seedling expression (data not shown). D T2 Mature tissue expression (if different expression pattern). GFP Expression Detected Promoter utility Trait - Sub-trait Area: Among other uses, this promoter sequence can be useful to improve: Seed development-seed abortion, seed fill, seed size, seed composition Nutrients-nutrient uptake PGD-root number, root size

Construct: YP0039 Promoter Candidate LD: 11768622 cDNA LD: 13603142 Tl lines expressing (T2 seed): SR00536-02,-03

Sequence (SEQ ID NO: 58):

ccgttcgagtatttgaaaatttcgggtacacccgcctaaataggcggaccttatctagta tatatatacatttgaactatattgtttactTTTTagttgATTTAGG CTATgtcatGACATtgacATAAATCTACCTGTTATTTATCACGtgtaatTCGtgtaaagt gtaaactagaaagttcaaatacgtattt gtttttgttctgttatataggattgtcatagttgtaaatctacaatttattacaacatga ataagtacacaagcaatgtaattggatttaattgctaaactctttacatggtcaat ctaaatttgataagaaatacgtcacatattactaagactgatagtttttttg#gtcacca attatttttgttaaattgacgaaaacaattccaaaaactcaaatgtacaaaatc atacagtctcacaaacatctcatagagaaagatataaatctcccatatgggaacgataac acgaggtcgaaatactattcgtaaaactaaaacgccttagttataaatc gttagttgtaaccgcggtcgagaatacatacagatccacgaaactactactacacatgct gctgaattggaatttggaaaagaccatcttctttaggaagagctcacc caatgagtgacaaaggtgtcggtggcttgttttctacccatatgtatacatcaaatggta gtttcattaacgtttggttttgagaaaagtaagacTTTGGCTAGT AGCTAGGTTCGTATATAATAAACTCTTTTGAGAAAGTTCATCACTGGTGGAAAATGTTAA ACCGGT TTTTTCTCATTTTTTCCGCCATGTTAACCACCGGTTTAAAAAGACCGTAACACATTGAAA GATTAAT AAGGGTATATTTGTAATTACGGTTTGCTGGCAATTTTTAATTATTATTTT AATTAGAGAAAATAGAG AAGCCCTATCAATGTACATGGTATATATATAAAAGGCAAAACCCTAGAAAACgatactat tcgactcagccgtcc tt

Promoter Expression Report # 66 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Silique (H)placentae, (L)ovule Ovule (L)chalaza Hypocotyl (L)epidermis Primary Root (L)root hairs Observed expression pattern: Tl mature: High specific expression was seen in the funiculus of placenta and the ovule attachment site to funiculus. T2 seedling: Low epidermal expression was seen in the hypocotyl near pedicles of cotyledons and root hairs. Expected expression pattern: Drought inducible Selection Criteria: Expression data, >10 fold induction under drought conditions Gene: Unknown protein GenBank: NM 101993 Arabidopsis thaliana F-box protein family (Atlg21410) mRNA, complete cds gi|30687347[ref]NM 101993.2|[30687347] Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewBin4-HAPl-GFP Marker Type: GFP-ER Histone-YFP Generation Screened: X Tl Mature X T2 Seedling D T2 Mature D T3 Seedling

X in the Funiculus (Fn) X in the Chalaza (Ch) of the mature ovule

T2 Mature Plant Expression X Scheduled D T2 Mature tissue expressions similar to Tl expression data (data not shown). D T2 Mature tissue expression (if different expression pattern).

T3 Seedling Expression X Scheduled D T3 Seedling tissue expressions similar to T2 seedling expression (data not shown). D T2 Mature tissue expression (if different expression pattern).

Promoter utility Trait - Sub-trait Area: Among other uses, this promoter sequence could be useful to improve: Seed development-seed abortion, seed size, seed fill Nutrients-nutrient uptake

Construct: YP0070 Promoter Candidate I.D: 13148246 cDNA LD: 13612791 Tl lines expressing (T2 seed): SR00600-02,-10

Sequence (SEQ ID NO: 59):

ttggcgTTAATCGTTAAGAAAAAAACGTGCCTACCTAGCCAAATGACATCTTCCTCTTTT GTGTTTAGC ACAAGTGAATGATCCAAATATCATCTACCTAATATGACCCATAAAATAGTATGTGATTGT CTTTGG ATGTTATCCCATAACATAACTTAAATTTGGGATGCATGCATATATTATATACTTCATATA AAATGAG TGGACCTCATAAGTTGCGGTTTCGATTTTTATCAAAAACAACAGTTGTCCACTTTTTGAT TTTGACA CacacccaCATTCAAGCATCTCCTTTCTTCTATATTATTACTCAAAGAAT AACACCCCCTAAGGATAAC ACCATTACACAAATCAATTCTCAGTTAATCATTTGTTTCAATATATGTTAGTACTTAAGA AATATTC GAATAGTTTTACGTATTCAAATTTTATAAATTCAGTGAATGTTTAATTACCAAGTCATTT TGGCTTG GAATCTATTCAATTATTCAAAAAAATAAATAAATAATACTGCATTTTAACGTATCAGCCA GTCAAT ATATTACACGTGTCATAAAGCATTACCCACACGTTTCTCCTGCCTCTTTCCCTTTCATTT TTTTTTTTC ATTTTTTTTGTTTCTTCTCTTGTTGTTGTTATCTTCTTCTAGCTAGCTCTGTGAAACACT TCAAATGA ACAAATATATCAAATAATAATAGTGTAATTAAGTCGGAGGAAAACAACAAGAACCCAGAA AGAGG AACGAAGAAACTAATTCAAAGGTATGATcttttattcTTTGAGAGAATTATGTTTTCTTT GTAGCAAATCC TCTGGTCTTACGTTTAGTCACTGAATTTGTTCCACTTCTGGAAAAATCTAGACACcaaaa gCAATCTAT GAACTTTAATTTCCAATCACTACCGTCTTTTGAGGTTGTAAAATCGGTAAATGGCAGATT TTTCACT CTCATTCCTTTGTGGTGATAGGATCAAGTTCaccgcttcattttagtcattaaacaaaga

Promoter Expression Report # 68 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Flower (H)pedicel, (H)receptacle, (H)nectary, (H)sepal, (H)filament, (L)anther, (H) pollen, (H)carpel, (H)style, (H)epidermis Silique (H)style, (H)carpel, (H)septum, (H)placentae, (H)epidermis, (H)abscission zone, (H)ovule Ovule Pre-fertilization: (H)inner integument, (H)outer integument, (H)funiculus, (H)chalaza, (H)micropyle Post-fertilization: (L)inner integument, (L)outer integument, (H)embryo Embryo (H)suspensor, (H)heart, (H)torpedo, (H)mature, (H)hypophysis, (H)radicle, (H)cotyledons Stem (H)epidermis, (H)cortex, (H)vascular, (H)pith Leaf (H)mesophyll, (H)vascular, (H)epideπnis Hypocotyl (H)epidermis, (H)cortex, (H)vascular Cotyledon (H)vascular, (H)epidermis Rosette Leaf (H)mesophyll, (H)vascular, (H)epidermis Primary Root (H)epidermis, (H)cortex, (H)endodermis, (H)vascular, (H)pericycle, (H)cap, (H)root hairs Lateral root (H)epidermis, (H)cortex, (H)endodermis, (H)initials, (H)vascular, (H)cap Observed expression pattern: Tl mature: Expressed strongly throughout the inflorescence meristem, shoot apical meristem and all of the floral organs. High expression was seen throughout all tissues of the pre-fertilized silique including the ovary and ovule primordial. Not expressed in the endosperm. Expression decreases in the outer and inner integuments of fertilized ovule at heart stage embryo. High expression first observed in heart stage embryo, suspensor and mycroplyar apparatus. High expression in embryo continues through to mature embryo with preferential expression at the root meristem. High expression was detected through all tissues of leaf and stem. T2 seedling: High expression was detected in the epidermal, vascular, and photosynthetic tissues of seedling. No expression was detected in the cortex cells of the hypocotyl. Expected expression pattern: Constitutive Selection Criteria: Arabidopsis public Gene: 4QS ribosomal protein S5 GenBank: NM 180233 Arabidopsis thaliana 4OS ribosomal protein S5 (RPS5B) (At3gll940) mRNA, complete cds gi|306819671ref1NM 180233.1|r306819671 Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewBin4-HAPl-GFP Marker Type: GFP-ER Generation Screened: X Tl Mature X T2 Seedling T2 Mature T3 Seedling

T2 Mature Plant Expression Plants expressing / Plants screened Event-01: no data Event-02: no data D Scheduled DT2 Mature tissue expressions similar to Tl expression data (data not shown). DT2 Mature tissue expression (if different expression pattern). Expression detected

T3 Seedling Expression Seedlings expressing / Seedlings screened Event-01: no data Event-02: no data D Scheduled DT3 Seedling tissue expressions similar to T2 seedling expression (data not shown). DT2 Mature tissue expression (if different expression pattern). GFP Expression Detected

Promoter utility Trait - Sub-trait Area: Among other uses, this promoter sequence can be useful to improve: PGD-size, growth rate, source strength, nutrient economy, yield, stress tolerance

Construct: YP0086 Promoter Candidate LD: 13148255 cDNALD: 12420894 Tl lines expressing (T2 seed): SR00582-10,-l l

Sequence (SEQ ID NO: 60):

cttatcctttaacaatgaacaggtttttagaggtagcttgatgattcctgcacatgtgat cttggcttcaggcttaattttccaggtaaagcattatgagatactcttatatctc uacatacttttgagataatgcacaagaacttcataactatatgctttagtttctgcattt gacactgccaaattcattaatctctaatatctttgttgttgatctttggtagaoat gggtactagaaaaagcaaactacaccaaggtaaaatacttttgtacaaacataaactcgt tatcacggaacatcaatggagtgtatatctaacggagtgtagaaacat ttgattattgcaggaagctatctcaggatattatcggtttatatggaatctcttctacgc agagtatctgttattccccttcctctagctttcaatttcatggtgaggatatgca gftttctttgtatatcattcttcttcttcrttgtagcttggagtcaaaatcggtte^ gtaccaattgaaacaccagctttttgagttctatgatcactgacttggttctaaccaaaa aaaaaaaaatgtttaATTTACAT ATCT AAAAGT AGGT TTAGGGAAACCTAAACAGTAAAATATTTGTATATTATTCGAATTTCACTCATCATAAAAA CTTAAA TTGCACCATAAAATTTTGTTTTACTATTAATGATGTAATTTGTGTAACTTAAGATAAAAA TAATATT CCGTAAGTTAACCGGCTAAAACCACGTATAAACCAGGGAACCTGTTAAACCGGTTCTTTA CTGGAT AAAGAAATGAAAGCCCATGTAGACAGCTCCATTAGAGCCCAAACCCTAAATTTCTCATCT ATATAA AAGGAGTGACATTAGGGTTTTTGTTCGTCCTCTT AAAGcttctcgttttctctgccgtctctc

Promoter Expression Report # 69 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Ovule Post-fertilization: (H)embryo Embryo (H)suspensor, (H)heart, (H)torpedo, (H)late, (H)mature, (H)hypophysis, (H)radicle, (EQcotyledons Rosette Leaf (H)mesophyll, (H)epidermis Primary Root (L)epidermis, (H)cap Lateral root (H)epidermis, (H)initials, (H)cap Observed expression pattern: Tl mature: High expression was seen throughout heart stage through mature embryo with preferential expression in the embryonic root meristem. T2 seedling: High expression was detected in epidermal and mesophyll cells of the rosette leaf and not detected in the cotyledon. Low expression was seen throughout root epidermal cells with high preferential expression in the lateral and primary root tip. Expected expression pattern: Constitutive Selection Criteria: Arabidopsis public Gene: ribosomal protein S2 GeiiBank: NM_104618 Arabidopsis thaliana 4OS ribosomal protein S2 (RPS2A) (Atlg58380) mRNA, complete cds gil30696258|ref]NM 1046182\ [30696258] Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewBin4-H AP 1 -GFP Marker Type: GFP-ER Generation Screened: X Tl Mature X T2 Seedling D T2 Mature D T3 Seedling

T2 Mature Plant Expression Plants expressing / Plants screened Event-01: no data Event-02: no data D Scheduled D T2 Mature tissue expressions similar to Tl expression data (data not shown). D T2 Mature tissue expression (if different expression pattern). Expression detected

T3 Seedling Expression Seedlings expressing / Seedlings screened Event-01: no data Event-02: no data D Scheduled D T3 Seedling tissue expressions similar to T2 seedling expression (data not shown). D T2 Mature tissue expression (if different expression pattern). GFP Expression Detected

Promoter utility Trait - Sub-trait Area: Among other uses, this promoter sequence can be useful to improve: Seed development-seed abortion, seed size, seed fill, seed composition Nutrients-root size, root number Construct: YP0088 Promoter Candidate LD: 13148240 cDNA LD: 13619634 Tl lines expressing (T2 seed): SR00584-10

Sequence (SEQ ID NO: 61):

tcgattgggattactacttcatctagtaaggttctgaaaacgtttgttgttgataag gaagattcgtctcaggttattactgttgatcttcaaggtttgtgattgtgacgcttat acatgtgctgaaactgtggtgtttatttattgaaaacaaaaaaaaagtctctcttgtagt ttcattgtactaaatagaaaacaagaaacgtttttttctttaatcttctacattga taatattggatcaaaggattgtttctgcaagacacaacacaaacatacttatactagttt acttctactaagtactaactacatacccatacacacacttgcacctagacttt acttctagacatcattaccctaaggtagaaccaagcttacaagcaagttttaccgacaac tcttacattacaactctagtctgtagtctttaacgtagacttactaactagt cattagtggtttaattttttaaattttcatccatatgtttttgttgtagatataaactaa agtcgGTCACATTT AATAATTGTCATTATGTCCGCG TAAAAGTCAATTCAGCTATTGGACATTTATGAAATGTAAGATTTTCTCTCTCATTTCCCC GTGCGTG AAGACATGCATTGGTTTTTCTGTAATAATCAACAAATCCAAACCCCTTTTCGATCTTTAT TTGGACatt gttagagacaaaatttctctatagTCTTTTTCCTAATTTGATACCATgtttttgtttcTG CACAAatttacTCACtggtttaactaacTAT CCACTTatttatGATTTTACCattaggcgtcaGCTAGCCctagtCAAatttgtAAACAAG CCAAGCTATCTACAtaaatcga gatgtcATT AACGTT AATCGTCgttaatTCGAATTTgaaaacaTAGATAGctttagcagtacaatgggcaatggt aagaagaatagca aaagGCCCAAtatttgGTTTGCAgaaattaAAGCCTtaaaaaaaagcCCACAGATATTTG TCAAAgaaccctaAT

Promoter Expression Report # 70 #tr Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Silique (L)ovule Ovule Pre-fertilization: (L)gametophyte Post-fertilization: (L)embryo sac Leaf (L)mesophyll Hypocotyl (H)epideimis, (H)cortex Primary Root (M)epidermis, (H)cortex, (H)caρ, (L)root hairs Lateral root (H) epidermis Observed expression pattern: Tl mature: Low expression was observed in the region of the central cell of the developing female gametophyte of prefertilized ovules. GFP levels appear to be degrading entering maturity and are not detected in the mature gametophyte. T2 seedlings: High epidermal expression was detected in the root hypocotyl transition zone decreasing toward the root elongation zone, then increasing again at the root cap. Expected expression pattern: Expressed in ovules and different parts of seeds Selection Criteria: Greater than 5Ox up in pi ovule microarray Gene: putative calmodulin GenBank: NM 106315 Arabidopsis thaliana calcium-binding EF- hand family protein (Atlg76650) mRNA, complete cds gi|30699187|ref|NM 106315.2|[30699187] Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewBin4-HAP 1 -GFP Marker Type: GFP-ER Generation Screened: XTl Mature X T2 Seedling T2 Mature T3 Seedling

Tl Mature Plant Expression Organs/Tissues screened Events Screened: n= 8 Events Expressing: n= 2 GFP Expression Detected D Flower D pedicel D receptacle D nectary D sepal D petal D filament D anther D pollen D carpel D style D papillae D vascular D epidermis D stomata D trichome X Silique D stigma D style D carpel D septum D placentae D transmitting tissue D vascular

T2 Mature Plant Expression Plants expressing / Plants screened Event-01: no data Event-02: no data G Scheduled Q T2 Mature tissue expressions similar to Tl expression data (data not shown). Q T2 Mature tissue expression (if different expression pattern). Expression detected

T3 Seedling Expression Seedlings expressing / Seedlings screened Event-01: no data Event-02: no data D Scheduled D T3 Seedling tissue expressions similar to T2 seedling expression (data not shown). D T2 Mature tissue expression (if different expression pattern). GFP Expression Detected

Promoter utility Trait - Sub-trait Area: Among other uses, this promoter sequence can be useful to improve: Seed development- endosperm production, seed abortion, seed size

Construct: YPOlOl Promoter Candidate I.D: 11768659 cDNA I.D: 13616623 (Old ID: 1378049) Tl lines expressing (T2 seed): SR00708-01,-10

Sequence (SEQ ID NO: 62):

Ttctcgttctctagaatattgctggaccggattaggtcaatattattgggccagatt agatattgaattgtcgacgttgcttacgttacgttatatcttgtttaagaattaaac ctatcgacttagtcttaattaagaaaacattgccttaaattctctggtctgcgaccgtti itttgaccgttaacccctaattaaagaaacaaaataattatagaaagagcact gaaatgtgattartttaacagtactcrMgagaaaattcgtactttttagtttttttttt^ attttcgttggctcataatctttctttctcggtccgggactaaccgttggcaaaaaaaaa aaaaaagttgacaataattattaaagcgtaaatcatacctctcaaataaaaa cttgaatttggaaacaaagacaactaaaaaactcgaatttaagagaattcctaaaatcaa gtgaagtatcatcacttggtaaaatttcataaccgttggcttctatttctat gtgtgccttggtttgcaggagataatatttcatttccaaccaatgatattcgtacacata gtcaaacaaatgtttgtctttgttattatattgagaaagaaacaagaaagag agagagagatagataagacgaaggaagtgaagcttccaagcgcccaccgttaaaaatctc gtgtgcaagtttcaaatacaagtggccggtggtctccataatttgat cgtcatccaattaaaaaggaagaaaaagcgtgttttaTACAAGAAAACTCATTAAAAtag caagtctagaaatatctcaacACT AATCTa ccacgTCTATTACACACACACACACACacacttgATCTTAATTTATTTTCAAGATTCAAG AAAATACCCAT TCCattaccaCAACTtgaccacacgCCT AT AT Ataaaacataaaagccctttcccc

Promoter Expression Report # 71 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Silique (H)ovule Ovule Pre-fertilization: (L)primordia, (L)inner integument, (L)outer integument, (L)funiculus Pre-fertilization: (H)outer integument, (H)seed coat Hypocotyl (M) epidermis Cotyledon (L)vascular Primary Root (H)root hairs Observed expression pattern: Tl mature: High expression was detected in the outer integuments of ovules. Expression can be detected in low levels in primordia to mature stage ovules and seed. T2 seedlings: Medium expression was detected in the epidermal cells of the hypocotyl and root. Root epidermal expression does not extend to the root cap. Low expression was seen in the vasculature of cotyledons. Expected expression pattern: Integument Selection Criteria: Gene: "amine acid permease" (At5g09220) GenBank: NM 120958 Arabidopsis thaliana amino acid permease 2 (AAP2) (At5g09220) mRNA, complete cds gi|30682579|ref]NM_120958.2|[30682579] Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewBin4-HAPl-GFP Marker Type: GFP-ER Generation Screened: X Tl Mature X T2 Seedling D T2 Mature D T3 Seedling

T2 Mature Plant Expression Plants expressing / Plants screened Event-01: no data Event-02: no data 1 Scheduled □ T2 Mature tissue expressions similar to Tl expression data (data not shown). D T2 Mature tissue expression (if different expression pattern). Expression detected

T3 Seedling Expression Seedlings expressing / Seedlings screened Event-01: no data Event-02: no data D Scheduled D T3 Seedling tissue expressions similar to T2 seedling expression (data not shown). D T2 Mature tissue expression (if different expression pattern). GFP Expression Detected

Promoter utility Trait - Sub-trait Area: Among other uses this promoter sequence can be useful to improve: Seed development-seed abortion, seed size, seed germination Utility: Improve germination rate, seed longevity and nutrition quality

Construct: YPOl 15 Promoter Candidate I.D: 13148237 cDNA I.D: 12561466 Tl lines expressing (T2 seed): SR.00692-03,-11,-12

Sequence (SEQ ID NO: 63):

ATGATGAACATTCTACATATATAATTATTATGTTTAAGCACTTAGACAGCATAAATT CTTTCTAATT ATATAAATCTAACCTTGTTACATTGTACATCTATAAATTACTTGAAGAAATAACGAGTTC TATTTCT TTTTAAAAATTAAAAATACTATACCATATCTCAGTGATTAAGTTGAACCAAAAGGTACGG AGGAGA AACAAGCATTTGATTCTTCCTTATTTTATTTTATTCATCTCTCACTAATGATGGTGGAGA AAAAAAG AAAATACCTAACAAACAAATATATATTGTCATACAAAAATATTTCTATATTTTTAGTTAA TTAGTTT ATATTCCTCACTTTTCAGGGCTTATATAAGAAAGTGAGCAAACACAAATCAAAATGCAGC AGCAAA TACTATCATCACCCATCTCCTTAGTTCTATTTTATAATTCCTCTTCTTTTTGTTCATAGC TTTGTAATT ATAGTCTTATTTCTCTTTAAGGCTCAATAAGAGGAGGTACTATTACTACACTTCTCTCTA CTTTTACT TGTATTTTAGCATTAAAATCCTAAAATCCGTTTTAAATTCAAAAATAAACTTAGAGATGT TTAATCT CGATTCGGTTTTTCGGCTTTAGGAGAATAATTATATGAAATTAGTATGGATATCTTTACT AGTTTCC ATTCAAATGATTCTGATTTCAATCTAATACTCTCACTCTTTAATTAAACTATATGTAGTG TAATTTC ACACTGTTAAATTTCTACCATGTCATGTATATTAGAGTTGCATAGAAAATTGTAAAACAT CCATTTG AATTCGAATGAAACAAAATGTTTTAAAATAAAATTTTGGTTTTTAAAAGAAAAATCTAAA ACTGAA TTATATCGTTTAACCAAGTTGTAAAAGTCATAAAACGTAGTATCTTGTAAATCGCTCTTC CACGGTC CAAATAGACTTCTAGTAATAAACAAGTAAAACTAATTTTGGTTTCTTACTAATT

Promoter Expression Report # 72 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Stem (H)stomata Hypocotyl (L)epidermis Cotyledon (L)petiole Rosette Leaf (L)epidermis, (L)petiole Primary Root (M) epidermis, (L)root hairs Observed expression pattern: Tl mature: High expression was seen in stem guard cells, but not observed in any other tissues. T2 seedling: High epidermal expression was detected in the hypocotyl at the petiole junction then decreasing in gradient fashion toward proximal organs. Low expression was seen throughout the root epidermis. Expected expression pattern: COB RNA is upregulated in cells in the root elongation zone; expressed in siliques, stem, roots using northerns, less so in flowers Selection Criteria: Literature; COBRA encodes a putative GPI-anchored protein, which is polarly localized and necessary for oriented cell expansion in Arabidopsis. Genes Dev. 2001 May 1;15(9):1115-27. Gene: putative glycosylphosphatidylinositol (GPI) -anchored protein GenBank: NM_125485 Arabidopsis thaliana phytochelatin synthetase - like protein (At5g60920) mRNA, complete cds gi]30697444]reflNM_125485.21[30697444] Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewBin4-HAPl-GFP Marker Type: GFP- Generation Screened: X Tl Mature X T2 Seedling T2 Mature T3 Seedling

T2 Mature Plant Expression Plants expressing / Plants screened Event-01: no data Event-02: no data D Scheduled D T2 Mature tissue expressions similar to Tl expression data (data not shown). D T2 Mature tissue expression (if different expression pattern). Expression detected

T3 Seedling Expression Seedlings expressing / Seedlings screened Event-01: no data Event-02: no data D Scheduled D T3 Seedling tissue expressions similar to T2 seedling expression (data not shown). D T2 Mature tissue expression (if different expression pattern). GFP Expression Detected

Promoter utility Trait - Sub-trait Area: Among other uses this promoter sequence can be useful to improve: PG&D - stress tolerance, nutrient uptake

Construct: YP0133 Promoter Candidate LD: 11768667 cDNA LD: 13601536 Tl lines expressing (T2 seed): SR00722-01,-11,-12

Sequence (SEQ ID NO: 64):

gtcgattggtaTTAGTCCAATAACTTTCACAGTTTTACTATAATATAATATTGAAAG TATGGTTAATTGAC AATAGGGTATTTCCACTGATAATCAGTCACCAGTAATAATAATTTGTTGGGACTAGAATC AATCAC TTGAGTTTTAAATTGATATATGCCTAGATGGTTGTTAGGACCAATATTCCTAAACAATAC CAAATTT TAATATAGAAATGTAAAATAATTCGCCGACACATTATTTCGTTATTGACATTGAATATTT CAATGAT ATCAACTCTGTTTATTTACATCTATTTAGTATAATTTCTTGAGCAACAAAAAAAGTAGTT TTAAAGT TATTTTCCCTAGATTTTTCTATTGTAAAAGCCTATATATCACAAcaaaaattaACCATTT TCTCTTTTGGC AAGTTTAAAATTTTTATAATGAATATACCattttaaaaaaaaaatTATCCAAACTAAATT AACCATTTTCTCTT TTGGCAACAAAAATTAACCatttaaaaaaaaaaaaaaaaaaaaagTTTAAAATTTTTATA ACAACAGAAAGTAATA TTATCCAAACTATGAATAATTAAAAAATCTGTCCACATACGATCATATATTTCTCTCCAC CGACAGC CGAAAACACTGTCAATGGCCCACGTTCCTCTAAAAGCTGTCGTCTTCGGTAATATTTTCC GGTAATA AACTAACTTCCGATCACAATTACACAAAAGCCCCTTTCTCGTTTATAATCATAGGCTATG ATTCATA GCAAACTTACAGAGTTGGTTATTAAGAAGTCAAAAATACAGATTCCTTAAAATATTTTTT TTCTGTT CATATATTTTTTATCGAGAAACAATTActtaaacATAAGAAGCAAGACAAGAATTAATGT TCTTATTAA CATGATGCTAATATAATCGGAAAACAAAATCAAATCATGATAGAAGGGTAAAAtggtcaa atcatcgttcccg gctcaaattttactaaaacagccactagccagccagatcc

Promoter Expression Report # 73 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Silique (L)ovule Ovule (H)embryo sac, (H)gametophyte, (M)embryo sac Primary Root (H)epidermis, (H)endodermis, (H)root hairs Lateral root (H)epidermis Observed expression pattern: Tl mature: Images F-I show expression throughout the developing female gametophyte. Onset of expression is at approximately fertilization stage and decreases shortly after fertilization while expression continues in degenerated synergid cells. No expression was detected in embryo or endosperm beyond division of central cell and normal onset of endosperm cellularization. Expression in gametophyte cells of abnormal unfertilized mature ovules. Expression in penetrated synergid cells appears degenerated at mycropylar pole which indicate pollination has occurred, however, fertilization of egg cell and central cell did not occur resulting in lack of embryo and endosperm. T2 seedling: High expression in epidermal cells throughout root. Expected expression pattern: carpel walls Selection Criteria: Arabidopsis Gene: GDSL-motif lipase/hydrolase-like protein GenBank: NM_123959 Arabidopsis thaliana GDSL-motif lipase/hydrolase protein (At5g45910) mRNA, complete cds gill8422608|refjNM 123959.1|[18422608] Source Promoter Organism: Arabidopsis thaliana WS Vector: Marker Type: GFP-ER Generation Screened: X Tl Mature X T2 Seedling T2 Mature T3 Seedling

Tl Mature Plant Expression Organs/Tissues screened Events Screened: n=3 Events Expressing: n=2 GFP Expression Detected DFlower □ pedicel D receptacle D nectary D sepal Q petal D filament D anther D pollen D carpel D style D papillae D vascular D epidermis D stomata G trichome X Silique D stigma D style D carpel D septum D placentae D transmitting tissue D vascular D epidermis □ stomata D abscission zone L ovule X Ovule Pre-fertilization: D inner integument D outer integument H embryo sac funiculus chalaza micropyle H gametophyte Post-fertilization: G zygote M embryo sac Q inner integument D outer integument □ seed coat D richoblas Q chalaza G micropyle G early endosperm G mature endosperm G embryo D Embryo Q suspensor D preglobular D globular G heart G torpedo late mature Q provascular 0 hypophysis 0 radicle 0 cotyledons G hypocotyl D Stem epidermis cortex vascular G xylem G phloem O pith 0 stomata O trichome DLeaf petiole mesophyll Q vascular epidermis D trichome G richoblas Q stomata G stipule G margin X in the Egg apparatus (Ea ) of the ovule and fertilized ovule X in the Antipodal cells (Ap), Central cell (Cc), Synergid cell (Sn), Egg sac (Es), Egg apparatus (Ea ) and Micropyle (Mp) of the ovule X in the Antipodal cells (Ap), Central cell (Cc), Synergid cell (Sn) and Egg apparatus (Ea ) of the fertilized ovule Chalaza (Ch),), Egg cell (Ec), Funiculus (Fn), X in the Antipodal cells (Ap), Central cell (Cc), Cv, Sc and Micropyle (Mp) in an abnormal ovule I Chalaza (Ch), Egg apparatus w/ synergid cells (Ea), Egg sac (Es), Funiculus (Fn)

T2 Mature Plant Expression Plants expressing / Plants screened Event-01: no data Event-02: no data D Scheduled D T2 Mature tissue expressions similar to Tl expression data (data not shown). D T2 Mature tissue expression (if different expression pattern). Expression detected

T3 Seedling Expression Seedlings expressing / Seedlings screened Event-01: no data Event-02: no data D Scheduled D T3 Seedling tissue expressions similar to T2 seedling expression (data not shown). D T2 Mature tissue expression (if different expression pattern). GFP Expression Detected

Promoter utility Trait - Sub-trait Area: Among other uses this promoter sequence can be useful to improve: Seed Development - endosperm production, seed size, seed abortion Utility: Increase seed size and yield Construct: YPO 137 Promoter LD: 11768695 cDNA LD: 13603975 Tl lines expressing (T2 seed): SR00840-10,-l l

Sequence (SEQ ID NO: 65):

GTGGCACATGCTGAAACCCCGAGCATCTCTCCGGAAGACACGCGTCGTTCGCTCCAA AGAAAACAGT CACAGCTGCCGGAGAATCTCCGCCGTCTTCTTCTGCCACCGGAAAAACTCTCTCCACCAC TTTCAGT GCCCACCTCGTGTTATATCCACTGTATCCTCGTAGCACCATATCAGCCTAATAAAATTTT ATGTATC AAATTTTAAGACATAGCCGAAACTACACTATACTAGACAATAATAATATGATTTGTTTCC TGAAAA ATTATGGTTTCATGAGAAACATTAATCATCTATAAAACAAATTAGCTATGGCATCGAAGA GTTATC AATCAAAACTGATGAATCTTTACTTAATATATACAACATATCTTTACCTTGCGGCGGAGA AGATCGGC GAGAGAAGCACCCCAGCCACCGTCACTAAAGGATTCTTCAGTGATGGAATCACCAAAGAG AAAAA CCTTCCGTCTCATCATCTTCCACACAATCTTCTTGAGAAAATCTGAGAGATAAGAAAGGT GTAGTG GTTTTGCTGAAGTGATCGTGTTTGATTTAGTAAAGAAATGCTTTATTTATTGTTGGGGGA AACATAA ATAAATAAAGTAAAAGTGGATGCACTAAATGCTTTCACCCACTAATCACCGACCTTTCAT GGTTTA TTGTGAAATACACTCATAGATAGACATACAATACCTTATGTACGTAAATAACATTTTATT TGTCGAC ACTTATGTAAGTAACGCATAGATTATTTTCTATGTGATTGCCACTCTCAGACTCTCAGTT TCAACCA ATAATAACAATAACTACAACAACATTAATCATAAACATATGCTCTGGTTTACAATTAAAG CTTAGATT AAGAAACTGTAACAACGTTACAGAAAAAAAATGTTATTTACGTTTTGTAAGATTAGTCTC TAGAAT- CATCACCGTTTTTTATATATTAATGATTCTTTCTTATATataaaacctttctcgaaatac ccatgaaa

Promoter Expression Report # 74 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Flower (H)pedicel, (H)sepal, (H)carpel, (H)style, (H)epidermis Silique (H)style, (H)carpel, (M)septum, (H)epidermis, (H)cortex Stem (H)epidermis, (H)cortex Leaf (H)mesophyll, (L)vascular, (H)epidermis Hypocotyl (H) epidermis, (H)cortex Cotyledon (H)mesophyll, (H)epidermis Rosette Leaf (H) epidermis Observed expression pattern: Tl mature: High expression throughout vegetative tissues. No expression observed in reproductive organs, ovules or embryos. Not expressed in guard cells. T2 seedling: High expression throughout vegetative tissues. No expression observed in root. Expected expression pattern: N induced, source tissue, cDNA AFLP, 24h N induced Selection Criteria: Arabidopsis AFLP-nitrogen Gene: ATH 131206 Arabidopsis thaliana mRNA for microbody NAD- dependent malate dehydrogenase GenBank: NM 121003 Arabidopsis thaliana malate dehydrogenase, glyoxysomal (At5g09660) mRNA, complete cds gi|30682786|reflNM 121003.2|[30682786] Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewbin4-HAP 1 -GFP Marker Type: GFP-ER Generation Screened: X Tl Mature XT2 Seedling T2 Mature T3 Seedling

Tl Mature Plant Expression Organs/Tissues screened Events Screened: n=3 Events Expressing: n=3 GFP Expression Detected

T2 Mature Plant Expression Plants expressing / Plants screened Event-01: no data Event-02: no data D Scheduled D T2 Mature tissue expressions similar to Tl expression data (data not shown). D T2 Mature tissue expression (if different expression pattern). Expression detected

T3 Seedling Expression Seedlings expressing / Seedlings screened Event-01: no data Event-02: no data D Scheduled D T3 Seedling tissue expressions similar to T2 seedling expression (data not shown). D T2 Mature tissue expression (if different expression pattern). GFP Expression Detected

Promoter utility Trait - Sub-trait Area: Among other uses, this promoter sequence can be useful to improve: PGD- Size, biomass, source strength, yield, stress tolerance

Construct: YPO 144 Promoter Candidate LD: 13148149 cDNA LD: 12705120 Tl lines expressing (T2 seed): SR00730-10,-π,-12

Sequence (SEQ ID NO: 66):

aaacGTTGCAAGATTATTGATTGTGAgaaagaGTGCTCAAGGTAGTACTGATTTCTG TAAAGCTCACGGT GGTGGGAAACGATGTTCTTGGGGAGATGGGAAATGTGAgaaaatttgCTAGAGGAAAgaa gcGGTTTATG CGCTGCGCATAACACTATTATGTCTCGGGAGAACAAAGATGGAAGCAAGAGCGGTTTGAT TGGAC CGGGACTCTTTAGTGGCCTTGTTTTTGGCTCTACTTCTGATCATTCTCAGTCTGGAGCTA GCGCTGTC TCTGATTGTACTGATTCTGTTGAACGAATACAGTTTGAGAATAGGCAGAAGAACAAGAAG ATGAT GATACCGATGCAGGTTCTAGTACCTTCATCAATGAAATCTCCAAGTAATTCACATGAAGG AGAAAC AAACATCTATGACTTCATGGTTCCGGAGGAGAGAGTTCACGGCGGTGGGCTAGTAATGTC TTTACT TGGTGGCTCCATTGATCGAAACTGAAAGCCATTTATGGTAAAAGTGTCACATTCTCAGCA AAAACC TGTGTAAAGCTGTAAAATGTGTGGGAATCTCCGAATCTGTTTGTAGCCGGTTACGTTATG CTGGAT CAAAAACTCAAGATTTGTTGGATATTGTTATGCTGGATCGGTGGTGAAACCACTTCCCGC ITTGCTA AATAAATAAACGTTTTTGTTTTATAATCTTTTTCACTAAACGGCAGTATGGGCCTTTAGT GGGCTTC CTTTAAGCGACCAATACAATCGTCGCACCGGAATCTACTACCATTTATAGGTTTATTCAT GTAAAAC CTCGGAAAATTTGAGAGCCACAACGGTCAAGAGACAAAAACAACTTGAAGATAAAGGGAT AAGG AAGGCTTCCTACATGATGGACAACATTTCTTTCCACACAAATTCTCATAATAAAAATCTT ATAATAC AAATACTT ACGTCATAATCATTCAATCTAGTCCCCATGTTTT Aaggtcctgtttcttgtctgalacaaat

Promoter Expression Report # 75 Promoter Tested In: Arάbidopsis thaliana, WS ecotype Spatial expression summary: Flower (L)stomata Silique (L)ovule Ovule Post-fertilization: (M)embryo Embryo (M)torpedo, (M)late, (M)mature, (H)radicle, (M)cotyledons Stem (L)stomata Observed expression pattern: Tl mature: High preferential expression was detected in the radicle and root cap of developing embryos. GFP levels increased in cotyledons as the embryo reached maturity. Low guard cell expression was detected in flowers and stems. T2 seedlings: No expression was detected. Expected expression pattern: Induced during embryogenesis Selection Criteria: Literature; bZIP transcription factors in Arabidopsis. Trends Plant Sci. 2002 Mar;7(3): 106-11. Review. Gene: AtBZIP67, bZIP protein, At3g44460 GenBank: NM 114314 Arabidopsis thaliana bZIP protein (At3g44460) mRNA, complete cds gi|30692145]ref|NM. 114314.2|[30692145] Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewbin4-HAPl-GFP Marker Type: GFP-ER Generation Screened: X Tl Mature X T2 Seedling T2 Mature T3 Seedling

T2 Mature Plant Expression Plants expressing / Plants screened Event-01: no data Event-02: no data D Scheduled D T2 Mature tissue expressions similar to Tl expression data (data not shown). D T2 Mature tissue expression (if different expression pattern). Expression detected

T3 Seedling Expression Seedlings expressing / Seedlings screened Event-01: no data Event-02: no data D Scheduled D T3 Seedling tissue expressions similar to T2 seedling expression (data not shown). D T2 Mature tissue expression (if different expression pattern). GFP Expression Detected

P r rrrrooommmooottteeerrr u u utttiiillliiitttyyy Trait - Sub-trait Area: Among other uses, this promoter sequence can be useful to improve: Seed development-seed size, seed composition, root development

Construct: YPO 143 Promoter Candidate LD: 11768669 cDNA LD: 13607229 Tl lines expressing (T2 seed): SR00729-10,-! 1

Sequence (SEQ ID NO: 67):

atACAACAGATGGCAGATATCGAGTTAAAtacgtGAATCAGCCGTTACGATATTTTAAAA CTAGAAAAT TATTTAAAAATATTGCAAAATACCATTTAATTTCATTGTTCATAAAAAAAAGAAattcaa aaacTTAAAA ACTGATTCAAAAATTTGGATTAattctcattaACAGTCTTCAACACTACAACAACATGTT TCTAATTTATTT TATATTTTAATAATTAAACAATATATACGTCTGCACATTGTTGCTCCGACATAATCTAGT ATAAAAA TAGTTGCAGCATATGTGAAAAGCAAGCAGCATTTatcacTCAATActtttaATTTTatct gTTGTATGTATTAA GGTTTTGTAGCTTTAAGAAAACGCTTATAATATAAAATAACTTCtaaaagATATTTCATG CGTATACAA TAAATATTTGTGAAAAAACATTTCGAAAACGTGTAcaatatATAAACTATTGTGTTATCT TTTGACATT CAAACAAATGTTGACAATGTAATTTTATCCATGATATGATTGGCCAATTAGCTGCGAGGT AAAAAT CCGTATACGAGTAAAAGTAAGATAAAATTTCGCAAGAAGATTTTTAGCAGGAAATCTAAG ACAAG TGTCATGAACGTGTCAATCAACAAACGAAAAGGAGAATTATAGAATCCAGATTCGACGTA CCACA TTAATAAATATCAAAACATTTTATGTTATTTTATTTTTGCTCTGGCAGTTACACTCTTTT TCATTGCT CCAATAAAAAAatcacTCGCATGCATGCATATATATACACCATAGTAAACTCCGCCTCTT CTTCATTTT AAAAGTATCAGTTTACACTGACACAATCCTTAACTATTTTCCTTTGTTCTTCTTCATCTT TATTACAC ATTTTTTTCAAGGTAACAAATAATCTTTTTAAGTCACTTTTATACTCTTTAAATCTTAGA TTGATATA TGAATGCATGTTAATATTtcaagatttataggtctaccaaac Promoter Expression Report # 76 3- 'Pl::'. Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Ovule Post-fertilization: (H)embryo Embryo (L)radicle, (H)cotyledons Primary Root (H)epidermis, (H) richoblasts, (H)atrichoblast, (H)root hairs Observed expression pattern: Tl mature: High preferential expression in cotyledons of developing embryo with low expression in radicle of mature embryos. T2 seedling: High expression in epidermal cells near root transition zone decreasing toward root tip observed in one of two events screened (I. YP0156-10 T2 seedling expression). The second event showed expression in lateral and primary root meristematic cells but not epidermal (YP0156- 13 T2 seedling expression). Additional events screened showed epidermal expression patterns similar to line -10, however lateral and primary root expression in these lines remains inconclusive. Expected expression pattern: Induced during embryogenesis Selection Criteria: Literature Gene: AtBZIP 12 GenBank: NM 180010 Arabidopsis thaliana bZIP family transcription factor (At2g41070) mRNA, complete cds gi|30688516]ref|NM 180010.1|[30688516] Source Promoter Organism: Arabidopsis thaliana WS Vector: Newbin4-HAP1-GFP Marker Type: GFP-ER Generation Screened: X Tl Mature X T2 Seedling D T2 Mature D T3 Seedling

T2 Seedling Expression Tissues Screened Events Screened: n=4 Events Expressing: n=4 Seedlings expressing / Seedlings screened Event-01: Line -10 3/3 Event-02: Line -13 3/3 *shows different expression pattern. Event-03: Line -11 6/6 Event-04: Line -12 616 D Scheduled GFP Expression Detected

I. YP0156-10 T2 seedling expression. X in the root X in the Epidermis (Ep) and Root hair (Rh) of the root Addition events screened shown to have similar expression pattern as line -10.

II. YP0156-13 T2 seedling expression. X in the lateral root primordial and lateral root tip X in the root tip Line -13 shows different pattern.

T2 Mature Plant Expression Plants expressing / Plants screened Event-01: no data Event-02: no data □ Scheduled D T2 Mature tissue expressions similar to Tl expression data (data not shown). D T2 Mature tissue expression (if different expression pattern). Expression detected

T3 Seedling Expression Seedlings expressing / Seedlings screened Event-01: no data Event-02: no data D Scheduled D T3 Seedling tissue expressions similar to T2 seedling expression (data not shown). D T2 Mature tissue expression (if different expression pattern). GFP Expression Detected

Promoter utility Trait - Sub-trait Area: Among other use, this promoter sequence can be useful to improve: PGD-C/N partitioning, amino acid transport

Construct: YPO 156 Promoter Candidate I.D: 11768688 cDNA LD: 12323871 Tl lines expressing (T2 seed): SR.00810-10,-11,-12,-13

Sequence (SEQ ID NO: 68): ttggtttgCATTGTGAAGATTTGTATT AACTATAGAACATTGAATTGATGGTGTTAAGTTCTTACACAAG CGTGCTTCTCGGTTTGAACTGTTTCTTTTGTATGTTGAATCAGAGCTTAGTTTATAGGAA CCAGAGT ATCTACTTAGTCATTCTctgatGCTAAGTGCTAAGGTTCTACCTAGTTGCCCTCTAGGCC CTTATGTTAT TGATAACTTATGAAGCTATTTGAACACTTGATTCTTAGGAGACCTAAGTTGGTACAGCCA GATAGA GTGTATGTTCTTGTTCTCTATGTGACAGGATCAAGCTGCCACACATAGTTCAAGGGTATG CTCTGTG TGGGTTTGCTCAGATTGAGGACAAATCTATACAAGGAAGTAGAGTCTTTGACATTTTGAT GTTGTA TGATAAGAAGAAGAAAGGAGAGTAATAAAGAAAGAGAAAAGGGAAACAGAAACACGTGGG AGA ACATCCCAAAGAGGAAGCACACGCGGATCTTCATGCAAAGCTCCCCGATTCTCCCATGTG GTCCCT TTCTCCCTTTGTCCCCCTCCTCTTTCTTCTTTTCTCATTTTACTCCTTTTTTTACCATTA TACAACGAA TCTTTTTTATCATAATTTTTTGGTTTTGGTTTATTTTCCAATAACACTTTCTTGGTTACT TCCCATTCT CACTTTTTCATATAAGAAACTCACTTTGGGAAACTTATGTTTGAGAATGACAAGTCTTTT TAGAGAA AGTGATGTAACAAATCTAAAGTGATTATATAATAACCTTGCACAATGTTTTTGATTTTTT GTAAGAT TCGAATATTAGGTTTATTATTCGTAGGGAATAAACTTACTTTCAAAAGCGTTCATAAGTT AATACTT TCATATATGATCATAAGTACGGACACTATTGTTTTTTGTTTGTTTGTGTTTATTCTAAAA GAAAGTA GCTTTT AATTGAAATGTCCtcggaGGCACAGTTTAAAGTTCGAGTgtaacagtttctaaggca

Promoter Expression Report # 77 [[Jf r% Pf Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Flower (H)pedicel, (H)receptacle, (H)sepal, (H)petal, (L)filament, (H)vascular (H)epidertnis Stem (H)epidermis, (H)cortex Hypocotyl (H)epidermis, (H)cortex, (H)vascular Cotyledon (H)vascular, (H)epidermis, (H)hydathode Rosette Leaf (H)vascular, (H)epidermis, (H)petiole, (H)primordia Primary Root (H)epidermis, (H)vascular Observed expression pattern: Tl mature: High expression in epidermal and vascular tissues in floral organs and inflorescence meristem. Expressed in stem epidermis and cortex. Not detected in mesophyll cells. T2 seedling: Very high expression levels epidermal and vascular tissues of seedling. Medium expression in cortical tissues in lower hypocotyl decreasing toward apex. In root, preferentially expressed in epidermal non-root hair producing atrichoblast cells and vascular tissues. Not detected in mesophyll cells or shoot apical meristem and rosette leaf richoblas. Expected expression pattern: Roots Selection Criteria: Ceres Microarray 5-lOX up. Unique Arab. Clones + Yiwen's corn root tips only (2.5X and up) Gene: protein kinase homolog GenBank: NM_124535 Arabidopsis thaliana leucine-rich. repeat transmembrane protein kinase, putative (At5g51560) mRNA, complete cds gil306960671ref|NM 124535.2|[30696067] Source Promoter Organism: Arabidopsis thaliana WS Vector: Newbin4-HAP1-GFP Marker Type: GFP-ER Generation Screened: X Tl Mature X T2 Seedling D T2 Mature D T3 Seedling

Tl Mature Plant Expression Organs/Tissues screened Events Screened: n=4 Events Expressing: n=2 GFP Expression Detected X Flower H pedicel H receptacle D nectary H sepal H petal L filament D anther D pollen D carpel D style D papillae H vascular H epidermis D stomata

T2 Mature Plant Expression Plants expressing / Plants screened Event-01: no data Event-02: no data D Scheduled D T2 Mature tissue expressions similar to Tl expression data (data not shown). D T2 Mature tissue expression (if different expression pattern). Expression detected T3 Seedling Expression Seedlings expressing / Seedlings screened Event-01: no data Event-02: no data D Scheduled D T3 Seedling tissue expressions similar to T2 seedling expression (data not shown). D T2 Mature tissue expression (if different expression pattern). GFP Expression Detected

Promoter utility Trait - Sub-trait Area: Among other uses, this promoter sequence can be useful to improve: PGD-size, growth rate, yield, stress tolerance

Construct: YP0158 Promoter Candidate LD: 11768620 cDNA I.D: 13602983 Tl lines expressing (T2 seed): SR00842-12,-13

Sequence (SEQ ID NO: 69):

ttattagattaatagattgcatTGCATTGCTTGTGCTTTCAATTTACAAATTGTCTC CCAACTCCATCGACACATCT CTTTTTGTGTATATAAGATTCAGACTTGTTATATTTTTTTTATAAATATGTTATTAGCAT CTTAAGTT AAATTGATTTTTTATATCTGCATTAAGGATTACACGACTATATTTGCGATTGTGTGTTGG TTAAAAT ATAATTTAGGATTGTCTTTAACTACATTTAGGATTATATGACTATATTTGGTTAAATATA AAATCTag ctgTGATTATTAGTATtcaaaaatAAGTAGCCTAACCAATTAAAACAACGGCTATTGGGG CAAATTAGAA CATTTTAGTGTGTCCAAAATATAATGGTCATTAGGTCATATTCCTCCTAGCTTCATCGCA GCATAAT TGAATGATTGCCTTATTTAGAAGAGCTTTTCCACTTTCCCAAAATCTAGGTGGGATCTTT TTGTTTT GACCTTCATTTTTCTTGTTTACCATTTTTAGCTAAATTATTTACGATTACAAAAGATATC AAAAGTT GGATCATAATACAATTTATAGACTTACTGTAGAAAATTCGTATGTACAAGTACAACAAAT TCTTCA TAATAAATTTTGAAAATTCTATTACAAATGTTGTAAGAAATAGAATTTGAAATATATATA AACTAA GGAGAAAAAAAAAGAGAACATGCATTGCTCTAGTCAGAGTGGACCAACATCAACGAGATA AGATA ACATAAAAACCAACTCACCATAACTAAAAACATCCCAAGAGATCCAACGATTCATATCAA ACACA AAAACATCGAACGATCAGATTTAAACCATCTCTGGTATCTCCAAAACACAAACActtttt tttttctTTTGTC TGAATGGAACAAAAGCATGCGACATCTCTGTGTCTTTATCTTCTCTCTCCTCTTCTTGAA AAACTGA ACCTTTAATTCTTTCTTCACAtctcctttagctttctgaagctgcta

Promoter Expression Report # 79 | • Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Flower (M)pedicel, (L)receptacle, (L)nectary, (H)sepal, (H)filament, (M)carpel, (M)epidermis Silique (H)carpel, (H)placentae, (H)epidermis, (M)ovule Ovule Pre-fertilization: (L)inner integument, (H)funiculus Post-fertilization: (H)inner integument, (H) embryo Embryo (H)globular, (H)heart, (H)torpedo, (H)late, (H)mature, (H)hypophysis, (H)radicle, (H)cotyledons Stem (L)cortex, (H)vascular Hypocotyl (H)epidermis, (H)cortex, (L)vascular Cotyledon (L)epidermis, (L)petiole Primary Root (H)vascular, (H)pericycle, (H)cap Lateral root (H)initials, (L)vascular, (H)cap Observed expression pattern: Tl mature: Expression was detected throughout the epidermal and cortical cells in the inflorescence meristem with preferential expression in vascular tissues. Low expression existed in the shoot apical meristem with highest expression in developing floral and sepal richoblas. High expression was seen throughout the reproductive tissues in the ovary of deyeloping prefertilized flowers with high specific expression in the inner integuments of pre- and post- fertilized ovules. GFP expression observed from globular to mature embryo development with preferential expression in root meristemic tissues also observed in T2 seedlings root. High expression throughout stem tissue. T2 seedling: High expression was seen in the epidermal and cortical tissues of hypocotyl. Expression was observed throughout the vasculature and root, and lateral root tips. Expected expression pattern: N induced, source tissue Selection Criteria: Gene: "putative serine/threonine protein kinase" GenBank: NM 119436 Arabidopsis thaliana protein kinase, gi[30689505|refjNM 119436.2l[30689505] Source Promoter Organism: Arabidopsis thaliana WS Vector: Newbin4-HAP1-GFP Marker Type: GFP-ER Generation Screened: X Tl Mature X T2 Seedling D T2 Mature D T3 Seedling

T2 Seedling Expression Tissues Screened Events Screened: n=2 Events Expressing: n=2 Seedlings expressing / Seedlings screened Event-01: 1/3 Event-02: 1/3 D Scheduled GFP Expression Detected X Hypocotyl H epidermis H cortex L vascular D xylem D phloem □ stomata X Cotyledon D mesophyll D vascular L epidermis D margin D stomata D hydathode L petiole D Rosette Leaf D mesophyll D vascular D epidermis D trichome O petiole D richoblas D stomata D stipule D margin X Primary Root D epidermis D richoblasts D atrichoblast D cortex D endodermis H vascular D xylem D phloem H pericycle D quiescent D columella H cap D root hairs

X Lateral root D epidermis D trichoblast D atrichoblast G cortex D endodermis H initials D flanking cells L vascular H cap D- Shoot apical meristem D SAM D epidermis X in the Epidermis (Ep), of the hypocotyl X in the Lateral root (Lr) and Vasculature (Vs) of the root X in the lateral root tip X in the Pericycle (Pr) and Vasculature (Vs) of the lateral root initial X in the lateral root primordial X in the Root apical meristem (RAM) of the root tip

T2 Mature Plant Expression Plants expressing / Plants screened Event-01: no data Event-02: no data □ Scheduled D T2 Mature tissue expressions similar to Tl expression data (data not shown). D T2 Mature tissue expression (if different expression pattern). Expression detected

T3 Seedling Expression Seedlings expressing / Seedlings screened Event-01: no data Event-02: no data D Scheduled D T3 Seedling tissue expressions similar to T2 seedling expression (data not shown). D T2 Mature tissue expression (if different expression pattern). GFP Expression Detected

Promoter utility Trait - Sub-trait Area: Among other uses, this promoter sequence can be useful to improve: PGD-shoot development and architecture, branch number, flower number, seed number and yield, source strength, growth rate, size, root development and architecture Construct: YPO 164 Promoter Candidate I.D: cDNA LD: 12385295 Tl lines expressing (T2 seed): SR.00813-10,-11,-13,-15

Sequence (SEQ ID NO: 70):

gtcgattggcaTTACTAGAGTTTTAGATTTCACAGAATTCTTTTCTTTGTTTGGTCT GTTCGTCCTCTACTG TGTTTTTCTTTTGTTTTGGCTATTTTGCCGTCTGTTAAAAAATTGGGGTTTTCCGATAAT GTATtgtatA TGCCTGAATTATGGAAATATCACTAAAAACACTTTTTTGCACTTTTGTATATATTGCAAT TAAGATT TAATTTTTCTATTTTGAAGACTTTTTGTTAAAAGAGAGATTTGCTAACTCAAAATTACCG GAGTTAA TAAGTTCGTTCTCATCAGATTAGCAAAACATTCATCTTTAAAGAAATCTGTTTATCTCAC GTAAAAT TCTACTAATTGGCAGCAgcacaCTACTTTAATGGGCCTATTACTATTTCAATATGCACTG AAAACTTCA TTGGACTGTCCAATAACATTATAACATTATTAAAACTAAACTACATTTGTATTTGGGTTA CAATTTT ATAAAGAGTAAGACTCAAGATACTAATTGGGCTGCAGACAACTTTAAAAGCCTTACTACA AAAAT CAAAACCATTCTTAATTAACTAACAACCAGGTAAAAATCTCAGTACAATATTTACACTAA AAATAG GTGCCACCCACTATGCAAAAGTTTAGGGCCACGAACAAAAAAACCTGTAAATTGTTATGT CTTCAC AATATGTGTTTTAATACACATGAATTTTAGCTGcgGTTAATGTAAATTTTGTAGTT AAATT AAGGCT AAACAATCTCAAATATAAATTGGTCAGGTCCACAGACAACAGCCTGGCAAGTGGCAAGCA CTAAA AATTCGACCGTTATTTTTTGCCCCTTTTTTTAATATTTCGAAATTGTATCTTTTAGTTTT ATTTTAAAG CTTTTTAGCCCGCtcctcCTCCGCTCCACCTTTAATTTTTTCACCAATTGGATTTGGATC TGTCAAAAAT ATTGGCCTCTTTCTCTCTTTCTCTCTTGCTCTCTTTCTTTGTTGGGTTga

Promoter Expression Report # 80 >J S f Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Silique (H)ovule Ovule Post-fertilization: (EQinner integument, (H)outer integument, (H)seed coat Observed expression pattern: Tl mature: Ovules from within the same silique show variable expression levels in the outer and inner integuments of ovules. Series of optical sections of ovule show high expression in the inner integument and no or low expression in the outer integument. Optical sections of ovules also show opposite expression pattern. Series stacked sections of ovules show an equal level of expression. Expression levels off as ovules mature. Expression in late endosperm is detected. Detailed observation of endosperm required. T2 seedling: No expression was detected. Expected expression pattern: Expressed in ovules and different parts of seeds Selection Criteria: Greater than 5Ox up in pi ovule microarray Gene: AGAMOUS-LIKE MADS BOX PROTEIN GenBank: NM 115740 Arabidopsis thaliana MADS-box protein (At3g58780) mRNA, complete cds gi|30694874|ref|NM_115740.2|[30694874] Source Promoter Organism: Arabidopsis thaliana WS Vector: Newbin4-HAP1-GFP Marker Type: GFP-ER Generation Screened: X Tl Mature X T2 Seedling DT2 Mature DT3 Seedling

Tl Mature Plant Expression Organs/Tissues screened Events Screened: n=3 Events Expressing: n=2 GFP Expression Detected D Flower Dpedicel D receptacle D nectary D sepal Dpetal D filament D anther Dpollen D carpel D style Dpapillae D vascular D epidermis Dstomata Dtrichome

T2 Mature Plant Expression Plants expressing / Plants screened Event-01: no data Event-02: no data D Scheduled D T2 Mature tissue expressions similar to Tl expression data (data not shown). D T2 Mature tissue expression (if different expression pattern). Expression detected

T3 Seedling Expression Seedlings expressing / Seedlings screened Event-01: no data Event-02: no data D Scheduled D T3 Seedling tissue expressions similar to T2 seedling expression (data not shown). D T2 Mature tissue expression (if different expression pattern). GFP Expression Detected

Promoter utility Trait - Sub-trait Area: Among other uses, this promoter sequence can be useful to improve: Seed development- seed size, seed abortion, endosperm production, seed composition

Construct: YPOl 19 Promoter Candidate I.D: 13148175 cDNA LD: 13607852 Tl lines expressing (T2 seed : SR.00712-01,-11,-15

Sequence (SEQ ID NO: 71):

taCCAAAAATAAGGAGTTtccaaaagATGGTTCTGATGAGAAACAGAGCCCATCCCT CTCCTTTTCCCCT TCCCATGAAAGAAATCGGATGGTCCTCCTTCAATGTCCTCCACCTACTCTTCTCTTCTTT CTTTTTTT CTTTCTTATTATTAACCATTTAATTAATTTCCCCTTCAATTTCAGTTTCTAGTTCTGTAA AAAGAAAA TACACATCTCACTTATAGATATCCATATCTATTTATATGCATGTATAGAGAATAAAAAAG TGTGAG TTTCTAGGTATGTTGAGTATGTGCTGTTTGGACAATTGTTAGATGATCTGTCCATTTTTT TCTTTTTT CTTCTGTGTATAAATATATTTGAGCACAAAGAAAAACTAATAACCTTCTGTTTTCAGCAA GTAGGG TCTTATAACCTTCAAAGAAATATTCCTTCAATTGAAAACCCATAAACCAAAATAGATATT ACAAAA GGAAAGAGAGATATTTTCAAGAACAACATAATTAGAAAAGCAGAAGCAGCAGTTAAGTGG TACTG AGATAAATGATATAGTTTCTCTTCAAGAACAGTTTCTCATTACCCACCTTCTCCTTTTTG CTGATCTA TCGTAATCTTGAGAACTCAGGTAAGGTTGTGAATATTATGCACCATTCATTAACCCTAAA AATAAG AGATTTAAAATAAATGTTTCTTCTTTCTCTGATTCTTGTGTAACCAATTCATGGGTTTGA TATGTTTC TTGGTTATTGCTTATCAACAAAGAGATTTGATCATTATAAAGTAGATTAATAACTCTTAA ACACAC AAAGTTTCTTTATTTTTTAGTTACATCCCTAATTCTAGACCAGAACATGGATTTGATCTA TTTCTTGG TTATGTATTCTTGATCAGGAAAAGGGATTTGATCATCAAGATTAGCCTTCTCTCTCTCTC TCTAGAT ATCTTTCTTGAATTTAGAAATCTTTATTTAATTATTTGGTGATGTCATatataggatcaa

Promoter Expression Report # 82 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Silique (H)style, (H)septum, (H)epidermis, (H)cortex Primary Root (L)epidermis, (H)endodermis, (L)pericycle, (L)vascular Observed expression pattern: Tl mature: High expression was seen in the style of silique which extends down to the carpel - septum connective region. T2 seedling: High expression was detected throughout the endodermis and pericycle cells of root. Low epidermal and vascular expression was detected. Expected expression pattern: 2-3x induced in senescing leaves versus non-senescing leaves Selection Criteria: Literature Gene: 12-OXO-PHYTODIENOIC ACID- 10, 11 -REDUCTASE GeπBank: NM 106319 Arabidopsis thaliana 12-oxophytodienoate reductase (OPR2) (Atlg76690) mRNA, complete cds gi|30699191|ref]NM 106319.2|[30699191] Source Promoter Organism: Arabidopsis thaliana WS Vector: Newbin4-HAP1-GFP Marker Type: GFP-ER Generation Screened: X Tl Mature XT2 Seedling D T2 Mature D T3 Seedling

T2 Mature Plant Expression Plants expressing / Plants screened Event-01: no data Event-02: no data G Scheduled Q T2 Mature tissue expressions similar to Tl expression data (data not shown). Q T2 Mature tissue expression (if different expression pattern).

T3 Seedling Expression Seedlings expressing / Seedlings screened Event-01: no data Event-02: no data D Scheduled D T3 Seedling tissue expressions similar to T2 seedling expression (data not shown). D T2 Mature tissue expression (if different expression pattern). GFP Expression Detected

Promoter utility Trait - Sub-trait Area: Among other uses, this promoter sequence can be useful to improve: PGD-root development and architecture Nutrients-nutrient uptake

Construct: YP0171 Promoter Candidate LD: 3148156 cDNA I.D: 13619728 (0Id ID 4954325) Tl lines expressing (T2 seed): SR00846-01,-02

Sequence (SEQ ID NO: 72):

aatGAAAACAATGGGAGAAGTACATGCTTGTCCTCACACACTAATGCCGATGAGGAA AgcctttaAGGG GACTTTTATCTCCGCAGGAGGTTTCACGAGGGAAGATGGGAATGAGGCTGTGTCAAAGGG AAGAA CTGATTTGGTGGCTTATGGTCGATGGTTTCTAGCCAACCCGGACCTGCCAAAGAGGTTCC AAGTGG ATGCACCGCTGAATAAGTACGATAGACCAACGTTTTACACTTCTGATCCAGTCGTCGGTT ACACCG ATTACCCTTTCCTCGAATCAACAGCTTAAAATTgttatcAATAATGTAATGTAGTGTGTT TCCCTTATAT AAGATGTAATAAGTTTCTGGCTTTTCATTTATACTTTTTAAGTTTAAGTCATAAAACCTT CACAAAA ATTTCCACGGACACATTATCACAAAAGCGCTTTCTAGAGACCAACATAACTTAACTTGAT TGTTGA TTTCTGTTTGATGTGATCATGCCGCAATCCAGTGTGTTCTCATGATGCTATCTTCCCTCC TTTCACAT GCTGCACAGAACAAAACAGAGCATTTTCCTCCCAACTATACCTAATTTTTTTTGGTCGGT GGTCAAA GTTATACATCGGAAGAATCTGTTGAAATCATATTGAGGCCCCTCATTTGTTATGTTATGT AATCTCC AATGGATCAAAAGTAGAATCCCAACTGTAGAAGATGATACTATCATGCTAGGTAGAAGAC AGCAT GGAATATGGGGTAAGTTCAAAGTGGTTACACTCTAATGTCGTCTCAACGAATACGTCTTT ATGAAG AAATAAAAAAATCTAAGTGGTTGGATGCGTCATCAATGACGACCACGTCGTTAAAGACAG AAAGA AACACTTGCGTTCCTGATTCTCTGATCAATCAATGTGTATAAATATGTTCGGATATGTCC ATTATCT TACGCAATCTTGAAAAGTGTTTTTGAGAGAAATATAGGTTTTACAAAATCCACCgttgtg aattca

Promoter Expression Report # 84 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Flower (L)stomata Silique (H)placentae, (H)vascular, (H)ovule, (H)funiculus Ovule Pre-fertilization: (H)funiculus Post-fertilization: (H)funiculus, (H)chalaza, (H)micropyle, (H) embryo Embryo (H)suspensor, (H)preglobular, (H)globular, (H)heart, (H)torpedo, (H)hypophysis (H)radicle Stem (L)cortex, (H)vascular Leaf (H)mesophyll, (H)vascular, (H)epidermis Hypocotyl (H)epidermis, (H)vascular Cotyledon (H)mesophyll, (H)vascular, (H) epidermis, (H)hydathode, (H)petiole Rosette Leaf (H)mesophyll, (H)vascular, (H)epidermis, (H)petiole Primary Root (H)vascular, (H)pericycle Lateral root (H)initials Observed expression pattern: Tl mature: High expression was seen in reproductive tissues throughout the developing embryo. Expression was detected early in suspensor and hyphsis of the early globular embryo and later in throughout the maturing embryo. Mature embryos beyond the torpedo stage were not screened. Ovule expression was restricted to the outer and inner integuments at the chalaza and micropyle region. High expression was observed throughout all tissues in the leaf and vasculature of the stem. Low guard cell expression was seen in the flowers and stem. T2 seedling: High epidermal, vascular, and mesophyll expression existed in all arial seedling tissues. High expression extends throughout the stele of the seedling root. Expression was observed only in the lateral root initials and primordial. Expected expression pattern: High in siliques Selection Criteria: Arabidopsis microarray Gene: Fe-superoxide dismutase GenBank: NM179110 Arabidopsis thaliana iron superoxide dismutase (FSDl) (At4g25100) mRNA, complete cds gi]30686767|reflNM 179110.1|[30686767] Source Promoter Organism: Arabidopsis thaliana WS Vector: Newbin4-HAP1-GFP Marker Type: GFP-ER Generation Screened: X Tl Mature X T2 Seedling D T2 Mature D T3 Seedling

X in the Suspensor (Su) of the early globular embryo X in the Suspensor (Su), Embryo (Em), Hypocotyl (Hy) and Outer integument (Oi) of the late globular embryo X in the Suspensor (Su) and Embryo (Em) of the early heart stage embryo X in the mid-heart, late heart and torpedo stage embryo X in the leaf Epidermis (Ep), Mesophyll (Me) and Vasculature (Vs) X in the Cortex (Cr), Epidermis (Ep) and Guard cell (Gc) of the stem

T2 Mature Plant Expression Plants expressing / Plants screened Event-01: no data Event-02: no data D Scheduled D T2 Mature tissue expressions similar to Tl expression data (data not shown). D T2 Mature tissue expression (if different expression pattern). Expression detected

T3 Seedling Expression Seedlings expressing / Seedlings screened Event-01: no data Event-02: no data D Scheduled D T3 Seedling tissue expressions similar to T2 seedling expression (data not shown). D T2 Mature tissue expression (if different expression pattern). GFP Expression Detected

Promoter utility Trait-Sub-trait Area: Among other uses, this promoter sequence can be useful to improve: Seed development-seed abortion, seed size, seed composition

Construct: YP0188 Promoter Candidate LD: 13148244 cDNA LD: 13504706 Tl lines expressing (T2 seed): SR00851-01,-02,-03

Sequence (SEQ ID NO: 73):

gattggtaTGAAATTTCGGAGACCAACAAAAAAAACTTTATTGAGCTTGGAGTGAAG CTATATATATGG GGCAAGATCATAATATGTTTATATCGGCCTTTtcgttaACTGAAAATAATAGTTTTGAGA AATATATCA AATGGTAAACAGACATCATCTTTGAAAAATACCATCAATGAAGTTAATATTGTTATTGGC ATATGG TTTACCCATCTTAATTTTAATGCAACCAAACAAACAAGAAACAAAAACTGTATAAGATAC AAGGTG TTTTACGATTTTCCGTCTTAAAACCGAAATATTTTTGTTCCTACGACTTTAAACGGACTT TGCTTAA GTTGTGTGCATGTAAGCTCGTCGtccctcgattgtcaTCAACATTCACCAATATCAGCCT CTATCACACGAGt gaaggtggtgattcgGCTTAATGAAAACagagaaatatttcaatatgattcctattaaat tttaaatcttttttctcaatctctagattttcattaaaagcatcatg atttttttccactatgttcatatatctctatcacagttttaggtacattgtagaaattgg ataagatacgtcatacgtctaacatgaatttggtctagcaaggaaggtttgagat aataagtgaaaagaaaacacaagataataaattataatttataaatgctttatagtattg aaaaataagatgaLLLLLLLLLLUllaataccggatTGGCTGATCCA CTTATGATGACTCAAATGTTATTAAGTTTCAAGACAATTTATGATGacacaaatcacaAT GAGTCAATAGT AgccacgAAGCCAGAAAAAAAAAATGTACTACAAAAAGATAATGATAGTACAAAATGATA CGTCGTA CTGCCACATGTACGACACAACTCGATTACCAAAAAGCAGAGCCATCCAACCATAAAACTC AAAAC ACACAGATTCCACTGGCGTGTGCTCTCctcacTTCACTCGTCCTTGAAACTtgaggtact ga

Promoter Expression Report # 85 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Flower (L)receptacle, (L)sepal, (L)petal, (L)vascular, (L)epidermis Primary Root (L)epidermis, (L)root hairs Observed expression pattern: Tl mature: Low expression was seen in the vasculature of developing floral buds of inflorescence meristem. Expression was not detected in mature flowers. T2 seedling: Low expression was seen throughout root epidermal cells. Expected expression pattern: cotyledon/Leaf/sepal/petal richoblas, not in SAM Selection Criteria: Gene: "expressed protein with a DUF607 Pfam domain" GenBank: NM _ 126063 Arabidopsis thaliana expressed protein (At5g66650) mRNA, complete cds gi|30698243MNM 126063.2|[306982431 Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewBin4-HAPl-GFP Marker Type: GFP-ER Generation Screened: X Tl Mature X T2 Seedling T2 Mature T3 Seedling

Tl Mature Plant Expression Organs/Tissues screened Events Screened: n=3 Events Expressing: n=2

T2 Mature Plant Expression Plants expressing / Plants screened Event-01: no data Event-02: no data D Scheduled D T2 Mature tissue expressions similar to Tl expression data (data not shown). D T2 Mature tissue expression (if different expression pattern). Expression detected

T3 Seedling Expression Seedlings expressing / Seedlings screened Event-01: no data Event-02: no data D Scheduled D T3 Seedling tissue expressions similar to T2 seedling expression (data not shown). D T2 Mature tissue expression (if different expression pattern). GFP Expression Detected

Promoter utility Trait - Sub-trait Area: Among other uses, this promoter sequence can be useful to improve: PGD-nutrient uptake and transport, seedling establishment, stress tolerance, drought tolerance Utility: Express insecticidal proteins, increase transport and uptake of nitrogen compounds

Construct: YP0258 Promoter Candidate LD: 11768747 cDNA I.D: 12688873 Tl lines expressing (T2 seed): SR01015-02,-03

Sequence (SEQ ID NO: 74):

taAATAACTTACTTAAATTTATATT AAAACCGTTTACATCTGATATAATTTAAAAGGTTTTAGACGAA TTAGTATTAGTGAACCATTATCAAGATGTATTTATTACAAAATCTATAATAATAAAAACA AAACTG GGAAAGCAATGGGAAGAAGATCTGATTGGCTTtggaagACTTCGCGTGTTAATAAGATTT TTCTCAAA AAAGAAAGATCGGACGGCAATGCGTGGCAAGTCGCTTATGACGTCGGAAGCTTAGCTTGC CACGT GTACGGTCATCACCATTTAGGTGACCGATATAATGTATTTCCCGTGACTTGACAATCTAA TCTCCCA ATTTATCGTGCTCCAGTGTCTCAAGAGAGAACTTACTCTATTGGTTACCCAAAACTCACA TAAAAC GTGGATTTTATATTTTTTTTTATCAAATAATTTGATGTGCAATATAAACATATATGTATT ATTATCTT GCATGTTTAAAAAATGCTAACTTAAATTTTTCTTCAATGATTGAGATTTTTCATACACAT CCATCGA GTTCACACTTATGATTAAACAAAGTGTATTGTTACACTAATAACGGTTCTAAGTTGTTTC AATACGT TTATGATAACGGTTCTATACTAATACTAGTATTCATACGTTTATGAAACTCGTTAATTAT ATATTTA ACTTTGTTTAGCTGTATCATAACAAGCGTTTTAAGAAATAATTTAATAAAAAATGAgaaa acgaTAAGC GACGCCTTATCGCCTATGTGTAATTATCGTGTCAAAATATCGTGATAAGGAAGCTGTTTC AGAGGA GCCTTCTCGTTTGTTGCGTCGTTGCTCTGAGCCAACAACGCTAATATAAAAGGAAGCTCA AGTCTCT CTGTTTTAATCTCGGACCAATATACAAAACCGTGTGTTCTTCTCTGTATCTTATTAAATC AAAACCA ATTTTGTTCTTCTTCTTTGATTCTTTTTTCCTTCATTTTTtaacgtatcttgagagatcg ac

Promoter Expression Report # 86 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Flower (L)sepal, (M)petal, (L)filament, (H)carpel, (H)vascular, (H)epidermis Silique (H)carpel, (H)cortex, (H)placentae, (H)vascular, (H)epidermis, (H)stomata, (H)ovule Ovule Pre-fertilization: (H)outer integument, (H)funiculus Post-fertilization: (H)outer integument, (H)seed coat, (H)embryo Embryo (H)globular, (H)heart, (H)hypophysis Stem (L)vascular Leaf (L)mesophyll Cotyledon (L)epidermis Rosette Leaf (H)mesophyll, (H)epidermis, (H)trichome, (H) richoblas Primary Root (H)epidermis, (H)cap, (H)root hairs Observed expression pattern: Tl mature: High expression was restricted to the sepals of developing floral buds in inflorescence meristem and throughout all organs in mature flowers. High expression was observed in vascular tissues of reproductive tissues and floral organs. High vascular expression in placenta extended through to developing pre-fertilized ovules. Expression in ovules was restricted to outer integument, seed coat, and embryonic root meristem cell. Expression in leaf was specific to abaxial spongy mesophyll cells. T2 seedling: High expression was seen in root meristem and epidermal cells of primary root and mesophyll cells of rosette leaf. Expression in seedling rosette leaf also appears to be abaxial specific). Low expression was seen in epidermal cells at margin cotyledons. Expected expression pattern: SAM leaf richoblas, lmm leaf margin Selection Criteria: CSHL gene trap GT 1040 Gene: T28I19.100; expressed protein GenBank: NM 11.9943 Arabidopsis thaliana expressed protein (At4g37820) mRNA, complete cds gi|30691596]ref1NM .119943.2| [30691596] Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewBin4-HAPl-GFP Marker Type: GFP-ER Generation Screened: X Tl Mature X T2 Seedling D T2 Mature DT3 Seedling

I X in the Epidermis (Ep) and Vasculature (Vs) of the stem

T2 Mature Plant Expression Plants expressing / Plants screened Event-01: no data Event-02: no data G Scheduled D T2 Mature tissue expressions similar to Tl expression data (data not shown). Q T2 Mature tissue expression (if different expression pattern). Expression detected

T3 Seedling Expression Seedlings expressing / Seedlings screened Event-01: no data Event-02: no data G Scheduled Q T3 Seedling tissue expressions similar to T2 seedling expression (data not shown). G T2 Mature tissue expression (if different expression pattern). GFP Expression Detected

Promoter utility Trait - Sub-trait Area: Among other uses, this promoter sequence can be useful to improve: PGD-stress tolerance, seedling development and establishment, nutrient transport Seed development- embryo development and composition, seed composition Construct: YP0265 Promoter Candidate LD: 11768754 cDNA LD: 13604752 Tl lines expressing (T2 seed): SRO 1022-04,-05,-06

Sequence (SEQ ID NO: 75):

taAATAACTTACTT AAATTTATATTAAAACCGTTTACATCTGATATAATTTAAAAGGTTTTAGACGAA TTAGTATTAGTGAACCATTATCAAGATGTATTTATTACAAAATCTATAATAATAAAAACA AAACTG GGAAAGCAATGGGAAGAAGATCTGATTGGCTTtggaagACTTCGCGTGTTAATAAGATTT TTCTCAAA AAAGAAAGATCGGACGGCAATGCGTGGCAAGTCGCTTATGACGTCGGAAGCTTAGCTTGC CACGT GTACGGTCATCACCATTTAGGTGACCGATATAATGTATTTCCCGTGACTTGACAATCTAA TCTCCCA ATTTATCGTGCTCCAGTGTCTCAAGAGAGAACTTACTCTATTGGTTACCCAAAACTCACA TAAAAC GTGGATTTTATATTTTTTTTTATCAAATAATTTGATGTGCAATATAAACATATATGTATT ATTATCTT GCATGTTTAAAAAATGCTAACTTAAATTTTTCTTCAATGATTGAGATTTTTCATACACAT CCATCGA GTTCACACTTATGATTAAACAAAGTGTATTGTTACACTAATAACGGTTCTAAGTTGTTTC AATACGT TTATGATAACGGTTCTATACTAATACTAGTATTCATACGTTTATGAAACTCGTTAATTAT ATATTTA ACTTTGTTTAGCTGTATCATAACAAGCGTTTTAAGAAATAATTTAATAAAAAATGAgaaa acgaTAAGC GACGCCTTATCGCCTATGTGTAATTATCGTGTCAAAATATCGTGATAAGGAAGCTGTTTC AGAGGA GCCTTCTCGTTTGTTGCGTCGTTGCTCTGAGCCAACAACGCTAATATAAAAGGAAGCTCA AGTCTCT CTGTTTTAATCTCGGACCAATATACAAAACCGTGTGTTCTTCTCTGTATCTTATTAAATC AAAACCA ATTTTGTTCTTCTTCTTTGATTCTTTTTTCCTTCATTTTTtaacgtatcttgagagatcg ac

Tl Mature Plant Expression Organs/Tissues screened Events Screened: n= 2 Events Expressing: n= 2 GFP Expression Detected X Flower L pedicel L receptacle Dnectary L sepal L petal L filament Danther Dpollen D carpel D style Dpapillae D vascular L epidermis Dstomata Dtrichome Dsilique D Silique Dstigma Dstyle Dcarpel Dseptum Dplacentae Dtransmitting tissue Dvascular D epidermis Dstomata D abscission zone D ovule D Ovule Pre-fertilization: Dinner integument Douter integument Dembryo sack Dfuniculus Dchalaza Dmicropyle Dgametophyte Post-fertilization: Dzygote D embryo sack Dinner integument Douter integument Dendothelium Dseed coat Dprimordia Dchalaza Dmicropyle Dearly endosperm Dmature endosperm Dembryo D Embryo Dsuspensor Dpreglobular D globular D heart D torpedo D late Dmature Dprovascular Dhypophysis Dradicle Dcotyledons Dhypocotyl X Stem Depidermis Dcortex L vascular Dxylem Dphloem Dpith Dstomata Dtrichome X Leaf Dpetiole D mesophyll M vascular Depidermis Dtrichome Dprimordia Dstomata D stipule Dmargin L rib D Shoot apical meristem D Shoot apical meristem D Flower primordium X in the flower bud and inflorescence meristem X in the Cortex (Cr) of the Receptacle (Re) and the Vasculature (Vs) of the Sepal (Se) of the flower X in the Vasculature (Vs) of the leaf and mid-vein rib

T2 Seedling Expression Tissues Screened Events Screened: n= 2 Events Expressing: n= 2 Seedlings expressing / Seedlings screened Event-01: 2/2 Event-02: 2/2 D Scheduled GFP Expression Detected X Hypocotyl L epidermis L cortex H vascular Dxylem Dphloem Dstomata X Cotyledon L mesophyll Dvascular L epidermis Dmargin Dstomata D hydathode X Rosette Leaf L mesophyll L vascular Lepidermis Dtrichome H petiole Dprimordia Dstomata Dstipule Dmargin Dhydathode X Primary Root Depidermis Otrichoblast Datrichoblast Dcortex Dendodermis H vascular Dxylem Dphloem Dpericycle Dquiescent Dcolumella Droot cap Droot hairs D Lateral root Depidermis Dtrichoblast Datrichoblast Dcortex Dendodermis D initials D flanking cells Dvascular D lateral root cap D Shoot apical meristem D Shoot apical meristem

X in the Vasculature (Vs) and Cortex (Cr) of the hypocotyl X in the Vasculature (Vs) and Guard cells (Gc) of the leaf X in the Epidermis (Ep) and Mesophyll (Me) of the rosette leaf X in the Vasculature (Vs) of the petiole X in the Vasculature (Vs) of the root transition zone Promoter utility Trait Area: Sub-trait Area: Utility: Translocation, seed fill. Improved loading of phloem, increased source capacity. Increased seed yield. Cotyledon angle, improved seedling survival. Notes: Extensive studies on plant signaling molecules over the past decade indicate that plant cell-to- cell communication, as is the case with animal systems, makes use of small peptide signals and specific receptors. To date, four peptide-ligand-receptor pairs have been identified and shown to be involved in a variety of processes. CLVl is one of them. Matsubayashi . Ligand-receptor pairs in plant peptide signaling. J Cell Sci. 2003 Oct 1;116(Pt 19):3863-70.

Construct: YP0071 Promoter candidate I.D: 11768674 cDNA LD: 12721583 (OCKHAM3-C) Lines expressing: YP0071-01. YP0071-02

Sequence (SEQ ID NO: 76):

ataggccctacttctaattaaagcccatttacttctctccttgtcttcttattcctc ttttctccccatcacgtgacgacgatgctataaacgccgtcggattatataactggtg ccgttgacaagacggcgacag'aagaaagaaagaagaaaccacaggctctagggaacgta acgttatgtcctgtctatagcatttataacggtcagatcaacgccg tttagataaagatctgtcaatgttaaagaagagatgcatctctacaccgttaaatttaaa acgccgtgaacctcttatctattgatttttgtttgatgaagccaaaacaaatc gtgtcagaagacttatcagagaagaagaaaacgacgacgttcccgtttctccatgtctaa taagtgtagtagtggcggctactaaaaactctaaagtttgactccagta aaactgcctttctagtgtaattccagtgattttagagtttgaatagtgtgtgaccaaatt tgaaagtacaatctcagcaatattattgatcactcgttataaaagaatcgaat gtaaaaatagccaatgagagactgagacgtatgtgtttgaccataagtcgtatagtttgt atctatctacctgcaagatcagcagatggttctctgatcaattgtacctta attatcttttattttcgtaaaatttctctattcacaaatgataaatctacttaagacagt aaccataacaagatttacaagataatttgaaaaatgaacacataaaagtattttg gcgcattatttttaataataacaatatttatgtaaagtcacataaaagtatatattcgct cacaaagtcttacggtatttagaacagtagtaccacatcgattctcttcatcttc ttcttcataatatgccattgttcatgtctctgtgtcctatcgcataacactcacgctatc ttattattttctctcgctctttctcactga

cds gi]30684674]reflNM 121396.2][30684674] Source Promoter Organism:Arabidopsis thaliana WS Vector: Newbin4-HAP1-GFP Marker Type: GFP-ER Generation Screened: X Tl Mature X T2 Seedling DT2 Mature DT3 Seedling

Tl Mature Plant Expression Organs/Tissues screened Events Screened: n=3 Events Expressing: n=3 D No GFP Expression Detected X Flower H pedicel H receptacle H nectary Hsepal H petal Hfilament Hanther Dpollen Hcaipel Hstyle H stigma D vascular H epidermis Dstomata Dtrichome H silique X Silique H stigma H style H carpel H septum ϋplacentae Dtransmitting tissue □ vascular H epidermis CU stomata Habscission zone D ovule D Ovule Pre-fertilization: Dinner integument D outer integument D embryo sac D funiculus Dchalaza Dmicropyle Dgametophyte Post- fertilization: D zygote Dinner integument D outer integument D endothelium D seed coat Dprimordia Dchalaza Dmicropyle Dearly endosperm Dmature endosperm D embryo D Embryo □ suspensor Dpreglobular Oglobular Dheart Dtorpedo Dlate Dmature Dprovascular D hypophysis D radicle D cotyledons Dhypocotyl D Stem Depidermis Dcortex Dvascular Dxylem Dphloem Dpith Dstomata Dtrichome

D Leaf Gpetiole Dmesophyll Dvascular Depidermis Dtrichome Dprimordia Dstomata D stipule Dmargin X in the Anther (An), Petal (Pe) and Sepal (Se) of the inflorescence meristem X in the Anther (An), Silique (Si), Ovule (Ov), Placenta (PI), Petal (Pe) and Sepal (Se) of the flower X (weak) in the leaf X in sepals of floral primordial

X in the Epidermis (Ep) and Vasculature (Vs) of the root X in the Epidermis (Ep) and Vasculature (Vs) of the cotyledon petiole X in the Epidermis (Ep), Mesophyll (Me) and Vasculature (Vs) of the rosette leaf X in the Epidermis (Ep), Vasculature (Vs) of the Cortex (Cr), Endothelium (Ed), Pericycle (Pr) and Root hair (Rh) of the root X in the primary root cap

T2 Mature Plant Expression Plants expressing / Plants screened Event-01: no data Event-02: no data D Scheduled D T2 Mature tissue expressions similar to Tl expression data (data not shown). D T2 Mature tissue expression (if different- expression pattern). Expression detected

T3 Seedling Expression Seedlings expressing / Seedlings screened Event-01: no data Event-02: no data D Scheduled D T3 Seedling tissue expressions similar to T2 seedling expression (data not shown). D T2 Mature tissue expression (if different expression pattern). GFP Expression Detected

Promoter utility Trait - Sub-trait Area: Among other uses, this promoter sequence can be useful to improve: PGD-shoot development and architecture, branched inflorescence number, flower number, seed yield, stress tolerance

Construct: YP0214 Promoter Candidate LD: 11768733 cDNA I.D: 12705056 (Old IDs: 12371852, 7080526) Tl lines expressing (T2 seed): SR00546-01,-02,-03

Sequence (SEQ ID NO: 77):

ccagtcgattggcgcctcgcatgCCTATCATATTTAACCGTCAATAATGGATTTGGCGGT TTTGGTAGGCCGGGT CAACCGGATTAAAAGAAAACGGTTTGGAGTCCTTCCTTGCAATTGAATTTTCACACATTC GGGTTTT GTGATTTCTCTGTCATAATGGGCCCGGCACATATGGTTCATAACCCATGTGGGCCTATGG TATAATT TTTCCAATTAAAACTATTGTTAGGTCGATAAAACAAAAAACAATAAAAACGAGTGGAATA CACAT ACCAAAAAGAATGTGATGAACATTAGTAATTTTATTTTGATGGTTAATGAAAAACAAAAT AAATGC ATCTTGGCATCTTCCGTTGGAAAGCGCAAATAGGGCAGATTTTCAGACAGATATCACTAT GATGGG GGGtgagaGAAAGAAAACGAGGCGTACCTAATGTAACACTACTTAATTAGTCGTTAGTTA TAGGACT TTTTTTTTGTTTGGGCCTAGTTATAGGATCATAAGGTAAAAATGAAGAATGAATATTAGA TTAGTA GGAGCTAATGATGGAGTTAAGTATGCACGTGTAAGAACTGGGAAGTGAAACCTCCTGTAT GGTGA AGAAACTATACAACAAAGCCCTTTGTTGGTGTATACGTATTAATTTTTATTCTTTTATCA CAAGCGA TACgtatcttaAGACATAATAAATATATATCTTACTCATAATAAATATCTTAAGATATAT ATACAGTATA CACCTGTATATATATAATAAATAGGCATATAGTAGAAATTAATATGAGTTGTTGTTGTTG CAAATA TATAAATCAATCAAAAGATTTAAAACCCACCATTCAATCTTGGTAAGTAACGAAAAAAAA GGGAA GCAAGAAGAACCACAGAAAAGGGGGCTAACAACTAGACACGTAGATCTTCATCTGCCCGT CCATC TAACCTACCACACTCTCATCTTCTTTTTCCCGTGTCAGTTTGTTATATAAGCTCTCACTC TCCGgtatattt ccccattgcactgga

Promoter Expression Report # 101 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Flower H pedicel, H receptacle Silique H placentae Stem H epidermis H cortex H vascular, L pith Hypocotyl H epidermis, H vascular Cotyledon H mesophyll, H vascular, H epidermis, H hydathode Rosette Leaf H mesophyll, H vascular, H epidermis, Hprimordia Primary Root H epidermis, H cortex, H vascular Lateral root H epidermis, H cortex Observed expression pattern: Tl mature: High expression in epidermis and cortical cells of stem and pedicles near inflorescence shoot apex. Weakens near floral organs except in the placenta where GFP is also highly expressed. Not expressed in ovules or embryos. High GFP expression in vasculature of stem.T2 seedling: High expression throughout leaves and epidermis of hypocotyl. No expression observed in ground tissues of hypocotyl. High epidermal, cortex and vascular expression in root. Expected expression pattern: Enzyme located in chloroplasts, >4 fold high in seedlings Selection Criteria: Ceres Arabidopsis microarray Gene: product="DEF (CLAl) protein" CLAl (for "cloroplastos alterados', or "altered chloroplasts') CLAl encodes 1-deoxy-d-xylulose 5-phosphate synthase, which catalyses the first step of the non-mevalonate isoprenoid biosynthetic pathway. GenBank: NM 117647 Arabidopsis thaliana DEF (CLAl) protein (At4gl5560) mRNA, complete cds gi|30683316|reflNM 117647.2][30683316] Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewbin4-H AP 1 -GFP Marker Type: GFP-ER Generation Screened: X Tl Mature X T2 Seedling DT2 Mature DT3 Seedling

Tl Mature Plant Expression Organs/Tissues screened Events Screened: n=4 Events Expressing: n=4 GFP Expression Detected X Flower H pedicel H receptacle Dnectary Dsepal Dpetal Dfilament Danther Dpollen Dcarpel π style Gpapillae Dvascular Depidermis Dstomata Dtrichome D silique X Silique D stigma D style Dcarpel D septum H placentae D transmitting tissue G vascular Depidermis Dstomata D abscission zone D ovule D Ovule Pre-fertilization: Dinner integument D outer integument D embryo sack Dfuniculus Dchalaza Dmicropyle Dgametophyte Post-fertilization: D zygote D embryo sack Dinner integument D outer integument D endothelium Dseed coat Dprimordia Dchalaza Dmicropyle Dearly endosperm Dmature endosperm D embryo O Embryo Dsuspensor Dpreglobular Dglobular Dheart Dtorpedo Dlate Dmature Dprovascular Dhypophysis Dradicle Dcotyledons Dhypocotyl X Stem H epidermis H cortex H vascular Dxylem Dphloem L pith Dstomata Dtrichome

T2 Seedling Expression Tissues Screened Events Screened: n= 2 Events Expressing: n= 2 Seedlings expressing / Seedlings screened Event-01: 2/2 Event-02: 2/2 D Scheduled GFP Expression Detected X Hypocotyl H epidermis D cortex H vascular Dxylem D phloem Dstomata X Cotyledon H mesophyll H vascular H epidermis D margin Dstomata H hydathode X Rosette Leaf H mesophyll H vascular H epidermis Dtrichome Dpetiole H primordia Dstomata D stipule Dmargin Dhydathode X Primary Root H epidermis Dtrichoblast Datrichoblast H cortex Dendodermis H vascular Dxylem Dphloem Dpericycle D quiescent D columella D root cap D root hairs X Lateral root H epidermis Dtrichoblast Datrichoblast H cortex Dendodermis Dinitials Dflanking cells Dvascular Dlateral root cap D Shoot apical meristem D Shoot apical meristem X in the Epidermis (Ep), Mesophyll (Me), Hydathode (Hd) and Vasculature (Vs) of the cotyledon X in the Epidermis (Ep), Mesophyll (Me) and Vasculature (Vs) of the rosette leaf (Cr), Lateral root (Lr), Rosette leaf (Rl),

Promoter utility Utility: Increased photosynthetic capacity and source capacity. Larger plants. Altered plant morphology. Altered plant metabolism. Increased seed Loading and seed yield. Notes: CLAl encodes 1-deoxy-d-xylulose 5-ρhosphate synthase, which catalyses the first step of the non-mevalonate isoprenoid biosynthetic pathway. Crowell DN, Packard CE, Pierson CA, Giner JL, Downes BP, Chary SN. Identification of an allele of CLAl associated with variegation in Arabidopsis thaliana. Physiol Plant. 2003 May;118(l):29-37.

Construct: YP0216 Promoter candidate LD: 13148171 cDNA LD: 12575820 Lines expressing: YP0216-01, -02, -03, -04

Sequence (SEQ ID NO: 78):

ttttgtttctaatagtttgatgtttatatcaacattattatttacttt^ attaatattartaaattaataaaaataacaaactgtaaaagctattttrttaaaaattrt gtgtaaaacatctaaaaattartct^ gaagataatagtgtgaaattatatatatatatagaaatatataaagtaggatttttttct gtatacaaatatacgittccaaimatcaaaaactgtaaagatiitt ttctttgtcagiacctgctaaacttgttaatttiittattaaaaaaaaatcaa^ ttgcctttatcgtctttggnatgtatgcgtgaatataWatttattttcttttc^ atgtactaacgtatataaataatatcatcaatatctatccaaaacttggatttcatggtt gacgtggcccaaccaaaatctcaagttctctgcggatgacga accatctcaccatctctttttttctctctctittt^^ ccccattttttaaaagtctccatctttatacttcttcaactctccaccaccaccattgtc accaccacatttaaacacacactttcacttgtagtgggattagaa agtgcgrtttattcatttgtittactgtttttgataacctcaaaatttgccta^ ctgagcttcaaaaag

Tl Mature Plant Expression Organs/Tissues screened Events Screened: n=3 Events Expressing: n=2 GFP Expression Detected D Flower Dpedicel Dreceptacle Dnectary Dsepal Dpetal Dfilament Danther Dpollen Dcarpel Dstyle Dpapillae Dvascular Depidermis Dstomata Dtrichome Dsilique D Silique Dstigma Dstyle Dcarpel Dseptum Dplacentae Dtransmitting tissue Dvascular Depidermis Dstomata D abscission zone D ovule X Ovule Pre-fertilization: L primordia L inner integument L outer integument Dembryo sack Dfuniculus Dchalaza Dmicropyle Dgametophyte Post-fertilization: Dzygote H suspensor Dembryo sack Dinner integument Douter integument Dendothelium Dseed coat Dprimordia Dchalaza Dmicropyle Dearly endosperm Dmature endosperm Dembryo D Embryo Dsuspensor Dpreglobular Dglobular Dheart Dtorpedo Dlate Dmature Dprovascular Dhypophysis Dradicle Dcotyledons Dhypocotyl □ Stem Depidermis Dcortex Dvascular Dxylem Dphloem Dpith Dstomata Dtrichome □ Leaf Dpetiole Dmesophyll Dvascular Depidermis Dtrichome Dprimordia Dstomata D stipule D margin □ Shoot apical meristem | D Shoot apical meristem D Flower primordium X in the Megaspore mother cell (Mmc), Outer integument (Oi) and Funiculus (Fn) of the ovule primorida X in the Suspensor cells (Su) of the globular embryo stage in the ovule X in the Suspensor apparatus. After first asymmetrical cell division of zygote, the larger basal cell differentiates into 9 cells See Willemsen, V., et.al. Development. 1998 Feb;125(3):521-31. X in the Suspensor cells (Su) of the heart stage embryo X in the Micopyle (Mp)

Promoter utility Trait Area: SSR Sub-trait Area: Sink strength, apomixis, seed size, embryo size, genetic containment Utility: Better embryo fill, larger embryo and seed. Altered seed composition. Increased seed weight and yield. Better performing seedlings. Seedlings Tolerant to stress. Altered source-sink balance. Control of seed coat development. Increase endosperm production and increase seed fill. Notes: Balasubramanian S. Schneitz K. NOZZLE links proximal-distal and adaxial-abaxial pattern formation during ovule development in Arabidopsis thaliana. Development. 2002 Sep;129(18):4291-

Construct: YP0271 Promoter candidate I.D: 11768757 cDNA LD: 12658070 Lines expressing: YP0271-01,-02

Sequence (SEQ ID NO: 79):

atctdgattttttttatcaggaacaagtaaataaatagctttgagtttttgttttttttc te^ attctegaaactataaatcattatat(^gatdgtttcacaaactaatcatattcgttgca aagtgagaattcgtcc∞cttiBctctte tatt^^tfit^g^fttagtπaaflartrtatatattaa_?_ffi (λaactøtcatactataatgttgaatttcttccacgatccaatgcatggataacaaca tcaatc^ ttgttcdtttcatgaccatattaacatcaaataatgcatttttttcaacgte^^ caagtgcrtaagggaattøtøcatctaaggttaataagactaagaaagtata^ ^££Eg£fo^aaagfa3aaaagfta^33ffltflngt^tragrafatagfar/rtggf t^ agagatacacacacacaaπaπgagagcagaaacaaaacacacacacttaaagc^cgtc tttacctcttccc^crtdctdxfatctaaaaagagttccga

Promoter Expression Report # 103 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Silique L ovule Ovule Post-fertilization: M zygote L embryo sack L embryo Embryo M suspensor L torpedo L radicle Rosette Leaf M mesophyll H epidermis H stomata Primary Root H pericycle Lateral root H initials H flanking cells H primordia Observed expression pattern: Tl mature: High expression throughout mature female gametophyte at fertilization and in embryo from zygote to torpedo stage embryo. Expression in embryo restricted to radicle. Not observed in leaf, however this may coincide with severe yellowing of leaves in plants screened during this time. May have not been detected due to intense autoflorescence of necrotic tissue. T2 seedling: High GFP expression in mesophyll and epidermal cells of rosette leaves. Expression in root is specific to pericycle cells and lateral root primordia. Expected expression pattern: Leaf Selection Criteria: Literature. Leaf-Specific Upregulation of Chloroplast Translocon Genes by a CCT Motif- Containing Protein, CIA 2. Sun CW, Chen LJ, Lin LC, Li HM. Plant Cell. 2001 Sep: 13(9): 2053-2062. PMCID: 139451

Gene: CIA2 GenBank: NM 125100 Arabidopsis thaliana CIA2 (CIA2) (At5g57180) mRNA, complete cds gi|30696839|ref|NM 125100.2|[30696839] Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewbin4-H AP 1 -GFP Marker Type: GFP-ER Generation Screened: XTl Mature XT2 Seedling DT2 Mature DT3 Seedling

Tl Mature Plant Expression Organs/Tissues screened Events Screened: n=3 Events Expressing: n=3 GFP Expression Detected D Flower Dpedicel Dreceptacle Dnectary Dsepal Dpetal Dfilament Danther Gpollen Dcarpel Ostyle Dpapillae Dvascular Gepidermis Dstomata Dtrichome D silique X Silique Dstigma Dstyle Gcarpel Dseptum Dplacentae Qtransmitting tissue Dvascular D epidermis Dstomata D abscission zone L ovule D Ovule Pre-fertilization: Dinner integument D outer integument Dembryo sack D funiculus Dchalaza Dmicropyle D gametophyte Post-fertilization: M zygote L embryo sack Dinner integument D outer integument D endothelium D seed coat Dprimordia Dchalaza Dmicropyle Dearly endosperm Dmature endosperm L embryo X Embryo M suspensor Dpreglobular D globular Dheart L torpedo D late Dmature Dprovascular Dhypophysis L radicle Dcotyledons Dhypocotyl D Stem Depidermis Dcortex Dvascular Dxylem Dphloem Dpith Dstomata Dtrichome D Leaf Dpetiole Dmesophyll Dvascular Depidermis Dtrichome Dprimordia Dstomata D stipule Dmargin D Shoot apical meristem [ Q Shoot apical meristem D Flower primordium X in the Embryo (Em), Embryo sack (Es) and Micropyle (Mp) of the fertilized ovule X in the Embryo proper (Ep) and Suspensor (Su) of the zygote X in the Micropyle (Mp) pole of the ovule X in the fertilized ovule X in the Radicle (Rd) and Root apical meristem (Ram) of the torpedo stage embryo

Promoter utility Trait Area: SSR Sub-trait Area: Embryo size, sink strength, seed size. Utility: Nutrition. Imprint modulation through female, heavier seed, lighter seed, seedless fruits. Increased lateral root growth. More lateral roots, larger lateral roots. Improved drought tolerance. Improved performance in low- nitrogen soil, improved source capacity.

Construct: YP0279 Promoter candidate I.D: 11768839 cDNALD: 12600234 (OCKHAM3-C) Lines expressing: YP00279-01, -02, -03

Sequence (SEQ ID NO: 80): cgctttattetaggtttaacaattgatttttcattattttgttt^^ aaatttac^gattcaaaca<xagtcattgtagattagccaagagttttcagtaacaa aatttaccttata^ tatctctatccataagaaaactcgttttacaaagtaattttaaatcaatacaaaaaagtg a cttcaccattcgctcadctctctctctctctdcgtcttcttcttcccaccactctctctg tttctccacaacttctcttcrtcaaagflaa^ cactatttatttadaaactctcctttgittgtiactaagctcrtcac^ cttctttctgtaaaacctcactcgttggccaatt(rttttggttttcatacacataaatc tcagattccaaatgggtttt<±agctc^ tcccteaagttttgatctttttttcccccttcaattttgtattttctcaccaaataRπa aaaggtttcttcagtgggttttaag aaaaatcttaatttcatcactctcttctcactcacaaaagttcttacaatcttcaaagtt ttggtcttgtttcttttcx;

Promoter Expression Report # 105 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Silique L ovule Ovule M embryo sack Leaf L vascular Hypocotyl L vascular Cotyledon L vascular, M hydathode Primary Root L epidermis, M vascular, M pericycle Observed expression pattern: Tl mature: GFP expression decreasing in female gametophyte. Low expression in leaf vasculature. T2 seedling: Low expression in cotyledon and hypocotyl vasculature. Low expression in root vasculature and pericycle cells. Expected expression pattern: PEG-inducible Selection Criteria: Ceres, Inc. Arabidopsis Expression data Gene: Arabidopsis thaliana mitochondrial carrier protein family GenBank: NM 118590 Arabidopsis thaliana mitochondrial carrier protein family (At4g24570) mRNA, complete cds gi|30686585|reflNM 118590.2|[30686585] Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewbin4-HAPl-GFP Marker Type: GFP-ER Generation Screened: X Tl Mature X T2 Seedling DT2 Mature DT3 Seedling

Tl Mature Plant Expression Organs/Tissues screened Events Screened: n=4 Events Expressing: n=3 GFP Expression Detected D Flower Gpedicel Dreceptacle Dnectary Dsepal Dpetal Dfilament Danther Dpollen Dcarpel Dstyle Dpapillae Dvascular Depidermis Dstomata Dtrichome D silique X Silique Dstigma Gstyle Dcarpel Dseptum Gplacentae Dtransmitting tissue Dvascular Depidermis Dstomata D abscission zone L ovule X Ovule Pre-fertilization: Dinner integument Douter integument M embryo sack D funiculus Dchalaza Dmicropyle D gametophyte Post-fertilization: D zygote G embryo sack Dinner integument Douter integument D endothelium D seed coat Dprimordia Dchalaza Dmicropyle Dearly endosperm Omature endosperm Q embryo D Embryo Dsuspensor Dpreglobular Dglobular Dheart Gtorpedo Dlate Gmature Gprovascular Dhypophysis Dradicle Ocotyledons Dhypocotyl D Stem Depidermis Dcortex Dvascular Dxylem Dphloem Dpith Dstomata Dtrichome X Leaf Dpetiole Dmesophyll L vascular Depidermis Dtrichome Dprimordia

Promoter utility Trait Area: SSR Sub-trait Area: Methylation control, endosperm production, sink strength Utility: Imprint modulation through female, larger (heavier) seeds, smaller (lighter) seeds, seedless fruits. Altered endosperm and seed composition, improved drought tolerance. Improved performance in low-nitrogen soil.

Construct: YP0285 Promoter candidate LD: 11768588 cDNA LD: 13609092 Lines expressing: YP0285-01, -02, -04

Sequence (SEQ ID NO: 81):

gggatøtatatgatagacgattgtatttgcgggacattgagatgttt∞gaaaatagt catca^ actøtatcatttgtaagttgagcgttgaaagaaaatgaaagagtgtagacrtgtagtac gtat^ gaatgaagatttttacctgccatgttgatagagaaaggcaaataaatgtaggggtcgatg tctaacgtaaagactggatcaaccaagagtcctcctcctcg gagtcctcctcgtggaaacttatttcttctccagccaagatctcatctcatctcttcact ctatgaaataiaaaggaatctetggtttttctaaaaactatagtacgtct^^ acaatatfiRaatcaffla^tffgatfiafrttltgBgtRBatfffltaa^ atacacacacactatgagaaatcataaiagagaattttaatgattttgtctaartcatag caacaagtcgctttggccgagtggttaaggcgtgt^ cgcgagagttcgaatdtfcaggcgacgtttcttttgttttcggccataaaggaaaaagc^ tcacacacgcgttttcctactttttgactatttttataaccggcgggtctgacttaatta gggttttctttaaiHatcagacactd^ cgt

Promoter Expression Report # 106 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Flower H vascular Silique H vascular Stem H vascular Leaf H vascular Hypocotyl H vascular Cotyledon H vascular Rosette Leaf H vascular Primary Root H vascular, H pericycle Lateral root Hpericycle H vascular Observed expression pattern: Tl mature: Very high expression in vasculature of flowers, stems, and leaves. Not detected in reproductive tissues in silique.T2 seedling: Very high expression throughout seedling vasculature. Expression in root extending into pericycle cells. Expected expression pattern: Shoot apical meristem Selection Criteria: Greater than 5x down in stm microarray Gene: Leucine-rich repeat transmembrane protein kinase GenBank: NM 118146 Arabidopsis thaliana leucine-rich repeat transmembrane protein kinase, putative (At4g20270) mRNA, complete cds gil306850441ref|NM 118146.2[[30685044I Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewbin4-HAPl-GFP Marker Type: GFP-ER Generation Screened: XTl Mature XT2 Seedling DT2 Mature DT3 Seedling

Tl Mature Plant Expression Organs/Tissues screened Events Screened: n=3 Events Expressing: n=3 GFP Expression Detected X Flower Dpedicel Dreceptacle Dnectary Dsepal Dpetal Dfilament Danther Dpollen D carpel D style Dpapillae H vascular D epidermis Dstomata Dtrichome D silique X Silique D stigma D style D carpel D septum Dplacentae D transmitting tissue H vascular D epidermis Dstomata D abscission zone D ovule D Ovule Pre-fertilization: Dinner integument D outer integument D embryo sack D funiculus Dchalaza Dmicropyle Dgametophyte Post-fertilization: D zygote D embryo sack Dinner integument D outer integument Dendothelium Dseed coat Dprimordia Dchalaza Dmicropyle Dearly endosperm Dmature endosperm D embryo D Embryo Dsuspensor Dpreglobular Dglobular Dheart Dtorpedo Plate Dmature

Promoter utility Trait Area: Nutrition Sub-trait Area: Nitrogen use efficiency Utility: Improved translocation, improved source capacity and seed fill. Heavier seeds. More seeds. Larger siliques. Improved seed yield. Moderate nitrate and/or amino acid transport. Increased transport to floorsink. Notes: LRR kinases play key roles in cell-cell interactions and interactions Between plant and microbial cells. Specific leucine repeats bind Low molecular weight ligands.

Construct: YP0080 Promoter candidate I.D: 11768676 cDNA I.D: 12603755 (OCKHAM3-C) Lines expressing: YP0080 -01, -02, -03

Sequence (SEQ ID NO: 82): aagcggcaatttagtaagaagtattcaatgtatcatttaccaaaagtatHtggttttggg aagagttgttagggatgtattctttctaaacagatgaM taaagacgggatctctogcatcttcactcgggagatatettaaaccgttgattg^ tcatttaaacattagaacatattgtttaacttgttcttciagaaaiBaaactgdaatttc ^ tatgtttatatatatgaaacctaaatcaaattatcccaataattgggagacacaaaagaa aaaaagaaaacaggaaatcaaatcaaaagataaagagaaggtaaaacaaaaggcaa gaagcactaatgtttaaiatttatagttttctccattaaagaaaaagcgatgatgt^^ aacaacaacaaaaaaaaacctaaagdcaat∞aaaagacgaagaatgcattggatadac aacttctttttcacttttctttcgaa^ aataaataaaaaaacgaggcatgaaaataatgaiMcctcttcacttattaagccarica^ gtc^tgatgatgatcttcttcgttccatttctctaaatttttgggattt^^

Promoter Expression Report # 107 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Rosette Leaf L vascular M epidermis Primary Root H epidermis M root hairs Observed expression pattern: Tl mature: No expression observed. Predicted expression in ovule primordium. Possibly missed low expression. T2 seedling: High expression throughout root epidermal cells. Low epidermal and vasculature expression at leaf margins. Expected expression pattern: Integument. Selection Criteria: Arabidopsis public: The BELLI gene encodes a homeodomain protein involved in pattern formation in the Arabidopsis ovule primordium. Gene: ="homeodomain protein, BELLI (BELl)" GenBank: NM 123506 Arabidopsis thaliana homeodomain protein, BELLI (BELl) (At5g41410) mRNA, complete cds gil30693794|reflNM 123506.21[30693794] Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewbin4-HAPl-GFP Marker Type: GFP-ER Generation Screened: XTl Mature XT2 Seedling OT2 Mature DT3 Seedling

I T2 Seedling Expression Tissues Screened Events Screened: n=3 Events Expressing: n=2 Seedlings expressing / Seedlings screened Event-01: 3/6 Event-02: 3/5 O Scheduled GFP Expression Detected □ Hypocotyl Depidermis Dcortex Dvascular Dxylem Dphloem Dstomata D Cotyledon Dmesophyll Dvascular Depidermis Dmargin Dstomata Dhydathode X Rosette Leaf Dmesophyll L vascular M epidermis Dtrichome Dpetiole Dprimordia Dstomata D stipule Dmargin Dhydathode X Primary Root H epidermis Dtrichoblast Datrichoblast Dcortex Dendodermis Dvascular Dxylem Dphloem Dpericycle Dquiescent D columella D root cap M root hairs D Lateral root Depidermis Dtrichoblast Datrichoblast Dcortex Dendodermis D initials D flanking cells Dvascular D lateral root cap D Shoot apical meristem D Shoot apical meristem X in the Epidermis (Ep) of the root transition zone, the root and the cotyledon X in the Vasculature (Vs) of the cotyledon

Promoter utility Trait Area: Nutrition Sub-trait Area: Low nitrogen use efficiency. Utility: Improve ion uptake in roots. Notes: Homeodomain proteins are transcription factors. Homeoproteins cooperate to control cell fate specification.

Construct: YP0122 Promoter candidate I.D: 11768849 cDNA LD: 13593439 (OCKHAM3-C) Lines expressing: YP0122-01, -02

Sequence (SEQ ID NO: 83):

agtttaatøttgtøtctat∞aatcaatttttttttctaaactg^ aagaaaataaagaatagaggtagacacctgtagtattttcttggtcagaccaataattat aattcaacgte^ aaaatacacaaattøaatgtataattgtgaggcatttatetgcgggaaaaaataaaata aaaagaatattø agatatatatagagagagagattaaaacattgatggctag<tatagagtctatggca gggtcatgatcacctatctt^ aattttattttaccattttataattctttatttttgcctgtagtacaatttacagaccca tactaaaagaaaaattaaattttgtcaaagtacaa cttttcttctrtctctctctgtøtatctcttctgttteatt aaactactttcttgiHgggttttaggagctatctcfattgtcttggttctgatacaaagM gtaattttcatggtatgagaaratttgcctttciBM atgggttcλgtagatcttaatgaaacttctgttmgcccaaaaagagttttcttttttct tctcttcttttttggttttcaattc

Promoter Expression Report # 108.02 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Flower M vascular Silique M placenta, M vascular Hypocotyl H vascular Cotyledon H vascular, H petiole Primary Root H vascular Observed expression pattern: Tl mature: GFP expressed in vasculature of silique and pedicles of flowers. T2 seedling: High GFP expression throughout vasculature of root, hypocotyl, and petioles- Expected expression pattern: Expressed in mid torpedo stage of embryos and root. Selection Criteria: Literature Bodenlos, (same as IAA 12 Hamann et al. (1999). Develop. Vol. 126: 1387- 1395. Gene: Indoleacetic acid-induced protein 12 GenBank: NM 100334 Arabidopsis thaliana auxin-responsive protein IAA12 (Indoleacetic acid-induced protein 12) (Atlg04550) mRNA, complete cds gi[30678909| ref]NM 100334.2 Source Promoter Organism: Arabidopsis thaliana, Ws ecotype Vector: pNewbin4-HAPl-GFP Marker Type: GFP-ER Generation Screened: XTl Mature XT2 Seedling DT2 Mature DT3 Seedling

Tl Mature Plant Expression Organs/Tissues screened Events Screened: n=5 Events Expressing: n=2 GFP Expression Detected X Flower Dpedicel G receptacle D nectary D sepal Dpetal D filament □ anther Dpollen Dcarpel Dstyle Dpapillae L vascular Depidermis Dstomata Dtrichome D silique X Silique D stigma Dstyle Dcarpel D septum M placenta D transmitting tissue M vascular Depidermis Dstomata D abscission zone D ovule D Ovule Pre-fertilization: Dinner integument D outer integument D embryo sac D funiculus Dchalaza Dmicropyle Dgametophyte Post-fertilization: Dzygote D embryo sack Dinner integument Douter integument D endothelium D seed coat Dprimordia Dchalaza Dmicropyle Dearly endosperm D mature endosperm D embryo D Embryo Dsuspensor Dpreglobular D globular D heart D torpedo D late D mature Dprovascular Dhypophysis Dradicle Dcotyledons Dhypocotyl D Stem Depidermis Dcortex Dvascular Dxylem Dphloem Dpith Dstomata Dtrichome D Leaf Dpetiole Dmesophyll Dvascular Depidermis Dtrichome Dprimordia Dstomata D stipule Dmargin D Shoot apical meristem D shoot apical meristem D flower primordium X in the Vasculature (Vs) of the flower and the Silique (Si)

Promoter utility Trait Area: Nutrients, PG&D Sub-trait Area: Plant size, root architecture, Utility: Modulation of nutrient uptake, loading and transport. Modulation of root growth and development.

Construct: YP0226 Promoter candidate LD: 13148249 cDNA LD: 12327003 Lines expressing: YP0226 -02, -05

Sequence (SEQ ID NO: 84):

ctccttgaiaatgattttgatcaaaagtgtaatttccacaaaccaattgcgcctgca aaa cttttcccaccattttttttccctcactttctttataatctt^ tatgcaaatggcaiBtgaccataaacgagtttgctagtataaagaatgatggccaacctg ttaaagagagactaaaattaggtctaaaatctaggagcaatgtaaccaatacatagtat atgaaatatøaaagttaaiftagattttttgatagcccaaattaaagaaaaatggtatt taaaara aaccacεiagcggaaaagaaaacaaaaaaaaciBtatiHtgatgcaacagcaacacaaa gcaacaccttgcacacacacatacaactg^aaacaagtttcttgggactctctattttct cttgctgcttgaaccaaacacaacaacgatatcccaacgagagcacaacaggtttgatta tgtcggaagacaagttttgagagaaaacaaacaatattttataacaaaggagaagac ttttggtaggaaaaattggtatgg∞attacaagacatatgggtcccaattctcatcac tø^ gtctctctcaaaccctaaatacartctttcrtcλcttgttgtctccattctctdgtgtc atcaagcttcttttttgtgtgggttaftt^ ttgttattgaca

Promoter Expression Report #110.02 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Flower H pollen Observed expression pattern: Tl mature: Pollen specific expression in mature plants. Expression appears to be prior to gametogenesis. T2 seedling: No GFP expression observed. Expected expression pattern: Gametophyte Selection Criteria: Arabidopsis knockout line, gametophytic lethal Gene: Ca2+-ATPase 7 GenBank: NM 127860 Arabidopsis thaliana potential calcium-transporting ATPase 7, plasma membrane-type (Ca2+-ATPase, isoform 7) (At2g22950) mRNA, complete cds gil 18400128|ref]NM 127860.11 [ 18400128] Source Promoter Organism: Arabidopsis thaliana, WS ecotype Vector: pNewbin4-HAPl-GFP Marker Type: GFP-ER Generation Screened: XTl Mature XT2 Seedling DT2 Mature DT3 Seedling Tl Mature Plant Expression Organs/Tissues screened Events Screened: n=6 Events Expressing: n=3 GFP Expression Detected X Flower Dpedicel Dreceptacle Dnectary Dsepal Dpetal Dfilament Danther H pollen D carpel D style Dpapillae D vascular D epidermis Dstomata Dtrichome Dsilique D Silique Dstigma Dstyle Gcarpel Dseptum Dplacentae Dtransmitting tissue Dvascular D epidermis Dstomata D abscission zone Q ovule D Ovule Pre-fertilization: Dinner integument Douter integument Dembryo sac D funiculus Dchalaza Dmicropyle Dgametophyte Post-fertilization: Dzygote D embryo sack Dinner integument Douter integument Dendothelium Dseed coat Dprimordia Dchalaza Dmicropyle Dearly endosperm D mature endosperm Dembryo D Embryo Dsuspensor Dpreglobular D globular D heart D torpedo D late D mature Dprovascular Dhypophysis Dradicle D cotyledons Dhypocotyl D Stem Oepidermis Dcortex Dvascular Dxylem Dphloem Dpith Dstomata Dtrichome D Leaf Dpetiole Dmesophyll Dvascular Depidermis Dtrichome Dprimordia Dstomata D stipule Dmargin D Shoot apical meristem | D shoot apical meristem D flower primordium X in the pollen

T2 Seedling Expression Tissues Screened Events Screened: n=3 Events Expressing: n=0 Seedlings expressing / Seedlings screened Event-01: 0/6 Event-02: 0/6 Event-03: 0/6 D Scheduled No GFP Expression Detected

Promoter utility Trait Area: Reproductive biology Sub-trait Area: Yield Utility: Modulation of pollen tube growth, gametophytic incompatibility. Notes: Modulation of breeding biology for example to generate male steriles.

Construct: YP0244 Promoter candidate I.D: 11768821 cDNA LD: 12736016 (OCKHAM3-C) Lines expressing: YP0244 -01, -02, -10

Sequence (SEQ ID NO: 85):

ttgatttgatgtatagttactatttaaagtcttatttgtgaaattttacaaatgttggaa aaaagcaMatggtgctatatttgtcagtttcccttgattatatatcctttt gtaatgflttttttatgtgtgtgtattcatgaaccttggaaaaactacaaatcagatcat ggtctgttttaggtgaaaaatttagaacacagttacgcaagaaagatatcggt aaatttttgtttctttgaatcgaaattaatcaaaaagtattttccattatataacaacaa ctaatctctgLLULlUULllltUlaacaactaatctcttatcaaaatgacactacag aatcacgattgtaaatcttcaaaagggcagtctgaaaaaatattcatgaggatgagattt tattcattcatggttgtaagtaatcattatgtaaagtttaggataaggacgt tcaaaatcatataaaaaaactctacgaataaagtttatagtctatcatattgattcatat ttcatagaaagttactggaaaacattacacaagtattctcgatttttacgagttt gtttagtagtcgcaaaartttattttacttttgagtatacgaacccataagctgattttc tttccaagttccaataatgatatcatagtgtactcttcatgaatgtttcaagcatat aattataacgttcataagtaacattctactgcatgtttgttattataaattaactaataa tcgaacgtatgagttttggttgagattgttgtgctcacgaaatgaaggactcgg tcaattctaaagcttaaaataagaagctcagatcttaaaactcgctttcgtcttcgtcct ccatttaagtttgcgattcttttgctcttctttctctctcacatttttgtcccaaaa caataaaaagaaacaataatagaaagtgttacagaaaaagaaagaaaactatcattgaag ttgggaaggagaaa

Promoter Expression Report #111 Promoter Tested In: Arabidopsis ihaliana, Wassilewskija (WS) ecotype Spatial expression summary: Flower L pedicel L epidermis Stem L epidermis Hypocotyl H epidermis Cotyledon H mesophyll H vascular H epidermis H petiole Rosette Leaf H epidermis H petiole Primary Root H epidermis Lateral root H lateral root cap Observed expression pattern: Tl mature: Low epidermal expression in stem and pedicles near inflorescence apical meristem. T2 seedling: High epidermal expression in cotyledons, petioles of emerging rosette leaves, hypocotyl, and root. Expression observed in vascular and mesophyll cells of cotyledons. Expected expression pattern: Drought inducible Selection Criteria: Ceres expression data Gene: Hypothetical protein GenBank: NM 102758 Arabidopsis ihaliana hypothetical protein (Atlg30190) mRNA, complete cds gi]18397396|ref]NM 102758.1|[18397396] Source Promoter Organism: Arabidopsis thaliana, WS ecotype Vector: pNewbin4-HAPl-GFP Marker Type: GFP-ER Generation Screened: XTl Mature XT2 Seedling DT2 Mature DT3 Seedling

Tl Mature Plant Expression Organs/Tissues screened Events Screened: n=6 Events Expressing: n=2 GFP Expression Detected X Flower L pedicel Dreceptacle Dnectary Dsepal Dpetal Dfilament Danther Gpollen Dcarpel Dstyle Dpapillae Dvascular L epidermis Dstomata Dtrichome Dsilique D Silique Dstigma Dstyle Dcarpel Dseptum Dplacentae Dtransmitting tissue Dvascular D epidermis Dstomata D abscission zone D ovule D Ovule Pre-fertilization: Dinner integument D outer integument Dembryo sac Dfuniculus Dchalaza Dmicropyle Dgametophyte Post-fertilization: D zygote D embryo sack Dinner integument D outer integument D endothelium D seed coat Dprimordia Dchalaza Gmicropyle Dearly endosperm Dmature endosperm Dembryo D Embryo Dsuspensor Dpreglobular Dglobular Dheart Dtorpedo Dlate Dmature Dprovascular Dhypophysis Dradicle Dcotyledons Dhypocotyl X Stem L epidermis Dcortex Dvascular Dxylem Dphloem Dpith Dstomata Dtrichome

Promoter utility Trait Area: Water use efficiency Sub-trait Area: Drought Utility: Among other uses this promoter sequence can be useful to improve: Modulation growth and development. Modulation of nutrient uptake and loading. Expression of nitrate transports and water pumps. Modulation of drought responses, including modulation of water uptake and transport under drought conditions. Notes: Endogenous promoter induced under drought.

Construct: YP0286 Promoter candidate LD: 11768589 cDNA LD: 12669548 (OCKHAM3-C) Lines expressing: YP0286 -04, -06;

Sequence (SEQ ID NO: 86):

gaaaacaatcataggttacgctattatcatcgaaaggMgtgatgcatattc∞attgaa ccag atgggtaactcacffigacgtgtagføcgtggaagaatagttagctøtcacgcataca t atøctgatttcatgcjatatatgtattatttatttatttttaataaagaagcgattggt gttttca catgtattaattttttccccttaaatcatttcagttgataata^ accgaggatatalHtgattgtgttgrtgtctattgatgatgtgtcgatattatgcttgtt gtaccaatgctcgagccgagcgtgatcgatgccttgacaaactatatatgttt aagttttgtatcttaattagaataacatttttatacaatgtaatttctcaagcagacaag atatgtatcctatattaattactataMgaatt^ gagcccattagtttccacgtaaatcacaatgacgcgacaaaatctagaatcgtgtc^ caacgccatgaatctctatataaaggctacaacaccacaaaggatcatcagtcatcacaa ∞acatta^

Tl Mature Plant Expression Organs/Tissues screened Events Screened: n=9 Events Expressing: n=3 GFP Expression Detected X Flower Dpedicel Dreceptacle Dnectary Dsepal Dpetal Dfilament L anther Dpollen Dcarpel Dstyle Gpapillae Dvascular Depidermis Dstomata Gtrichome Dsilique □ Silique Dstigma Dstyle Dcarpel Dseptum Gplacentae Dtransmitting tissue Dvascular Depidermis Dstomata D abscission zone D ovule X Ovule Pre-fertilization: Dinner integument Douter integument D embryo sac Dfuniculus Dchalaza Dmicropyle Dgametophyte Post-fertilization: Dzygote D embryo sack Dinner integument Douter integument L endothelium Dseed coat Dprimordia Dchalaza Dmicropyle Dearly endosperm Dmature endosperm Dembryo

T2 Seedling Expression Tissues Screened Events Screened: n=9 Events Expressing: n=3 Seedlings expressing / Seedlings screened Event-01: 5/6 Event-02: 3/6 Event-03: Not tested D Scheduled GFP Expression Detected D Hypocotyl Depidermis Dcortex IHvascular Dxylem Dphloem Dstomata X Cotyledon Dmesophyll Dvascular H epidermis Dmargin H petiole Dstomata Dhydathode X Rosette Leaf Dmesophyll Dvascular Depidermis H trichome Dpetiole Dprimordia Dstomata Dstipule Dmargin Dhydathode X Primary Root H epidermis Dtrichoblast Datrichoblast Dcortex Dendodermis Dvascular Dxylem Dphloem Dpericycle Dquiescent D columella D root cap H root hairs D Lateral root Depidermis Dtrichoblast Datrichoblast Dcortex Dendodermis D initials D flanking cells Dvascular D lateral root cap D Shoot apical meristem D shoot apical meristem X in the Epidermis (Ep) of the Cotyledon (Co), cotyledon petiole and root X in the Guard Cells (Gc) of the coteledon X in the Hypocotyl (Hy)-root transition zone X in the Root hair (Rh) X in the Trichomes (Tc) of the Rosette leaf (Rl)

Induction Screens 1. Drought - No differences observed. 2. Drought - No differences observed.

Promoter utility Trait Area: PG&D Sub-trait Area: Yield, Germination and mobilization of nutrient reserves Utility: Among other uses this promoter sequence can be useful to improve: Modulation seed size and seed shape. Modulation of pollen development and dehiscence and engineering of male sterility. Engineering of plant responses to insects and production and loading of volatiles into trichomes and other epidermal cells. Alteration in loading and transport of metabolites from the soil and environment. Protection against insects and microbes. Modulation of root signaling. Drive CDS that can play a role in shade avoidance. Notes: Endogenous promoter is up-regulated in far red light.

Construct: YP0289 Promoter candidate I.D: 11768596 cDNA LD: 12326995 Lines expressing: YP0289 -03; 6/4/03 , -10, -13

Seqeunce(SEQ ID NO: 87):

atggacttttctti^ttatatggtcaaacaattactgctcaatgtatttgcgtatag agcatgtcxaataccatgcctcatgatgtgagattgcgaggcggagtcagagaa^ gtgacgacgllLtlLlglllltlLlgggcatagtgtaaagtgatattaaaatttcatggt tggcaggtgartgaaaataaaaatgtgtataggatgtgtttatatgclHgacggaaaaat agte ctcaactaatacagatctttataaagagtatataagtcMggttaatcatgaatggcaata iaiHagagtagatgagatttatgtttatBttgaa^ caatggcaaaatatetagtcaaatcaaacrtggffictgataatatatgtgttgaatca^ ^ ataactacattttaataciagtagctattagctgtatttcaiaatcaatttc^^ aactBctgcattcagagaataaagctatdtactaaacacrtacttttaaagfttcffiff i^ cttacaciaattttattttctttgtttgaiaacaaatttatcggctaagcatcaxλaaa tttaatacacgttatgaagactaaa^ ccttccctac∞artcacatrtct∞acgtggcaatctttgatattgacaccto ctttataaacccca

Promoter Expression Report # 116 I "V Ig 'JIlI Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Flower L pedicel, L sepal, H carpel, H epidermis, L stomata, H silique Silique H carpel, H epidermis, L stomata, L abscission zone Leaf H mesophyll, L vascular, H epidermis Observed expression pattern: Tl mature: GFP expression at the base of sepals at abscission zone of developing and mature flowers. High expression specific to carpels of developing and mature siliques. T2 seedling: Weak root hair expression at hypocotyl transition zone observed in 1 in 6 seedlings and in onlyl of 2 events screened. Expected expression pattern: Flowers, seed, roots. Selection Criteria: Arabidopsis public; containing AP2 DNA binding domain. Gene: EREBP-2 GenBank: NM 124093 Arabidopsis thaliana ethylene responsive element binding factor 2 (EREBP-2) (At5g47220) mRNA, complete cds gi|30695135]ref1NM 124093.2p0695135] Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewbin4-H AP 1 -GFP Marker Type: GFP-ER Generation Screened: X Tl Mature XT2 Seedling DT2 Mature D T3 Seedling

Tl Mature Plant Expression Organs/Tissues screened Events Screened: n=4 Events Expressing: n=3 GFP Expression Detected X Flower L pedicel Dreceptacle Dnectary L sepal Dpetal Dfilament Danther Dpollen H carpel Dstyle Dpapillae Dvascular H epidermis L stomata Dtrichome H silique X Silique Dstigma Gstyle H carpel Dseptum Dplacentae Dtransmitting tissue Dvascular H epidermis L stomata L abscission zone D ovule D Ovule Pre-fertilization: Dinner integument D outer integument D embryo sack

Promoter utility Utility: Increased leaf size and volume, increased source capacity. Tolerance to drought. Improved performance in low nitrogen conditions. Larger siliques, increased seed number. Increased seed yield. Altered dehiscence and seed scatter.

Construct: YPOO 15 Promoter candidate LD: 11768611 cDNA LD: 13612380 Lines expressing: YP0015 -03, -04

Sequence (SEQ ID NO: 88): ttgagccttattgttgttattgacttttagccaategaaagagatggaaattcaataalt atcca^ aagaaaagaaatgcaacaaatetggattaaartgtggtttg^aaattgagctttgcatga aaartttatcactatgatttcactHctccatatttatt^

ttggtgaccaggagaaatgtatcccgatttttgcagacactttcagtgtccccattc atataattatggcccacctcgttaagatttttcattcacca∞ rfltgtafltfaaaoatataatafa^gCfflatflCfcfofaata^ agtataatgaattaagaccttggttttcgattaacaaactaattaaacactagttttgcc taataaaaccgggaatcgtattcaaaaccgaa^ caaaaccaaatcagcatctttcttccagaaatgtcatgaccacatgacgtcatctt^^ gaaaaatccacaactttcatattttttaattacccacteaactaaaacaaatttggaaaa acatgaaaaactMctt^

Promoter Expression Report # 118 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Flower H anther H pollen L vascular H stomata Silique M ovule Ovule Pre-fertilization: H outer integument H embryo sack H gametophyte Post-fertilization: M outer integument H seed coat H embryo Embryo H suspensor H heart H late H mature L radicle L cotyledons Stem H epidermis H stomata H trichome Leaf H stomata Hypocotyl H epidermis L cortex H stomata Cotyledon H mesophyll H vascular H epidermis H stomata Rosette Leaf H stomata Primary Root H cortex Observed expression pattern: Tl mature: Expressed in pollen cells throughout development. Expression visible during pollination when dehisced pollen attaches to stigma resulting in extension of the cell wall to establish an attachment site or "foot". Once attached, pollen is hydrated and germination of pollen tubes follows through the stigma at the attachment site. These processes are likely targets in regulation of self-incompatibility and species-specific pollen recognition. No expression is observed after hydration. Expressed in egg sack of pre-fertilized ovules, inner integument, endosperm, heart stage embryo and suspensor cells of developing ovules. High specific expression in and epidermal cell files flanking trichomes of stem and guard cells throughout mature plant. T2 seedling: High expression in epidermal and guard cells throughout seedling. High expression epidermal, vascular, and mesophyll cells of cotyledons. Not observed in leaf primordia. High expression specific to cortical cells of root. Expected expression pattern: Induced prior to cell division and usually associated with dividing cells. Selection Criteria: Ceres BLAST search homology; CDC2-like protein Gene: putative protein kinase /note="similar to cyclin-dependent kinase cdc2MsE [Medicago sativa]

GenBank: NM 125756 Arabidopsis thaliana protein kinase, putative (At5g63610) mRNA, complete cds gi|306978711ref1NM 125756.2|[306978711 Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewbin4-H AP 1 -GFP Marker Type: GFP-ER Generation Screened: XTl Mature XT2 Seedling DT2 Mature DT3 Seedling

Tl Mature Plant Expression Organs/Tissues screened Events Screened: n=5 Events Expressing: n= 2 D No GFP Expression Detected X Flower Dpedicel Dreceptacle Dnectary Dsepal Dpetal Dfilament H anther H pollen D carpel D style Dpapillae L vascular D epidermis H stomata Dtrichome D silique

T2 Seedling Expression Tissues Screened Events Screened: n= 2 Events Expressing: n= 2 Seedlings expressing / Seedlings screened Event-01: 5/5 Event-02: 1/5 D Scheduled GFP Expression Detected X Hypocotyl H epidermis L cortex Dvascular Dxylem Dphloem H stomata X Cotyledon H mesophyll H vascular H epidermis Dmargin H stomata Dhydathode D Rosette Leaf Dmesophyll Dvascular Depidermis Dtrichome Dpetiole Dprimordia H stomata D stipule Dmargin Dhydathode X Primary Root Depidermis Dtrichoblast Datrichoblast H cortex Dendodermis Dvascular Dxylem Dphloem Dpericycle D quiescent Dcolumella Droot cap Droot hairs D Lateral root Depidermis Dtrichoblast Datrichoblast Dcortex Dendodermis Dinitials Dflanking cells Dvascular Dlateral root cap D Shoot apical meristem D Shoot apical meristem X in the Epidermis (Ep) and Guard cells (Gc) of the hypocotyl apex X in the Guard cells (Gc), Vascular (Vs) and Mesophyll (Me) of the cotyledon X in the Epidermis (Ep) and Cortex (Cr) of the root transition zone X in the Cortex (Cr) and Nucleus (Nu) of the root Promoter utility Trait Area: SSR Sub-trait Area: Sink strength, embryo size Utility: Imprint modulation through male, larger (heavier) seeds, smaller (lighter) seeds. Male sterility and altered breeding barriers. Altered pollen composition. Altered fertility. Resistance to drought.

Construct: YP0230 Promoter candidate I.D : 13148201 cDNA LD: 12676237 Lines expressing: YP0230 -02, -03 (9/08/03)

Sequence (SEQ ID NO: 89):

aatcattaaatctttgatgagaaatatccaatctactaatgtatatcgatgatttaa atgaaattacttatttgaacacaaaaataaatgaatttactaataaataaatagcgtagt tgcgagca agtggctaaaaaaattacaaatctagtttccattctcagcggcggggtgcft^ ttttgtctactctcctccatctccgaggttctccaagctcagctcctcttcccatcattc atccgaccgcctotccggtcagatcctttac atgtaaaaacagatcgtataaggcctcgaagtttttaatttgaaagtggtatcgaaaitt tttggtctttgattaggttagggcaccgtagctctg ttattggtctttggagcttggtaatttctgdgaattgattgatαx^^ cgtcaattgctgataatttggagtctttgtgtctgaatttgttcatatgaagttttctga gggatgtgaattttattgtctgctaattttgaaacg^ gatdttttrtgttaagtttcttgcttgtaagttMggatcacttga%agtø aactacaaagaggaaaccaagatggttttgttttgttagadctaccccttttccgattca catggtaaggacattgaggtag

Promoter Expression Report # 119 "1K- Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Silique H ovule Ovule Pre-fertilization: H outer integument Post-fertilization: H outer integument, H seed coat Observed expression pattern: Tl Mature: GFP expressed in outer integument early in ovule development through seed coat of mature seeds. T2 Seedling: No expression observed. Expected expression pattern: Expressed in ovules and different parts of seeds Selection Criteria: Greater than 5Ox up in pi ovule microarray Gene: "hypothetical protein /product="expressed protein" GenBank: NM 117365 Arabidopsis thaliana expressed protein (At4gl2960) mRNA, complete cds gi|30682287|reflNM 117365.2][30682287] Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewbin4-HAP 1 -GFP Marker Type: GFP-ER Generation Screened: XTl Mature XT2 Seedling DT2 Mature DT3 Seedling

Tl Mature Plant Expression Organs/Tissues screened Events Screened: n= 2 Events Expressing: n= 2 GFP Expression Detected D Flower Dpedicel Dreceptacle Dnectary Dsepal Dpetal Dfilament Danther Dpollen Dcarpel Dstyle Dpapillae Dvascular Depidermis Dstomata Dtrichome

Promoter utility Trait Area: Nutrition / Composition, SSR Sub-trait Area: Nitrogen use efficiency, seed size Utility: Seed size determination. Increased seed size. Altered seed composition. Tolerance of seeds to desiccation. Resistance of seeds to abortion. Increase sink strength by expression of A.A. transporters. Construct: YP0120 Promoter candidate LD: 11768656 cDNA LD: 12370095 Lines expressing: YP0120-01, -02

Sequence (SEQ ID NO: 90):

tegtttttgatttaatctecgtttttcttaatcataaatgggtaattatø^ gtgggagaactaatcgtttgtcctttctaaatctcacatattagaatttagaategtgtg ctacataagaatattagttcagctcggaacaactatt^ aatgcattattttatcaacatgcattttgaattgaatataaaatttcaiBattgtaaaga cataaattacataaaattttacatgaaaaaatagatatagaaagaaaatgaa atgctctcteaattttttaatdcataacaagaattcaaattaattagttcatatttttgg ttaatataacatttacct aaacggaaagttgaatttattcaaacttaaattcaatagcattaattaaaggcgaaagct ottatctctacatgtggttcaaactagacatccaatt^ ccaaaactactafagtttggcaatttgaaagatgcatcagaaciactcagacaggtaaaa gtagaac^^ aottaagatatatattapasfatata^gfffltggtsfflfrw^R^gattagM gqriaR^gfCgaatλλflλgλgacctcaCgtgaqRqtgttaP-gagrtagfaa

Tl Mature Plant Expression Organs/Tissues screened Events Screened: n=2 Events Expressing: n= 2 GFP Expression Detected X Flower Dpedicel Dreceptacle D nectary H sepal Dpetal Dfilament Danther Gpollen D carpel D style Dpapillae D vascular D epidermis D stomata Dtrichome Dsilique D Silique Dstigma Dstyle Dcarpel Dseptum Dplacentae Dtransmitting tissue Dvascular D epidermis D stomata D abscission zone D ovule D Ovule Pre-fertilization: Dinner integument G outer integument D embryo sack D funiculus Dchalaza Dmicropyle Dgametophyte Post-fertilization: D zygote D embryo sack Dinner integument D outer integument D endothelium D seed coat Dprimordia Dchalaza Dmicropyle

Promoter utility Trait Area: PG&D, SSR Sub-trait Area: Organ size, cell number, shape, source strength Utility: Leaf size and photosynthetic capacity. Increased source strength. Increased sucrose loading. Increased leaf expansion, resulting in improved seedling stress tolerance. Modulate size of organs, young leaf specific exp. Notes: Expansions are thought to hydrolyte non-covalent bonds with cell wall structural polysaccharides, allowing turgor-driven slippage and increase in cell volume.

Construct: YP0261 Promoter candidate LD: 11768750 cDNA LD: 12385291 Lines expressing: YP0261 -01, -03

Sequence (SEQ ID NO: 91):

ttffiaaattfiffifttatcgtRgffflgtfirefla^trøsgfgattπaa^ tcgttcttttgtatøttcatggcaagctctøttøtgactaagtcacal^ ffigaaacteattagaaatatatatatatatatatetattcaaitttagtaaattgfta^ cacaacacattactataataagttaaacttctttttttataggcgcctggaaaaaaaaga aaagcaacaagagggstgtgaggacgcatcaccng gtctctttgctttttcggtttttttcttgccaatcaatttattttgttcctcagaaaaaa gaaaatctaaaaccaaaatatatattataa^ aaaagtttttaittatcttgaccttatttctttgaagaaaataaagcttggttattaaag aagtccaagttagttgccaccatcagtggcataa^ agttttctataaattcaaccartcacctcccactctaaaacccaacaacataatt^ trtccatcrtacattcaattgtttctccrttaatttdctagtacatatttacttgtgciB iaagtaattgattttatatoacccatg^^

Promoter Expression Report # 121 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Flower H pedicel H receptacle H filament H anther H carpel H vascular H silique Silique H vascular Stem H vascular Leaf H vascular Hypocotyl L epidermis H vascular Cotyledon H vascular L epidermis Rosette Leaf H epidermis Primary Root H cortex H root cap Observed expression pattern: Tl Mature: High GFP expression in vasculature of stem and leaves, also pedicles, siliques and stamen of flowers. Not detected in sepals and petals. Expression in silique specific to medial vasculature. T2 Seedling: High GFP expression in hypocotyl and cotyledon vasculature and cortex of root. Not observed in root vasculature. Expected expression pattern: Stem and root elongation zones. Selection Criteria: Hanzawa Y, Takahashi T, Michael AJ. Burtin D, Long D, Pineiro M, Coupland G, Komeda Y. ACAULIS5, an Arabidopsis gene required for stem elongation, encodes a spermine synthase. EMBO J. 2000 Aug 15;19(16):4248-56. Gene: Arabidopsis ACAULIS5 (ACL5) GenBank: NM 121958 Arabidopsis thaliana spermine synthase (ACL5) (At5gl9530) mRNA, complete cds gij306873631ref]NM 121958.2P0687363] Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewbin4-HAP 1 -GFP Marker Type: GFP-ER Generation Screened: XTl Mature XT2 Seedling DT2 Mature DT3 Seedling

Tl Mature Plant Expression Organs/Tissues screened Events Screened: n= 3 Events Expressing: n= 1 GFP Expression Detected X Flower H pedicel H receptacle Dnectary Dsepal Dpetal H filament H anther Dpollen H carpel D style Dpapillae H vascular D epidermis Dstomata □trichome H silique X Silique Dstigma Dstyle Dcarpel Dseptum Dplacentae D transmitting tissue H vascular D epidermis Dstomata D abscission zone D ovule D Ovule Pre-fertilization: Dinner integument D outer integument D embryo sack Dfuniculus Dchalaza Dmicropyle Dgametophyte Post-fertilization: D zygote D embryo sack Dinner integument D outer integument D endothelium D seed coat Dprimordia Dchalaza Dmicropyle Dearly endosperm D mature endosperm D embryo D Embryo Dsuspensor Dpreglobular Dglobular Dheart Dtorpedo Dlate Dmature " Dprovascular Ohypophysis Dradicle Dcotyledons Dhypocotyl

Promoter utility Trait Area: Nutrition Sub-trait Area: Low nitrogen tolerance Utility: Translocation to the flower, seed fill. Sucrose loading and transport. Improved source capacity, lending to larger plant organs, larger plants, Increased biomass, increased yield. Improved root growth and soil penetration. Resistance to drought, improved uptake of nitrogen and phosphate. Modify nitrate uptake and translocation to Xylem Notes: Spermine is an alkaloid. Construct: YP0263 Promoter candidate LD: 11768752 cDNA LD: 12640578 Lines expressing: YP0263 -10, -11, -12

Sequence (SEQ ID NO: 92): atctegdgtggatt∞accaaaattctggcagggαiatgatctaaaaactgagactgc gcgtg^ gtaaccaaacataaggagaacctaattacattattgttttaatttcgtcaaactggtttt e^ qgaRagatcatagtggattaatatgttgagaggtcagaa^tt^ggtføa^ ttaaaagtgatgttatggtttgaggggaaaaaggflgatgtcaactaagaiagttgaagt aatgtcttaaactaaagtaaaccaccggtccaaccgtggt gatttatcctaaaaatcaaaaiHgtagaaacatacttlHaaiHtatacattgatcggacg aaaattgtaaactagtatagttteaaaaactagttga^ caaacttaaacactaaagaacatatatgaatagaagtttaiateaattactatatatcta ccataaatctcttataattatgatgtcacgatgaggaagt ataagcatgcgacggaattttggcagaagattgtagagttgtaatctgtcgcaatcatta ctcgtgctagcatttttcattttcccλcatttgtggatø ccaacaagattctataaaaacgcaaaggttgtctccatagaatatcgtc

Promoter Expression Report # 123 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Flower M stomata Silique M stomata Ovule Post-fertilization: L embryo Embryo L provascular, L cotyledons Primary Root L epidermis, L xylem Observed expression pattern: Tl mature: Strong expression in embryonic vascular tissue of cotyledons. Weak guard cell expression in flower pedicle and silique. T2 seedling: Weak degrading root epidermis expression near transition zone. Weak root vascular expression in elongation zone. Expression in very thin cell layer appears to be xylem. Expected expression pattern: root, flowers, ovules, young silique Selection Criteria: Arabidopsis Two component line CS9135. Gene: Hypothetical protein containing helix-loop-helix DNA binding domain. GenBank: NM 116493 Arabidopsis thaliana bHLH protein (At4g02590) mRNA, complete cds gi|30679204|ref]NM. 116493.2| [30679204] Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewbin4-HAP 1 -GFP Marker Type: GFP-ER Generation Screened: XTl Mature XT2 Seedling D T2 Mature D T3 Seedling

Tl Mature Plant Expression Organs/Tissues screened Events Screened: n=3 Events Expressing: n=2 GFP Expression Detected X Flower Dpedicel Dreceptacle Dnectary G sepal Dpetal D filament D anther Dpollen Dcarpel Dstyle Dpapillae Dvascular Depidermis M stomata Dtrichome D silique X Silique D stigma Dstyle Dcarpel D septum Dplacentae D transmitting tissue Dvascular Depidermis M stomata D abscission zone D ovule X Ovule Pre-fertilization: Dinner integument Douter integument Dembryo sack Ofimiculus Dchalaza Dmicropyle Dgametophyte Post-fertilization: Dzygote D embryo sack Dinner integument Douter

Promoter utility Trait Area: SSR Sub-trait Area: Sink strength enhancement and size. Utility: Translocation to cotyledons, seed fill. Increased embryo and seed weight. Altered embryo and seed composition. Improved seedling vigor, seedling resistance to drought, cold, cold/wet conditions.

Construct: YP0003 Promoter candidate LD: 13148213 cDNA I.D: 12649228 Lines expressing: YP0003 -04, -06

Sequence (SEQ ID NO: 93): tggatctgctagatatatgagaacgaaagaaccagaagctattagaggcgggaggagata tgtggggatgatttcagtgcaattccacgacgcacca tttccactttcgtaacacctaaacgaccgcttcggccgtataaaatcgcaaatgtttggt ctcagtgtatttttccaatttccaaatacatcaattcaaattatat aatatctagtggcaattataagtatatcatatattttcaaaattaattaaaaagattact aaattatgtttgactacaactattataatagttaaaaacataaacaa aaacaaagaaactattttaataaaaaaatcaagtaaacattaaaacataagcaaaaaata atgttaaagaaattattaattattaatttactaataattaatac ctctataaattaattgttagaggtttaacgtaatttataaggaaaactaaagaagacttt aacccataaagaaaaaaacaaagactgaattgaaggcccat atttagaagaagagaaagaagacccaaatatgatataaaatccagcccatttatatattt ttattttgtttctggaaggaaaataagaaaatggcaaaaacg aaataatctgaaaaagtaaggtctttøccaaaaaggatatfittfttataaacagagca taa^ tcctcattcgttttaaagcatcaaaatttcatcaacccaaaatagattaaaaaaatctgt agctttcgcatgtaaatctctctttgaaggttcctaactcgttaat cgtaactcacagtgactcgttcgagtcaaagt

Promoter Expression Report #125 Promoter Tested In: Arabidopsis thaliana, Wassilewskija (WS) ecotype Spatial expression summary: Flower H pedicel H petal H epidermis Silique H stigma L style L carpel L septum L epidermis Ovule Post-fertilization: H outer integument Stem H epidermis H stomata Hypocotyl H epidermis Cotyledon H epidermis Rosette Leaf H epidermis H trichome Observed expression pattern: Tl mature: GFP expression specific to epidermal cell types. High GFP expression in epidermis of stem decreasing toward pedicles and inflorescence apex. In the flower, high expression observed in epidermal cells of petals and stigma, and lower expression in carpels. High expression in outer integuments of maturing ovules. High expression throughout epidermal cells of mature lower stem. T2 seedling: GFP expression specific to epidermal cell types. High expression in epidermis of hypocotyl, cotyledon, and trichomes of rosette leaves. Not detected in root. Expected expression pattern: Drought inducible Selection Criteria: Public reference; MoI Gen Genet. 1993 Apr; 238 (1-2): 17-25. Gene: Dehydration-induced protein RD22 GenBank: NM 122472 Arabidopsis thaliana dehydration-induced protein RD22 (At5g25610) mRNA, complete cds gi|306899601ref|NM 122472.2][306899601 Source Promoter Organism: Arabidopsis thaliana, WS ecotype Vector: pNewbin4-HAPl-GFP Marker Type: GFP-ER Generation Screened: XTl Mature XT2 Seedling DT2 Mature D T3 Seedling

Tl Mature Plant Expression Organs/Tissues screened Events Screened: n= 2 Events Expressing: n= 2 GFP Expression Detected X Flower H pedicel Dreceptacle Dnectary Dsepal H petal Dfϊlament Danther Dpollen □carpel Dstyle Dpapillae Dvascular H epidermis Dstomata Dtrichome □ silique X Silique H stigma L style L carpel L septum Dplacentae D transmitting tissue □vascular L epidermis Dstomata Dabscission zone Dovule X Ovule Pre-fertilization: Dinner integument □ outer integument □ embryo sac □funiculus Dchalaza Dmicropyle Dgametophyte Post-fertilization: Dzygote □ embryo sack Dinner integument H outer integument □ endothelium Dseed coat Dprimordia Dchalaza Dmicropyle □ early endosperm Dmature endosperm □ embryo D Embryo □suspensor Dpreglobular □ globular Dheart Dtorpedo D late Dmature □provascular Dhypophysis Dradicle Dcotyledons Dhypocotyl X Stem H epidermis Dcortex Dvascular Dxylem Dphloem Dpith H stomata Dtrichome

D Leaf □petiole Dmesophyll Dvascular Depidermis Dtrichome Dprimordia Dstomata D stipule □ margin D Shoot apical meristeni D shoot apical meristem □ flower primordium X in the Pedicel (Pd) of the inflorescence meristem and the flower X in the item below the inflorescence meristem X in the Stigma (Sg) of the flower X in the Petal (Pe) of the flower X in the Style (Sy) and Carpel (Ca) of the silique X in the Outer integument (Oi) of the ovule X in the Epidermis (Ep) of the stem

Promoter utility Trait Area: PG&D, Stress, Nutrients Sub-trait Area: Drought, heat and cold Utility: Among other uses this promoter sequence can be useful to improve: Modulation of compatibility/incompatibility. Modulation seed size and seed shape. Modulation of stem size and shape. Protection against insects and microbes. Modulation of ROS signaling. Uptake of nutrients. Modulation of drought responses including leaf and flower wilting, ovule abortion, infertility and seed abortion. Alteration of seed development and shape, attention of seed dormancy and germination. Notes: Goh CH, Nam HG, Park YS. Stress memory in plants: a negative regulation of stomatal response and transient induction of rd22 gene to light in abscisic acid-entrained Arabidopsis plants. Plant J. 2003 Oct;36 (2):240-55. Iwasaki T, Yamaguchi-Shinozaki K. Shinozaki K. Identification of a cis-regulatory region of a gene in Arabidopsis thaliana whose induction by dehydration is mediated by abscisic acid and requires protein synthesis. MoI Gen Genet. 1995 May 20;247 (4):391-8. Yamaguchi-Shinozaki K, Shinozaki K. The plant hormone abscisic acid mediates the drought-induced expression but not the seed-specific expression of rd22, a gene responsive to dehydration stress in Arabidopsis thaliana. MoI Gen Genet. 1993 Apr;238 (l-2):17-25. Endogenous promoter is down-regulated in roots and only mildly and inconsistently up-regulated in drought.

Construct: YP0356 Promoter candidate I.D: 11768602 cDNA LD: 12394809 Lines expressing: YP0356 -01, -04

Sequence (SEQ ID NO: 94):

tøgttcattgaaacgtcaacMttacrtgcaaccactttgtaggacxattaactgc ggtttactggttcfelaaatc^taaatccaaatatgaagtatggcaataataataacatg ttagcacaaaaaaiBctcattaaattccλac∞ atataracaaiaatagtgatacaaagtaggtcttgatattcaactattcgggatWctgg^ c&agltttaactagtagaaattgaaatttttaaaaattgtacttac^iaaaaffig aatcaatate aataatfcgaaaatatctgaactaagtøgtagttttaaaatataatcccggtttggacc ^ ccagattgatctattacaaatttcacctgtcaacgctaactccgaacttaatcaaagatt ttgagctaaggaaaacteatcagtgatcacccaaagaaaacattcgtgaataa ttcxatggcagcaaaacaaalHggacccaaalBggaatgtcaaaaaaaagaaagacacga aacgaagtagtataacgtaacacacaaaaataaactagagatattaaaaacacat gtccacacatggaiacaagagcatttaaggagcagaaggcacgtagtggtiagaaggtat gtgaiHtaattaatcggcccaaatagattggtaagtagtagccgtctatatcatccat actcatcataacttcaacct

Promoter Expression Report #126 Promoter Tested In: Arabidopsis thaliana, Wassilewskija (WS) ecotype Spatial expression summary: Ovule Pre-fertilization: H outer integument Post-fertilization: L outer integument L chalaza Hypocotyl L vascular Primary Root L epidermis M vascular M pericycle Observed expression pattern: Tl mature: GFP expressed in outer integument of developing ovule primordium. Higher integument expression at chalazal pole observed through maturity. T2 seedling: Medium to low expression in root vascular bundles weakening toward hypocotyl. Weak expression in epidermal cells at root transition zone. Expected expression pattern: Drought inducible Selection Criteria: Ceres, Inc. Expression data Gene: Putative cytochrome P450 GenBank: NM_112814 Arabidopsis thaliana cytochrome P450, putative (At3g 19270) mRNA, complete cds gi|18402178]ref]NMJ.12814.1|[18402178] Source Promoter Organism: Arabidopsis thaliana, Columbia ecotype Vector: pNewbin4-HAPl-GFP Marker Type: GFP-ER Generation Screened: XTl Mature XT2 Seedling DT2 Mature DT3 Seedling

Tl Mature Plant Expression Organs/Tissues screened Events Screened: n=2 Events Expressing: n=2 GFP Expression Detected D Flower Dpedicel Dreceptacle Dnectary Dsepal Dpetal Dfϊlament Danther Dpollen Dcarpel Dstyle Dpapillae Dvascular Depidermis Dstomata Dtrichome Dsilique D Silique □stigma Dstyle □ carpel D septum Dplacentae □ transmitting tissue □ vascular □epidermis Dstomata □ abscission zone □ ovule X Ovule Pre-fertilization: Dinner integument H outer integument D embryo sac □funiculus Dchalaza Dmicropyle Dgametophyte Post-fertilization: D zygote □ embryo sack Dinner integument L outer integument Dendothelium Dseed coat Dprimordia L chalaza Dmicropyle □early endosperm D mature endosperm D embryo D Embryo Dsuspensor Dpreglobular □ globular D heart D torpedo D late □ mature Dprovascular Dhypophysis Dradicle Dcotyledons Ohypocotyl G Stem □epidermis Dcortex Dvascular Dxylem Dphloem Dpith Dstomata Dtrichome D Leaf Dpetiole Dmesophyll Dvascular Depidermis Dtrichome Dprimordia Dstomata D stipule D margin D Shoot apical meristem D shoot apical meristem D flower primordium

X in the Chalaza (Ch), Funiculus (Fn) and Outer integument (Oi) of the ovule primordial X in the Chalaza (Ch) in the pre-fertilized ovule X in the Chalaza (Ch) and Micropyle (Mp) of the fertilized ovule and developing seed X in the Chalaza (Ch) of the mature seed

Promoter utility Trait Area: Seed Biology, Stress Sub-trait Area: Seed fill, seed size, stress during seed fill, stress protection, endosperm production Utility: Among other uses this promoter sequence can be useful to improve: Protection against ovule and seed abortion. Modulation seed size, seed shape. Modulation of endosperm growth and development. Modulation of heat responses and protection against heat stress. Modulation of water and mineral ion uptake and transport. Loading and transport of metabolites into seeds. Modulation of breeding system. Notes: Heat stress is a major determinant of seed and fruit yield in many crops, and protection against transient heat stress is a primary goal of modern agriculture. This promoter 11768817 induces in leaves at approximately 24 hour after heat stress. The leaf induction can protect against water loss from the leaf, offering a possibility of protection against heat and drought conditions, which often coincide. Promoter is mildly induced by drought and drought-like conditions.

Construct: YP0374 Promoter candidate LD: 11768817 cDNA LD: 12370888 Lines expressing: YP0374-01, -02

Sequence (SEQ ID NO: 95): aagaca∞cgtaaatgttgtcatgtagaagaaactagaaacgttaaacgcatc^ acaagacgcgtatttctttcgaattctccaaaccattaccaMgatatataataccgacat gccgttgataaagtttgtatgcaaatcgttcattgggtatgagcaaatgccat^ tcttg^ttaaatggtα;aaaaatagtttgttcx;cactactagttactaatttgtatca ctctgcaaaataatcatgaiHtaaacgtatgtg acaatgcagagatgaccataaaagaacattaaaacactacttccactaaatctatggggt gccttggcaaggcaattgaafaaggagaatgcatcaagatgatatagaa^ cagtttataacattaatgt11tggcgga.aa.a.ttttctatatatt3gfic<ffic ffi tglagtttacactgctcattaccatgaaaaccaaggcagtaiBtaccaacattaataaac taaatcgcgatttctagcacccccattaattaa atλataλλC^firtλtatcatWa^taataaataagaaagaaatr^ tCλt(^CCraCgffiggargftaataλaaca.taar.Rflfafifλarl^

bottom half of secondary inflorescence of mature plants. No differences observed between primary and secondary inflorescence meritems.

Tl Mature Plant Expression Organs/Tissues screened Events Screened: n=2 Events Expressing: n=2 GFP Expression Detected X Flower Dpedicel Dreceptacle Dnectary M sepal M petal Dfilament Danther Dpollen D carpel D style Dpapillae D vascular M epidermis Dstomata Dtrichome Dsilique D Silique Dstigma Dstyle Dcarpel Dseptum Dplacentae D transmitting tissue Dvascular D epidermis Dstomata D abscission zone D funiculus □ ovule D Ovule Pre-fertilization: Dinner integument Douter integument Dembryo sac D funiculus Dchalaza Dmicropyle Dgametophyte Post-fertilization: Dzygote D embryo sack Dinner integument Douter integument D funiculus D endothelium D seed coat Dprimordia Dchalaza Dmicropyle Dearly endosperm D mature endosperm Dembryo D Embryo Dsuspensor Dpreglobular Dglobular Dheart Dtorpedo Dlate Dmature Dprovascular Dhypophysis Dradicle Dcotyledons Dhypocotyl D Stem Depidermis Dcortex Dvascular Dxylem Dphloem Dpith Dstomata Dtrichome D Leaf Dpetiole Dmesophyll Dvascular Depidermis Dtrichome Dprimordia Dstomata D stipule D margin D Shoot apical ineristeni D shoot apical meristem D flower primordium X in the Petal (Pe), and Sepal (Se) of the flower X in the flower bud

Promoter utility Trait Area: Nutrients, Stress Sub-trait Area: Drought, Nitrogen uptake Utility: Among other uses this promoter sequence can be useful to improve: Modulation of water and nutrient uptake and seedling establishment. Modulation of plant-microbe interactions. Changes to flower development and structure. Modulation of responses to drought, especially in the flower and inflorescence and in the stigma. Changes in pollination biology and incompatibility upon drought. Notes: Endogenous promoter is up-regulated under PEG treatment but not with ABA and drought.

Construct: YP0377 Promoter candidate LD: 11768593 cDNA LD: 13613778 Lines expressing: YP0377 -02, -06

Sequence (SEQ ID NO: 96):

TATAAACCATTCCTATi^CACCαTATTTAAACαTA AATTTTAATTTGATTGAATTATACCCCCTTAATTGGATAΆλTTCΆAATATGTCAΆC TTTTTTTTTGTAAGATTTTTTTAT GGAAAAAAAAATTGATTATTCACTAAAAAGATGACAGGTTACTTATAATTTAATATATGT AAACCCTAAAAAGAAGAAAA TAGTTTCTGTTTTCACTTTAGGTCTTATTATCTAAACTTCTTTAAGAAAATCGCAATAAA TTGGTTTGAGTTCTAACTTT AAACΆCΆTTAATATTTGTGTGCTATTTAAAAAATAATTTACAAAAAAAAAAACAAATT GACAGAAAATATCAGGTTTTGT AATAAGATATTTCCTGATAAATATTTAGGGAATATAAαVTATCAAAAGATTCAAATTCT GAAAATCAAGAATGGTAGACA

GGCCAAACCTTTTTATAATCATGCTATTTATTTCCTTCATTTTTATTCTATTTGCTA TCTAACTGATTTTTCATTAACAT GATACCΆGAAATGIAATTTAGΆTGGATTAATTCTTTTCCATCCACGACATCTGGAAAC ACTTATCTCCTAATTAACCTTAC TTTTTTTTTAGTTTGTGTGCTCCTTCATAAAATCTATATTGTTTAAAACAAAGGTCAATA AATATAAATATGGATAAGTA TAATAAATCTTTATTGGATATTTCTTTTTTTAAAAAAGaAATAAATCTTTTTTGGATATT TTCGTGGCAGCATCATAATG AGAGACTACGTCGAAACCGCTGGCAACCACTTTTGCCGCGTTTAATTTCTTTCTGAGGCT TATATAAATAGATCAAAGGG GAAAGTGAGATATAATACAGACAAAACAAGAGAAAAGA

Promoter Expression Report #128 Promoter Tested In: Arabidopsis thaliana, Wassilewskija (WS) ecotype Spatial expression summary: Flower H pedicel H receptacle H sepal H petal H filament H anther H carpel H style H stigma H epidermis H stomata H silique Silique H stigma H style H carpel H septum H placentae H epidermis Stem L epidermis L cortex H stomata Leaf H mesophyll H epidermis H trichome H stomata Hypocotyl H epidermis H stomata Cotyledon H mesophyll H epidermis Rosette Leaf H mesophyll H epidermis Primary Root H epidermis Observed expression pattern: Tl mature: Vegetative expression. Not expressed in shoot apical meristem, early flower primordia, pollen and ovules. High expression throughout floral organs. High expression in stem guard cells and cortex cells surrounding stomal chamber. Expression throughout placenta and funiculus but not in any ovule tissue. T2 seedling: Expressed in all tissues near seedling apex increasing toward root. High root epidermis expression. Not expressed in cotyledon and rosette leaf guard cells. Expected expression pattern: Drought inducible Selection Criteria: Ceres, Inc. microarray Gene: Responsive to Dehydration 20 GenBank: NM 128898 Arabidopsis thaliana RD20 protein (At2g33380) mRNA, complete cds gil306856701ref]NM..128898.2|[30685670] Source Promoter Organism: Arabidopsis thaliana, WS ecotype Vector: pNewbin4-HAPl-GFP Marker Type: GFP-ER Generation Screened: XTl Mature XT2 Seedling DT2 Mature D T3 Seedling

Tl Mature Plant Expression Organs/Tissues screened Events Screened: n= 3 Events Expressing: n= 3 GFP Expression Detected X Flower H pedicel H receptacle Dnectary H sepal H petal H filament H anther Dpollen H carpel H style H stigma □ vascular H epidermis H stomata Dtrichome H silique X Silique H stigma H style H carpel H septum H placentae D funiculus D transmitting tissue D vascular H epidermis D stomata D abscission zone D ovule D Ovule Pre-fertilization: Dinner integument D outer integument D embryo sac G funiculus Dchalaza Gmicropyle Dgametophyte Post-fertilization: Dzygote D embryo sack Dinner integument Douter integument D funiculus D endothelium D seed coat Oprimordia Dchalaza Dmicropyle Dearly endosperm Dmature endosperm Dembryo D Embryo Dsuspensor Dpreglobular Dglobular Dheart Dtorpedo Dlate Dmature Dprovascular Dhypophysis Dradicle D cotyledons Dhypocotyl

D Shoot apical meristem | D shoot apical meristem D flower primordium X in the Flower (Fl), of the inflorescence meristem X in the Silique (Si), Stamen (St), Petal (Pe), and Sepal (Se) of the immature flower X in the Silique (Si), Sepal (Se) and Receptacle (Re) of the mature flower X in the Anther (An) X in the Valve margin (Vm) of the silique X in the Funiculus (Fn) of the placenta and mature ovule X in the ovule primordial and developing ovule X in the Trichome (Tc), Epidermis (Ep), Nucleus (Nu) and Guard cells (Gc) X in the Epidermis (Ep), Mesophyll (Me) and Guard cells (Gc) of the abaxial leaf X in the Mesophyll (Me) of the adaxial leaf X in the Cortex (Cr), Epidermis (Ep) and Guard cells (Gc) of thestem

Promoter utility Trait Area: Water stress, Seed Biology, PG&D Sub-trait Area: Drought, Seed fill Utility: Among other uses this promoter sequence can be useful to improve: Valuable as drought-inducible promoter for yield protection in drought or heat stress conditions. Modulation of plant growth rate and architecture. Modulation of growth and development in absence of effects on reproductive cells. Enhancement of source strength and seed filling. Engineering of male sterility. Notes: Takahashi S. Katagiri T. Yamaguchi-Shinozaki K. Shinozaki K. An Arabidopsis gene encoding a Ca2+- binding protein is induced by abscisic acid during dehydration.Plant Cell Physiol. 2000 Jul;41(7):898-903. Yamaguchi-Shinozaki K, Shinozaki K. The plant hormone abscisic acid mediates the drought-induced expression but not the seed-specific expression of rd22, a gene responsive to dehydration stress in Arabidopsis thaliana. MoI Gen Genet. 1993 Apr;238 (l-2):17-25.

Endogenous gene is up-regulated under drought and ABA.

Construct: YP0380 Promoter candidate LD: 11768580 cDNA LD: 12462179 (OCKHAM3-CD) Lines expressing: YP0380 -01, -04, -02

Sequence (SEQ ID NO: 97):

tttgtaatttaaatoctttagttaagttatgattttattatttttgrttatcactta tacga^ gttccagcxjacgtgcatgtgaccacatattgtg^cacagtactcgtcctttttttttct tttgteatcaateaatttcaatcctaaaac^ aaatcataacgagcaaaaaagttcaaatøgggtatatgatcaattgatcatractacat gtctacataa ctaatøgaatøggatgaatcagftcttgcaaacaactacgj^catataatatg^ catgttttgctogcatatgctatcffigagtritrtcctaaactcatagaataagc^ cacgtggatggaccatattaiatgtgatcatattgcttgtcgtcttcgttttcttttaaa tgtttacaccaciBcttcrtgacacgtgtccciBtt^ ggatcacgaacaccaaaacatcaatgtgtacgtc^gcataagaagaaacagagagcatø tcaa g

Promoter Expression Report #129 Promoter Tested In: Arabidopsis thaliana, Wassilewskija (WS) ecotype Spatial expression summary: Flower L pedicel H nectary L epidermis Hypocotyl L vascular Primary Root H vascular Observed expression pattern: Tl mature: High expression in nectary glands of flowers. Low expression in epidermis of pedicels of developing flowers. T2 seedling: GFP expressed in root and hypocotyl vasculature. Expected expression pattern: Drought inducible Selection Criteria: Ceres, Inc. microarray data Gene: Unknown expressed protein GenBank: NM 113878 Arabidopsis thaliana expressed protein (At3g29575) mRNA, complete cds gil30689672|ref|NM 113878.3|[30689672] Source Promoter Organism: Arabidopsis thaliana, Ws ecotype Vector: pNewbin4-HAP 1 -GFP Marker Type: GFP-ER Generation Screened: XTl Mature XT2 Seedling DT2 Mature DT3 Seedling

Tl Mature Plant Expression Organs/Tissues screened Events Screened: n= 2 Events Expressing: n=2 GFP Expression Detected X Flower L pedicel Dreceptacle H nectary D sepal Dpetal D filament □ anther Dpollen G carpel □ style Dpapillae □ vascular L epidermis Dstomata Dtrichome □ silique - D Silique □stigma D style Dcarpel Dseptum Dplacentae Dtransmitting tissue Dvascular □epidermis Dstomata □ abscission zone □ ovule D Ovule Pre-fertilization: Dinner integument □ outer integument □ embryo sac □ funiculus Dchalaza Dmicropyle Dgametophyte Post-fertilization: Dzygote □ embryo sack Dinner integument Douter integument □endothelium Dseed coat Gprimordia Dchalaza Dmicropyle Dearly endosperm □mature endosperm □ embryo D Embryo □suspensor Dpreglobular Dglobular Qheart Dtorpedo Dlate Dmature □provascular Dhypophysis Dradicle Dcotyledons Dhypocotyl D Stem □epidermis Dcortex Dvascular Dxylem Dphloem Dpith Dstomata Dtrichome D Leaf Dpetiole Dmesophyll Dvascular Depidermis Dtrichome Dprimordia Dstomata □stipule □ margin D Shoot apical meristem , □ shoot apical meristem D flower primordium X in the Pedicel (Pd) of the inflorescence meristem X in the lateral and medial Nectary (Ne) in the flower X in the modified stomata opening in the lateral nectary

Promoter utility Trait Area: PG&D Sub-trait Area: Breeding biology Utility: Among other uses this promoter sequence can be useful to improve: Modulation of plant secondary products including volatiles or fragrances. Could be useful to drive genes important in insect attraction for stimulation of outbreeding and hybridization. Useful for making flowerless plants. Modulation of drought stress tolerance. Modulation of heat stress tolerance. Notes: Baum SF, Eshed Y. Bowman JL. The Arabidopsis nectary is an ABC-independent floral structure-Development. 2001 Nov;128(22):4657-67. Endogenous promoter is up-regulated in drought, ABA and far red light.

Construct: YP0381 Promoter candidate I.D: 11768582 cDNA I.D: 12736859 (OCKHAM3-CD) Lines expressing: YP0381 -05, -06

Sequence (SEQ ID NO: 98):

cacggtcaaagtattgctaacatggtrattacattgaaaaagaaaattaattgfc^ actaacaaaatagaaatctøttctaatcacttaattgttgacaattaaatcattg atøcatttaaagttcgacaagttgcgtttacMcatagaaaactaaatctcctaccaaag cgaaatgaaactactaaagcgacaggcaggttacata acctaacaaatctccacgtgtcaattaccaagagaaaaaaagagaagatøagcggaaca cgtggtagcacaaaaaagataatgtgatttaaattaa aaaacaaaaacaaagacacgtgacgacctgacgctgcaacatcccaccttacaacgtaat aaccactgaacataagacacgtgtacgatcttgtct ttgttttctcgatgaaaaccacgtgggtgctcaaagtecttgggfcagagtc^^ gtattttggcttccgcaaattagacaaaacagctttttgtttgattgatttttctcitot ctttttccatc gttogagatttgtcggagattttttcgg^aaatgttgaaattttgtgggatttttttto cattaatcgaagaaatggaatottccaaaatttgataMgctgttttcttgggatttgaat tgctctttatcatcaagaatctgtta^ totøagttgagaaaaagagagatctctaatttaaccggaattaatattctccgaccgaa gttattatgttgcaggct

Tl Mature Plant Expression Organs/Tissues screened Events Screened: n=2 Events Expressing: n=2 GFP Expression Detected X Flower Dpedicel Dreceptacle H nectary M sepal Dpetal Gfilament Danther Dpollen D carpel D style Dpapillae M vascular □ epidermis Dstomata Gtrichome Dsilique D Silique Dstigma Dstyle Dcarpel Dseptum Dplacentae Dtransmitting tissue Dvascular O epidermis Dstomata □ abscission zone D ovule □ Ovule Pre-fertilization: Dinner integument Douter integument Dembryo sac □ funiculus Dchalaza Dmicropyle Dgametophyte Post-fertilization: Dzygote D embryo sack Dinner integument Douter integument Dendothelium Dseed coat Dprimordia Dchalaza Dmicropyle Dearly endosperm Dmature endosperm Dembryo D Embryo Dsuspensor Dpreglobular Dglobular Dheart Dtorpedo Dlate Dmature Dprovascular Dhypophysis Dradicle Dcotyledons Dhypocotyl D Stem Depidermis Dcortex Dvascular Dxylem Ophloem Dpith Dstomata Dtrichome

Induction Screens 1. Drought No differences observed. 2. Drought No differences observed.

Promoter utility Trait Area: PGD, Stress Sub-trait Area: Drought, Root Architecture, Nutrient Uptake Utility: Among other uses this promoter sequence can be useful to improve: Modification of growth and development, especially of flower development. Improvements to nectary tissue and nectar production, and improvements in insect and pollination (outcrossing) biology. Modulation of water and nutrient uptake, loading and transport. Note: Nectaries are secretory structures that produce nectar, a solution composed mainly of sugars. Carpels, stamen, sepals, and nectary vasculature are derived from vascular bundles of the receptacle. Endogenous promoter is down-regulated in shoots, siliques and flowers. Construct: YP0382 Promoter candidate I.D: 11768592 cDNA LD: 12735575 (OCKHAM3-CD) Lines expressing: -04, -05

Sequence (SEQ ID NO: 99):

gcaaacaataatttatcgtaagagtttttttaaaattcgttggaacttggaagggat t^^ aatπaπfitλλiaaaattcgtcgaatttggtaaagcaaaλcggtcgaggatagrt aaiHtttatgcgaaacccgttgtcaaagcagatgttcagcgtcacgcacatgccgcλaa πfiga atatacatcaacctcttttgaacttcacg∞gfttttaggc∞acaal^ ttcttctrtgtgtattgtcgtcagagatgg^ttaaaagttgaaccgaactaiaacgattc tø aatttagg(xaax^gttgtaaMcacaaagaaaatt(^caatttaattcatttaaaaataa agaaaaatttaggtaacaatttaacteagtgg^ctatcteaa^ gaccaaacacaacttaagttgacagccgtdcdctctgttgtttccgtgttattaccgaaa latcagaggaaagtccadaaaxxccaaatattaaa^ aaaggaatctaaattgatcα^cattcgtttcacλcgtttcatatagttg^ ctcaagaaaagtaattctctgtttgtgtagttttctttaccggjgaat^^

Promoter Expression Report #131 .JM' Promoter Tested In: Arabidopsis thaliana, Wassilewskija (WS) ecotype Spatial expression summary: Flower H filament H anther H stomata Silique H ovule Ovule Post-fertilization: H outer integument H seed coat H chalaza Leaf L vascular H stomata Primary Root H epidermis Observed expression pattern: Tl mature: Very high GFP expression levels in stamens of developing flowers. Low expression in vasculature of leaves and guard cells throughout plant. High expression in outer integument of ovules and in seed coats. High incidence of aborted ovules. T2 seedling: Low expression in root epidermal cells. Expected expression pattern: Drought inducible Selection Criteria: Ceres, Inc. Expression data Gene: product="protein phosphatase 2C (PP2C), putative" GenBank: NM 125312 Arabidopsis thaliana protein phosphatase 2C (PP2C), putative (At5g59220) mRNA, complete cds gil306971911ref|NM 125312.2|[30697191] Source Promoter Organism: Arabidopsis thaliana, Ws Vector: pNewbin4-HAPl-GFP Marker Type: GFP-ER Generation Screened: XTl Mature XT2 Seedling DT2 Mature DT3 Seedling

Hypocotyl Epidermis, Vasculature Leaf Epidermis, Vasculature, Guard cells 3. Drought 10-12 days no H20 Flowers Sepals, Epidermis, Guard cells Leaf Epidermis, Mesophyll Stem Epidermis

Tl Mature Plant Expression Organs/Tissues screened Events Screened: n=4 Events Expressing: n=2 GFP Expression Detected X Flower Dpedicel Dreceptacle Dnectary Dsepal Dpetal H filament H anther Dpollen Dcarpel Dstyle Dpapillae Gvascular Depidermis H stomata Dtrichome Dsilique X Silique Dstigma Dstyle Dcarpel Dseptum Dplacentae Dtransmitting tissue Dvascular Depidermis D stomata D abscission zone H ovule X Ovule Pre-fertilization: Dinner integument D outer integument D embryo sac D funiculus □ chalaza Dmicropyle Dgametophyte Post-fertilization: Dzygote D embryo sack Dfuniculus Dinner integument H outer integument D endothelium H seed coat Dprimordia H chalaza Dmicropyle Dearly endosperm Dmature endosperm D embryo D Embryo Dsuspensor Dpreglobular Dglobular Dheart Dtorpedo Dlate Dmature Dprovascular Dhypophysis Dradicle Dcotyledons Dhypocotyl D Stem Depidermis Dcortex Dvascular Dxylem Dphloem Dpith Dstomata Dtrichome

X Leaf Dpetiole D mesophyll L vascular Depidermis Dtrichome Dprimordia H stomata D stipule Dmargin D Shoot apical meristem D shoot apical meristem D flower primordium X in the Guard cells (Gc) and Seed coat (Sc) of the inflorescence meristem X in the Stamen (St) of the flower X in the Seed coat (Sc) of the developing seed X in the Chalaza (Ch) and Outer integument (Oi) of aborted ovules X in the Guard cells (Gc) and Vasculature (Vs) of the leaf

□ Shoot apical meristem | D shoot apical meristetn X in the Epidermis (Ep) of the seedling root

Promoter utility Trait Area: PG&D, Nutrients, Seed Biology Sub-trait Area: Seed size, heat tolerance, drought Utility: Among other uses this promoter sequence can be useful to improve: Modulation of seed size and seed shape by transgene-induced alteration of endosperm loading and/or integument growth. Modulation of flower structure and development especially stamen and anther development. Modulation of root growth and improved water and nutrient loading and transport. Engineering of male sterility and manipulation of stamen and anther development. Improved drought and heat stress tolerance. Improved seedling performance in drought and heat stress conditions. Making longer filaments to improve fertility. Notes: Sulpice R. Tsukaya H. Nonaka H, Mustardy L. Chen TH. Murata N. Enhanced formation of flowers in salt-stressed Arabidopsis after genetic engineering of the synthesis of glycine betaine. Plant J. 2003 Oct;36(2):165-76. Saez A. Apostolova N, Gonzalez-Guzman M. Gonzalez-Garcia MP. Nicolas C. Lorenzo O. Rodriguez PL. Gain-of-function and loss-of-function phenotypes of the protein phosphatase 2C HAB 1 reveal its role as a negative regulator of abscisic acid signalling. Plant J. 2004 Feb;37(3):354-369. Tahtihariu S, Palva T. Antisense inhibition of protein phosphatase 2C accelerates cold acclimation in Arabidopsis thaliana. Plant J. 2001 May;26(4):461-70. Endogenous promoter is induced in drought and drought-like conditions.

Construct: YP0388 Promoter candidate I.D: 11768590 cDNA LD: 13593066 (OCKHAM3-CD) Lines expressing: YP0388 -01, -05

Sequence (SEQ ID NO: 100):

agaaglBttcacgcaccaaggttatatttgtagtgacatattciacaaiMcacattt ttctcttatgtttcgtagtcgcagatggtcaattrffi tttagaaacgatgatatataccgtattgtcacgctcacaatgaaacaaacgcgatgaatc gtcate^ tgtttcxjaacctttctettgaattgattagcagtgatgacgtaattagtgatagtttal ag aggttaagagatctcatgttBtattaaatgattgaaagaaaaacaaactattggttgatt tccaiBtg^ aatfaaaatggfaafffifiaatttcfflca^aaaacitggfaafiaatc^^ gttagactttgattggtgcacglBagtgtttcgtatcgcgatttaggagaagtacgtttt acacgtggacacaatcaacggtcaagatttcgtcgtccagaiBgag cgccattcaacaatct∞tcttcttcattccttcattttgaffigagttttgatctgcc cgttcaaaagtctcggtcatctgcccgtaaaiataaagatga agaagdaatataaagcttccatggctaatcttgtttaagcttctcttcλcttctctdcc tgtgtctcgttcaciagrtttttttcg gatca

Promoter Expression Report # 133 Promoter Tested In: Arabidopsis thaliana, WassilewsMja (WS) ecotype Spatial expression summary: Flower H sepal H petal H anther H style Silique H style H ovule Ovule Prefertilization: H outer integument Postfertilization: H outer integument L seed coat Leaf H vascular Primary Root H epidermis Observed expression pattern: Tl mature: High GFP expression in the style, sepals, petals, and anthers in flowers. Expressed in outer integuments of ovule primordia through developing seed stages and in remnants of aborted ovules. High vasculature expression in leaf. T2 seedling: Medium to low root epidermal expression at root transition zone decreasing toward root tip. Specific to epidermal cells flanking lateral roots. Expected expression pattern: Drought-inducible Selection Criteria: Ceres expression data Gene: PAR-related protein GenBank: NM 124618 Arabidopsis thaliana photoassimilate-responsive protein PAR-related protein (At5g52390) mRNA, complete cds gi[30696178|ref|NM 124618.21[30696178] Source Promoter Organism: Arabidopsis thaliana, Ws ecotype Vector: pNewbin4-HAPl-GFP Marker Type: GFP-ER Generation Screened: XTl Mature XT2 Seedling DT2 Mature DT3 Seedling

Tl Mature Plant Expression Organs/Tissues screened Events Screened: n= 4 Events Expressing: n=3 GFP Expression Detected X Flower Dpedicel Dreceptacle Dnectary H sepal H petal Dfilament H anther Gpollen Dcarpel H style Dpapillae Dvascular Depidermis Dstomata Dtrichome D silique X Silique Dstigma H style Dcarpel Gseptum Dplacentae Dfuniculus D transmitting tissue Dvascular Depidermis Dstomata D abscission zone H ovule X Ovule Pre-fertilization: Dinner integument H outer integument D embryo sac Dfuniculus Dchalaza Dmicropyle Dgametophyte Post-fertilization: D zygote D embryo sack Dinner integument H outer integument Dfuniculus D endothelium L seed coat Dprimordia Dchalaza Dmicropyle Dearly endosperm D mature endosperm D embryo D Embryo Dsuspensor Dpreglobular Dglobular Dheart Dtorpedo Dlate Dmature Dprovascular Dhypόphysis Dradicle Dcotyledons Dhypocotyl D Stem Depidermis Dcortex Dvascular Dxylem Dphloem Dpith Dstomata Dtrichome X Leaf Dpetiole Dmesophyll H vascular Depidermis Gtrichome Dprimordia Dstomata D stipule D margin D Shoot apical meristem D shoot apical meristem D flower primordium X in the Style (Sy) of the Silique (Si) X in the Anther (An), Petal (Pe) and Sepal (Se) of the flower X in the anther locules X in the Outer integument (Oi) of the normal Ovule (Ov) and aborted ovule X in the Hydathode (Hd) and Vasculature (Vs) of the leaf

Promoter utility Trait Area: Abiotic stresses, PG&D Sub-trait Area: Drought, Root architecture Utility: Among other uses this promoter sequence can be useful to improve: Modulation self-incompatability including gametophytic incompatibility. Modulation of flower development and structure. Modulation of seed size and shape. Altered seed uptake of water and mineral ions and an altered seed dormancy and germination. Modulation of drought responses including leaf and flower wilting, ovule abortion, infertility and seed abortion. Notes: Fuiita K. Okada M. Lei K, Ito J, Ohkura K, Adu-Gvamfi JJ. Mohapatra PK. Effect of P-defϊciency on photoassimilate partitioning and rhythmic changes in fruit and stem diameter of tomato (Lycopersicon esculentum) during fruit growth. J Exp Bot. 2003 Nov;54(392):2519-28. Murillo I. Roca R, Bortolotti C, Segundo BS. Engineering photoassimilate partitioning in tobacco plants improves growth and productivity and provides pathogen resistance. Plant J. 2003 Nov;36(3):330-41. The endogenous gene is consistently induced under drought conditions but not surrogate drought treatments.

Construct: YP0396 Promoter candidate LD: 11768788 cDNA LD: 12646726 Lines expressing: YP0396 -02, -03

Sequence (SEQ ID NO: 101):

cafagtaaaagtgaatttaatcatøcfaagtaaaataagataaaacatgtøtttgaai ttgaaM cccgaataaaaatgcataacatgttcttccccaacgaggcgagcgggtragggcactagg gtrattgcaggcagctrataaagtcatgatcatctaggagatcaaattgtatgtcg gccttctcaaaattacctctaagaatctcaaacccaatcategaacctctaaaaagacaa ^ taatttctgaacaacagaattttggatttatttgcacgtatacaaaMctaattaataagg acgactcgtgactatccttacattaagtttcactgtcgaaat^ taatttccacgtrtcaagtctøtaccgtcatttacggagaaagaacatrtctgt^ aaatgatgaccaaatgagaagatggttttgtgccagattttaggaaaagtgagtcaaggt ttcacatctcaaatttgactgcateatcttcgccattaacaacgg∞ ccaattttccatgttgcgtacttttctøttgaggtgaaaatatgggtttgttgattHat caaagagtttgcdaactaatataactacgact^ μlllr.alllylp.Rap.aatattffif^ffiaf^p.aifaaatf^RCrøRRflflRt RtRCRfiftgtRfaRtttt^ aataac

Promoter Expression Report # 135.02 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Flower L anther Observed expression pattern: Tl mature: Low expression in anther walls early in stamen development through pre-dehiscence stage. Not in pollen. T2 seedling: No expression observed. Expected expression pattern: Root hairs Selection Criteria: Mature root exp in endodermis Gene: CYCDl GenBank: NM 105689 Arabidopsis thaliana cyclin delta- 1 (CYCDl) (Atlg70210) mRNA, complete cds gi|306980071reflNM 105689.2|[30698007]. Go function: cyclin-dependent protein kinase regulator Source Promoter Organism: Arabidopsis thaliana, Columbia ecotype Vector: pNewbin4-HAPl-GFP Marker Type: GFP-ER Generation Screened: XTl Mature XT2 Seedling DT2 Mature DT3 Seedling

Tl Mature Plant Expression Organs/Tissues screened Events Screened: n=6 Events Expressing: n=2 GFP Expression Detected X Flower □pedicel Dreceptacle Dnectary Dsepal Dpetal Dfilament L anther Gpollen □carpel □ style Dpapillae □ vascular □ epidermis Dstomata ntrichome □silique D Silique □stigma □ style Dcarpel Qseptum Dplacentae Dtransmitting tissue ^vascular □epidermis Dstomata □ abscission zone □ ovule D Ovule Pre-fertilization: Dprimordia πinner integument □ outer integument □embryo sac □ funiculus Dchalaza Dmicropyle ngametophyte Post-fertilization: □ zygote Osuspensor □ embryo sack □ inner integument □outer integument ^endothelium Dseed coat Dprimordia Dchalaza □micropyle Dearly endosperm Dmature endosperm Dembryo D Embryo □suspensor Dpreglobular Dglobular Dheart Dtorpedo Dlate Dmature □provascular Dhypophysis Dradicle Dcotyledons Dhypocotyl D Stem □epidermis Dcortex Dyascular Dxylem Dphloem Dpith Dstomata Dtrichome D Leaf □petiole πmesophyll avascular Depidermis Qtrichome Dprimordia Dstomata □stipule □ margin D Shoot apical meristem □shoot apical meristem □ flower primordium X in the Filament (Fi)

T2 Seedling Expression Tissues Screened Events Screened: n=2 Events Expressing: n=0 Seedlings expressing / Seedlings screened Event-01: 0/6 Event-02: 0/6 D Scheduled No GFP Expression Detected

Promoter utility Trait Area: PG&D Sub-trait Area: Yield - for hybrid systems Utility: Modulation of anther dehiscense and breeding system. Engineering of male sterility.

Construct: PT0506 Promoter candidate I.D: 11768709 cDNA LD: 13497447 Lines expressing: PT0506 -03, -05

Sequence (SEQ ID NO: 102):

agga<λccacta(λcttcccacacgctccaga<xactgtttgctttcctrtg attaacca^ cttcitccctcacttgatatfacfifiaaaaaafiflctgatttfitcftf^ cattgaatctaaaatggcctcaaatctattgagcatttgtcatgtgcdaaaatggfe^ caaatacttatatalatacgMgattaagcgtcatgactttaaaacrtrtgtaaaalfflg tgtttctttcrtaagcgaccaacaaaatggtccaaatcacagaaaatgttccatøtcca gg ggccttctcMtcacgagtaatatgcaaacaaacgtacattagctgtaatglactcart^ gtttcactttagcgoatgttfigtc^flffflacacaaacataaactacaaafa^ rqqpagtppCgtftfctCgggr^taR^gg^qariggtq^regtrfr^^ tctgtttcttgttta Promoter Expression Report # 136.02 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Flower H filament H anther L vascular Cotyledon L vascular L petiole Primary Root L epidermis Observed expression pattern: Tl mature: High expression at vascular connective tissue between locules of anther. T2 seedling: Low expression in root epidermal cells and vasculature of petioles- Expected expression pattern: Shoot apex including leaf primordia and parts of leaves Selection Criteria: Ceres expression data. Greater than 5x up in stm microarray Gene: Major intrinsic protein (MIP) GenBank: NM 106724 Arabidopsis thaliana major intrinsic protein (MIP) family (Atlg80760) mRNA, complete cds gi]30699534|ref|NM_106724.2|[30699534]. Source Promoter Organism: Arabidopsis thaliana, Columbia ecotype Vector: pNewbin4-HAP 1 -GFP Marker Type: GFP-ER Generation Screened: XTl Mature X T2 Seedling DT2 Mature DT3 Seedling

Tl Mature Plant Expression Organs/Tissues screened Events Screened: n= 2 Events Expressing: n= 2 GFP Expression Detected X Flower Dpedicel Dreceptacle Dnectary Dsepal Dpetal H filament H anther Dpollen □carpel D style Dpapillae L vascular Depidermis Dstomata Dtrichome Dsilique D Silique Dstigma Dstyle Dcarpel Dseptum Dplacentae D transmitting tissue Dvascular Depidermis Dstomata D abscission zone D ovule D Ovule Pre-fertilization: Dprimordia Dinner integument D outer integument D embryo sac D funiculus Dchalaza Dmicropyle Ogametophyte Post-fertilization: Dzygote Dsuspensor D embryo sack Dinner integument D outer integument D endothelium D seed coat Dprimordia Dchalaza Dmicropyle Dearly endosperm Dmature endosperm D embryo D Embryo Dsuspensor Dpreglobular Dglobular Dheart Dtorpedo Dlate Dmature Dprovascular Ohypophysis Dradicle Dcotyledons Dhypocotyl D Stem Depidermis Dcortex Dvascular Dxylem Dphloem Dpith Dstomata Dtrichome D Leaf Dpetiole Dmesophyll Dvascular Depidermis Dtrichome Dprimordia Dstomata D stipule D margin D Shoot apical meristem D shoot apical meristem D flower primordium X in the Anther (An) and Vascular (Vs) of the anther

T2 Seedling Expression Tissues Screened Events Screened: n=2 Events Expressing: n=2 Seedlings expressing / Seedlings screened Event-01: 1/6 Event-02: 3/6 D Scheduled GFP Expression Detected D Hypocotyl Depidermis Dcortex Dvascular Hxylem Dphloem Dstomata

Promoter utility Trait Area: PG&D, Nutrients Sub-trait Area: Drought, Low nitrogen Utility: Modulation of water and nutrient uptake. Modification of seedling vitality and establishment. Modulation of dehiscence properties. Notes: cd00333 MIP: Major intrinsic protein (MIP) superfamily. Members of the MIP superfamily function as membrane channels that selectively transport water, small neutral molecules, and ions out of and between cells. The channel proteins share a' common fold: the N-terminal cytosolic portion followed by six transmembrane ' • helices, which might have arisen through gene duplication. On the basis of sequence similarity and functional characteristics, the superfamily can be subdivided into two major groups: water-selective channels called aquaporins (AQPs) and glycerol uptake facilitators (GIpFs). AQPs are found in all three kingdoms of life, while GIpFs have been characterized only within microorganisms. [cd00333|24161]

Construct: PT0511 Promoter candidate I.D: 11768782 cDNA LD: 12711931 (OCKHAM3-CD) Lines expressing: PT0511 -04, -05

Sequence (SEQ ID NO: 103):

tttaattttgattctaaagagttgtgacgggtcatcacagattcttcgttttttota gatøga agagatgaactcaaaagtgattttaaattttaatgataagaafatacatctcacagaaat cttttatttgacatgtaaaatcttgttttcaccfatcttttgttagtaaacaag gcctcacttggaacgtgataataatatacatctøtcataattgcatattttgcggatag tttttgcatgggga ttalacttgcaaactatacaaccatctgcatcgataattggtgttaatacatgcaaggat tatacactaaaacaaatcatttatttccttacaaaaagagagtcgactgtgagtcacatt ctgt gacaaggaaaggtc^agaaccatcgctttetcatca1t<rtctttgciBacaactta caa(xacacaaacgcaagagttccattctcatggagaagaacatattatgcaaaataatg tat gtcgatcgatagagaaaaggatccacaattBttgctccatotcaaaagcttctttagtac acgatacatgtatcatgtaaatagaaatatgaaagatacaatacacgacccattctcata a agatagcaacatttcatgttatgtaaagagtλccttaggacacatgcattaaaactaag ga ctcataagtcaactdcatgccttcdctaiaaaiB∞gtaccciacgcatcc^ ctctaccctcga

Promoter Expression Report #141 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Flower H stomata Primary Root H pericycle Lateral root H initials Observed expression pattern: Tl mature: Guard cell expression in flowers. T2 seedling: Highly specific GFP expression in pericycle cells at positions of lateral root initiation. Expected expression pattern: Leaf Vasculature high expression Selection Criteria: Mutant lines- CSHL gene trap GT31 Gene: Expressed protein of unknown function (DUF810) . GenBank: NM 120779 Arabidopsis thaliana expressed protein (At5g06970) mRNA, complete cds gil22326640[ref]NM 120779.21[2232664O] Source Promoter Organism: Arabidopsis thaliana WS Vector: pNewbin4-HAPl-GFP Marker Type: GFP-ER Generation Screened: XTl Mature XT2 Seedling DT2 Mature DT3 Seedling

Tl Mature Plant Expression Organs/Tissues screened Events Screened: n= 6 Events Expressing: n= 2 GFP Expression Detected X Flower □pedicel Dreceptacle Dnectary Osepal Dpetal Ofilament Danther Dpollen □carpel D style Dpapillae Avascular Depidermis H stomata Dtrichome □ silique D Silique □ stigma Dstyle Dcarpel Dseptum Dplacentae Dtransmitting tissue Dvascular □ epidermis □ stomata □ abscission zone □ ovule D Ovule Pre-fertilization: Dprimordia Dinner integument D outer integument □ embryo sac □ funiculus Dchalaza Dmicropyle Qgametophyte Post-fertilization: Dzygote Dsuspensor □ embryo sack □ inner integument □ outer integument □ endothelium Dseed coat Dprimordia Dchalaza □micropyle Dearly endosperm D mature endosperm D embryo D Embryo □ suspensor Dpreglobular Dglobular Dheart Dtoφedo Dlate Dmature □provascular Dhypophysis Dradicle Dcotyledons Dhypocotyl D Stem □ epidermis Dcortex Dvascular Dxylem Dphloem Dpith Dstomata Dtrichome D Leaf □petiole Dmesophyll Dvascular Depidermis Dtrichome Dprimordia Dstomata D stipule D margin D Shoot apical nieristem D Shoot apical meristem D Flower primordium X Guard Cells (GC) that define the stomate

H initials D flanking cells D vascular H lateral root cap D Shoot apical meristem D Shoot apical meristem X Lateral root (Lr), Pericycle (Pr)

Promoter utility Trait Area: Water use efficiency, nutrients, seed yield Sub-trait Area: Low nitrogen tolerance, nitrogen use efficiency (20-22), nitrogen utilization, ovule/seed abortion, seed enhancement, seed number, harvest index Utility: Root initiation - regulate number and growth rate of lateral roots to improve lodging tolerance and nutrient acquisition, such as improved nitrate uptake.

Construct: YP0262 Promoter candidate I.D: 11768751 cDNA LD: 12653589 (OCKHAM3-CD) Lines expressing: YP0262 -21, -22

Sequence (SEQ ID NO: 104):

tacttcgtaaggttaatetatgatetagtgggcctgacaaaaaatcata^ gttggctaatctgataccatgtcaaaaaaacatagaagttcttgtgaggtttcttgttta ttcatgtggaggcaattgcccttgtatacaaggcttgtgaaagaagta^ gffiagaaggfggr<TfltgRggg^ff-a3tattr:πtatcλttagtga3λa^ Rattcaaatr^attaagaaataagaffiagfflatflffaaaRagrta^ agttgagac^caattttgacaaattcggtgttcrtggaataacaaaagaagtcaaagaaa g-iaaattBgggagatagagtaaattaaagttgagacaaatcagcttcalLlLgLllLLc cc aaaiaaiaatatacagatagatctttcggccccggcgcattctctagatttgdc^ ttcgtattcagtcgtctctaaattrtcactaaatcaatccttcctttggataa^ attcaaatglagtctctagttctcgtacttgaagtagacttgattcgatø^ ggatcg

Promoter Expression Report #142.02 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Primary Root H epidermis H trichoblast H atrichoblast L root cap H root hairs Observed expression pattern: Tl mature: No expression. T2 seedling: High expression in root epidermal at transition zone decreasing toward root tip. Expected expression pattern: Leaf Selection Criteria: CSHL gene trap GT318 Gene: Glycosyl hydrolase family. GenBank: NM_115876 Arabidopsis thaliana glycosyl hydrolase family 1 (At3g60130) mRNA, complete cds gi|306951301ref]NM 115876.2][306951301 Source Promoter Organism: Arabidopsis thaliana, WS ecotype Vector: pNewbin4-HAPl-GFP Marker Type: GFP-ER Generation Screened: XTl Mature XT2 Seedling DT2 Mature DT3 Seedling

Tl Mature Plant Expression Organs/Tissues screened Events Screened: n=3 Events Expressing: n=0 No GFP Expression Detected T2 Seedling Expression Tissues Screened Events Screened: n=2 Events Expressing: n= 2 Seedlings expressing / Seedlings screened Event-01: 4/6 Event-02: 3/6 □ Scheduled GFP Expression Detected □ Hypocotyl Depidermis Dcortex Dvascular Dxylem Dphloem Dstomata D Cotyledon Dmesophyll Dvascular Depidermis Qmargin Dstomata Dhydathode D Rosette Leaf Dmesophyll Dvascular Depidermis Dtrichome Dpetiole Dprimordia Dstomata D stipule D margin Dhydathode X Primary Root H epidermis H trichoblast H atrichoblast Dcortex Dendodermis Dvascular Dxylern Dphloem Dpericycle Dquiescent D columella L root cap H root hairs D Lateral root Depidermis D trichoblast D atrichoblast Dcortex Dendodermis D initials D flanking cells Dvascular D lateral root cap D Shoot apical meristem D shoot apical meristem X in the seedling root, Epidermis (Ep), Root cap (Rc) and Root hair (Rh) of the root

Promoter utility Trait Area: PG&D, Nutrients Sub-trait Area: Drought, Nutrient uptake Utility: Modulation of root growth rate, size and shape. Modulation of water and nutrient uptake. Modulation of plant-microbe including plant rhizobium interactions. Useful for making insecticidal proteins for protection against root worm.

Construct: YP0275 Promoter candidate I.D: 11768836 cDNA I.D: 12668112 (OCKHAM3-CD) Lines expressing: YP0275 -21, -22

Sequence (SEQ ID NO: 105):

aaacattaaMgtagtaactøgggcgtatgctttactttttaaaatgggccM gggttaagatgacttatttttttacttaccaatttataaatgggcttcgatgtactgaaa taMcgcgcctatt^ ggccctatttcgacatuiHaagaacacctaggtcatcattccagaaatggatattatagg atttagataatttcccacgtttggt^ cgttgaccaacataatcgtgcccaaccgtttcacgcaacgaaiitaiBtacgaaatatat atatttttcaaattaagataccacaatcaaa^ attaacaaagagattttttttttttggttttgagtta^ taagtgttttatttotaatgagtattaatataaataaaacctgcaaaaggataggga^ aattgaaattatgtgaatgttatgtttacaatgaatgattcatc^ tatøcacttattacataacatttatcacatgtgcgtctttttttttttttacffl cttggggctacaccctttctccctccaacaactctatttatagataacati^^ aatttccattataaatcaaaaaataaaaacttaattagtttttacagaagaaaagaaaac a Promoter Expression Report # 143 Promoter Tested In: Arabidopsis thaliana, Wassilewskija (WS) ecotype Spatial expression summary: Primary Root L epidermis L trichoblast L atrichoblast L root hair Observed expression pattern: Tl mature: No expression. T2 seedling: Low expression in root epidermal cells at transition zone decreasing to expression in single cells at mid root. Expected expression pattern: Drought inducible. Selection Criteria: Ceres, Inc. Expression data Gene: Unknown protein. GenBank: NM 101546 Arabidopsis thaliana expressed protein (Atlgl6850) mRNA, complete cds gill8394408|ref1NM 101546.1|[18394408] Source Promoter Organism: Arabidopsis thaliana, WS ecotype Vector: pNewbin4-HAPl-GFP Marker Type: GFP-ER Generation Screened: XTl Mature XT2 Seedling DT2 Mature DT3 Seedling

Tl Mature Plant Expression Organs/Tissues screened Events Screened: n= 3 Events Expressing: n= 0 No GFP Expression Detected

Promoter utility Trait Area: PG&D, Nutrients Sub-trait Area: Drought, Nutrient uptake Utility: Among other uses this promoter sequence can be useful to improve: Modulation of root growth rate, size and shape. Modulation of leaf growth, size and shape. Modulation of water and nutrient uptake. Modulation of plant-microbe including plant rhizobium interactions. Useful for making insecticidal proteins for protection against root worm. Modulation of drought responses and protection against drought and desiccation. Modulation of cold stress responses and protection against cold stress. Notes: This promoter may have multiple utilities, both in plant growth and development and in protection against plant stress, hi stress, the promoter 11768598 is useful for protecting against drought and cold which are something associated (at high latitude and/or high altitude) and sometimes not associated (in temperate regions), suggesting potentially very versatile utility or protection against abiotic stress. The cold-induction can be very valuable since roots are very sensitive to cold stress. The drought induction in the leaf can protect against water loss through the leaf. Endogenous promoter induced in heat and drought as well as surrogate drought treatments.

Construct: YP0337 Promoter candidate LD: 11768598 cDNA LD: 12326510 Lines expressing: YP0337 -01,-02, -03

Sequence (SEQ ID NO: 106):

taatttttttatttttggaacteacacttattagtttaggtttccatcacctattta attcgtaattcttatacat^^ tdcattcattagtatgcattatgcgaaaa∞tcgacgcgcaaaagacacgtaatagcta ataatgttadcattetaatgattgaagcaagacgaaaacaacaacate gtaaactagatatttcttaaaagtgaaaaaaaacaaagaaatataaaggacaattttgag tcagtctcttaatattaaaacatatatacaiaa^ gcaatgtggactttagtttatrtaatcaaaatcaaaataaaaggtgtaatagttctcgtc atttttcaaattttaaaaatcagaacXiaagtg ttaacacagtatatacgtdcttgagatgttgacatgatgataaaatacgagatcgtø^ attagtcacctaatggføcgttgcagtaccgaaccaattttttacccttttfc^ ggitctggtttgaaaaacaagaaataatctgaaagtgtgaaacagcaacgtgtctcggtg tttcatgagccacctgccacctcattcacgtcggtcattttgtcgtttcacggttcacgc t ctegacacgtgctctgtccccaccatgactttcgctgccgactcgctt^

Promoter Expression Report #144 Promoter Tested In: Arabidopsis thaliana, Wassilewskija (WS) ecotype Spatial expression summary: Primary Root H epidermis H trichoblast H atrichoblast Observed expression pattern: Tl mature: No expression. T2 seedling: Root specific GFP expression. High expression throughout root epidermal cells. Expected expression pattern: Drought inducible Selection Criteria: Ceres expression data Gene: Heat shock transcription factor family GenBank: NM 113182 Arabidopsis thaliana heat shock transcription factor family (At3g22830) Source Promoter Organism: Arabidopsis thaliana, WS ecotype Vector: pNewbin4-HAPl-GFP Marker Type: GFP-ER Generation Screened: XTl Mature XT2 Seedling DT2 Mature DT3 Seedling

Tl Mature Plant Expression Organs/Tissues screened Events Screened: n=3 Events Expressing: n=0 No GFP Expression Detected

Promoter utility Trait Area: PG&D, Nutrients, Stress, Pest Protection Sub-trait Area: Drought, Nutrient uptake Utility: Among other uses this promoter sequence can be useful to improve: Modulation of root growth rate, size and shape. Modulation of water and nutrient uptake. Modulation of plant-microbe including plant rhizobium interactions. Useful for making insecticidal proteins for protection against root worm. Protection against drought stress conditions. Protection against heat stress conditions. Notes: Endogenous promoter up-regulated under drought, heat and PEG.

Construct: YP0384 Promoter candidate I.D: 11768599 cDNA LD: 12730108 (OCKHAM3-CD) Lines expressing: YP0384 -01, -02

Sequence (SEQ ID NO: 107):

tttaaaaaattggataaaacaccgataaaaattcacatttgcaaattttattcagtc ggaatato atggatttgttcatctgaaccatgtcg^gattagtgattgat^ccatgaaaaatatgtta tgaaaagtataacaacttttgataaatcacatttattaacaataaatc aacaataatagtagtagaagatettaattcaaattcatccgtaacaacaaaaaatcatac cacaattaa aaaggatatatttgtgtaaaataagaaggatcttgacgggtgtaaaaacatgcacaattc ttaatttagacc^ cttgαtøttttgctøgaatøtatgaagagtgactcatcagggagtggaaaatatrt caggattt^^ trttttaatatgaaaacaacaataatetttciHatagπaaattøaaaagggaaataaa atatttttttaaaatatacaaaagaaga^ facaaacttgtttgcttccttgtdctccctctgtetdcrtca^^ agttgcatcxtttcaaittccactectdctaatøtaattcacattttrc^

Promoter Expression Report #145.02 Promoter Tested In: Arabidopsis thaliana, Ws ecotype Spatial expression summary: Flower H pedicel H receptacle L sepal L epidermis Stem L epidermis L cortex H stomata Primary Root L epidermis Observed expression pattern: Tl mature: High GFP expression in the cortex cells of stems and pedicels of maturing flowers at the inflorescence apex decreasing toward base or mature stem. Low levels of GFP expression in epidermal cells of stem. High magnification of the stem reveals GFP expression within chloroplast containing cells of the cortex. Comparison to previously characterized GFP expression within stem can be seen. GFP-ER marker accumulations within ER membranes near cell walls which reveals cellular morphology while chloroplast cells remain fluorescent red. In this report, the chloroplasts within the cortical cells accumulate GFP. T2 seedling: Low GFP expression in epidermal cells of root near transition zone. Expected expression pattern: Cell growth and differentiation, including the differentiation of root hairs and trichomes. Selection Criteria: Public reference. Gene: Ribosomal protein S5 family GenBank: NM 128939 Arabidopsis thaliana ribosomal protein S5 family (At2g33800) mRNA, complete cdsgil306858421ref|NM 128939.2[[28269302I Source Promoter Organism: Arabidopsis thaliana, Columbia Vector: pNewbin4-HAPl-GFP Marker Type: GFP-ER Generation Screened: XTl Mature XT2 Seedling DT2 Mature DT3 Seedling

Tl Mature Plant Expression Organs/Tissues screened Events Screened: n=4 Events Expressing: n=2 GFP Expression Detected X Flower H pedicel H receptacle Gnectary L sepal Dpetal D filament D anther Dpollen D carpel G style Dpapillae D vascular L epidermis Dstomata Dtrichome Dsilique D Silique Dstigma Dstyle Dcarpel Dseptum Dplacentae Dtransmitting tissue Dvascular D epidermis Dstomata D abscission zone D ovule D Ovule Pre-fertilization: Dprimordia Dinner integument D outer integument D embryo sac ID funiculus Dchalaza Dmicropyle (Hgametophyte Post-fertilization: Dzygote Dsuspensor □ embryo sac Dinner integument Douter integument Dendothelium Dseed coat Dprimordia Dchalaza Dmicropyle Dearly endosperm D mature endosperm D embryo D Embryo Dsuspensor Dpreglobular Dglobular Dheart Dtorpedo Dlate Dmature Dprovascular Dhypophysis Dradicle Dcotyledons Dhypocotyl X Stem L epidermis L cortex Dvascular Dxylem Dphloem Dpith H stomata Dtrichome D Leaf Dpetiole Dmesophyll Dvascular Depidermis Dtrichome Dprimordia Dstomata D stipule Dmargin D Shoot apical meristcm D Shoot apical meristem D Flower primordium X in the Stem (Sm) of the inflorescence meristem X in the Pedicel (Pd) X in the Cortex (Cr) of the Pedicel (Pd) X in the Epidermis (Ep) and Guard cell (Gc) of the stem

Promoter utility Trait Area: Water use efficiency, nutrients Sub-trait Area: Heat, drought, water potential, moisture stress at seed set, moisture stress during seed fill, low nitrogen tolerance, nitrogen use efficiency Notes: Weiiers D, Franke-van Diik M, Vencken RJ, Quint A, Hooykaas P, Offringa R. An Arabidopsis Minute-like phenotype caused by a semi-dominant mutation in a RIBOSOMAL PROTEIN S5 gene. Development. 2001 Nov;128(21):4289-99.

Construct: PT0535 Promoter candidate I.D: 11768627 cDNA LD: 13609001 Lines expressing: PT0535 -03, -05

Sequence (SEQ ID NO: 108):

tatcattattgtactgcctgtcaattttttgattgtatcttttattcagcagcatca cattctccttcagtgtccttctcttcttttctttttcttttctttttctttcctcatta aacccatacaattctacggtaaatattatggatttgcatacaaactctctaattgtttgt ttgggtactataatatggggtccctataatatgtgtagctta actttaagatagaactaagtcaacaagatccggagacaccatgtcaaagttctatgttct tatgattagatgaacaaagttatgatttattcaagatt atcacgattcgaactagttctaaacttgtaaacgtaaaataataaataacgtttgcaact acaacactgaacataaattaatgaagtttgcttgatgt gttggttaaaatttggaatgggacaataaaaagaagaagagaactgagagaagtggtggg ggaagtgggaaccttaacttccccacgtgac actaacgagcaacgttaattgaatagtagtaggccctttttgtagtacactcttttatat atggtcaccttttaattagtttgcaaatcacatacttttaaaa tcataaaagcaattaacgttgctcgttggtgtcatatgcgattgtagttagtataatcga tcttaattaaaacaaagtccatattaaaaatgaccaaa gtatgtaaaaataaaatgttgaattagcatatggtcactgatcttttctattttaagatt tagtaaacctaagatgtatccgcaagcctatagggtttgat gtggattactactagttacttttgtatgggcttgaagaaacttcattgggccacaaagag ggaggagtgagcgaagagcccaaattcaaacaaa aggatatgagggttcaaatgttattataaaaTATAttgtggccaatttgtaaagaaactt gataattactttaaaagccccaacacaattatctttac

Promoter Expression Report #146 Promoter Tested In: Arabidopsis thaliana, Wassilewskija (WS) ecotype Spatial expression summary: Flower L receptacle Silique L abscission zone Primary Root H epidermis Observed expression pattern: Tl mature: Weak GFP expression specific to abscission zone of mature flowers. T2 seedling: Weak expression in root epidermal cells. Expression rapidly decreases from root transition zone to mid root. Expected expression pattern: Drought inducible Selection Criteria: Ceres expression data Gene: Neoxanthin cleavage enzyme GenBank: NM 112304 Arabidopsis thaliana 9-cis-epoxycarotenoid dioxygenase [neoxanthin cleavage enzyme](NCl)(NCEDl), putative (At3gl4440) mRNA, complete cds Source Promoter Organism: Arabidopsis thaliana, WS ecotype Vector: pNewbin4-HAPl-GFP Marker Type: GFP-ER Generation Screened: XTl Mature XT2 Seedling DT2 Mature DT3 Seedling

Tl Mature Plant Expression Organs/Tissues screened Events Screened: n=6 Events Expressing: n=3 GFP Expression Detected X Flower Dpedicel L receptacle Dnectary □ sepal Dpetal D filament D anther Dpollen □carpel Gstyle Dpapillae Dvascular Depidermis Dstomata Dtrichome Dsilique X Silique D stigma D style □ carpel □ septum Dplacentae □ transmitting tissue Dvascular Depidermis Dstomata L abscission zone D ovule D Ovule Pre-fertilization: Dinner integument Douter integument Dembryo sac D funiculus Dchalaza Dmicropyle Dgametophyte Post-fertilization: Dzygote D embryo sack Dinner integument Douter integument D endothelium D seed coat Dprimordia Dchalaza Dmicropyle Dearly endosperm Dmature endosperm Dembryo D Embryo Dsuspensor Dpreglobular Dglobular Dheart Dtorpedo Dlate Dmature Dprovascular Dhypophysis Dradicle Dcotyledons Dhypocotyl D Stem Depidermis Dcortex Dvascular Dxylem Dphloem Dpith Dstomata Dtrichome D Leaf □petiole Dmesophyll Dvascular Depidermis Dtrichome Dprimordia Dstomata □ stipule D margin □ Shoot apical meristem □shoot apical meristem D flower primordium X in the Abscission zone (Az) between the Pedicel (Pd) and Sepal (Se) X in the Abscission zone (Az) in the flower

I X in the Epidermis (Ep) of the seedling root

Promoter utility Trait Area: PG&D, Stress Sub-trait Area: Flower abscission, Drought Utility: Among other uses this promoter sequence can be useful to improve: Delaying or accelerating flower abscission in canola. Delaying or accelerating fruit drop. Making flowerless plants. Modulation of water and mineral nutrient uptake. Useful to drive genes important in corn root worm resistance and other interactions involving roots and pests or microbes. Enhanced tolerance of drought conditions. Enhanced tolerance of heat conditions. Notes: Milborrow BV. The pathway of biosynthesis of abscisic acid in vascular plants: a review of the present state of knowledge of ABA biosynthesis. J Exp Bot. 2001 Jun;52(359):l 145-64. Review. Qin X. Zeevaart JA. The 9-cis-epoxycarotenoid cleavage reaction is the key regulatory step of abscisic acid biosynthesis in water- stressed bean. Proc Natl Acad Sci U S A. 1999 Dec 21;96(26):15354- Endogenous promoter generally up-regulated under drought.

Construct: YP0385 Promoter candidate I.D: 11768579 cDNA I.D: 12658348 (OCKHAM3-C) Lines expressing: YP0385 -02, -03, -06

Sequence (SEQ ID NO: 109):

actcaacaataggacaag∞aaaaaaatecaatøttgtgttactet gtcttaagatattttcatgtgggagatgaataggccaagttggagtctgcaaacaagaag ctcttgagccacgacataagccaagttgatgaccgta attaatgaaactaaatgtgtgtggttatatattagggacccatggccatatacacaa ctaacatatttactggatttgagttcgaatattgacactaatataaactacg^ gttttagaaaattfcaatacacttaacttcatcttacaacggjaaaagaaatcacra^ agagtatacatttøctagatøgagaatttgatatcctcaagttgccaaagaattg aaaagacaaagggagttaaagattgaagtgatgcatttgtctaagtgtgaaaggtctcaa gtctcaactttgaaccataataacattactcacactccc tttttttttctttttttttcccaaagtaccctttttaatte gatccacctttoctcttacccacctcccggtttaiataaacccttcacaacacttcatcg ctctcaaaccaactctctcttctctctto^ agaagaaaaaaaacagagcctttacacatctcaaaatcgaacttactttaaccaccaaat actgattgaacacacttgaaa

Promoter Expression Report #147 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Flower M pedicel M stomata Primary Root L epidermis Observed expression pattern: Tl mature: Weak guard cell expression in pedicles.T2 seedling: Weak root epidermal expression- Expected expression pattern: Drought-Inducible Selection Criteria: Ceres expression data Gene: Unknown protein. Contains putative conserved domains: [ATPase family associated with various cellular activities (AAA). AAA family proteins often perform chaperone-like functions that assist in the assembly, operation, or disassembly of protein complexes] GenBank: NM 179511 Arabidopsis thaliana AAA-type ATPase family protein (Atlg64110) mRNA, complete cds gi] 30696967|ref]NM_ 179511.11 [30696967] Source Promoter Organism: Arabidopsis thaliana, Columbia ecotype Vector: pNewbin4-HAPl-GFP Marker Type: GFP-ER Generation Screened: XTl Mature XT2 Seedling DT2 Mature DT3 Seedling

Tl Mature Plant Expression Organs/Tissues screened Events Screened: n= 6 Events Expressing: n=2 GFP Expression Detected X Flower M pedicel Dreceptacle Dnectary D sepal Dpetal D filament D anther Dpollen Dcarpel Dstyle Dpapillae Dvascular Depidermis M stomata Dtrichome Dsilique D Silique Dstigma Dstyle Dcarpel Dseptum Dplacentae D transmitting tissue Dvascular Depidermis D stomata D abscission zone D ovule D Ovule Pre-fertilization: Dinner integument Douter integument Dembryo sac D funiculus Dchalaza Dmicropyle Dgametophyte Post-fertilization: Dzygote D embryo sack Dinner integument Douter integument Dendothelium Dseed coat Dprimordia Dchalaza Dmicropyle Dearly endosperm D mature endosperm Dembryo D Embryo Dsuspensor Dpreglobular Dglobular Dheart Dtorpedo Dlate Dmature Dprovascular Dhypophysis Dradicle Dcotyledons Dhypocotyl D Stem Depidermis Dcortex Dvascular Dxylem Dphloem Dpith Dstomata Dtrichome D Leaf Dpetiole Dmesophyll Dvascular Depidermis Dtrichome Dprimordia Dstomata D stipule D margin □ Shoot apical meristem D shoot apical meristem D flower primordium X in the Guard cell (GC) of the flower

T2 Seedling Expression Tissues Screened Events Screened: n=2 Events Expressing: n=2 Seedlings expressing / Seedlings screened Event-01: 3/6 Event-02: 2/5 D Scheduled GFP Expression Detected

Promoter utility Trait Area: Nutrients Sub-trait Area: Nutrient uptake, Microbe interactions Utility: Modulate water and nutrient uptake. Modulate seedling establishment. Modulate plant-microbe including plant-Rhizobium interactions.

Construct: YP0371 Promoter candidate I.D: 11768813 cDNA LD: 12657397 (OCKHAM3-CD) Lines expressing: YP0371 -03, -05

Sequence (SEQ ID NO: 110):

gatatatttgtttaataatgcctacgattctgcgaagacaggagaagccataccttt caatctaagccgtcaacttgttcccttacgtgggatecta caatccaacggftctaaatgagccacgccttecagatctaacacagjeatg∞^ tgfgtttaattttgtg∞aacatctataactøcc∞tataaaaa taaagctggactataatgacaattccgaaaciBtcaaggaataaa1iaaagaaactaaaa aactaaagggcattagagtaaagaagcggcaacatc agaattaaaaaactgccgaaaaacxaacctagtagccg^Mgacaacacgtacgcaaagtc tcggtaatgactcatcagttttcatgtgcaaac atattacccccatgaaataaaaaagcagagaagcgatcaaaaaaatottcatlaaaagaa ccctaaatctctoaiatecgccgccgtotttgcctcatt ttcaacaccggfgatgacgtgtaaatagatetgg^cacggttotcactactcte^ aaagaaactgcagaagaaaagatttgagagagatatctøcgaaacaaggføtatattt ct^^ attctcgaataagtoggttaaatagtcaatatagaatøføgataaategatacc^ tttagatcttgatctatatattaataatactaatactttgtLLtαuigULLLlLLllaa tacatatmgcttttggaagatca

Promoter Expression Report # 148.02 I Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Flower L pedicel L receptacle L sepal L carpel L style L stigma L epidermis L stomata L Silique Silique L stigma L style L carpel L epidermis L abscission zone Stem L vascular Leaf L epidermis Hypocotyl L epidermis H vascular Cotyledon L vascular L epidermis Rosette Leaf L epidermis Primary Root H epidermis H cortex H endodermis H vascular H root hairs Lateral root H epidermis H cortex H endodermis H vascular Observed expression pattern: Tl mature: Variable expression throughout epidermal cells of mature flowers and leaves. GFP expression specific to remains of abscised floral organs. Low expression in vasculature of stem. T2 seedling: High GFP expression throughout all root cells decreasing toward root transition zone. High GFP expression in vasculature of root extends to petioles of cotyledons. Low GFP expression throughout seedling epidermis. Expected expression pattern: Flowers and buds Selection Criteria: Ceres expression data Gene: Expressed protein, Unknown function GenBank: NM 105657 Arabidopsis thaliana expressed protein (Atlg69890) mRNA, complete cds gil30697942[reflNM 105657.2j[30697942] Source Promoter Organism: Arabidopsis thaliana, Columbia Vector: pNewbin4-H AP 1 -GFP Marker Type: GFP-ER Generation Screened: XTl Mature XT2 Seedling D T2 Mature D T3 Seedling

Tl Mature Plant Expression Organs/Tissues screened Events Screened: n=5 Events Expressing: n=2 GFP Expression Detected X Flower L pedicel L receptacle Dnectary L sepal Dpetal Dfϊlament Danther Dpollen L carpel L style L stigma Dvascular L epidermis L stomata Dtrichome L silique X Silique L stigma L style L carpel D septum Dplacentae D transmitting tissue D vascular L epidermis D stomata L abscission zone D ovule D Ovule Pre-fertilization: Dprimordia Dinner integument D outer integument D embryo sac D funiculus Dchalaza Dmicropyle Dgametophyte Post-fertilization: Dzygote Dsuspensor D embryo sac Dinner integument □ outer integument D endothelium D seed coat Gprimordia Dchalaza Dmicropyle Dearly endosperm Dmature endosperm Dembryo D Embryo Dsuspensor Dpreglobular Dglobular Dheart Dtorpedo Dlate Dmature Dprovascular Dhypophysis Dradicle Dcotyledons Dhypocotyl X Stem Depidermis Dcortex L vascular Dxylem Dphloem Dpith Dstomata Dtrichome X Leaf Dpetiole Dmesophyll Dvascular L epidermis Dtrichome Dprimordia Dstomata D stipule D margin □ Shoot apical meristem D shoot apical meristem D flower primordium X Epidermis (Ep) and Guard cells (Gc) in the flower X Abscission zone (Az) in the silique X Epidermis (Ep), Guard cells (Gc), and Stigma (Sg) in the pre-fertilized silique X Epidermis (Ep), Guard cells (Gc), and Stigma (Sg) in the fertilized silique X Epidermis (Ep) and Guard cells (Gc) in the leaf X Vasculature (Vs) in the stem J*

T2 Seedling Expression Tissues Screened Events Screened: n=2 Events Expressing: n=2 Seedlings expressing / Seedlings screened Event-01: Event-02: D Scheduled GFP Expression Detected X Hypocotyl L epidermis Gcortex H vascular Dxylem Ophloem Dstomata X Cotyledon Dmesophyll L vascular L epidermis D margin Dstomata Dhydathode X Rosette Leaf Dmesophyll Dvascular L epidermis Dtrichome Dpetiole Dprimordia Dstomata Dstipule Dmargin Dhydathode X Primary Root H epidermis Dtrichoblast Datrichoblast H cortex H endodermis H vascular Dxylem Dphloem Dpericycle Gquiescent D columella Oroot cap H root hairs X Lateral root H epidermis Dtrichoblast Datrichoblast H cortex H endodermis D initials D flanking cells H vascular D lateral root cap D Shoot apical meristem D Shoot apical meristem

Promoter utility Trait Area: Water use efficiency, nutrients, source Sub-trait Area: Heat, drought, water potential, moisture stress at seed set, moisture stress during seed fill, low nitrogen tolerance, nitrogen use efficiency

Construct: PT0610 Promoter candidate LD: 13148293 cDNA LD: 13617251 Lines expressing: PT0610 -01, -05

Sequence (SEQ ID NO: 111):

ttagtgaaatøtgacatiBagfaaggttttcttagttagctaatgtatggctattc aattgt^ caiatgtaaacatcaagatcaagtaacgccattcgagttgatatttttttttt^ aggcaaactaatttgaaacatccgattcgatttcctgtaatttttcttggttaattgacO aaaatcaagatcttcagaaataaaataaaagacgaaagaaagctgtcgcaaagcagattg t gttaaaaaaaagtggattgggcrtcaaacgcaacttgtccagcccgtgacaattacccta iHcgcaagtaagagtocgtatcactggcaaaagttggtattagttacgatatctttgtca tgggggcatgcatgggcatggcttaagagttaagccttaagaagagtcccacactcgtga rt ctatøttcttttggataataacttttctctatataaaataacaaacatcgtacgtttca tttatttacaacaagcgatgagaattaaaaggagacctto caacgggattattacagataatgataatctatatggatgctgacgtggaaaaacaaaatt tggtgaa^^ acatggctatatcatataatacttggatgaattcaaaaiaaacgactgagaaaatgtcca cgtcacggcgcaccgctttggacttaagtctcctataataaaiacaacacca^ attcca

Promoter Expression Report #152 - Promoter Tested In: Arabidopsis thaliana, Wassilewskija (WS) ecotype Spatial expression summary: Flower H stomata Silique H stomata Rosette Leaf H stomata H stipule Primary Root L epidermis L trichoblast H cortex L root hairs Observed expression pattern: Tl mature: Guard cell expression throughout inflorescence apex and carpels in early flower buds. T2 seedling: GFP expression specific within cortex cells overlaying lateral root primordia and root hair producing epidermal cells. Expected expression pattern: Petiole Selection Criteria: Mutant lines Gene: productH'tetratricopeptide repeat (TPR)-containing note="contains Pfam profile PF00515 TPR Domain; go function: protein binding [goid 0005515]" GenBank: NM 129819 tetratricopeptide repeat (TPR)-containing protein (At2g42580) mRNA Source Promoter Organism: Arabidopsis ihaliana, Columbia (Col) ecotype Vector: pNewbin4-HAPl-GFP Marker Type: GFP-ER Generation Screened: X Tl Mature X T2 Seedling DT2 Mature DT3 Seedling

Tl Mature Plant Expression Organs/Tissues screened Events Screened: n=6 Events Expressing: n=2 GFP Expression Detected X Flower Dpedicel Dreceptacle Dnectary Dsepal Dpetal Dfilament Danther Dpollen Dcarpel Dstyle Dpapillae Dvascular Depidermis H stomata Dtrichome Dsilique X Silique Dstigma Dstyle Dcarpel Dseptum Dplacentae D transmitting tissue Dvascular Depidermis H stomata D abscission zone D ovule Ovule Pre-fertilization: Dprimordia Dinner integument D outer integument D embryo sac Dfuniculus Dchalaza Dmicropyle Dgametόphyte Post-fertilization: Dzygote Dsuspensor D embryo sack Dinner integument D outer integument D endothelium D seed coat Dprimordia Dchalaza Dmicropyle Dearly endosperm Dmature endosperm D embryo Embryo Dsuspensor Dpreglobular Dglobular Dheart Dtorpedo Dlate Dmature Dprovascular Dhypophysis Dradicle Dcotyledons Dhypocotyl D Stem Depidermis Dcortex Dvascular Dxylem Dphloem Dpith Dstomata Dtrichome D Leaf Dpetiole Dmesophyll Dvascular Depidermis Dtrichome Dprimordia Dstomata D stipule Dmargin D Shoot apical meristem D shoot apical meristem D flower primordium X Guard cells (Gc) that define the stomata in the flower bud, pedicel and silique

T2 Seedling Expression Tissues Screened Events Screened: n=2 Events Expressing: n=2 Seedlings expressing / Seedlings screened Event-01: 5/6 Event-02: 4/6 D Scheduled GFP Expression Detected D Hypocotyl Depidermis Hcortex Dvascular Dxylem Dphloem Dstomata D Cotyledon Dmesophyll Dvascular Depidermis Dmargin Dstomata Dhydathode X Rosette Leaf Dmesophyll Dvascular Depidermis Dtrichome Dpetiole

Promoter utility Trait Area: Water use efficiency, nutrients Sub-trait Area: Heat, drought, water potential, moisture stress at seed set, moisture stress during seed fill, low nitrogen tolerance, nitrogen use efficiency Utility: Among other uses this promoter sequence could be useful to improve: Drought tolerance through regulation of stomatal closure

Construct: PT0590 Promoter candidate LD: 11768848 cDNA I.D: 12639140 (OCKHAM3-CD) Lines expressing: PT0590 -03, -04

Sequence (SEQ ID NO: 112):

attattcaatttaataaaaattgagtcggccaatttaatgcgagacttctgtacaac gaccctaaaagtgggtttgataaatgaaacatattgc aatagtaacatgtcatggcgcattgaatatccføcgaaggtttagtgtttacttttaaa aaatcc^^ aataaatgatgtatacatttcgatagagtaacattttgggaacatgagtgaacgtgcttg aatcttcgtgc^^ taacagatacaatgtactatatoataattcgtttccgtaacacaacgcaacaatttgaaa g^ ggggttaaatgttttaatttattaagaaaacttaaattcattaaatgttagaaaaagtct aat^ aattaataataaattcgataaatcaccgtattattttcttcaacgacaagtagccgtgaa gacacgggagcgaagagagataaacagaagatgaagaagaagatcaatgtcataatct tcagggagataaatccgtaatctttattaatcaaggttaatcclLlltltltLcttcatc ttaattctttgcgtcttccttttctatttatcacgagatdgtcttt gaagacag^cttgtttctgt∞ggcgttaaaagcttcggtggtggtcλcttgacttct ctgagaagaagaaaaggaagctgagtctcattttagattcagctcacg cga

Promoter Expression Report #154.02 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Flower L pedicel L stomata Ovule Post-fertilization: H suspensor Embryo M suspensor Stem L stomata Leaf L stomata Hypocotyl L stomata Cotyledon L vascular L stomata Primary Root L epidermis H vascular L root cap Observed expression pattern: Tl mature: GFP expression in embryo suspensor cells and root meristem cells in early embryos and in guard cells throughout stems and leaves. T2 seedling: Low GFP expression throughout vasculature tissue of seedlings and in epidermal tissues at root transition zone and root tip, including root cap cells. Expected expression pattern: High in Siliques Selection Criteria: Diversity parameters Gene: Expressed protein GenBank: NM 115605 Arabidopsis thaliana expressed protein (At3g57450) mRNA, complete cds gil42566002]reflNM 115605.2ir425660021 Source Promoter Organism: Arabidopsis thaliana, Columbia Vector: pNewbin4-HAPl-GFP Marker Type: GFP-ER Generation Screened: X Tl Mature X T2 Seedling DT2 Mature DT3 Seedling

Tl Mature Plant Expression Organs/Tissues screened Events Screened: n=2 Events Expressing: n=2 GFP Expression Detected X Flower L pedicel Dreceptacle Dnectary Dsepal Gpetal Ofϊlament Danther Dpollen Dcarpel Dstyle Dpapillae Dvascular Depidermis L stomata Dtrichome Dsilique D Silique Dstigma Dstyle Dcarpel Dseptum Dplacentae D transmitting tissue Dvascular Depidermis D stomata D abscission zone D ovule X Ovule Pre-fertilization: Dprimordia Dinner integument D outer integument D embryo sac D funiculus Dchalaza Dmicropyle Dgametophyte Post-fertilization: D zygote H suspensor D embryo sack Dinner integument Douter integument Dendothelium Dseed coat Dprimordia Dchalaza Dmicropyle Gearly endosperm Dmature endosperm Dembryo X Embryo M suspensor Dpreglobular Dglobular Dheart Dtorpedo Dlate Dmature Dprovascular Dhypophysis Dradicle Dcotyledons Dhypocotyl X Stem Depidermis Dcortex Dvascular Oxylem Dphloem Dpith L stomata Dtrichome X Leaf Dpetiole Dmesophyll Dvascular Depidermis Dtrichome Dprimordia L stomata D stipule Dmargin D Shoot apical meristem D shoot apical meristem D flower primordium

X in the Guard cells (Gc) of the pedicel and leaf, X in the Root apical meristem (RAM) and Suspensor (Su) of the late torpedo stage in the ovule X in the Suspensor (Su) of the ovule

T2 Seedling Expression Tissues Screened Events Screened: n=2 Events Expressing: n=2 Seedlings expressing / Seedlings screened Event-01: 4/6 Event-02: 4/6 D Scheduled GFP Expression Detected X Hypocotyl Depidermis Hcortex Dvascular Dxylem Dphloem L stomata X Cotyledon Dmesophyll L vascular Depidermis Dmargin L stomata Dhydathode D Rosette Leaf Dmesophyll Dvascular Depidermis Dtrichome Dpetiole

Promoter utility Trait Area: Water use efficiency, nutrients Sub-trait Area: Heat, drought, water potential, moisture stress at seed set, moisture stress during seed fill, low nitrogen tolerance, nitrogen use efficiency, seed yield, endosperm cell number/size, endosperm granule number, seed enhancement, seed number, harvest index

Construct: PT0629 Promoter candidate I.D: 13148325 cDNA LD: 12688401 Lines expressing: PT0629

Sequence (SEQ ID NO: 113):

TTTAAGCATATCAAATGTGAAAATTGGTCTTAGGTTATGATGTATATCCTTATATAG TAAAAGTATA TTAGTCTAAAAATTTAGATTTAAAAAGTGAACTAAAATTTAAAAGCAAGCTGAATTACAT AAATCA AGTGACAATTGTAATTTGTTGTGACATTTGTAAGTTCATAATGGTACGTGTCACGCTATG TTAATTA AGTTTGAAATATGAGATGTAAGAAAAAAGTGATCAAAAATTAAGTTCTTTTGGAGCGTTA AGTCCA AAGGTCAATTAGAGCGCAATATCAGTCCAGAAAAGTGGATGAAAAATGTCCATCCATCCA TCGAG AAATATGCTTAAAGTAAACGCTTACTTTTCAAGCGACCTTCACGGTATTGCCGCAGTCAA AAATAC GCGGCGACTGTTTGGTTGGTATAGTGACGACCCACTCTCACGCGGATTCATTAATTTCCT ATCACAA ATAAGAAAAAAGAAACGTGAAATTAATTTGTTAAGTAACAAAAACAAAAGAAAAAGGAAA CTGA CAAGAAACGCTAAAGTGGTTCCTTCCTTGTACTCAAAAAAACGTTAATTTACAGCTCAGA ATAAGT TTTTCTGTTTACCGTAATTTCGACCCAATATTTTTACGAATAACAATTTAGTCCAAAACC TCTTTGAT ATATTGCAATTTAATACCCTAATTAAGTCTTCTTATCTAGTAATTTGGTTTTATTTCCAT GACCTACA ATATAAGATAATAGTCATTAGTGCTAGATATAAAGACTGAATTAGGGTATATAAGATATA TGGAAT GCTAAGATTACAAAGAGATAGAGAATCAAAAAAAGGAAAGAATTAAGGGGCAAATATGAA ATAA TTAAAAACAAGGGAGCCAAAGGAACAATGTAAGAAAAGCAATAAAATTAAAAACAAAACC CTCA AAAAGAAGAAAGCGGAGACTCGTTAGCTTTTTCCTGCCTCCTATAAATTGAACGCGACTT GCAGAA CCTTTCCTCA

Promoter Expression Report # 160.02 Promoter Tested In: Arabidopsis thaliana, WS ecotype Spatial expression summary: Silique L ovule Ovule Post-fertilization: M early endosperm M mature endosperm Hypocotyl L epidermis L stomata Cotyledon L epidermis L stomata Rosette Leaf L epidermis L stomata Primary Root L epidermis Observed expression pattern: Tl mature: Modest GFP expression observed in endosperm from torpedo through to mature embryo-stage ovules. T2 seedling: Low GFP expression throughout seedling epidermal tissues. Expected expression pattern: Embryo-seed Selection Criteria: Public: Rossak, Maren; Smith, Mark; Kunst, Ljerka. Expression of the FAEl gene and FAEl promoter activity in developing seeds of Arabidopsis thaliana. Plant Molecular Biology. August, 2001. 46(6):7l7-725. Gene: Beta-ketoacyl-CoA synthase; FAEl Like GenBank: NM 123743 Arabidopsis thaliana beta-ketoacyl-CoA synthase, putative (At5g43760) mRNA, complete cds gil42568302|reflNM 123743.3|[42568302] Source Promoter Organism: Arabidopsis thaliana,Colυmbia Vector: pNewbin4-HAP 1 -GFP Marker Type: GFP-ER Generation Screened: XTl Mature XT2 Seedling DT2 Mature DT3 Seedling

Tl Mature Plant Expression Organs/Tissues screened Events Screened: n=3 Events Expressing: n=2 GFP Expression Detected D Flower Dpedicel Dreceptacle Dnectary Dsepal Dpetal Dfilament Oanther Clpollen Dcarpel Dstyle Dpapillae Dvascular Depidermis Dstomata Dtrichome Dsilique X Silique D stigma Dstyle D carpel D septum Dplacentae D transmitting tissue Dvascular Depidermis Dstomata D abscission zone L ovule L Ovule Pre-fertilization: Dprimordia Dinner integument D outer integument Dembryo sac Dfuniculus Dchalaza Dmicropyle Dgametophyte Post-fertilization: D zygote Dsuspensor D embryo sac Dinner integument Douter integument Oendothelium Dseed coat Dprimordia Dchalaza Dmicropyle M early endosperm M mature endosperm Dembryo D Embryo Dsuspensor Dpreglobular Dglobular Dheart Dtorpedo Dlate Dmature Dprovascular Dhypophysis Dradicle D cotyledons Dhypocotyl D Stem Depidermis Dcortex Dvascular Dxylem Dphloem Dpith Dstomata Dtrichome D Leaf Dpetiole Dmesophyll Dvascular Depidermis Dtrichome Dprimordia Dstomata D stipule Dmargin D Shoot apical nieristem D shoot apical meristem D flower primordium X in the Endosperm (En) of the ovules at torpedo stage of the embryo and in mature ovules

Promoter utility Trait Area: Nutrients: sink Sub-trait Area: Heat, drought, water potential, moisture stress at seed set, moisture stress during seed fill, low nitrogen tolerance, nitrogen use efficiency, seed yield, endosperm cell number/size, endosperm granule number, seed enhancement, seed number, harvest index, nitrogen use efficiency Utility: Increase seed sink sterength to improve seed quality, protein content and oil content.

Construct: PT0642 Promoter candidate LD: 11768742 cDNA LD: 13612923 Lines expressing: PT0642 -03, -05

Sequence (SEQ ID NO: 114):

ttgaacatccttaatttgaaccataaaaaatatgacaπaactatctgattaatttt cacttaagggatggttagttaagttacattggattaaaaatggt attagtagaccaattagaacatgtatgccattttttgtttacaaaaacctttttaagtgg tatttataagacttgctcaatttcattcaaaagaacatgaa atggatggactagttttaatggcaataacccacacaattactcatatttggttcaacaaa cttctatttcggttcacttattaattttccatctatatatgaa tcatataatatgattaatttacacaagttcacacggccacgtaaaatgtaactgtcttca agttgtgcacataaagagggtagtttcgaagataacg ggtcaacgaaagggtaaaagagtaaattgcatagaacgcggGcaaattaaaagcccGcaa ttgggataaaagtcatcgccgtctcttaggtgt caaatctcaactgtctaaaaacattaaaagcttcgttggggtagttgcatcttccctctc taatacaaaacttattttatccagttttagtttcgatttttcatt tgttaacatgttttcatttttcttttaatgttaatagcctttttatttggaaaatgaaaa caatttcacaatttaattctaaattaccatttctaaaaaatagaaa taatataaataataatattgaataatacatggactaaaaaattatagtactgtcctaaac aaaattgcttgactagattgaacagaaaatgtttttgat gtcttactagtttgaacaatttatttgctaacattattctctttgtatatttcttaaaaa cccatattttttccttaaatatttcccatttcccctaactacattcaat agctaagtctctctctccctctctctttctctctcactcaaaaatttcccattaaattct caaattttctccaactttttaggcca

Promoter Expression Report #161 Promoter Tested In: Arabidopsis thaliana, Wassilewskija (WS) ecotype Spatial expression summary: Flower H pedicel H sepal H petal H filament H anther H carpel H style H epidermis H stomata H silique Silique H style H carpel H transmitting tissue H epidermis H ovule Ovule Pre-fertilization: L funiculus L outer integument Post-fertilization: H funiculus H outer integument H seed coat Stem L vascular L phloem Leaf L vascular Hypocotyl H epidermis L vascular Cotyledon L epidermis Rosette Leaf L vascular L epidermis Primary Root H epidermis H cortex H endodermis H vascular H quiescent H root cap H root hairs Observed expression pattern: Tl mature: GFP expression in sepals, petals, stamens and siliques of developing floral buds through to mature flowers. GFP expression throughout all tissues of stamen excluding pollen. GFP expression throughout all tissues of silique excluding stigma. Within ovules, highest GFP expression is at funiculus, outer integument and mature seed coat. GFP expression in vascular tissues of flowers, stems and leaves. In stem, expression in phloem cells within vascular bundle. Expression in guard cells throughout plant. T2 seedling: High GFP expression throughout epidermal tissues of seedlings. High GFP expression throughout all root cell types decreasing toward elongation zone. GFP is expressed in root cap and meristem cells. Expected expression pattern: High in siliques Selection Criteria: Microarray data Gene: Chlorophyll A-B binding family protein / early light-induced protein GenBank: NM 113183 Arabidopsis thaliana chlorophyll A-B binding family protein / early light-induced protein (ELIP) (At3g22840) mRNA, complete cds gi|30686801| Source Promoter Organism: Arabidopsis thaliana, Columbia (Col) ecotype Vector: pNewbin4-HAPl-GFP Marker Type: GFP-ER Generation Screened: X Tl Mature X T2 Seedling D T2 Mature D T3 Seedling

Tl Mature Plant Expression Organs/Tissues screened Events Screened: n= 6 Events Expressing: n=3 GFP Expression Detected X Flower H pedicel Dreceptacle Dnectary H sepal H petal H filament H anther Dpollen H carpel H style Dpapillae Dvascular H epidermis H stomata Dtrichome H silique X Silique D stigma H style H carpel D septum D placentae H transmitting tissue Dvascular H epidermis G stomata □ abscission zone H ovule X Ovule Pre-fertilization: Dprimordia Dinner integument L outer integument D embryo sac L funiculus Dchalaza Gmicropyle Dgametophyte Post-fertilization: Dzygote Dsuspensor D embryo sac H funiculus Dinner integument H outer integument D endothelium H seed coat Dprimordia Dchalaza Dmicropyle Dearly endosperm D mature endosperm D embryo D Embryo Dsuspensor Dpreglobular Dglobular Dheart Dtorpedo Dlate Gmature Dprovascular Ghypophysis Dradicle Dcotyledons Dhypocotyl X Stem D epidermis D cortex L vascular Dxylem L phloem Dpith D stomata Dtrichome

X Leaf Dpetiole Dmesophyll L vascular Depidermis Dtrichome Dprimordia Dstomata D stipule D margin D Shoot apical meristem D shoot apical meristem D flower primordium X Pedicel (Pd) and Stem (Sm) in the inflorescence meristem X Pedicel (Pd) and flower bud in the inflorescence meristem X Sepal (Se) in the flower X Pollen (Po), Petal (Pe) and Stamen (St) in the stamen X Pollen (Po), Ovule (Ov) and Stigma (Sg) in the pre-fertilized silique X Stigma (Sg) and Pollen transmitting tract (Tt) in the unfertilized stigma X Funiculus (Fn) in the pre-fertilized ovule X Funiculus (Fn) and Chalaza (Ch) in the fertilized ovule X Funiculus (Fn), Placenta (Pl) and Outer Integument (Oi) in the developing seed X and Outer Integument (Oi) in the developing seed X Seed coat (Sc) in the early mature seed X in the Seed coat (Sc) X Columella (Cm), Radial wall (Rw) and Starch granule (Sg) in the seed coat X in the embryo X Guard cell (Gc), Vasculature (Vs) and Hydathode (Hd) in the Leaf X in the stem

Promoter utility Trait Area: Nutrients, seed yield, water use efficiency Sub-trait Area: Nitrogen use efficiency, ovule/seed abortion, endosperm cell number/size, endosperm granule number/size, seed enhancement, seed number, harvest index, heat, water potential, drought, moisture stress at seed set Utility: Among other uses this promoter sequence could be useful to improve: Notes: This promoter is strongly differentially regulated under drought conditions and in a number of tissues. Construct: PT0623 Promoter candidate I.D: 11768718 cDNA LD: 23644072 Lines expressing: PT0623 -01,-05, -06

Sequence (SEQ ID NO: 115):

aaagttattgacattttgaaaggaccgtaaatattaccaaaaaactgacggagttag gatcggccacgtagaaagggacaaagagagaacagtoacggactcggcxiagactaagt atgggcctgtctgaatccaaactcagctaagttccaaaagcataaagagagatgtgtaat gaaatgaac^ gggagatggagagaatcttttttacgtttttaacdaacccacttggcacttg^ caaaagttaaatttatgttatgcgtggggacaatctaagcaacgtggttcctttaaatat cgcagctte^ gtcagtcagtttagaaataatttggatgtccaaaaatcttggagatccaaataaaataat tagcatgttttagttcataagaatatgaaatgtagataaactgtc^ attggctttttatcgaggtgatgtacttaatgactttgttgattactactcgtata^

gfgtgRRCtRtflttRRCftRtCRRtRtOgRRRggCtRRRtRRgtRRRtRRrtRRRR gRRRgttCRggRRRCRRCtC^^ cgtaagatcattcgttact

Promoter Expression Report #163 Promoter Tested In: Arabidopsis thaliana, Wassilewskija (WS) ecotype Spatial expression summary: Flower H pollen Silique M ovule Ovule Post-fertilization: M inner integument M endothelium M embryo Embryo M mature embryo H root tip L radicle Stem L vascular Primary Root H epidermis H cortex L root cap H root hairs Observed expression pattern: Tl mature: High GFP expression in endothelium cell layer of ovules and root cap of embryos. GFP expressed in pollen. Low GFP expression in stem vascular tissue. T2 seedling: High GFP expression specific to epidermal cells and root cap. GFP expression in epidermal cells decreases toward root elongation zone where no expression is observed. Expected expression pattern: Flowers and buds Selection Criteria: Microarray data Gene: product="expressed protein"/note="similar to myo-inositol oxygenase GenBank: NM 127538 Arabidopsis thaliana expressed protein (At2gl9800) mRNA, complete Source Promoter Organism: Arabidopsis thaliana, Columbia (Col) ecotype Vector: pNewbin4-HAPl-GFP Marker Type: GFP-ER Generation Screened: XTl Mature XT2 Seedling DT2 Mature DT3 Seedling

Tl Mature Plant Expression Organs/Tissues screened Events Screened: n=4 Events Expressing: n=2

Promoter utility Trait Area: Seed, yield, nutrients Sub-trait Area: Seed enhancement, harvest index, nitrogen use efficiency Nominated By: Diversity parameters Investigators: Medrano, L., Theiss, N. Utility: Among other uses this promoter sequence could be useful to improve: Seed enhancement, nutrient uptake and thus yield. Notes: Lorence A. Chevone BI. Mendes P. Nessler CL. myo-inositol oxygenase offers a possible entry point into plant ascorbate biosynthesis. Plant Physiol. 2004 Mar;134(3):1200-5. Epub 2004 Feb 19.

Construct: PT0613 Promoter candidate I.D: 13148297 cDNA I.D: 23555688 Lines expressing: PT0613 -01,-04

Sequence (SEQ ID NO: 116):

ttaatactaacattgtagaaagccacaaaaaagaaattgaaatgtgagtagatgcλ gagtcagaggtttggtcaatacacaacagctaattgagataatattatacacgtcacgat gact tgttttttctcctcccaacttgttaatttctttattcttaaaato tcccaattcaggactccttagattttcctaaatttgttttcctaacttgctctctctcat tctaacattttctcattttttega ttgar^tt^^Rqggg^ttcta3a33ttc3ffi£tSSfl^tgrtgat^ faatcgatrcWgnl 1111 IgI 113gaf 3aaafffijffflC3g(Mritatcl 11111111 IgI I Rt^gfflttttPFitcgλfitiiR3Pg^g3λ33Rtfi^^3gltfit!itcttc1tct! 111tjifictλfitgfiλficλg ttatatcttaaacaaacaacagaaacagtaaaatattaatgcaaatccgcgtcaagagat aaattttaacaaactaataacaattgagataagattagcgcaaaagaaactctaatttta g agcgtgtaaacacaaacacgtcttgaaagtaaacgtgaatøcacgcttctaaaacgagc tttgaggacacaactcaagttatgtttgaiatctaaggacttgcactgtctccaaatctg c^^

Promoter Expression Report #261 Report Date: March 24, 2005

Promoter Tested In: Arabidopsis thaliana, Wassilewskija (WS) ecotype Spatial expression summary: Tables 1.1, 2.1. Flower L pedicel L receptacle L vascular Silique L placenta L vascular Ovule Post-fertilization: L chalaza Hypocotyl L vascular Cotyledon L vascular Rosette Leaf L vascular Primary Root H vascular H pericycle

Observed expression patterns: Tl mature: GFP expressed in vasculature of the receptacle, placenta, and ovules of flowers. T2 Seedling: GFP highly expressed throughout vasculature of root decreasing toward shoot and root apex. Weak GFP expression in vasculature of cotyledons and rosette leaves. Expected expression pattern: Phloem of the stem; xylem-to-phloem transfer tissues and in the veins of supplying seeds. Vascular strands of siliques and in funiculi. Also expressed in the vascular system of the cotyledons in devoping seedlings. Selection Criteria: Arabidopsis public PNAS 92, 12036-12040 (1995) Gene: AAP2 (X95623) GenBank: NM 120958 Arabidopsis thaliana amino acid permease 2 (AAP2) (At5g09220) Source Promoter Organism: Arabidopsis thaliana, Wassilewskija (WS) ecotype Vector: pNewbin4-HAPl-GFP Marker Type: X GFP-ER Generation Screened: X Tl Mature X T2 Seedling D T3 Mature D T3 Seedling Mature Plant Expression Organs/Tissues screened Events Screened: n=3 Events Expressing: n=2 GFP Expression Detected X Flower L pedicel L receptacle D nectary D sepal Gpetal D filament D anther Dpollen Dcarpel Dstyle Dpapillae L vascular Depidermis Dstomata Dtrichome Dsilique X Silique O stigma Dstyle Dcarpel D septum L placenta D funiculus D transmitting tissue L vascular Depidermis Dstomata D abscission zone D ovule X Ovule Pre-fertilization: Dprimordia Dinner integument D outer integument D embryo sac D funiculus Dchalaza Dmicropyle Dgametophyte Post-fertilization: D zygote Dsuspensor D embryo sack D funiculus Dinner integument D outer integument D endothelium D seed coat Dprimordia L chalaza Dmicropyle Dearly endosperm Dmature endosperm Dembryo Embryo Dsuspensor Gpreglobular Dglobular Dheart Dtorpedo Dlate mature Dprovascular Dhypophysis Dradicle Dcotyledons Droot meristem Dshoot meristem D Stem Depidermis Dcortex D interfascicular region Dvascular Dxylem Dphloem Dpith Dstomata Dtrichome D Leaf Dpetiole Dmesophyll Dvascular Depidermis Dtrichome Dprimordia Dstomata D stipule D margin D Shoot apical meristem D Shoot apical meristem D Flower primordium X in the vasculature (Vs) of the flower X in the Placenta (Pl) and Funiculus (Fn) adjacent to the ovule attachment site X in the Chalaza (Ch) of the ovule

Promoter utility Trait Area: Nutrient Sub-trait Area: Storage in roots, uptake of minerals Utility: Among other uses this promoter sequence could be useful to improve: Seed - Seed enhancement Source - transport amino acids Yield - harvest index, test weight, seed size, Quality - amino acids, carbohydrate, protein, total seed composition

Construct: YP0093 Promoter Candidate LD: 13148232 cDNA ID: 23617640 Lines expressing: YP0093 -01,-02, -03

Sequence(SEQIDNO: 117): 5 atgatgaacattctacatatataattattatgtttaagcacttagacagcataaattctt tctaattatataaat ctaaccttgttacattgtacatctataaattacttgaagaaataacgagttctatttctt tttaaaaattaaaaa tactataccatatctcagtgattaagttgaaccaaaaggtacggaggagaaacaagcatt tgattcttccttatt ttattttattcatctctcactaatgatggtggagaaaaaaagaaaatacctaacaaacaa atatatattgtcata - • ■-caaaaatatttctatatttttagttaattagtttatattcctcacttttcagggct tatataagaaagtgagcaa 10 acacaaatcaaaatgcagcagcaaatactatcatcacccatctccttagttctattttat aattcctcttctttt tgttcatagctttgtaattatagtcttatttctctttaaggctcaataagaggaggtact attactacacttctc tctacttttacttgtattttagcattaaaatcctaaaatccgttttaaattcaaaaataa acttagagatgttta atctcgattcggtttttcggctttaggagaataattatatgaaattagtatggatatctt tactagtttccattc aaatgattctgatttcaatctaatactctcactctttaattaaactatatgtagtgtaat ttcacactgttaaat 15 ttctaccatgtcatgtatattagagttgcatagaaaattgtaaaacatccatttgaattc gaatgaaacaaaatg ttttaaaataaaattttggtttttaaaagaaaaatctaaaactgaattatatcgtttaac caagttgtaaaagtc ataaaacgtagtatcttgtaaatcgctcttccacggtccaaatagacttctagtaataaa caagtaaaactaatt ttggtttcttactaattttcacaga

20 Table 2 Legend for Table 2 The invention being thus described, it will be apparent to one of ordinary skill in the art that various modifications of the materials and methods for practicing the invention can be made. Such modifications are to be considered within the scope of the invention as defined by the following claims. Each of the references from the patent and periodical literature cited herein is hereby expressly incorporated in its entirety by such citation.