Title:
PROTEIN IMMOBILIZATION METHOD AND QUANTIFICATION METHOD
Document Type and Number:
WIPO Patent Application WO/2005/070969
Kind Code:
A1
Abstract:
It is intended to provide a method of immobilizing a protein on a solid phase characterized by comprising bringing the protein into contact with the solid phase having a hydrophobic surface in the coexistence of a lower alcohol with a halogenated carboxylic acid and/or a long-chain alkyl sulfuric acid salt; an immobilization reagent to be used therein; a method of quantifying a protein characterized by comprising bringing a solid phase having the protein immobilized thereon by the immobilization method as described above into contact with a protein-staining solution and quantifying the protein depending on the degree of coloration thus induced; and an immunoblotting method characterized by comprising using a solid phase having a protein immobilized thereon by the immobilization method as described above. It is also intended to provide a method of detecting abnormal PrP quickly and rapidly compared with the existing method; and a method of judging BSE.
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Inventors:
KOHNO NAOYUKI (JP)
UEMORI HITOSHI (JP)
NISHIBU TAKAHIRO (JP)
HIRAYASU KAZUNARI (JP)
KOBAYASHI YOSHITERU (JP)
YOKOYAMA TAKASHI (JP)
UEMORI HITOSHI (JP)
NISHIBU TAKAHIRO (JP)
HIRAYASU KAZUNARI (JP)
KOBAYASHI YOSHITERU (JP)
YOKOYAMA TAKASHI (JP)
Application Number:
PCT/JP2005/000737
Publication Date:
August 04, 2005
Filing Date:
January 21, 2005
Export Citation:
Assignee:
WAKO PURE CHEM IND LTD (JP)
NAT AGRICULTURE & BIO ORIENTED RES ORG (JP)
KOHNO NAOYUKI (JP)
UEMORI HITOSHI (JP)
NISHIBU TAKAHIRO (JP)
HIRAYASU KAZUNARI (JP)
KOBAYASHI YOSHITERU (JP)
YOKOYAMA TAKASHI (JP)
NAT AGRICULTURE & BIO ORIENTED RES ORG (JP)
KOHNO NAOYUKI (JP)
UEMORI HITOSHI (JP)
NISHIBU TAKAHIRO (JP)
HIRAYASU KAZUNARI (JP)
KOBAYASHI YOSHITERU (JP)
YOKOYAMA TAKASHI (JP)
International Classes:
C07K17/02; G01N33/53; G01N33/68; (IPC1-7): C07K17/02; G01N33/53
Domestic Patent References:
| WO2002057793A2 | 2002-07-25 |
Foreign References:
| JPS58205855A | 1983-11-30 | |||
| JP2002236127A | 2002-08-23 |
Other References:
GONCALVES C.A. ET AL.: "Electrotransfer of fixed phosphoproteins from piece of dried polyacrylamide to small disks of nitrocellulose, nylon of polyvinylidene difluoride", ELECTROPHORESIS, vol. 14, no. 8, 1993, pages 789 - 793, XP002976461
JACOBSON G. ET AL.: "Important parameters in semi-dry electrophoretic transfer", ELECTROPHORESIS, vol. 11, no. 1, 1990, pages 46 - 52, XP002976462
SHEFFIELD J.B. ET AL.: "A solid-phase method for the quantitation of protein in the presence of sodium dodecyl sulfate and other interfering substances", ANALYTICAL BIOCHEMISTRY, vol. 166, no. 1, 1987, pages 49 - 54, XP002976463
YAMAGUCHI K. ET L.: "Immunostaining method of soferritins separated by isoelectric focusing on cellulose acetate membrane", JAPANSES JOURNAL OF ELECTROPHORESIS, vol. 35, no. 4, 1991, pages 275 - 277, XP002976464
See also references of EP 1712567A4
JACOBSON G. ET AL.: "Important parameters in semi-dry electrophoretic transfer", ELECTROPHORESIS, vol. 11, no. 1, 1990, pages 46 - 52, XP002976462
SHEFFIELD J.B. ET AL.: "A solid-phase method for the quantitation of protein in the presence of sodium dodecyl sulfate and other interfering substances", ANALYTICAL BIOCHEMISTRY, vol. 166, no. 1, 1987, pages 49 - 54, XP002976463
YAMAGUCHI K. ET L.: "Immunostaining method of soferritins separated by isoelectric focusing on cellulose acetate membrane", JAPANSES JOURNAL OF ELECTROPHORESIS, vol. 35, no. 4, 1991, pages 275 - 277, XP002976464
See also references of EP 1712567A4
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