Title:
PROTEIN OR PEPTIDE PRINTING METHOD, PROTEIN OR PEPTIDE ARRAY AND FUNCTIONAL PROTEIN OR FUNCTIONAL PEPTIDE IDENTIFICATION METHOD
Document Type and Number:
WIPO Patent Application WO/2012/026541
Kind Code:
A1
Abstract:
Disclosed are: (a) a step where, on a micro-engraved plate that consists of microscopic grooves that have a specific mouth shape, nucleic acids and an acellular protein synthesis system are prepared within said microscopic grooves; (b) a step where a substrate is layered on top of the micro-engraved plate in such a manner that, after synthesis within the microscopic grooves, the proteins or peptides are in contact with the substrate; and (c) a step where, in the microscopic grooves, the acellular protein synthesis system is used and proteins or peptides are synthesised from the nucleic acids and the proteins or peptides are fixed to the substrate along the specific mouth shapes of the microscopic grooves.
Inventors:
ICHIKI TAKANORI (JP)
BIYANI MANISH (JP)
SHIONO HIROFUMI (JP)
BIYANI MANISH (JP)
SHIONO HIROFUMI (JP)
Application Number:
PCT/JP2011/069210
Publication Date:
March 01, 2012
Filing Date:
August 25, 2011
Export Citation:
Assignee:
UNIV TOKYO (JP)
NIKON CORP (JP)
ICHIKI TAKANORI (JP)
BIYANI MANISH (JP)
SHIONO HIROFUMI (JP)
NIKON CORP (JP)
ICHIKI TAKANORI (JP)
BIYANI MANISH (JP)
SHIONO HIROFUMI (JP)
International Classes:
C12N15/00; C07K17/14; C12P21/02; G01N37/00
Domestic Patent References:
| WO2008053598A1 | 2008-05-08 | |||
| WO2007046520A1 | 2007-04-26 |
Other References:
HE, M. ET AL.: "In situ synthesis of protein arrays.", CURR. OPIN. BIOTECHNOL., vol. 19, no. 1, 2008, pages 4 - 9
BLATTLER, T. ET AL.: "Nanopatterns with biological functions.", J. NANOSCI. NANOTECHNOL., vol. 6, no. 8, 2006, pages 2237 - 2264
BIYANI, M. ET AL.: "Microintaglio printing of biomolecules and its application to in situ production of messenger ribonucleic acid display microarray.", APPL. PHYS. EXPRESS., vol. 4, no. 4, 25 April 2011 (2011-04-25), pages 047001.1 - 047001.3
BLATTLER, T. ET AL.: "Nanopatterns with biological functions.", J. NANOSCI. NANOTECHNOL., vol. 6, no. 8, 2006, pages 2237 - 2264
BIYANI, M. ET AL.: "Microintaglio printing of biomolecules and its application to in situ production of messenger ribonucleic acid display microarray.", APPL. PHYS. EXPRESS., vol. 4, no. 4, 25 April 2011 (2011-04-25), pages 047001.1 - 047001.3
Attorney, Agent or Firm:
SHIGA Masatake et al. (JP)
Masatake Shiga (JP)
Masatake Shiga (JP)
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