Title:
PROTEIN WITH GLUCOCEREBROSIDASE ACTIVITY AND PRODUCTION METHOD THEREFOR
Document Type and Number:
WIPO Patent Application WO/2023/145813
Kind Code:
A1
Abstract:
The present invention provides a protein that is able to prevent viral infection and has glucocerebrosidase activity. The protein (a) contains an amino acid sequence represented by SEQ ID NO: 1 or 2, or an amino acid sequence having at least 90% identity with the amino acid sequence represented by SEQ ID NO: 1 or 2, (b) does not have an added sugar chain, and (c) has glucocerebrosidase activity.
Inventors:
TATEIWA DAISUKE (JP)
MAKINO YUKI (JP)
MAKINO YUKI (JP)
Application Number:
PCT/JP2023/002410
Publication Date:
August 03, 2023
Filing Date:
January 26, 2023
Export Citation:
Assignee:
NIPPON CATALYTIC CHEM IND (JP)
International Classes:
C12N15/56; C12N9/24; C12P21/02
Foreign References:
| JP2014500722A | 2014-01-16 |
Other References:
NOVO JULIANA BRANCO, MORGANTI LIGIA, MORO ANA MARIA, PAES LEME ADRIANA FRANCO, SERRANO SOLANGE MARIA DE TOLEDO, RAW ISAIAS, HO PAU: "Generation of a Chinese Hamster Ovary Cell Line Producing Recombinant Human Glucocerebrosidase", JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY, vol. 2012, 1 January 2012 (2012-01-01), pages 1 - 10, XP093082738, ISSN: 1110-7243, DOI: 10.1155/2012/875383
TEKOAH YORAM, TZABAN SALIT, KIZHNER TALI, HAINRICHSON MARIANA, GANTMAN ANNA, GOLEMBO MYRIAM, AVIEZER DAVID, SHAALTIEL YOSEPH: "Glycosylation and functionality of recombinant β-glucocerebrosidase from various production systems", CELL DEATH AND DISEASE, vol. 33, no. 5, 1 October 2013 (2013-10-01), pages 771 - 781+Supp, XP093082752, ISSN: 0144-8463, DOI: 10.1042/BSR20130081
GRACE, M.E. ; GRABOWSKI, G.A.: "Human acid ^2-glucosidase: Glycosylation is required for catalytic activity", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ELSEVIER, AMSTERDAM NL, vol. 168, no. 2, 30 April 1990 (1990-04-30), Amsterdam NL , pages 771 - 777, XP026787120, ISSN: 0006-291X, DOI: 10.1016/0006-291X(90)92388-G
BERG-FUSSMAN A ET AL: "Human acid beta-glucosidase. N-glycosylation site occupancy and the effect of glycosylation on enzymatic activity.", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, US, vol. 268, no. 20, 15 July 1993 (1993-07-15), US , pages 14861 - 14866, XP002436428, ISSN: 0021-9258
POL-FACHIN LAERCIO; SIEBERT MARINA; VERLI HUGO; SARAIVA-PEREIRA MARIA LUIZA: "Glycosylation is crucial for a proper catalytic site organization in human glucocerebrosidase", GLYCOCONJUGATE JOURNAL, CHAPMAN & HALL, BOSTON, vol. 33, no. 2, 29 March 2016 (2016-03-29), Boston , pages 237 - 244, XP035909603, ISSN: 0282-0080, DOI: 10.1007/s10719-016-9661-7
TEKOAH YORAM, TZABAN SALIT, KIZHNER TALI, HAINRICHSON MARIANA, GANTMAN ANNA, GOLEMBO MYRIAM, AVIEZER DAVID, SHAALTIEL YOSEPH: "Glycosylation and functionality of recombinant β-glucocerebrosidase from various production systems", CELL DEATH AND DISEASE, vol. 33, no. 5, 1 October 2013 (2013-10-01), pages 771 - 781+Supp, XP093082752, ISSN: 0144-8463, DOI: 10.1042/BSR20130081
GRACE, M.E. ; GRABOWSKI, G.A.: "Human acid ^2-glucosidase: Glycosylation is required for catalytic activity", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ELSEVIER, AMSTERDAM NL, vol. 168, no. 2, 30 April 1990 (1990-04-30), Amsterdam NL , pages 771 - 777, XP026787120, ISSN: 0006-291X, DOI: 10.1016/0006-291X(90)92388-G
BERG-FUSSMAN A ET AL: "Human acid beta-glucosidase. N-glycosylation site occupancy and the effect of glycosylation on enzymatic activity.", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, US, vol. 268, no. 20, 15 July 1993 (1993-07-15), US , pages 14861 - 14866, XP002436428, ISSN: 0021-9258
POL-FACHIN LAERCIO; SIEBERT MARINA; VERLI HUGO; SARAIVA-PEREIRA MARIA LUIZA: "Glycosylation is crucial for a proper catalytic site organization in human glucocerebrosidase", GLYCOCONJUGATE JOURNAL, CHAPMAN & HALL, BOSTON, vol. 33, no. 2, 29 March 2016 (2016-03-29), Boston , pages 237 - 244, XP035909603, ISSN: 0282-0080, DOI: 10.1007/s10719-016-9661-7
Attorney, Agent or Firm:
HATTA & ASSOCIATES (JP)
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