Title:
QPCR METHOD FOR DETECTION OF VARIANT THROUGH SIMULTANEOUS ANALYSIS OF MULTIPLE TARGETS
Document Type and Number:
WIPO Patent Application WO/2024/034796
Kind Code:
A1
Abstract:
The present invention relates to an economical and efficient variant detection method and kit that detects multiple mutation sites simultaneously with a probe emitting the same fluorescence, thereby reducing the possibility of false negatives and facilitating the simultaneous detection of many variants. The method and kit overcomes the disadvantage of low positive detection when using a hydrolyzed fluorescent probe with high specificity but low fluorescence signal value, and achieves high fluorescence signal values and low Ct values of qPCR through simultaneous detection of multiple mutation sites and thus can increase the sensitivity of detection. Additionally, the present invention pertains to a qPCR method and kit that employs probes that use the same type of fluorophores for multiple target genes within the same taxonomic group of organisms to boost detection sensitivity, resulting in amplified signal values.
Inventors:
KIM JAE JONG (KR)
LIM SI-KYU (KR)
KYUNG AYOUNG (KR)
LIM SI-KYU (KR)
KYUNG AYOUNG (KR)
Application Number:
PCT/KR2023/007259
Publication Date:
February 15, 2024
Filing Date:
May 26, 2023
Export Citation:
Assignee:
GENOTECH CORP (KR)
International Classes:
C12Q1/6848; C12Q1/6818; C12Q1/6827; C12Q1/70
Foreign References:
| CN113774169A | 2021-12-10 | |||
| CN113774168A | 2021-12-10 |
Other References:
KIM JAE JONG, PARK HYOUNG MIN, KYOUNG A. YOUNG, LIM SI-KYU, CHA SUN HO, LEE J. EUGENE, PARK BYOUNG CHUL: "Introduction of STexS Ⅱ: implementing ‘Com probes’ to enhance specificity of high Tm mutant probes", RESEARCH SQUARE, 6 July 2022 (2022-07-06), XP093128646, Retrieved from the Internet [retrieved on 20240207], DOI: 10.21203/rs.3.rs-1717463/v1
KIM JAE JONG, PARK HYOUNG-MIN, KYOUNG A YOUNG, LIM SI-KYU, CHA SUN HO, LEE J EUGENE, PARK BYOUNG CHUL, PARK CHUL: "Compact Method of SARS-CoV-2 PCR Detection Enhances Overall Process of Diagnosing Delta and Omicron Variants", SYSTEMATIC REVIEWS IN PHARMACY., vol. 13, no. 9, 17 June 2022 (2022-06-17), pages 630 - 634, XP093138186, DOI: 10.31858/0975-8453.13.9.630-634
NASEREDDIN ABDELMAJEED, GOLAN BERMAN HADAR, WOLF DANA, OIKNINE DJIAN ESTHER, ADAR SHEERA, KLUTSTEIN MICHAEL: "Identification of SARS-CoV-2 Variants of Concern Using Amplicon Next-Generation Sequencing", MICROBIOLOGY SPECTRUM, AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 10, no. 4, 31 August 2022 (2022-08-31), US , XP093138188, ISSN: 2165-0497, DOI: 10.1128/spectrum.00736-22
KIM JAE JONG, PARK HYOUNG-MIN, KYOUNG A YOUNG, LIM SI-KYU, CHA SUN HO, LEE J EUGENE, PARK BYOUNG CHUL, PARK CHUL: "Compact Method of SARS-CoV-2 PCR Detection Enhances Overall Process of Diagnosing Delta and Omicron Variants", SYSTEMATIC REVIEWS IN PHARMACY., vol. 13, no. 9, 17 June 2022 (2022-06-17), pages 630 - 634, XP093138186, DOI: 10.31858/0975-8453.13.9.630-634
NASEREDDIN ABDELMAJEED, GOLAN BERMAN HADAR, WOLF DANA, OIKNINE DJIAN ESTHER, ADAR SHEERA, KLUTSTEIN MICHAEL: "Identification of SARS-CoV-2 Variants of Concern Using Amplicon Next-Generation Sequencing", MICROBIOLOGY SPECTRUM, AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 10, no. 4, 31 August 2022 (2022-08-31), US , XP093138188, ISSN: 2165-0497, DOI: 10.1128/spectrum.00736-22
Attorney, Agent or Firm:
KANG, Seong-Hea (KR)
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