Title:
RAFFINOSE SYNTHETASE GENE, PROCESS FOR PRODUCING RAFFINOSE, AND TRANSFORMED PLANT
Document Type and Number:
WIPO Patent Application WO/1998/049273
Kind Code:
A1
Abstract:
A raffinose is produced by the action of a raffinose synthetase having the following properties on sucrose and galactinol: (1) activity and substrate specificity: raffinose is produced from sucrose and galactinol; (2) optimal pH: about 6-8; (3) optimal temp.: about 35-40 �C; (4) molecular weight: (i) molecular weight as measured by gel filtration chromatography: about 75-95 kDa; (ii) molecular weight as measured by polyacrylamide gel electrophoresis (native PAGE): about 90-100 kDa; and (iii) molecular weight as measured by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) under reducing conditions: about 90-100 kDa; (5) inhibition: it is inhibited by iodoacetamide, N-ethylmaleimide, and myoinositol.
Inventors:
OSUMI CHIEKO (JP)
NOZAKI JINSHI (JP)
KIDA TAKAO (JP)
NOZAKI JINSHI (JP)
KIDA TAKAO (JP)
Application Number:
PCT/JP1997/003879
Publication Date:
November 05, 1998
Filing Date:
October 24, 1997
Export Citation:
Assignee:
AJINOMOTO KK (JP)
OSUMI CHIEKO (JP)
NOZAKI JINSHI (JP)
KIDA TAKAO (JP)
OSUMI CHIEKO (JP)
NOZAKI JINSHI (JP)
KIDA TAKAO (JP)
International Classes:
C12N1/21; C12N9/10; C12N15/54; C12N15/82; (IPC1-7): C12N9/10; A01H5/00; C12N15/54
Domestic Patent References:
| WO1993002196A1 | 1993-02-04 |
Other References:
TRENDES IN GLYCOSCIENCE AND GLYCOTECHNOLOGY, Vol. 7, No. 34, (March 1995), HSHMOTO H. et al., "Synthesis of alpha-Galactosides with alpha-Galactosidase from Candida Guilliermondii H-404", pp. 149-158.
See also references of EP 0994186A4
See also references of EP 0994186A4
Attorney, Agent or Firm:
Toyama, Tsutomu (6th floor 4-10, Higashi Nihonbashi 3-chom, Chuo-ku Tokyo 103, JP)
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