Title:
RANDOM PROBE, PREPARATION METHOD THEREFOR AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/216938
Kind Code:
A1
Abstract:
The present disclosure relates to the technical field of molecular biology, and in particular to a random probe, a preparation method therefor and use thereof. The present disclosure provides a random probe, a preparation method therefor and use thereof. The preparation method comprises: using a transposase to perform random fragmentation on a target sequence; in the fragmentation process, adding transposase recognition sequences at both ends of a random target fragment obtained; then taking the obtained random target fragment as an amplification template and taking a sequence targeting the transposase recognition sequences as an amplification primer, and performing amplification in an amplification system with a quantity ratio of dUTP to dTTP+dUTP of 50%-100% to obtain the random probe targeting the target sequence. The random probe prepared by the method uniformly comprises bases U capable of serving as labeling sites.
Inventors:
HUANG MENG (CN)
YE XIN (CN)
YE XIN (CN)
Application Number:
PCT/CN2023/091732
Publication Date:
November 16, 2023
Filing Date:
April 28, 2023
Export Citation:
Assignee:
ZHUHAI SANMED BIOTECH LTD (CN)
International Classes:
C12Q1/6811; C12N15/11; C12Q1/6841
Foreign References:
| CN114891787A | 2022-08-12 | |||
| CN109825498A | 2019-05-31 | |||
| CN105506035A | 2016-04-20 | |||
| US20070128725A1 | 2007-06-07 | |||
| US20100120098A1 | 2010-05-13 | |||
| US20180237950A1 | 2018-08-23 |
Attorney, Agent or Firm:
CHOFN INTELLECTUAL PROPERTY (CN)
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