Title:
RECOMBINANT PRODUCTION OF PEPTIDES
Document Type and Number:
WIPO Patent Application WO/2010/139736
Kind Code:
A3
Abstract:
The present invention relates to repetitive self-assembling precursor proteins, nucleic acid sequences and expression constructs encoding the same, and to methods for recombinant production of peptides using such precursor proteins.
Inventors:
HUEMMERICH DANIEL (DE)
LIEBMANN BURGHARD (DE)
FEHR MARKUS (DE)
SCHWALB CARSTEN (DE)
BRUESER HEIKE (DE)
LIEBMANN BURGHARD (DE)
FEHR MARKUS (DE)
SCHWALB CARSTEN (DE)
BRUESER HEIKE (DE)
Application Number:
PCT/EP2010/057726
Publication Date:
March 10, 2011
Filing Date:
June 02, 2010
Export Citation:
Assignee:
BASF SE (DE)
HUEMMERICH DANIEL (DE)
LIEBMANN BURGHARD (DE)
FEHR MARKUS (DE)
SCHWALB CARSTEN (DE)
BRUESER HEIKE (DE)
HUEMMERICH DANIEL (DE)
LIEBMANN BURGHARD (DE)
FEHR MARKUS (DE)
SCHWALB CARSTEN (DE)
BRUESER HEIKE (DE)
International Classes:
C07K14/47; A61K38/16; C12N15/62; C12N15/74
Domestic Patent References:
| WO1998054336A1 | 1998-12-03 | |||
| WO2000031279A2 | 2000-06-02 | |||
| WO2008155304A1 | 2008-12-24 | |||
| WO2009080306A1 | 2009-07-02 |
Other References:
LEE J H ET AL: "Acidic Peptide-Mediated Expression of the Antimicrobial Peptide Buforin II as Tandem Repeats inEscherichia coli", PROTEIN EXPRESSION AND PURIFICATION, ACADEMIC PRESS, SAN DIEGO, CA, vol. 12, no. 1, 1 February 1998 (1998-02-01), pages 53 - 60, XP004447560, ISSN: 1046-5928, DOI: DOI:10.1006/PREP.1997.0814
HA-KUN KIM ET AL: "Expression of the cationic antimicrobial peptide lactoferricin fused with the anionic peptide in Escherichia coli", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, SPRINGER, BERLIN, DE, vol. 72, no. 2, 19 January 2006 (2006-01-19), pages 330 - 338, XP019421997, ISSN: 1432-0614, DOI: DOI:10.1007/S00253-005-0266-5
METLITSKAIA LUBA ET AL: "Recombinant antimicrobial peptides efficiently produced using novel cloning and purification processes.", BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY JUN 2004 LNKD- PUBMED:15154847, vol. 39, no. Pt 3, June 2004 (2004-06-01), pages 339 - 345, XP009142501, ISSN: 0885-4513
WANG YUN-QI ET AL: "High-level expression of acidic partner-mediated antimicrobial peptide from tandem genes in Escherichia coli.", APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2007 MAY-JUN LNKD- PUBMED:18025552, vol. 141, no. 2-3, May 2007 (2007-05-01), pages 203 - 213, XP009142497, ISSN: 0273-2289
VASSILEVSKI ALEXANDER A ET AL: "Antimicrobial peptide precursor structures suggest effective production strategies.", RECENT PATENTS ON INFLAMMATION & ALLERGY DRUG DISCOVERY 2008 LNKD- PUBMED:19075992, vol. 2, no. 1, 2008, pages 58 - 63, XP009142549, ISSN: 1872-213X
LI YIFENG: "Carrier proteins for fusion expression of antimicrobial peptides in Escherichia coli.", BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY SEP 2009 LNKD- PUBMED:19575694, vol. 54, no. 1, 6 July 2009 (2009-07-06), pages 1 - 9, XP009142490, ISSN: 1470-8744
LEE DONG GUN ET AL: "Structure and fungicidal activity of a synthetic antimicrobial peptide, P18, and its truncated peptides", BIOTECHNOLOGY LETTERS, KEW, SURREY, GB, vol. 26, no. 4, 1 February 2004 (2004-02-01), pages 337 - 341, XP002602555, ISSN: 0141-5492
HUEMMERICH D ET AL: "Primary structure elements of spider dragline silks and their contribution to protein solubility", BIOCHEMISTRY, AMERICAN CHEMICAL SOCIETY, US, vol. 43, no. 42, 26 October 2004 (2004-10-26), pages 13604 - 13612, XP002367100, ISSN: 0006-2960, DOI: DOI:10.1021/BI048983Q
HA-KUN KIM ET AL: "Expression of the cationic antimicrobial peptide lactoferricin fused with the anionic peptide in Escherichia coli", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, SPRINGER, BERLIN, DE, vol. 72, no. 2, 19 January 2006 (2006-01-19), pages 330 - 338, XP019421997, ISSN: 1432-0614, DOI: DOI:10.1007/S00253-005-0266-5
METLITSKAIA LUBA ET AL: "Recombinant antimicrobial peptides efficiently produced using novel cloning and purification processes.", BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY JUN 2004 LNKD- PUBMED:15154847, vol. 39, no. Pt 3, June 2004 (2004-06-01), pages 339 - 345, XP009142501, ISSN: 0885-4513
WANG YUN-QI ET AL: "High-level expression of acidic partner-mediated antimicrobial peptide from tandem genes in Escherichia coli.", APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2007 MAY-JUN LNKD- PUBMED:18025552, vol. 141, no. 2-3, May 2007 (2007-05-01), pages 203 - 213, XP009142497, ISSN: 0273-2289
VASSILEVSKI ALEXANDER A ET AL: "Antimicrobial peptide precursor structures suggest effective production strategies.", RECENT PATENTS ON INFLAMMATION & ALLERGY DRUG DISCOVERY 2008 LNKD- PUBMED:19075992, vol. 2, no. 1, 2008, pages 58 - 63, XP009142549, ISSN: 1872-213X
LI YIFENG: "Carrier proteins for fusion expression of antimicrobial peptides in Escherichia coli.", BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY SEP 2009 LNKD- PUBMED:19575694, vol. 54, no. 1, 6 July 2009 (2009-07-06), pages 1 - 9, XP009142490, ISSN: 1470-8744
LEE DONG GUN ET AL: "Structure and fungicidal activity of a synthetic antimicrobial peptide, P18, and its truncated peptides", BIOTECHNOLOGY LETTERS, KEW, SURREY, GB, vol. 26, no. 4, 1 February 2004 (2004-02-01), pages 337 - 341, XP002602555, ISSN: 0141-5492
HUEMMERICH D ET AL: "Primary structure elements of spider dragline silks and their contribution to protein solubility", BIOCHEMISTRY, AMERICAN CHEMICAL SOCIETY, US, vol. 43, no. 42, 26 October 2004 (2004-10-26), pages 13604 - 13612, XP002367100, ISSN: 0006-2960, DOI: DOI:10.1021/BI048983Q
Attorney, Agent or Firm:
PATENTANWÄLTE REITSTÖTTER KINZEBACH (München, DE)
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