Title:
RECOMBINANT STRAIN FOR PRODUCING L-AMINO ACID, CONSTRUCTION METHOD THEREFOR, AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2021/248902
Kind Code:
A1
Abstract:
Provided is a bacterium for producing L-amino acid. The bacterium has improved expression of a polynucleotide for encoding a protein represented by SEQ ID NO:3 and improved expression of a polynucleotide for encoding a protein represented by SEQ ID NO:31, and/or has mutations in bases at positions -45bp and -47bp of a promotor region represented by SEQ ID NO:57. Also provided are a polynucleotide, proteins encoded thereby, a recombinant carrier and a recombinant strain comprising a polynucleotide sequence, and a method for producing L-amino acid. The polynucleotide encodes a protein which is represented by SEQ ID NO:3 and has arginine at position 334 substituted by a terminator or encodes a protein which is represented by SEQ ID NO:31 and has tyrosine at position 592 substituted by phenylalanine, or is formed by mutations in bases at positions -45bp and -47bp of a promotor region represented by SEQ ID NO:57.
Inventors:
WEI AIYING (CN)
MENG GANG (CN)
ZHOU XIAOQUN (CN)
ZHAO CHUNGUANG (CN)
MA FENGYONG (CN)
JIA HUIPING (CN)
YANG LIPENG (CN)
SU HOUBO (CN)
GUO XIAOWEI (CN)
TIAN BIN (CN)
GAO XIAOHANG (CN)
MENG GANG (CN)
ZHOU XIAOQUN (CN)
ZHAO CHUNGUANG (CN)
MA FENGYONG (CN)
JIA HUIPING (CN)
YANG LIPENG (CN)
SU HOUBO (CN)
GUO XIAOWEI (CN)
TIAN BIN (CN)
GAO XIAOHANG (CN)
Application Number:
PCT/CN2020/142133
Publication Date:
December 16, 2021
Filing Date:
December 31, 2020
Export Citation:
Assignee:
INNER MONGOLIA EPPEN BIOTECH CO LTD (CN)
International Classes:
C12N15/54; C07K14/34; C12N1/21; C12N15/10; C12N15/113; C12N15/31; C12N15/77; C12P13/08; C12P13/14
Domestic Patent References:
| WO2014121669A1 | 2014-08-14 |
Foreign References:
| CN111961635A | 2020-11-20 | |||
| CN111909944A | 2020-11-10 | |||
| CN110951662A | 2020-04-03 | |||
| US7332310B2 | 2008-02-19 | |||
| CN202011105063A | 2020-10-15 | |||
| CN202010790887A | 2020-08-07 | |||
| CN202010514037A | 2020-06-08 | |||
| CN106367432A | 2017-02-01 | |||
| CN110951662A | 2020-04-03 |
Other References:
WEI AIYING, GE YUPING: "L-Lysine: From Metabolic Pathways to Industrial Production", BULLETIN OF FERMENTATION SCIENCE AND TECHNOLOGY, ZHEJIANG UNIVERSITY OF TECHNOLOGY, CN, vol. 40, no. 2, 30 April 2011 (2011-04-30), CN , pages 31 - 34, XP055879306, ISSN: 1674-2214, DOI: 10.16774/j.cnki.issn.1674-2214.2011.02.014
BARKER, M.M. GAAL, T. JOSAITIS, C.A. GOURSE, R.L.: "Mechanism of regulation of transcription initiation by ppGpp. I. Effects of ppGpp on transcription initiation in vivo and in vitro", JOURNAL OF MOLECULAR BIOLOGY, ACADEMIC PRESS, UNITED KINGDOM, vol. 305, no. 4, 26 January 2001 (2001-01-26), United Kingdom , pages 673 - 688, XP004470907, ISSN: 0022-2836, DOI: 10.1006/jmbi.2000.4327
PÁTEK MIROSLAV, NEŠVERA JAN: "Sigma factors and promoters in Corynebacterium glutamicum", JOURNAL OF BIOTECHNOLOGY, vol. 154, no. 2-3, 1 July 2011 (2011-07-01), Amsterdam NL , pages 101 - 113, XP093067626, ISSN: 0168-1656, DOI: 10.1016/j.jbiotec.2011.01.017
BARKER, M.M. GAAL, T. JOSAITIS, C.A. GOURSE, R.L.: "Mechanism of regulation of transcription initiation by ppGpp. I. Effects of ppGpp on transcription initiation in vivo and in vitro", JOURNAL OF MOLECULAR BIOLOGY, ACADEMIC PRESS, UNITED KINGDOM, vol. 305, no. 4, 26 January 2001 (2001-01-26), United Kingdom , pages 673 - 688, XP004470907, ISSN: 0022-2836, DOI: 10.1006/jmbi.2000.4327
PÁTEK MIROSLAV, NEŠVERA JAN: "Sigma factors and promoters in Corynebacterium glutamicum", JOURNAL OF BIOTECHNOLOGY, vol. 154, no. 2-3, 1 July 2011 (2011-07-01), Amsterdam NL , pages 101 - 113, XP093067626, ISSN: 0168-1656, DOI: 10.1016/j.jbiotec.2011.01.017
Attorney, Agent or Firm:
BEIJING ORIGINTELLIGENCE IP LAW FIRM (CN)
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