Title:
RECOMBINANT TYPE-I HUMANIZED COLLAGEN POLYPEPTIDE, AND PREPARATION METHOD THEREFOR AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/024552
Kind Code:
A1
Abstract:
Disclosed in the present invention are a recombinant type-I humanized collagen polypeptide, and a preparation method therefor and the use thereof. The recombinant type-I humanized collagen polypeptide provided by the present invention comprises n repeats of a sequence as shown in SEQ ID NO: 1, wherein n is an integer greater than or equal to 1; the repeated sequences are directly linked when n is an integer greater than or equal to 2; and optionally, the N-terminus of the recombinant type-I humanized collagen polypeptide contains an amino acid sequence capable of being cleaved off by the TEV protease. The recombinant type-I humanized collagen polypeptide provided by the present invention has an activity of promoting cell adhesion, the recombinant protein has an amino acid sequence selected from the amino acid sequence of natural collagen and does not generate immune response when applied to the human body, the preparation method therefor is simple, and high-yield collagen can be obtained at a low cost.
Inventors:
YANG XIA (CN)
LU CHENYANG (CN)
LAN XIAOBIN (CN)
HE ZHENRUI (CN)
WANG JIAN (CN)
WANG LINGLING (CN)
LU CHENYANG (CN)
LAN XIAOBIN (CN)
HE ZHENRUI (CN)
WANG JIAN (CN)
WANG LINGLING (CN)
Application Number:
PCT/CN2022/089283
Publication Date:
March 02, 2023
Filing Date:
April 26, 2022
Export Citation:
Assignee:
SHANXI JINBO BIO PHARMACEUTICAL CO LTD (CN)
International Classes:
C07K14/78; A23L33/18; A61K8/65; A61P43/00; A61Q19/00; C12N15/70
Foreign References:
| CN113621052A | 2021-11-09 | |||
| CN109293783A | 2019-02-01 | |||
| CN109593126A | 2019-04-09 | |||
| CN109575126A | 2019-04-05 | |||
| CN109535232A | 2019-03-29 | |||
| CN201811438582A | 2018-11-28 | |||
| CN201811254050A | 2018-10-25 |
Other References:
JOURNAL OF BIOCHEMISTRY, vol. 136, 2004, pages 643 - 649
JUMING YAOSATOSHI YANAGISAWATETSUO ASAKURA: "Design, Expression and Characterization of Collagen-Like Proteins Based on the Cell Adhesive and Crosslinking Sequences Derived from Native Collagens", J BIOCHEM., vol. 136, 2004, pages 643 - 649, XP055677659, DOI: 10.1093/jb/mvh172
JUMING YAOSATOSHI YANAGISAWATETSUO ASAKURA: "Design, Expression and Characterization of Collagen-Like Proteins Based on the Cell Adhesive and Crosslinking Sequences Derived from Native Collagens", J BIOCHEM., vol. 136, 2004, pages 643 - 649, XP055677659, DOI: 10.1093/jb/mvh172
Attorney, Agent or Firm:
LINDA LIU & PARTNERS (CN)
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