Title:
RECOMBINANT VECTOR FOR PRODUCING CARDIAC HYPERTROPHY ANIMAL MODEL, CARDIAC HYPERTROPHY ANIMAL MODEL TRANSFORMED WITH RECOMBINANT VECTOR, AND PRODUCTION METHOD THEREFOR
Document Type and Number:
WIPO Patent Application WO/2020/085838
Kind Code:
A1
Abstract:
The present invention relates to a recombinant vector for producing a cardiac hypertrophy animal model, a cardiac hypertrophy animal model transformed with the recombinant vector, and a production method therefor. When a recombinant vector according to the present invention is specifically expressed in mouse cardiomyocytes, only non-canonical G:A wobble base sequence target sites of miRNA are recognized and inhibited, so that a biological function of increasing the size of cardiomyocytes is exhibited so as to induce cardiac hypertrophy, and thus an animal model in which cardiac hypertrophy has been induced is expected to be produced so as to be effectively used for studying the progression, pathology and physiological mechanism of heart diseases including cardiac hypertrophy at the individual level.
Inventors:
CHI SUNG WOOK (KR)
SEOK HEE YOUNG (KR)
SEOK HEE YOUNG (KR)
Application Number:
PCT/KR2019/014133
Publication Date:
April 30, 2020
Filing Date:
October 25, 2019
Export Citation:
Assignee:
UNIV KOREA RES & BUS FOUND (KR)
International Classes:
C12N15/85; A01K67/027; G01N33/50
Foreign References:
| US6201165B1 | 2001-03-13 | |||
| KR20180103258A | 2018-09-19 |
Other References:
BRANCATI, G.: "An interplay of miRNA abundance and target site architecture determines miRNA activity and specificity", NUCLEIC ACIDS RESEARCH, 21 March 2018 (2018-03-21), pages 3259 - 3269
CALLIS, T. E.: "MicroRNA-208a is a regulator of cardiac hypertrophy and conduction in mice", THE JOURNAL OF CLINICAL INVESTIGATION, vol. 11 9, no. 9, September 2009 (2009-09-01), pages 2772 - 2786, XP055090003, DOI: 10.1172/JCI36154
WANG, J.: "Overview of microRNAs in cardiac hypertrophy, fibrosis, and apoptosis", INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 17, no. 5, 18 May 2016 (2016-05-18), pages 749, XP055708754
DONG, D.: "Role of microRNAs in cardiac hypertrophy, myocardial fibrosis and heart failure", ACTA PHARMACEUTICA SINICA B, vol. 1, no. 1, 1 June 2011 (2011-06-01), pages 1 - 7, XP055708756
CALLIS, T. E.: "MicroRNA-208a is a regulator of cardiac hypertrophy and conduction in mice", THE JOURNAL OF CLINICAL INVESTIGATION, vol. 11 9, no. 9, September 2009 (2009-09-01), pages 2772 - 2786, XP055090003, DOI: 10.1172/JCI36154
WANG, J.: "Overview of microRNAs in cardiac hypertrophy, fibrosis, and apoptosis", INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 17, no. 5, 18 May 2016 (2016-05-18), pages 749, XP055708754
DONG, D.: "Role of microRNAs in cardiac hypertrophy, myocardial fibrosis and heart failure", ACTA PHARMACEUTICA SINICA B, vol. 1, no. 1, 1 June 2011 (2011-06-01), pages 1 - 7, XP055708756
Attorney, Agent or Firm:
LEE, Myoung-Jin (KR)
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