Title:
REGULARLY ARRANGED SPHEROIDS HAVING UNIFORM SIZE, AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2017/150366
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing a screening method for accurately evaluating a large quantity of candidate compounds, which uses a group of spheroids that are uniform in size and position. This screening method for substances which affect spheroid formation includes: a step (1) in which cells are seeded in a density effective for spheroid formation, in a plate (a well plate) having, regularly arranged therein, a plurality of wells each having a U-shaped bottom exhibiting low adsorptivity, and the cells are cultured in the plurality of wells; a step (2) in which the cells are brought into contact with test substances; and a step (3) in which it is observed whether the cells which have been brought into contact with the test substances form spheroids, and the effect of the test substances on spheroid formation is evaluated using the obtained observation results as an index.
Inventors:
YONEMITSU YOSHIKAZU (JP)
HARADA YUI (JP)
MORODOMI YOSUKE (JP)
TERAISHI KOJI (JP)
HARADA YUI (JP)
MORODOMI YOSUKE (JP)
TERAISHI KOJI (JP)
Application Number:
PCT/JP2017/007029
Publication Date:
September 08, 2017
Filing Date:
February 24, 2017
Export Citation:
Assignee:
YONEMITSU YOSHIKAZU (JP)
GAIA BIOMEDICINE INC (JP)
GAIA BIOMEDICINE INC (JP)
International Classes:
C12N5/07; C12Q1/02; C12M3/00
Domestic Patent References:
| WO2011083768A1 | 2011-07-14 | |||
| WO2014196204A1 | 2014-12-11 | |||
| WO2014179196A1 | 2014-11-06 | |||
| WO2014038025A1 | 2014-03-13 | |||
| WO2003058251A2 | 2003-07-17 | |||
| WO2014038025A1 | 2014-03-13 | |||
| WO2008123614A1 | 2008-10-16 |
Foreign References:
| JPH08131153A | 1996-05-28 | |||
| JP2009050194A | 2009-03-12 | |||
| JP2010094045A | 2010-04-30 | |||
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| JP2015033384A | 2015-02-19 | |||
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Other References:
ZHANG. J.H. ET AL., J. BIOMOL. SCREEN., vol. 4, 1999, pages 67 - 73
IKADA, Y: "Surface modification of polymers for medical applications", BIOMATERIALS, vol. 15, no. 10, 1994, pages 725 - 736, XP024142207, DOI: doi:10.1016/0142-9612(94)90025-6
Y KASAGI ET AL.: "Peritoneal Dissemination Requires an Spl-Dependent CXCR4/CXCL12 Signaling Axis and Extracellular Matrix-Directed Spheroid Formation", CANCER RES., vol. 76, no. 2, 15 January 2016 (2016-01-15), pages 347 - 357
Y IKEDA ET AL.: "Simian immunodeficiency virus-based lentivirus vector for retinal gene transfer: a preclinical safety study in adult rats", GENE THERAPY, vol. 10, 2003, pages 1161 - 1169, XP003004709, DOI: doi:10.1038/sj.gt.3301973
See also references of EP 3425043A4
IKADA, Y: "Surface modification of polymers for medical applications", BIOMATERIALS, vol. 15, no. 10, 1994, pages 725 - 736, XP024142207, DOI: doi:10.1016/0142-9612(94)90025-6
Y KASAGI ET AL.: "Peritoneal Dissemination Requires an Spl-Dependent CXCR4/CXCL12 Signaling Axis and Extracellular Matrix-Directed Spheroid Formation", CANCER RES., vol. 76, no. 2, 15 January 2016 (2016-01-15), pages 347 - 357
Y IKEDA ET AL.: "Simian immunodeficiency virus-based lentivirus vector for retinal gene transfer: a preclinical safety study in adult rats", GENE THERAPY, vol. 10, 2003, pages 1161 - 1169, XP003004709, DOI: doi:10.1038/sj.gt.3301973
See also references of EP 3425043A4
Attorney, Agent or Firm:
SIKS & CO. (JP)
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