Title:
RELAXATION NUCLEAR MAGNETIC RESONANCE METHOD FOR MEASURING GLUCOSE CONTENT IN LIQUID BIOLOGICAL SAMPLE
Document Type and Number:
WIPO Patent Application WO/2022/099972
Kind Code:
A1
Abstract:
A relaxation nuclear magnetic resonance method for measuring glucose content in a liquid biological sample, wherein glucose oxidase and acidified potassium permanganate are used as reaction substrates, and the glucose content in a measured sample can be calculated by detecting the change of relaxation time by means of 1H-NMR. The method specifically comprises: step a: adding absolute ethyl alcohol or other equivalent organic reagents to a sample being measured in a certain proportion, mixing them well and letting the mixture stand still to denature and precipitate interfering proteins in the sample; step b: taking the supernatant and evaporating residual organic reagents; step c: respectively adding a detection solution and a control solution to a group A and a group B for reaction; step d: measuring a relaxation difference between the groups A and B by using a nuclear magnetic resonance instrument; and step e: calculating the glucose content in the measured sample according to the measured relaxation time difference and a known model under the sample category. By means of the relaxation nuclear magnetic resonance method, the glucose content in various liquid biological samples can be effectively measured, helping to further study the application of nuclear magnetic resonance technology in the direction of biological sugar detection.
Inventors:
YI HONG (CN)
LU RONGSHENG (CN)
CHEN YI (CN)
NI ZHONGHUA (CN)
JIANG XIAOWEN (CN)
WU ZHENGXIU (CN)
LU RONGSHENG (CN)
CHEN YI (CN)
NI ZHONGHUA (CN)
JIANG XIAOWEN (CN)
WU ZHENGXIU (CN)
Application Number:
PCT/CN2021/083125
Publication Date:
May 19, 2022
Filing Date:
March 26, 2021
Export Citation:
Assignee:
UNIV SOUTHEAST (CN)
International Classes:
G01N24/08
Foreign References:
| CN112067647A | 2020-12-11 | |||
| CN108333536A | 2018-07-27 | |||
| CN106198596A | 2016-12-07 | |||
| US6163154A | 2000-12-19 | |||
| JP2006023312A | 2006-01-26 |
Other References:
XIAO, YAHWEH, GUAN JIANQIU, JING FENGYING: "Quantitative Determination of Glucose and Fructose Contents by Carbon-13 Nuclear Magnetic Resonance", CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,, vol. 20, no. 3, 31 December 1992 (1992-12-31), CN , pages 255 - 258, XP009536784, ISSN: 0253-3820
Attorney, Agent or Firm:
JW IP LAW FIRM (CN)
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