Title:
RICE MALE FERTILITY REGULATORY GENE, MUTANT OF RICE MALE FERTILITY REGULATORY GENE, USE THEREOF AND METHOD FOR REGULATING RICE FERTILITY
Document Type and Number:
WIPO Patent Application WO/2021/244007
Kind Code:
A1
Abstract:
The present invention relates to the technical field of biology, in particular to a rice male fertility regulatory gene, the use thereof and a method for regulating rice fertility by means of using CRISPR-Cas9, and a mutant of the rice male fertility regulatory gene and a molecular marker and the use thereof. The present invention provides a rice gene GMS2 with the functions of regulating male germ cell development in rice and pollen fertility. The mutant of a rice male fertility regulatory protein GMS2 provided by the present invention can make rice pollen completely sterile, resulting in rice male sterility. The rice gene GMS2 and the mutant thereof, provided by the present invention, can be used in the sterile seed production and manufacturing of rice hybrids, thereby possessing both great application value and economic value.
Inventors:
LONG TUAN (CN)
TANG JIE (CN)
LI JIALIN (CN)
WU CHUNYU (CN)
LIU HAO (CN)
LI YANQUN (CN)
HAN XIAOBIN (CN)
ZENG XIANG (CN)
LI XINPENG (CN)
AN BAOGUANG (CN)
WU YONGZHONG (CN)
HUANG PEIJIN (CN)
TANG JIE (CN)
LI JIALIN (CN)
WU CHUNYU (CN)
LIU HAO (CN)
LI YANQUN (CN)
HAN XIAOBIN (CN)
ZENG XIANG (CN)
LI XINPENG (CN)
AN BAOGUANG (CN)
WU YONGZHONG (CN)
HUANG PEIJIN (CN)
Application Number:
PCT/CN2020/137145
Publication Date:
December 09, 2021
Filing Date:
December 17, 2020
Export Citation:
Assignee:
HAINAN BOLIAN RICE GENE TECH CO LTD (CN)
International Classes:
C07K14/415; A01H5/00; C12N15/29; C12N15/82
Foreign References:
| CN110511945A | 2019-11-29 | |||
| CN105002191A | 2015-10-28 | |||
| US20040123343A1 | 2004-06-24 |
Other References:
DATABASE Protein 23 March 2015 (2015-03-23), ANONYMOUS: "hypothetical protein OsI_17056 [Oryza sativa Indica Group]", XP055877173, retrieved from Genbank Database accession no. EAY95238
DATABASE Protein 11 May 2017 (2017-05-11), ANONYMOUS: "fasciclin-like arabinogalactan protein 10 [Arabidopsis lyrata subsp. lyrata]", XP055877177, retrieved from Genbank Database accession no. XP_020880420
DATABASE UniProtKB 3 April 2013 (2013-04-03), ANONYMOUS: "M0RNY9_MUSAM ", XP009532617
DATABASE Protein 6 April 2021 (2021-04-06), ANONYMOUS: "fasciclin-like arabinogalactan protein 8 [Oryza brachyantha]", XP055877189, retrieved from Genbank Database accession no. XP_006652672
DATABASE Protein 13 June 2017 (2017-06-13), ANONYMOUS: "fasciclin-like arabinogalactan protein 8 [Sorghum bicolor]", XP055877191, retrieved from Genbank Database accession no. XP_002448368
DATABASE Protein 2 July 2020 (2020-07-02), ANONYMOUS: "fasciclin-like arabinogalactan protein 10 precursor [Zea mays]", XP055877195, retrieved from Genbank Database accession no. NP_001151356
DATABASE Protein 25 October 2016 (2016-10-25), ANONYMOUS: "PREDICTED: fasciclin-like arabinogalactan protein 8 [Musa acuminata subsp. malaccensis]", XP055877198, retrieved from Genbank Database accession no. XP_009382277
DATABASE Protein 29 November 2019 (2019-11-29), ANONYMOUS: "fasciclin-like arabinogalactan protein 8 [Hordeum vulgare]", XP055877201, retrieved from Genbank Database accession no. KAE8775436
DATABASE Protein 10 December 2008 (2008-12-10), ANONYMOUS: "fasciclin-like arabinogalactan protein 10 precursor [Zea mays]", XP055877207, retrieved from Genbank Database accession no. ACG42498
DATABASE Protein 13 October 2017 (2017-10-13), ANONYMOUS: "fasciclin-like arabinogalactan protein 8 [Setaria italica]", XP055877215, retrieved from Genbank Database accession no. XP_004976555
DATABASE Nucleotide 4 December 2008 (2008-12-04), ANONYMOUS: "Oryza sativa Japonica Group cDNA, clone: J075088H12, full insert sequence", XP055877222, retrieved from Genbank Database accession no. AK241942
JUN LI,MIAO YU,LING-LING GENG,JIE ZHAO: "The Fasciclin-like Arabinogalactan Protein Gene, FLA3, is Involved in Microspore Development of Arabidopsis", THE PLANT JOURNAL, vol. 64, no. 3, 1 November 2010 (2010-11-01), GB, pages 482 - 497, XP055877224, ISSN: 0960-7412, DOI: 0.1111/j.1365-313X.2010.04344.x
RHEE SUN-JU, KWON TAEHYUNG, SEO MINSEOK, JANG YOON JEONG, SIM TAE YONG, CHO SEOAE, HAN SANG-WOOK, LEE GUNG PYO: "De Novo-based Transcriptome Profiling of Male-sterile and Fertile Watermelon Lines", PLOS ONE, vol. 12, no. 11, 2 November 2017 (2017-11-02), pages 1 - 17, XP055877227, DOI: 10.1371/journal.pone.0187147
DATABASE Protein 11 May 2017 (2017-05-11), ANONYMOUS: "fasciclin-like arabinogalactan protein 10 [Arabidopsis lyrata subsp. lyrata]", XP055877177, retrieved from Genbank Database accession no. XP_020880420
DATABASE UniProtKB 3 April 2013 (2013-04-03), ANONYMOUS: "M0RNY9_MUSAM ", XP009532617
DATABASE Protein 6 April 2021 (2021-04-06), ANONYMOUS: "fasciclin-like arabinogalactan protein 8 [Oryza brachyantha]", XP055877189, retrieved from Genbank Database accession no. XP_006652672
DATABASE Protein 13 June 2017 (2017-06-13), ANONYMOUS: "fasciclin-like arabinogalactan protein 8 [Sorghum bicolor]", XP055877191, retrieved from Genbank Database accession no. XP_002448368
DATABASE Protein 2 July 2020 (2020-07-02), ANONYMOUS: "fasciclin-like arabinogalactan protein 10 precursor [Zea mays]", XP055877195, retrieved from Genbank Database accession no. NP_001151356
DATABASE Protein 25 October 2016 (2016-10-25), ANONYMOUS: "PREDICTED: fasciclin-like arabinogalactan protein 8 [Musa acuminata subsp. malaccensis]", XP055877198, retrieved from Genbank Database accession no. XP_009382277
DATABASE Protein 29 November 2019 (2019-11-29), ANONYMOUS: "fasciclin-like arabinogalactan protein 8 [Hordeum vulgare]", XP055877201, retrieved from Genbank Database accession no. KAE8775436
DATABASE Protein 10 December 2008 (2008-12-10), ANONYMOUS: "fasciclin-like arabinogalactan protein 10 precursor [Zea mays]", XP055877207, retrieved from Genbank Database accession no. ACG42498
DATABASE Protein 13 October 2017 (2017-10-13), ANONYMOUS: "fasciclin-like arabinogalactan protein 8 [Setaria italica]", XP055877215, retrieved from Genbank Database accession no. XP_004976555
DATABASE Nucleotide 4 December 2008 (2008-12-04), ANONYMOUS: "Oryza sativa Japonica Group cDNA, clone: J075088H12, full insert sequence", XP055877222, retrieved from Genbank Database accession no. AK241942
JUN LI,MIAO YU,LING-LING GENG,JIE ZHAO: "The Fasciclin-like Arabinogalactan Protein Gene, FLA3, is Involved in Microspore Development of Arabidopsis", THE PLANT JOURNAL, vol. 64, no. 3, 1 November 2010 (2010-11-01), GB, pages 482 - 497, XP055877224, ISSN: 0960-7412, DOI: 0.1111/j.1365-313X.2010.04344.x
RHEE SUN-JU, KWON TAEHYUNG, SEO MINSEOK, JANG YOON JEONG, SIM TAE YONG, CHO SEOAE, HAN SANG-WOOK, LEE GUNG PYO: "De Novo-based Transcriptome Profiling of Male-sterile and Fertile Watermelon Lines", PLOS ONE, vol. 12, no. 11, 2 November 2017 (2017-11-02), pages 1 - 17, XP055877227, DOI: 10.1371/journal.pone.0187147
Attorney, Agent or Firm:
CN-KNOWHOW INTELLECTUAL PROPERTY AGENT LIMITED (CN)
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